Abstract:
:The expression of the preproglucagon gene in vertebrates is markedly species- and tissue-dependent. Three peptides derived from the posttranslational processing of preproglucagon were isolated from an extract of the pancreas of the urodele Amphiuma tridactylum (threetoed amphiuma). The primary structures of the peptides indicated identity with glucagon (HSQGTFTSDY10 SKYLDNRRAQ20 DFIQWLMST), glucagon-like peptide-1 (GLP-1) (HADGTLTSDI10 SSFLEKQATK20 EFIAWLVSGR30 GRRQ), and glucagon-like peptide-2 (GLP-2) (HADGSFTSDI10 NKVLDTIAAK20 EFLNWLISTK30 VTE). Thus, in a urodele, as in the bullfrog but in contrast to the chicken and all nontetrapod species yet studied, pancreatic preproglucagon mRNA encodes a GLP-2 sequence. The amino acid sequence of glucagon has been better conserved during evolution of tetrapods (3 substitutions between amphiuma and human) than the sequences of either GLP-1 (7 substitutions) or GLP-2 (15 substitutions). Pancreatic polypeptide was also isolated from the extract and its primary structure (APKEPEHPGD10 DASPEQLEKY20 YQDLFQYIIF30 ITRPRY.NH2) indicates that the amino acid sequence of this peptide has been very poorly conserved, even among the amphibia. Amphiuma pancreatic somatostatin is identical to mammalian somatostatin-14.
journal_name
Gen Comp Endocrinoljournal_title
General and comparative endocrinologyauthors
Cavanaugh ES,Nielsen PF,Conlon JMdoi
10.1006/gcen.1996.0003subject
Has Abstractpub_date
1996-01-01 00:00:00pages
12-20issue
1eissn
0016-6480issn
1095-6840pii
S0016-6480(96)90003-9journal_volume
101pub_type
杂志文章abstract::Progesterone, 4-androstenedione, testosterone, dihydrotestosterone, 5 alpha-androstane-3 alpha,17 beta-diol (3 alpha-diol), 5 alpha-androstane-3 beta, 17 beta-diol (3 beta-diol), estrone, and estradiol levels were determined by radioimmunoassay in the different lobules of the testis of Salamandra salamandra throughout...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(85)90337-5
更新日期:1985-05-01 00:00:00
abstract::Molecular cloning of thyrotropin-releasing hormone receptors (TRHR) was performed in a teleost, the sockeye salmon (Oncorhynchus nerka). Four different TRHR cDNAs were cloned and named TRHR1, TRHR2a, TRHR2b and TRHR3 based on their similarity to known TRHR subtypes in vertebrates. Important residues for TRH binding we...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2011.07.011
更新日期:2011-11-01 00:00:00
abstract::Potamopyrgus antipodarum is a promising test organism that is often used in ecotoxicology, both in laboratory and field exposures. As no data are available on the physiological variation range of its life-traits and the biomarkers it uses, we studied the variation of fecundity, steroid levels and energy reserves over ...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2011.03.011
更新日期:2011-06-01 00:00:00
abstract::Growth hormone (GH) and insulin-like growth factor-I (IGF-I) play major roles in the endocrine regulation of fish growth, but their interdependency and mode of action has not been well elucidated. The GH-IGF-I system is essential for normal vertebral growth in mouse, but this has not been studied in fish. To study the...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2004.12.005
更新日期:2005-05-15 00:00:00
abstract::Triorganotins, such as tributyltin chloride (TBTCl), are environmental contaminants that are commonly found in the antifouling paints used in ships and other vessels. The importance of TBTCl as an endocrine-disrupting chemical (EDC) in different animal models is well known; however, its adverse effects on the thyroid ...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2014.07.027
更新日期:2014-09-15 00:00:00
abstract::Gonadotropin (GTH) secretion is known to be under inhibitory dopaminergic control in several species of fish. To investigate whether this is also the case in the Atlantic croaker (Micropogonias undulatus), juvenile and adult croaker were treated with a gonadotropin-releasing hormone analog (des-Gly10D-Ala6Pro9 n ethyl...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/s0016-6480(89)80045-0
更新日期:1989-06-01 00:00:00
abstract::Three species of fish that are phylogenetically older than other members of the bony fish lineage were selected to determine if gonadotropin-releasing hormone (GnRH) is present in their brains. Brain extracts were prepared from each species and found to contain immunoreactive (ir) GnRH. To further characterize the mol...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(91)90063-c
更新日期:1991-10-01 00:00:00
abstract::The widespread use of P-nitrophenol (PNP) as a raw material in pesticides, medicines and dyes has led to environmental pollution. PNP is a well-known endocrine disruptor in mammals and quails. This study investigated the effects of long-term PNP exposure on the testicular development and semen quality of roosters. Pub...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2020.113656
