Abstract:
:T cell receptor (TCR) transgenic mice have been used extensively to study T cell development in vivo. Such studies have demonstrated high levels of expression of the TCR transgenes. Although a number of human T cell receptors appear to play a role in the development of autoimmune diseases, in vitro studies have proven inadequate for investigation of their putative pathogenicity. Several groups have reported the isolation of myelin basic protein (MBP)-reactive T cell clones from patients with multiple sclerosis and many of the T cell receptors from such clones have been well characterized. Since a number of inbred mouse strains have demonstrated susceptibility to a similar T cell-mediated inflammatory demyelinating disease known as EAE, a useful animal model is likely to be generated by expressing human MBP-specific TCR in susceptible mice. As a first step toward this goal we have cloned a number of TCR genes into an expression vector previously used for murine TCR genes. Here we report the development of a rapid cloning system for the generation of mouse-human chimeric TCR transgene constructs and the use of this system for the production of MBP-specific TCR transgenes. Human MBP-specific TCR transgenic mice will provide a unique system for the investigation of T cell-mediated demyelinating disease in the central nervous system (CNS).
journal_name
J Neurosci Resjournal_title
Journal of neuroscience researchauthors
Baker AM,Wang Y,Richert JRdoi
10.1002/(SICI)1097-4547(19960815)45:4<487::AID-JNRsubject
Has Abstractpub_date
1996-08-15 00:00:00pages
487-91issue
4eissn
0360-4012issn
1097-4547pii
10.1002/(SICI)1097-4547(19960815)45:4<487::AID-JNRjournal_volume
45pub_type
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