Abstract:
:A primary muscle cell culture derived from newborn rabbit muscle and growing on microcarriers in suspension was established. When cultured for several weeks, the myotubes in this model develop the completely adult pattern of fast myosin light and heavy chains. When Ca2+ ionophore is added to the culture medium on day 11, raising intracellular [Ca2+] about 10-fold, the myotubes develop to exhibit properties of an adult slow muscle by day 30, expressing slow myosin light as well as heavy chains, elevated citrate synthase, and reduced lactate dehydrogenase. The remarkable plasticity of these myotubes becomes apparent, when 8 days after withdrawal of the ionophore a marked slow-to-fast transition, as judged from the expression of isomyosins and metabolic enzymes, occurs.
journal_name
Proc Natl Acad Sci U S Aauthors
Kubis HP,Haller EA,Wetzel P,Gros Gdoi
10.1073/pnas.94.8.4205subject
Has Abstractpub_date
1997-04-15 00:00:00pages
4205-10issue
8eissn
0027-8424issn
1091-6490journal_volume
94pub_type
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