Abstract:
:The effect of culture medium on glutathione (GSH) dependent detoxification defence system of primary cultured hepatocyte from either male or female rats was studied. Intracellular reduced (GSH) and oxidized glutathione (GSSG), and six GSH-related enzyme activities, including GSH peroxidase (GSH Px), GSH reductase (GSH Rd), cytosolic GSH S-transferase (cGST), microsomal GSH S-transferase (mGST), gamma-glutamyl transpeptidase (GTP), and gamma-glutamylcysteine synthetase (GCS), were investigated during a 6-day culture. Media free of fetal bovine serum (FBS) and with 2.5 or 10% FBS were used. Whatever the medium, there was an initial decrease of intracellular GSH and GSSG, a threefold increase of GSH at day 3 and fourfold increase of GSSG at day 4, later decreasing to their original level at day 6. The activities of all six GSH-related enzymes of male and female hepatocytes remained relatively stable during the first 72h, then gradually decreased to 50-80% of initial activities. With the exception of cGST, time-course profiles of other enzyme activities were not significantly different among various media. In both sexes, higher cGST activity was maintained for cells cultured in the presence of FBS. Results of immunoblotting analysis of cytosolic GST isozymes indicate that the placental form of GST (Yp) was markedly increased after plating and the extent of increase of Yp was higher in the presence of FBS. Despite the culture medium, the level of GST isoform Ya was maintained steadily for 6 days, however, Yb was maintained during the first 3 days and then decreased. In terms of the gender difference, GSH Px and GTP activities of hepatocytes from females were significantly greater than of males over the entire culture period. Results indicate that FBS seems not to be absolutely essential in maintaining GSH level and most of the GSH-related enzyme activities in rat hepatocytes. Furthermore, GSH levels and GSH-related enzyme activities of hepatocytes from female rats were similar to those from male rats.
journal_name
Arch Toxicoljournal_title
Archives of toxicologyauthors
Lii CK,Wang ST,Chen HW,Sheen LYdoi
10.1007/s002040050345subject
Has Abstractpub_date
1996-01-01 00:00:00pages
822-9issue
12eissn
0340-5761issn
1432-0738journal_volume
70pub_type
杂志文章abstract::We investigated the influence of the dopamine antagonist haloperidol on the neurotransmitter content in the developing rat brain. Dopamine (DA), serotonin (5-HT) and the corresponding metabolites dopac, HVA and HIAA were determined in the corpus striatum of the rat between day 1 and day 21 pn by HPLC with electro-chem...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/BF01973388
更新日期:1992-01-01 00:00:00
abstract::We investigated the hepatotoxicity induced by AQ using a glutathione (GSH)-depleted mice model. Although sole administration of either AQ or L-buthionine-S,R-sulfoxinine (BSO), a well-known GSH synthesis inhibitor, produced no significant hepatotoxicity, combined administration of AQ with BSO induced hepatotoxicity ch...
journal_title:Archives of toxicology
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journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/s002040050499
更新日期:1998-04-01 00:00:00
abstract::The hepatotoxicity of acetaminophen (APAP) overdose depends on metabolic activation to a toxic reactive metabolite via hepatic mixed function oxidase. In vitro studies have indicated that APAP may also be cooxidized by prostaglandin H synthetase. The present experiments were designed to assess the possible contributio...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/BF01972990
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abstract::Quantitative structure-activity relationships (QSAR) have suggested the importance of hydrogen bonding in relation to activation of the sensory irritant receptor by nonreactive volatile organic chemicals. To investigate this possibility further, three model compounds with different hydrogen bond acidity, trifluoroetha...
journal_title:Archives of toxicology
pub_type: 杂志文章
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journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/BF00332390
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abstract::Early hepatocyte death occurs in most liver injury cases and triggers liver inflammation, which in combination with other risk factors leads to the development of liver disease. However, the pathogenesis of early phase hepatocyte death remains poorly understood. Here, C57BL/6J mice were treated with the hepatotoxic dr...
