Adult fast myosin pattern and Ca2+-induced slow myosin pattern in primary skeletal muscle culture.

Abstract:

:A primary muscle cell culture derived from newborn rabbit muscle and growing on microcarriers in suspension was established. When cultured for several weeks, the myotubes in this model develop the completely adult pattern of fast myosin light and heavy chains. When Ca2+ ionophore is added to the culture medium on day 11, raising intracellular [Ca2+] about 10-fold, the myotubes develop to exhibit properties of an adult slow muscle by day 30, expressing slow myosin light as well as heavy chains, elevated citrate synthase, and reduced lactate dehydrogenase. The remarkable plasticity of these myotubes becomes apparent, when 8 days after withdrawal of the ionophore a marked slow-to-fast transition, as judged from the expression of isomyosins and metabolic enzymes, occurs.

authors

Kubis HP,Haller EA,Wetzel P,Gros G

doi

10.1073/pnas.94.8.4205

subject

Has Abstract

pub_date

1997-04-15 00:00:00

pages

4205-10

issue

8

eissn

0027-8424

issn

1091-6490

journal_volume

94

pub_type

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