Abstract:
:High-affinity, limited-capacity, 3,5,3'-triiodo-L-thyronine (T3)-binding sites were established by in vitro saturation analysis in cell nuclei of the pituitary gland of arctic charr. The sites were extracted from the purified nuclei using 0.4 M NaCl and incubated with [125I]T3 in the presence of 0.2 M NaCl. T3 saturable binding attained equilibrium after 18-24 hr of incubation at 4 degrees. The association constant ranged from 6.7 to 20.1 liters.mol-1 x 10(9), indicating a T3 affinity greater than that for T3-binding sites in rainbow trout liver. The maximal binding capacity ranged from 0.93 to 2.05 10(-13) mol.mg DNA-1, representing a mean site abundance corresponding to 60% of that for nuclei from trout liver. Thyroxine (T4) completely displaced [125I]T3 in the pituitary nuclei of arctic charr and T3 completely displaced [125I]T4 in the pituitary nuclei of rainbow trout, suggesting that in salmonids both T4 and T3 bind to the same single class of sites. However, the site affinity for T4 was approximately 20-50x less than that for T3. The possible roles of these sites in pituitary function as well as their relationship to other nuclear T3-binding sites in salmonid fish are discussed.
journal_name
Gen Comp Endocrinoljournal_title
General and comparative endocrinologyauthors
Bres O,Eales JGdoi
10.1016/0016-6480(90)90202-wsubject
Has Abstractpub_date
1990-01-01 00:00:00pages
23-8issue
1eissn
0016-6480issn
1095-6840journal_volume
77pub_type
杂志文章abstract::A clone encoding thyroid hormone receptor-beta (TR-beta) was isolated from a sea bream (Sparus aurata) ovary cDNA library. Sea bream (sb)TR-beta is closely related to its counterparts from other vertebrates and, like them, preferentially binds T3 rather than T4. However, the putative sbTR-beta protein contains a nine-...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1006/gcen.2001.7649
更新日期:2001-07-01 00:00:00
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journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1006/gcen.2001.7621
更新日期:2001-05-01 00:00:00
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pub_type: 杂志文章
doi:10.1006/gcen.1993.1170
更新日期:1993-12-01 00:00:00
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journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1006/gcen.1999.7311
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abstract::Several of the song control nuclei of songbirds, including HVc (higher vocal center) and Area X, contain melatonin receptor (MelR). In laboratory-housed male starlings, the densities of MelR in Area X change markedly according to reproductive state. MelR are down-regulated when starlings are photostimulated (in full b...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
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abstract::This review focuses on the endocrine regulation of reproductive behavior in moth species with particular emphasis on Helicoverpa spp. Reproductive behavior in most adult moths is dependent on the release of a unique blend of sex pheromones by the females to attract conspecific males. Mating, on the other hand, results...
journal_title:General and comparative endocrinology
pub_type: 杂志文章,评审
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abstract::The maternal control directing the very first hours of life is of pivotal importance for ensuring proper development to the growing embryo. Thanks to the finely regulated inheritance of maternal factors including mRNAs and proteins produced during oogenesis and stored into the mature oocyte, the embryo is sustained th...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2016.03.028
更新日期:2017-05-01 00:00:00
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journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2015.01.012
更新日期:2015-10-01 00:00:00
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journal_title:General and comparative endocrinology
pub_type: 杂志文章
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更新日期:1988-03-01 00:00:00
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journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(84)90212-0
更新日期:1984-04-01 00:00:00
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journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2009.08.008
更新日期:2010-03-01 00:00:00
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journal_title:General and comparative endocrinology
pub_type: 杂志文章
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更新日期:2019-11-01 00:00:00
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journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1006/gcen.1996.6871
更新日期:1997-05-01 00:00:00
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journal_title:General and comparative endocrinology
pub_type: 杂志文章
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更新日期:2014-01-01 00:00:00
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journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1006/gcen.1993.1179
更新日期:1993-12-01 00:00:00
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journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2018.09.014
更新日期:2018-12-01 00:00:00
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journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(84)90112-6
更新日期:1984-08-01 00:00:00
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journal_title:General and comparative endocrinology
pub_type: 杂志文章
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journal_title:General and comparative endocrinology
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journal_title:General and comparative endocrinology
pub_type: 杂志文章,评审
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journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2009.07.006
更新日期:2010-01-15 00:00:00
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journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1006/gcen.1994.1018
更新日期:1994-02-01 00:00:00
abstract::Testosterone- and progesterone-like substances were detected by radioimmunoassay (RIA) in chromatographed extracts of hemolymph from larvae of Sarcophaga bullata (S.B.). Gas chromatographic (GC) analysis after heptafluorobutyric acid (HFBA) derivation of hemolymph extracts, purified by paper and silica gel column chro...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(83)90175-2
更新日期:1983-12-01 00:00:00
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journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(88)90029-9
更新日期:1988-03-01 00:00:00
abstract::Prostaglandin F2 alpha (PGF2 alpha), progesterone, androgens (testosterone + dihydrotestosterone), and 17 beta-estradiol were measured in the plasma of male frogs, Rana esculenta, by radioimmunoassays. Plasma concentrations of PGF2 alpha were higher from October to December and peaked in March (prereproduction) and in...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(91)90307-r
更新日期:1991-06-01 00:00:00
abstract::Fish can respond to stimuli from the internal or external environment with activation of the hypothalamo-pituitary-interrenal (HPI) axis and the secretion of cortisol. Stimuli that activate the HPI axis of fish include short term air exposure and increases in water temperature. The present study was conducted to deter...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2018.09.017
更新日期:2019-01-01 00:00:00
abstract::Two peptides with sequence similarities to growth hormone releasing hormone (GHRH) have been identified by analysis of the chicken genome. One of these peptides, chicken (c) GHRH-LP (like peptide) was previously found to poorly bind to chicken pituitary membranes or to cloned and expressed chicken GHRH receptors and h...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2014.06.007
更新日期:2014-08-01 00:00:00
abstract::In fish, luteinizing hormone (LH) stimulates processes leading to final oocyte maturation and ovulation in females, and spermiation in males. The hormone is a heterodimeric glycoprotein composed of two non-covalently associated subunits. In this study, we describe the expression of tilapia LH (tLH) as a biologically a...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2004.10.016
更新日期:2005-02-01 00:00:00
abstract::To clarify the physiological roles of the renin-angiotensin-aldosterone system (RAAS) and arginine vasotocin (AVT) on body fluid regulation in amphibians, we measured plasma concentrations of aldosterone (ALDO), angiotensin II (ANG II), and AVT after various osmotic challenges in the marine toad, Bufo marinus (Bufonid...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2004.10.005
更新日期:2005-01-15 00:00:00