Abstract:
BACKGROUND:Cationic antimicrobial peptides (CAPs) with antitumor activity constitute a promising group of novel anticancer agents. These peptides induce lysis of cancer cells through interactions with the plasma membrane. It is not known which cancer cell membrane components influence their susceptibility to CAPs. We have previously shown that CAPs interact with the two glycosaminoglycans (GAGs), heparan sulfate (HS) and chondroitin sulfate (CS), which are present on the surface of most cells. The purpose of this study was to investigate the role of the two GAGs in the cytotoxic activity of CAPs. METHODS:Various cell lines, expressing different levels of cell surface GAGs, were exposed to bovine lactoferricin (LfcinB) and the designer peptide, KW5. The cytotoxic effect of the peptides was investigated by use of the colorimetric MTT viability assay. The cytotoxic effect on wild type CHO cells, expressing normal amounts of GAGs on the cell surface, and the mutant pgsA-745, that has no expression of GAGs on the cell surface, was also investigated. RESULTS:We show that cells not expressing HS were more susceptible to CAPs than cells expressing HS at the cell surface. Further, exogenously added heparin inhibited the cytotoxic effect of the peptides. Chondroitin sulfate had no effect on the cytotoxic activity of KW5 and only minor effects on LfcinB cytotoxicity. CONCLUSION:Our results show for the first time that negatively charged molecules at the surface of cancer cells inhibit the cytotoxic activity of CAPs. Our results indicate that HS at the surface of cancer cells sequesters CAPs away from the phospholipid bilayer and thereby impede their ability to induce cytolysis.
journal_name
BMC Cancerjournal_title
BMC cancerauthors
Fadnes B,Rekdal O,Uhlin-Hansen Ldoi
10.1186/1471-2407-9-183subject
Has Abstractpub_date
2009-06-15 00:00:00pages
183issn
1471-2407pii
1471-2407-9-183journal_volume
9pub_type
杂志文章相关文献
BMC CANCER文献大全abstract:BACKGROUND:Increasing evidence suggests that alterations in mitochondrial DNA (mtDNA) content may be implicated in the tumorigenesis of several malignancies. However, the association between mtDNA content in peripheral blood lymphocytes (PBLs) and glioma risk has not been investigated. METHODS:Real-time PCR was used t...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-14-680
更新日期:2014-09-19 00:00:00
abstract::After publication of the original article [1] the authors found that the article contained an incorrect version of Fig. 4. This does not affect the results and conclusions of the article. ...
journal_title:BMC cancer
pub_type: 杂志文章,已发布勘误
doi:10.1186/s12885-017-3766-7
更新日期:2018-01-16 00:00:00
abstract:BACKGOUND:Oropharyngeal cancer (OPC) associated with human papilloma virus (HPV OPC) shows better treatment outcomes than non-HPV OPC. We investigated the expression of p53, β-tubulin, bcl-2 and ERCC 1, which are well-known biomarkers to predict the chemotherapy response, according to HPV status in OPC patients. METHO...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-14-824
更新日期:2014-11-07 00:00:00
abstract:BACKGROUND:It has been shown in several studies that survival in cancer patients who were operated on by a high-volume surgeon was better. Why then do all patients not benefit from treatment by these experienced surgeons? The aim of our work was to study the hypothesis that in breast cancer, geographical isolation and ...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-12-351
更新日期:2012-08-13 00:00:00
abstract:BACKGROUND:Androgen deprivation therapy (ADT) is a cornerstone in the treatment of advanced prostate cancer. Adverse musculoskeletal and cardiovascular effects of ADT are widely reported and investigations into the potential of exercise to ameliorate the effects of treatment are warranted. The 'Football Club (FC) Prost...
journal_title:BMC cancer
pub_type: 杂志文章,随机对照试验
doi:10.1186/1471-2407-13-595
更新日期:2013-12-13 00:00:00
abstract:BACKGROUND:The Src homology phosphotyrosyl phosphatase 2 (SHP2) is a positive effector of cell growth and survival signaling as well transformation induced by multiple tyrosine kinase oncogenes. Since the basal-like and triple-negative breast cancer (BTBC) is characterized by dysregulation of multiple tyrosine kinase o...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-015-1131-2
更新日期:2015-03-08 00:00:00
abstract:BACKGROUND:Mantle Cell Lymphoma (MCL) is often associated with progression, temporary response to therapy and a high relapse rate over time resulting in a poor long-term prognosis. Because MCL is classified as an incurable disease, therapeutic resistance is of great interest. However, knowledge about the biological mec...
