Abstract:
OBJECTIVE:To understand whether very low density lipoprotein (VLDL) and oxidatively modified very low density lipoprotein (OX-VLDL) can stimulate vascular smooth muscle cells (SMCs) to express high level of monocyte chemotactic protein (MCP-1) mRNA. METHODS:LDL and VLDL were isolated from normal blood donors by density gradient ultracentrifugation and oxidatively modified by adding CuCl2 and incubation at 37 degrees C for 24 hours. Rabbit aortic SMCs were cultured by a substrate-attached explant method, and their total RNA was extracted by guanidinium isothiocyanate method. The expression of MCP-1 mRNA in SMCs was examined by dot blotting analysis using a probe of gamma-22P-end-labeled 35-mer oligonucleotide. RESULTS:Dot blotting analysis showed that cultured SMCs can express MCP-1 mRNA and the level of MCP-1 mRNA was increased significantly in SMCs after 24-hour coculture with VLDL (6-fold) and OX-VLDL (20-fold). CONCLUSIONS:VLDL and OX-VLDL both can induce MCP-1 mRNA expression in cultured rabbit aortic SMCs and the latter is more effective than the former.
journal_name
Chin Med J (Engl)journal_title
Chinese medical journalauthors
Ruan Q,Deng Z,Song Jsubject
Has Abstractpub_date
1996-03-01 00:00:00pages
206-9issue
3eissn
0366-6999issn
2542-5641journal_volume
109pub_type
杂志文章abstract:OBJECTIVE:To explore the effects of ultrafiltration technique in preventing and relieving pulmonary injury in children undergoing open heart surgery and cardiopulmonary bypass (CPB). METHODS:Thirty cases with congenital heart defects were divided into a control group and an experimental group. In the control group, co...
journal_title:Chinese medical journal
pub_type: 临床试验,杂志文章
doi:
更新日期:2003-10-01 00:00:00
abstract:OBJECTIVE:To investigate the possibility of using melanoma antigen-1 (MAGE-1) peptide as a tumor vaccine to treat hepatocellular carcinoma (HCC). METHODS:The expressions of MAGE-1 in 8 HCC cell lines and in liver cancer tissue from a patient were detected using RT-PCR. The type of human leucocyte antigen I(HLA I) of b...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2002-07-01 00:00:00
abstract:BACKGROUND:It is unclear whether a history of paroxysmal atrial fibrillation (PAF) would impact the effect of catheter ablation on persistent atrial fibrillation (AF). This study aimed to compare the effect of catheter ablation on persistent AF with and without a history of PAF. METHODS:One hundred and eighty-three pa...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2012-03-01 00:00:00
abstract::An epidemic of parenterally transmitted non-A, non-B hepatitis (PT-NANBH) occurred in plasmapheresis donors in Guan County, Hebei Province, China, in 1985. PT-NANBH was diagnosed by epidemiological studies and serological exclusion of HAV, HBV, CMV and EBV infections. Recently, 163 sera samples of 108 patients with PT...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:1991-06-01 00:00:00
abstract:OBJECTIVE:The objective was to provide a brief history of J wave syndromes and to summarize our current understanding of their molecular, ionic, cellular mechanisms, and clinical features. We will also discuss the existing debates and further direction in basic and clinical research for J wave syndromes. DATA SOURCES:...
