Abstract:
:TP53 accumulation in human primary breast carcinomas was studied by a quantitative luminometric immunoassay (LIA), and TP53 gene alterations, exons 5-8, were examined by single-strand conformation polymorphism (SSCP) analysis. In 48 of 142 breast tumor samples, a TP53 gene alteration was identified. In tumor samples without a TP53 gene alteration, the median cytosolic TP53 protein level, as determined by LIA, was 0.4 ng/mg protein (range 0-70.8 ng/mg protein), whereas the median TP53 protein level for tumor samples with a TP53 gene alteration was 10 times higher, i.e., 4.1 ng/mg protein (range 0.1-176.0 ng/mg protein). Despite a significant correlation between the outcome of LIA and SSCP, a disagreement was found in 22% of cases analyzed. Significant correlations were found between TP53 protein accumulation and low estrogen receptor content, and with a shorter relapse-free as well as overall survival, with a median duration of follow-up of 100 months. Due to its rapid and easy performance on routinely prepared cytosols, the LIA for TP53 protein may be useful in evaluating the prognostic impact of TP53 protein accumulation in human primary breast cancer.
journal_name
Int J Cancerjournal_title
International journal of cancerauthors
de Witte HH,Foekens JA,Lennerstrand J,Smid M,Look MP,Klijn JG,Benraad TJ,Berns EMdoi
10.1002/(SICI)1097-0215(19960422)69:2<125::AID-IJCsubject
Has Abstractpub_date
1996-04-22 00:00:00pages
125-30issue
2eissn
0020-7136issn
1097-0215pii
10.1002/(SICI)1097-0215(19960422)69:2<125::AID-IJCjournal_volume
69pub_type
杂志文章abstract::The prevalence of adult T-cell-leukemia virus (ATLV) infection was examined in Japanese monkeys living naturally in various parts of Japan and in other species of non-human primates imported into and kept in Japan. Sera of 2,650 Japanese monkeys from 41 troops throughout Japan were tested. High incidences of anti-ATLV...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910330205
更新日期:1984-02-15 00:00:00
abstract::The topic of this review is the role of stromal-epithelial interactions in normal and malignant prostatic growth. Because cell-cell interactions and androgens play such key roles in the prostate, the goal of this review will be to apply endocrinologic and developmental concepts to the understanding of normal and malig...
journal_title:International journal of cancer
pub_type: 杂志文章,评审
doi:10.1002/ijc.11335
更新日期:2003-10-20 00:00:00
abstract::Laser scanning confocal microscopy has been used to follow the uptake and efflux of the 2-fluoroglycoside of doxorubicin, ME2303, in live cultures of the human ovarian cancer cell line A2780 and its doxorubicin-resistant variant A2780AD. Our methods combine confocal laser scanning microscopy and image analysis to exam...
journal_title:International journal of cancer
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doi:10.1002/ijc.2910590118
更新日期:1994-10-01 00:00:00
abstract::Familial atypical multiple mole melanoma (FAMMM) is an autosomal dominant disease characterized by the familial occurrence of malignant melanoma of the skin and multiple atypical precursor lesions. Germline mutations in the p16 (CDKN2A) gene have been reported in at least a quarter of such families. An association has...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:
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abstract::Activation-induced cytidine deaminase (AID) plays a role as a genome mutator in activated B cells, and inappropriate expression of AID has been implicated in the immunopathological phenotype of human B-cell malignancies. Notably, we found that the transgenic mice overexpressing AID developed lung adenocarcinoma and he...
journal_title:International journal of cancer
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doi:10.1002/ijc.22292
更新日期:2007-02-01 00:00:00
abstract::The open reading frames of the phosphoprotein pp58 (BMRFI) and the deoxyribonuclease (BGLF5) of the Epstein-Barr-virus (EBV) strain M-ABA were cloned in the baculovirus expression vectors pAc373 and pAc360 and expressed in the Spodoptera frugiperda (SF158) insect cells. The recombinant phosphoprotein pp58 expressed in...
journal_title:International journal of cancer
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journal_title:International journal of cancer
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更新日期:1999-08-12 00:00:00
abstract::Previous studies have revealed a robust association between exposure to asbestos and human lung cancer. Accumulating evidence has highlighted the role of epigenome deregulation in the mechanism of carcinogen-induced malignancies. We examined the impact of asbestos on DNA methylation. Our genome-wide studies (using Ill...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.30897
更新日期:2017-11-15 00:00:00
abstract::The UBE1L gene isolated from the chromosome 3p21 region has an extremely reduced level of mRNA in lung cancer. Sequence analysis showed a 45% homology to the human ubiquitin-activating enzyme E1 at the amino acid level. To further characterize the protein product, we generated UBE1L protein-specific antibodies. Immuno...