更新日期:2021-01-15 00:00:00
abstract::A long-unresolved question in the developmental biology of Drosophila melanogaster has been whether methyl farnesoid hormones secreted by the ring gland are necessary for larval maturation and metamorphosis. In this study, we have used RNAi techniques to inhibit 3-Hydroxy-3-Methylglutaryl CoA Reductase (HMGCR) express...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2009.07.006
更新日期:2010-01-15 00:00:00
abstract::High-affinity, limited-capacity, 3,5,3'-triiodo-L-thyronine (T3)-binding sites were established by in vitro saturation analysis in cell nuclei of the pituitary gland of arctic charr. The sites were extracted from the purified nuclei using 0.4 M NaCl and incubated with [125I]T3 in the presence of 0.2 M NaCl. T3 saturab...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(90)90202-w
更新日期:1990-01-01 00:00:00
abstract::A biochemical analysis was made of the crustacean hyperglycemic hormone (CHH) of the crayfish Astacus leptodactylus, as present in the CHH-producing perikarya, in the axonal tract and in the sinus gland, respectively. Hyperglycemic material was analyzed by polyacrylamide gel electrophoresis (PAGE and SDS-PAGE) and hig...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(86)90202-9
更新日期:1986-02-01 00:00:00
abstract::Recently, we reported the cloning of a cDNA fragment from Fundulus heteroclitus liver encoding the open reading frame of type 2 deiodinase (FhD2). We here report the cloning of 14 kb of genomic sequence from F. heteroclitus that includes the previously reported coding region of the F. heteroclitus Dio2 gene (FhDio2), ...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/s0016-6480(02)00071-0
更新日期:2002-09-01 00:00:00
abstract::Gonadotropin-releasing hormones (GnRH) constitute a family of neuropeptides which are important regulators of reproduction in vertebrates. The effect of mammalian GnRH (mGnRH), salmon GnRH, chicken GnRH-I, chicken GnRH-II, and lamprey GnRH-I on [3H]thymidine incorporation into DNA of dissociated gonadal cells of marin...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1006/gcen.1998.7186
更新日期:1999-01-01 00:00:00
abstract::Glucocorticoid hormones allow individuals to rapidly adjust their physiology and behavior to meet the challenges of a variable environment. An individual's baseline concentration of glucocorticoids can reflect shifts in life history stage and resource demands while mediating a suite of physiological and behavioral cha...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2017.07.006
更新日期:2018-01-15 00:00:00
abstract::In order to elucidate details on the development of the circadian clock, the effects of light on the in vitro melatonin (MT) release and the presence of mRNAs of several clock genes in the embryonic chicken pineal gland were investigated. Chicken embryos of various developmental stages were exposed to stimuli of light...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2007.01.008
更新日期:2007-06-01 00:00:00
abstract::Cholesterol transport across the mitochondrial membrane is rate-limiting for steroidogenesis in vertebrates. Previous studies in fish have characterized expression of the steroidogenic acute regulatory protein, however the function and regulation of other genes and proteins involved in piscine cholesterol transport ha...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2011.04.027
更新日期:2011-08-01 00:00:00
abstract::The effects of fasting and feeding on the brain expression of orexin (OX), tyrosine hydroxylase (TH), peptide Y (PY) and cholecystokinin (CCK) were examined in the blind cavefish Astyanax fasciatus mexicanus. A 10-days fasting period induced increases in both OX and TH brain mRNA expression but had no effect on PYY an...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2012.12.011
更新日期:2013-03-01 00:00:00
abstract::Three experiments were performed to determine the role of juvenile hormone (JH) in worker reproduction in queenless colonies of honey bees. In Experiment 1, egg-laying workers had low hemolymph titers of JH, as did bees engaged in brood care, while foragers had significantly higher titers. Experiment 2 confirmed these...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(92)90055-o
更新日期:1992-09-01 00:00:00
abstract::The conversion in vitro of cholesterol (Ch) to nonpolar metabolites and ecdysteroids was studied in Y-organs of the xanthid crab, Menippe mercenaria. In one set of experiments, Y-organs were prelabeled in vivo by injecting crabs with 100 microCi of [3H]Ch, and halved glands were then incubated for 24 and 48 hr in the ...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(92)90255-i