journal_title:Chinese medical journal
pub_type: 杂志文章,评审
doi:10.4103/0366-6999.154320
更新日期:2015-04-05 00:00:00
abstract:BACKGROUND:Esophageal carcinoma is a common kind of malignant tumor and about 90% of which is esophageal squamous cell carcinoma (ESCC), and it has a high incidence in China. In recent years it has been argued that angiogenesis plays a key role in tumor growth and metastasis and it is a complex process influenced by ma...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2008-05-20 00:00:00
abstract:OBJECTIVE:To investigate whether PreS1 antigen and/or anti-PreS1 antibody in serum might have some significance different from routine hepatitis B virus (HBV) markers in hepatitis B virus infection. METHODS:Forty-six acute hepatitis B patients and 377 chronic persistent hepatitis patients were examined to assess the c...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:1999-04-01 00:00:00
abstract:BACKGROUND:The CYP2C19 G681A single polymorphism has been proven to affect clopidogrel responsiveness. However, the effect of coexisting polymorphisms of other genes has not yet been reported in the Chinese population. This study investigated the effect of coexisting polymorphisms of CYP2C19 and P2Y12 on clopidogrel re...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2013-03-01 00:00:00
abstract:BACKGROUND:Effects of icariin on airway inflammation in asthmatic rats and the intervention of LPS induced inflammation are interfered with the machanism of icariin. Our study aimed to observe the effect of icariin on ovalbumin-induced imbalance of Th1/Th2 cytokine expression and its mechanism. METHODS:Sixty male SD r...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2011-09-01 00:00:00
abstract:BACKGROUND:Gender is one of the risk factors accounting for the high prevalence of adolescent myopia. Considerable research results have shown that myopia incidence of female is higher than that of male. This study aimed to analyze the correlation between ocular parameters and serum estrogen level and to investigate th...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:10.4103/0366-6999.151669
更新日期:2015-03-05 00:00:00
abstract:OBJECTIVE:To investigate CTLA4-Ig's potential role in therapy for allergic asthma by blocking B7/CD28 interactions with cytotoxic T lymphocyte antigen 4-Ig (CTLA4-Ig). METHODS:We divided BALB/C mice into the three groups: Sham/Sham (control), ovalbumin (OVA)/OVA and mCTLA4-Ig. Blood, bronchoalveolar lavage, histology ...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2003-03-01 00:00:00
abstract:BACKGROUND:It is a surgical dilemma when patients present with both severe heart disease and neoplasms. The best surgical treatment remains controversial. This study aimed to analyze the early and long-term results of simultaneous surgical treatment of severe heart disease and neoplasms. METHODS:We reviewed the clinic...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2011-07-05 00:00:00
abstract:BACKGROUND:Chimerism analysis is an important tool for the surveillance of post-transplant engraftment. It offers the possibility of identifying impending graft rejection and recurrence of underlying malignant or non-malignant disease. Here we investigated the quantitative chimerism kinetics of 21 relapsed leukemia pat...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2012-06-01 00:00:00
abstract:BACKGROUND:Genetic factors can influence antihypertensive response to metoprolol, and many studies focused on the relationship between the genotype in beta1-adrenergic receptor and blood pressure (BP), little was known about the association of angiotensin-converting enzyme (ACE) genotype with the therapeutic result of ...
journal_title:Chinese medical journal
pub_type: 临床试验,杂志文章
doi:
更新日期:2010-06-01 00:00:00
abstract:OBJECTIVE:To investigate the complications of laparoscopic cholecystectomy in China. METHODS:All Chinese articles about laparoscopic cholecystectomy published between April 1994 and November 1995 were identified through CMCC (Chinese Medical Computerized Contents). From more than 600 titles, 105 articles were screened...
journal_title:Chinese medical journal
pub_type: 杂志文章,评审
doi:
更新日期:1997-09-01 00:00:00
abstract:BACKGROUND:Transforming growth factor-beta1 (TGF-beta1) exerts strong fibrogenic potential in culture-activated HSCs. Smad4 is a key intracellular mediator for the transforming growth factor-beta (TGF-beta) superfamily of growth factors. The aim of this study was to assess the effects of the antisense Smad4 gene on Ito...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2004-08-01 00:00:00
abstract:OBJECTIVE:To determine the distribution of aortic arch and intra/extracranial cerebral arterial atherosclerosis in Chinese patients who had suffered acute ischemic strokes. METHODS:Eighty-nine patients with acute ischemic strokes were included in this study. Transesophageal echocardiography (TEE) was used to evaluate ...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2003-12-01 00:00:00
abstract:BACKGROUND:The prognostic values of the coronary computed tomography angiography (CCTA) score for predicting future cardiovascular events have been previously demonstrated in numerous studies. However, few studies have used the rich information available from CCTA to detect functionally significant coronary lesions. We...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:10.4103/0366-6999.194636
