Role of intracellular calcium stores in contrast medium-induced renal vasoconstriction.

Abstract:

RATIONALE AND OBJECTIVES:Renovascular smooth muscle contractility, an important factor in contrast media-induced nephrotoxicity, depends on intracellular Ca2+ concentration, which is composed of extracellular Ca2+ influx and intracellular Ca2+ release. These factors were investigated in contrast media-induced renal vasoconstriction in an in vitro model. METHODS:KCl-induced isometric contractions of rabbit renal artery were compared with contractions elicited by contrast media (diatrizoate, iohexol, iopamidol). Measurements were made after incubation with the Ca2+ channel blockers nifedipine, verapamil, and diltiazem to assess the role of extracellular Ca2+ influx and after ryanodine and thapsigargin to investigate the role of intracellular Ca2+ release. RESULTS:The Ca2+ channel blockers partially inhibited contractions induced by contrast media, while KCl-induced contractions were completely abolished. Ryanodine and thapsigargin also markedly inhibited contrast media-induced contractions. CONCLUSION:Ionic and nonionic contrast media induced quantitatively different renal vasocontractions. Ca2+ channel blockers inhibited this vasocontraction only slightly compared with intracellular Ca2+ release blockers.

journal_name

Acad Radiol

journal_title

Academic radiology

authors

Drescher P,Rauch D,Madsen PO

doi

10.1016/s1076-6332(96)80298-4

subject

Has Abstract

pub_date

1996-11-01 00:00:00

pages

912-8

issue

11

eissn

1076-6332

issn

1878-4046

journal_volume

3

pub_type

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