journal_title:Archives of toxicology
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journal_title:Archives of toxicology
pub_type: 杂志文章,评审
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journal_title:Archives of toxicology
pub_type: 杂志文章
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journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/s00204-014-1197-7
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journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/s00204-011-0788-9
更新日期:2012-04-01 00:00:00
abstract::The EU-EuroMix project adopted the strategy of the European Food Safety Authority (EFSA) for cumulative risk assessment, which limits the number of chemicals to consider in a mixture to those that induce a specific toxicological phenotype. These so-called cumulative assessment groups (CAGs) are refined at several leve...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/s00204-018-2320-y
更新日期:2018-12-01 00:00:00
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journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/BF00353353
更新日期:1976-12-23 00:00:00
abstract::Garenoxacin is a des-F(6)-quinolone with a broad antibacterial spectrum, which has previously been shown to exhibit low chondrotoxicity in juvenile dogs compared with several other quinolones. A study was performed to determine whether the low chondrotoxicity observed in immature rats following garenoxacin treatment c...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/s00204-003-0514-3
更新日期:2004-02-01 00:00:00
abstract::In female rats, the lethality and hepatotoxicity of cerous chloride (CeCl3) were significantly altered by pretreatment with steroidal and nonsteroidal compounds (pregnenolone-16alpha-carbonitrile "PCN", dexamethasone, spironolactone, phenobarbital) that stimulate hepatic drug-metabolizine enzyme activity and by estrad...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/BF00372765
更新日期:1976-03-11 00:00:00
abstract::The present experiments were performed to test the possibility of interrupting the enterohepatic circulation of arsenic (As). Therefore the efficacy of adsorbents to bind As and/or As-DMPS adducts in vitro and their effect on the excretion of As into the feces and urine in vivo were investigated after injection of As2...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/s002040050237
更新日期:1995-01-01 00:00:00
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journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/BF00361248
更新日期:1980-11-01 00:00:00
abstract::Environmental tobacco smoke (ETS) is associated with high morbidity and mortality, mainly in children. However, few studies focus on the brain development effects of ETS exposure. Myelination mainly occurs in the early years of life in humans and the first three postnatal weeks in rodents and is sensitive to xenobioti...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/s00204-014-1343-2
更新日期:2015-11-01 00:00:00
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journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/s002040050662
更新日期:2000-04-01 00:00:00
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journal_title:Archives of toxicology
pub_type: 杂志文章
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journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/BF00293567
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journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/s00204-005-0022-8
更新日期:2006-03-01 00:00:00
abstract::Bisphenol A (BPA) is a chemical in widespread use that is under scientific discussion due to its endocrine activity. Controversies exist about how to interpret reportedly high blood concentrations measured in uncontrolled situations. Physiologically based pharmaco-/toxicokinetic modelling resulted in 10-100-fold lower...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/s00204-013-1022-8
更新日期:2013-07-01 00:00:00
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journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/s002040000196
更新日期:2001-02-01 00:00:00
abstract::3H-soman (specific activity 10 Ci/mMol), a potent irreversible cholinesterase inhibitor, was administered IV to mice in a dose of one LD-50, which corresponds to 0.25 mCi/mouse. Animals were sacrificed at 5 min, 2 h and 24 h, and whole body autoradiography was performed. High levels of radioactivity in lung and skin w...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/BF00292616
更新日期:1985-10-01 00:00:00
abstract::Experimental and/or epidemiological studies suggest that prenatal exposure to bisphenol A (BPA) may delay fetal lung development and maturation and increase the susceptibility to childhood respiratory disease. However, the underlying mechanisms remain to be elucidated. In our previous study with cultured human fetal l...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/s00204-017-2150-3
更新日期:2018-04-01 00:00:00
abstract::MicroRNAs (miRNAs) are a family of endogenous, small, noncoding RNA molecules that involved in a wide range of biological processes including differentiation, proliferation, and apoptosis. A polymorphism G>C (rs2910164) is located in the stem region opposite to the mature miR-146a sequence. In our study, we investigat...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/s00204-012-1004-2
更新日期:2013-05-01 00:00:00
abstract::The excretion and biotransformation of [14C] 1,1-dichloroethylene (vinylidene chloride, VDC) after administration of a single oral dose has been investigated in female rats. Seventy-two hours after a dose of 0.5, 5.0, and 50.0 mg/kg, 1.26, 9.70, 16.47%, respectively, are exhaled as unchanged VDC, and 13.64, 11.35, 6.1...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/BF00353707
更新日期:1979-07-11 00:00:00
abstract::Previously we reported that exposure of mouse and human mast cells to aryl hydrocarbon receptor (AhR) ligands resulted in reactive oxygen species (ROS)- and calcium (Ca2+)-dependent activation of mast cells in vitro and in vivo. However, the mechanisms through which the AhR-ligand axis mediates stress response, Ca2+ s...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/s00204-016-1861-1
更新日期:2017-04-01 00:00:00
abstract::Previous studies have shown the antidotal efficacy of topical iodine at 15 and 30 min post-exposure to sulfur mustard (SM). Here we demonstrate efficacy at longer intervals (20, 30, 45, and 60 min, respectively, for data) using an improved topical povidone-iodine preparation termed N66, which contains steroidal and no...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/s00204-003-0523-2
更新日期:2004-03-01 00:00:00