更新日期:2016-12-05 00:00:00
abstract:BACKGROUND:Spinal cord injury (SCI) is a worldwide medical concern. This study aimed to elucidate the mechanism underlying the protective effect of hyperbaric oxygen (HBO) against SCI-induced neurologic defects in rats via exploring the stromal cell-derived factor-1 (SDF-1)/CXC chemokine receptor 4 (CXCR4) axis and exp...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:10.1097/CM9.0000000000000115
更新日期:2019-03-20 00:00:00
abstract::Filariasis control including a preparatory phase in the early 50s and two more extensive campaigns in the late 50s and 70s, has gained remarkable achievements. By the end of 1987, filariasis in 781 (90.4%) of 864 counties/cities was brought under control, i.e., the microfilariasis rate declined to less than 1% in the ...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:1991-06-01 00:00:00
abstract:OBJECTIVE:To determine whether mutation of the coding or junction region of glucokinase gene (GCK) is also the pathogenic cause of the common form of non-insulin-dependent diabetes mellitus (NIDDM) in Chinese. METHODS:Single strand conformation polymorphism (SSCP) analysis was performed after the 12 exons and junction...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:1996-11-01 00:00:00
abstract:BACKGROUND:Although there were several clinical and experimental studies discussing the pathogenesis of dural arteriovenous fistula (DAVF), the pathological process leading to intracranial DAVF so far remains unknown. In this study, we investigated the expression of vascular growth factors in order to elucidate the pos...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2004-12-01 00:00:00
abstract:BACKGROUND:Mitochondrial DNA 4977-bp deletion (DeltamtDNA(4977)) was reported in many human neoplasia. However, its biological significance remains to be evaluated and the molecular mechanism needs to be investigated. In this study, we analyzed the frequency of DeltamtDNA(4977) in gastric cancer (GC) cell lines and tis...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2009-02-20 00:00:00
abstract:BACKGROUND:Pleomorphic hyalinizing angiectatic tumor (PHAT) of soft parts is a rare soft tissue tumor, which is generally considered low-grade. To distinguish the tumor from other soft tissue lesions, we analyzed the clinicopathologic and ultrastructural features, immunophenotypes, and flow cytometric DNA ploidy of PHA...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2007-05-20 00:00:00
abstract:BACKGROUND:Intravascular ultrasound (IVUS) examination can provide useful information during endovascular stent graft repair. However, its actual clinical utility in thoracic endovascular aortic repair (TEVAR) for type B aortic dissection (type B-AD) remains unclear, especially in complicated aortic dissection. We eval...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:10.4103/0366-6999.163386
更新日期:2015-09-05 00:00:00
abstract:BACKGROUND:Chronic intermittent hypoxia (CIH) is the most important pathophysiologic feature of sleep apnea syndrome (SAS). To explore the relationship between SAS and dementia, the effects of CIH on the expression of Nip3, neuron apoptosis and beta-amyloid protein deposit in the brain cortex of the frontal lobe of mic...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2009-01-05 00:00:00
abstract:BACKGROUND:It is important to modulate the expression of glucocorticoids receptor (GR) in tress and maintain the immunity homeostasis in sepsis process. Rhubarb have been shown to have potential effects on anti-inflammatory and immune modulation. The present study was designed to investigate the effects of rhubarb on t...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:10.1097/CM9.0000000000000201
更新日期:2019-05-20 00:00:00
abstract:OBJECTIVE:To investigate the effects of gypenosides (Gs) on cellular immunocompetence in the gamma-ray-irradiated mice. MATERIALS AND METHODS:Tested mice of ICR strain were treated continuously with Gs for 10 days before or after 4 Gy gamma-irradiation. Body weight and splenic weight of mice were measured. The blastog...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:1996-02-01 00:00:00
abstract::Atriopeptin III (AP III) and its six analogues were synthesized by solid phase method and their diuretic and hypotensive activities were determined. Among these analogues, analogue [D-Ala-5, D-Arg-23] AP III was nearly 10 times as potent as AP III in diuretic activity while its hypotensive activity increased only 50% ...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:1995-04-01 00:00:00
abstract:BACKGROUND:As a Chinese Traditional Medicine product, Kuntai capsule could improve the peri-menopausal symptoms in postmenopausal women. But it is still not clear whether Kuntai capsule has a good effect on alleviating peri-menopausal symptoms induced by gonadotropin releasing hormone agonist (GnRH-a) treatment. The pu...
journal_title:Chinese medical journal
pub_type: 杂志文章,随机对照试验
doi:10.4103/0366-6999.151057
更新日期:2015-02-20 00:00:00