TRH receptors in fish.

Abstract:

:The brains of goldfish and other teleosts contain high-affinity binding sites for [3H][3-methyl-His2]thyrotropin-releasing hormone [( 3H]MeTRH) which closely resemble TRH receptors in mammalian brain and pituitary gland in apparent dissociation constant (KD = 3-4 nM), in pharmacology for eight TRH analogs, and in exhibiting marked regional differences in the density of binding sites, with highest binding in the cerebrum and lowest in the cerebellum. Fish brain differs from mammalian brain in containing a prominent additional class of much lower affinity [3H]MeTRH binding sites (KD about 15 microM), of unknown nature, which also exhibit regional differences. Fish pituitary glands contain high-affinity [3H]MeTRH binding sites, but insufficient tissue has prevented full characterization. Little or no saturable binding of [3H]MeTRH was detected in several goldfish peripheral tissues.

journal_name

Gen Comp Endocrinol

authors

Burt DR,Ajah MA

doi

10.1016/0016-6480(84)90233-8

subject

Has Abstract

pub_date

1984-01-01 00:00:00

pages

135-42

issue

1

eissn

0016-6480

issn

1095-6840

pii

0016-6480(84)90233-8

journal_volume

53

pub_type

杂志文章