journal_title:BMC cancer
pub_type: 杂志文章,多中心研究
doi:10.1186/s12885-016-2844-6
更新日期:2016-10-14 00:00:00
abstract:BACKGROUND:With the advent of novel drugs improved overall survival in patients with multiple myeloma, including patients who received up-front autologous stem cell transplantation (ASCT), has been reported from several centers. Here we report on overall survival in a population-based cohort of patients receiving ASCT ...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-018-4722-x
更新日期:2018-08-08 00:00:00
abstract:BACKGROUND:To obtain high-yield histological samples by targeted prostate cancer (PCa) biopsy is the current trend compared with transrectal ultrasound (TRUS)-guided systematic histological biopsy, which is regarded as the gold standard for prostate cancer (PCa) diagnosis. In this paper, we present a targeted PCa imagi...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-020-06801-9
更新日期:2020-05-14 00:00:00
abstract:BACKGROUND:Most currently known breast cancer predisposition genes play a role in DNA repair by homologous recombination. Recent studies conducted on RAD51 paralogs, involved in the same DNA repair pathway, have identified rare germline mutations conferring breast and/or ovarian cancer predisposition in the RAD51C, RAD...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-13-484
更新日期:2013-10-19 00:00:00
abstract:BACKGROUND:Sentinel lymph node (SLN) biopsy is an effective tool for axillary staging in patients with invasive breast cancer. This procedure has been recently proposed as part of the treatment for patients with ductal carcinoma in situ (DCIS), because cases of undetected invasive foci and nodal metastases occasionally...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-5-28
更新日期:2005-03-11 00:00:00
abstract:BACKGROUND:Long non-coding RNAs have been shown to have critical regulatory roles in cancer biology. However, the contributions of lncRNAs to gastric cancer remain largely unknown. METHODS:The differential expression of lncRNAs in gastric cancer and paired non-cancerous tissues were identified by microarray and valida...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-14-932
更新日期:2014-12-11 00:00:00
abstract:BACKGROUND:This study aimed to identify potential predictive factors for the survival of advanced non small-cell lung cancer (NSCLC) patients undergoing first-line platinum-based chemotherapy. METHODS:A total of 270 advanced NSCLC patients who underwent first-line platinum-based chemotherapy from June, 2011 to June, 2...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-019-5490-y
更新日期:2019-03-29 00:00:00
abstract:BACKGROUND:Increased mammographic breast density is a moderate risk factor for breast cancer. Different scales have been proposed for classifying mammographic density. This study sought to assess intra-rater agreement for the most widely used scales (Wolfe, Tabár, BI-RADS and Boyd) and compare them in terms of classify...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-10-485
更新日期:2010-09-13 00:00:00
abstract:BACKGROUND:Survival rates of lung cancer remains poor and the impact of comorbidities on the prognosis is discussed. The objective of this study was to assess if the Charlson Comorbidity Index (CCI) was associated with 8-year survival rates by histological type. METHODS:A cohort study was conducted using randomly sele...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-018-5000-7
更新日期:2018-11-12 00:00:00
abstract:BACKGROUND:A universal hallmark of cancer cells is the change in their glycosylation phenotype. One of the most frequent alterations in the normal glycosylation pattern observed during carcinogenesis is the enhancement of α(1,6)linked fucose residues of glycoproteins, due to the up-regulation of the α(1,6)fucosyltransf...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-11-508
更新日期:2011-12-07 00:00:00
abstract:BACKGROUND:Whether perioperative blood transfusions (PBTs) adversely influence oncological outcomes for intrahepatic cholangiocarcinoma (ICC) patients after curative resection remains undetermined. METHODS:Of the 605 patients who underwent curative liver resection for ICC between 2000 and 2012, 93 received PBT. We con...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-017-3745-z
更新日期:2017-11-14 00:00:00
abstract:BACKGROUND:Prostate tumours are commonly poorly oxygenated which is associated with tumour progression and development of resistance to chemotherapeutic drugs and radiotherapy. Fibroblast growth factor 8b (FGF8b) is a mitogenic and angiogenic factor, which is expressed at an increased level in human prostate tumours an...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-10-596
更新日期:2010-10-30 00:00:00
abstract:BACKGROUND:Malignant glioma is a common primary tumor of the central nervous system. Brevican, an abundant extracellular matrix component in the adult brain, plays a critical role in the process of glioma. The mechanisms for the highly invasive behavior of gliomas are still poorly understood. The aim of this study was ...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-12-607