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doi:10.1002/(sici)1097-0215(20000315)85:6<871::aid-ijc
更新日期:2000-03-15 00:00:00
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journal_title:International journal of cancer
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doi:10.1002/(sici)1097-0215(19990924)83:1<10::aid-ijc3
更新日期:1999-09-24 00:00:00
abstract::Increased exposure to estrogen is a risk factor for postmenopausal breast cancer, and dietary factors can influence estrogen metabolism. However, studies of diet and breast cancer have been inconclusive. We developed a dietary pattern associated with levels of unconjugated estradiol and the ratio of 2- and 16-hydroxyl...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.31387
更新日期:2018-08-01 00:00:00
abstract::To estimate the annual incidence of adult T-cell leukemia/lymphoma (ATL) by district in Japan, a large-scale nationwide survey of ATL and of non-Hodgkin's lymphoma was performed in 1988. Questionnaires for the registration of ATL and of T-cell and non-T-cell lymphoma were distributed to the physicians in charge of thi...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910450206
更新日期:1990-02-15 00:00:00
abstract::We have employed the DNA-mediated gene transfer method to introduce the allogenic major-histocompatibility-complex(MHC)-class-I gene H-2Kb into the K36.16 tumor cells, H-2k, in order to generate tumor-specific immunity. The acquisition of immunogenicity by the H-2Kb-transformed clones following gene transfer is associ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910570215
更新日期:1994-04-15 00:00:00
abstract::Human papilloma virus is a causative factor in the etiology of cervical cancer with HPV16 being the most prevalent genotype associated with it. Intratype variations in oncogenic E6/E7 and capsid L1 proteins of HPV 16 besides being of phylogenetic importance, are associated with risk of viral persistence and progressio...
journal_title:International journal of cancer
pub_type: 杂志文章,多中心研究
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更新日期:2009-07-01 00:00:00
abstract::DNA copy number alterations (CNAs) are frequent in cancer, and recently developed CNA signatures revealed their value in molecular tumor stratification for patient prognosis and platinum resistance prediction in ovarian cancer. Head and neck squamous cell carcinoma (HNSCC) is also characterized by high CNAs. In this s...
journal_title:International journal of cancer
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更新日期:2020-09-15 00:00:00
abstract::The cellular tumor suppressor p16 is strongly overexpressed in cervical cancers and precancers. We have previously demonstrated that infiltrating T lymphocytes reactive against p16 can be found in cervical cancer patients. Here, we analyzed whether p16 induces humoral immune responses. Sera of patients with cervical c...
journal_title:International journal of cancer
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doi:10.1002/ijc.23837
更新日期:2008-12-01 00:00:00
abstract::Two hundred and twelve archival bladder-cancer biopsy specimens were analyzed immunohistochemically analysis were correlated to established histological and quantitative prognostic factors and survival of patients during a mean follow-up period of more than 10 years. Twenty-nine percent of tumours were positive for p5...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910530304
更新日期:1993-02-01 00:00:00
abstract::The purpose of this analysis was to correlate isotope selection with the urinary symptoms of patients who received a combination of external beam radiotherapy (EBRT) and a transperineal interstitial permanent prostate brachytherapy (TIPPB) boost with either a (103)palladium ((103)Pd) or a (125)iodine ((125)I) radioiso...
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abstract::A population-based case-control study was performed to evaluate the risk of endometrial cancer related to tamoxifen or toremifene treatment. All patients with breast cancer diagnosis since 1980 in Finland who subsequently developed an endometrial cancer by the end of 1995 and 3 matched controls were identified among t...
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journal_title:International journal of cancer
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更新日期:1994-11-01 00:00:00
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journal_title:International journal of cancer
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journal_title:International journal of cancer
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更新日期:2005-12-10 00:00:00
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journal_title:International journal of cancer
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更新日期:1989-11-15 00:00:00
abstract::In a hospital-based case-control study of esophageal cancer undertaken in Athens (1989-1991), 43 patients with incident esophageal squamous-cell carcinoma and 56 patients with incident esophageal adenocarcinoma were compared to 200 injury patients. Personal interviews were conducted in the hospital setting, and dietar...
journal_title:International journal of cancer
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更新日期:1996-11-04 00:00:00
abstract::Differentiated thyroid carcinoma (TC) is threefold more common in women than in men and, therefore, a role of female hormones in the etiology of differentiated TC has been suggested. We assessed these hypotheses in the European Prospective Investigation into Cancer and Nutrition (EPIC) cohort. Among 345,157 women (mea...
journal_title:International journal of cancer
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abstract::Vitamin D has anticarcinogenic properties and might influence colorectal cancer (CRC) risk, but the epidemiological evidence is inconsistent. Many mechanisms of action for vitamin D have been proposed, with some of them initiating via its binding to the vitamin D receptor (VDR). Using a large Scottish case-control stu...
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abstract::BRCA1 and BRCA2 play essential roles in maintaining the genome stability. Pathogenic germline mutations in these two genes disrupt their function, lead to genome instability and increase the risk of developing breast and ovarian cancers. BRCA mutations have been extensively screened in Caucasian populations, and the r...
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abstract::An important feature of cytochrome P450 (CYP) 2B1 is its high ability to convert the prodrug cyclophosphamide (CPA) to therapeutically cytotoxic metabolites, resulting in interstrand DNA-cross-linking and cell death. We have examined whether and how the phosphorylation of CYP2B1 influences CPA metabolic activation in ...
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