更新日期:1992-11-01 00:00:00
abstract::Gonadotropin-releasing hormone (GnRH) of vertebrates is now believed to have multiple functions in addition to its role as a hypophysiotropic hormone, as originally defined. Recently, it has been shown that GnRH occurs also in the ascidians, which are considered ancestral chordates. Here the author shows that GnRH ind...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1006/gcen.2001.7728
更新日期:2001-12-01 00:00:00
abstract::The courtship behavior of the male ring dove (Streptopelia risoria) combines aggressive displays (chasing, bowing) and nest-oriented displays (nest soliciting). Aggressive displays depend on circulating testosterone, whereas nest soliciting is estrogen-dependent and appears to depend on the aromatization of androgen i...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1006/gcen.2001.7608
更新日期:2001-04-01 00:00:00
abstract::Homogenates of ovaria as well as testes from the locust Locusta migratoria were analyzed for the presence of pregnenolone, testosterone, and estradiol by gas chromatography followed by mass spectrometry. Selected ion monitoring analyses revealed that the molecular ion and two characteristic fragment ions of pregnenolo...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(89)90034-8
更新日期:1989-10-01 00:00:00
abstract::cDNA encoding the beta type of thyroid hormone receptor (THR) was cloned from a lambda gt10 library prepared from the whole bodies of metamorphosing flounder (Paralichthys olivaceus). The deduced amino acid sequence of the flounder THR beta (fTHR beta 1) showed higher homologies to the known THR beta s of other verteb...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1006/gcen.1995.1102
更新日期:1995-08-01 00:00:00
abstract::The brains of goldfish and other teleosts contain high-affinity binding sites for [3H][3-methyl-His2]thyrotropin-releasing hormone [( 3H]MeTRH) which closely resemble TRH receptors in mammalian brain and pituitary gland in apparent dissociation constant (KD = 3-4 nM), in pharmacology for eight TRH analogs, and in exhi...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(84)90233-8
更新日期:1984-01-01 00:00:00
abstract::Using DNA-cellulose affinity chromatography, an estrogen-binding component having the properties of a classical estrogen receptor was characterized from testicular cytosol of the freshwater turtle, Chrysemys picta. This putative cytoplasmic receptor exhibited high affinity (Kd = 7.0 X 10(-10) M), low capacity (1-4 fmo...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(83)90111-9
更新日期:1983-11-01 00:00:00
abstract::Three distinct thyroid hormone receptor (TR) cDNAs (gTRalphaA, gTRalphaB and gTRbeta) were cloned and sequenced in orange-spotted grouper (Epinephelus coioides). The cDNA of gTRalphaA consisted of 1555-bp and encoded a putative protein of 416 amino acids, while the cDNA of gTRalphaB consisted of 1459-bp and encoded 39...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2008.08.015
更新日期:2008-11-01 00:00:00
abstract::The in vitro effects of salmon gonadotropin-releasing hormone (sGnRH), substance P (SP), and their antagonists on prostaglandin F2 alpha (PGF2 alpha), prostaglandin E2 (PGE2), progesterone, androgens, and estradiol-17 beta release by follicles and corpus luteum (CL) of the oviparous lizard, Podarcis sicula sicula, wer...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1006/gcen.1994.1018
更新日期:1994-02-01 00:00:00
abstract::The maternal control directing the very first hours of life is of pivotal importance for ensuring proper development to the growing embryo. Thanks to the finely regulated inheritance of maternal factors including mRNAs and proteins produced during oogenesis and stored into the mature oocyte, the embryo is sustained th...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2016.03.028
更新日期:2017-05-01 00:00:00
abstract::Adrenomedullin (AM) is a multifunctional evolutionarily highly conserved peptide. Although its genomic and amino acid (aa) sequences are known in several mammalian species and in fish, the structure of the AM gene remains unknown in intermediate phyla, including birds. Here, we report the structure and aa sequence of ...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2005.02.014
更新日期:2005-08-01 00:00:00
abstract::Sex steroid hormones have been widely detected in molluscs, and experiments have shown the importance of sex steroids in sex determination, gonadal tissue maturation and gametogenesis. Nevertheless, the signaling pathways of sex steroids in invertebrates have not yet been elucidated. In order to gain insights into the...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2006.03.016
更新日期:2006-09-15 00:00:00