更新日期:2012-12-19 00:00:00
abstract:BACKGROUND:Over-expression of HER2 in a subset of breast cancers (HER2+) is associated with high histological grade and aggressive clinical course. Despite these distinctive features, the differences in response of HER2+ patients to both adjuvant cytotoxic chemotherapy and targeted therapy (e.g. trastuzumab) suggests t...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-10-108
更新日期:2010-03-22 00:00:00
abstract:BACKGROUND:The neural transcription factor SOX11 has been described as a prognostic marker in epithelial ovarian cancers (EOC), however its role in individual histological subtypes and tumour grade requires further clarification. Furthermore, methylation-dependent silencing of SOX11 has been reported for B cell lymphom...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-11-405
更新日期:2011-09-24 00:00:00
abstract:BACKGROUND:The use of combined positron emission tomography/computerised tomography (PET/CT) scanners in oncology has been shown to improve the staging of tumours and the detection of relapses. However, mis-registration errors are increasingly recognised to be a common pitfall of PET/CT studies. CASE PRESENTATION:We r...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-7-147
更新日期:2007-08-03 00:00:00
abstract:BACKGROUND:Tamoxifen has dramatically reduced the recurrence and mortality rate of estrogen receptor positive breast cancer. However, the efficacy of tamoxifen varies between individuals and 40% of patients will have a recurrence despite adjuvant tamoxifen treatment. Factors that predict tamoxifen efficacy would be hel...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-13-612
更新日期:2013-12-28 00:00:00
abstract:BACKGROUND:Combinations of trastuzumab with either docetaxel or vinorelbine are considered valuable treatment options for HER2-positive metastatic breast cancer patients. We performed a retrospective comparison of the clinical outcomes associated with either one of these combinations. METHODS:From a multi-institutiona...
journal_title:BMC cancer
pub_type: 杂志文章,多中心研究
doi:10.1186/1471-2407-10-28
更新日期:2010-02-01 00:00:00
abstract:BACKGROUND:Whether chronic inflammation increases prostate cancer risk remains unclear. This study investigated whether chronic inflammatory diseases (CID) or anti-inflammatory medication use (AIM) were associated with prostate cancer risk. METHODS:Fifty-five thousand nine hundred thirty-seven cases (all prostate canc...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-019-5846-3
更新日期:2019-06-21 00:00:00
abstract:BACKGROUND:Regulatory T cells (Tregs), particularly the CD4(+)CD25(+)Foxp3(+) Tregs, down regulate immunity and promote tumor cell growth by directly suppressing CD8(+) and CD4(+) T cells. Alternatively they can promote tumor growth by generating interleukin-10 (IL-10) and transforming growth factor β (TGFβ) in situ, w...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-016-2473-0
更新日期:2016-07-07 00:00:00
abstract:BACKGROUND:Patients undergoing surgery for esophageal cancer have a high risk for postoperative deterioration of lung function and pulmonary complications. This is partly due to one-lung ventilation during thoracotomy. This often accounts for prolonged stay on intensive care units, delayed postoperative reconvalescence...
journal_title:BMC cancer
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1186/s12885-017-3400-8
更新日期:2017-06-14 00:00:00
abstract:BACKGROUND:Exercise has favorable effects on biomarkers associated with a lower risk of breast cancer, however it is unclear if higher doses of exercise provide additional effects. No clinical trial has systematically examined how different exercise volumes influence the mechanisms underlying breast cancer etiology. Th...
journal_title:BMC cancer
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1186/1471-2407-14-919
更新日期:2014-12-06 00:00:00
abstract:BACKGROUND:Prostate cancer is the single most prevalent cancer in US men whose gold standard of diagnosis is histologic assessment of biopsies. Manual assessment of stained tissue of all biopsies limits speed and accuracy in clinical practice and research of prostate cancer diagnosis. We sought to develop a fully-autom...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-11-62
更新日期:2011-02-09 00:00:00
abstract:BACKGROUND:The epithelial cell adhesion molecule (EpCAM) has been shown to be overexpressed in breast cancer and stem cells and has emerged as an attractive target for immunotherapy of breast cancer patients. This study analyzes the effects of EpCAM on breast cancer cell lines with epithelial or mesenchymal phenotype. ...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-12-501
更新日期:2012-10-30 00:00:00