Prognostic significance of TP53 accumulation in human primary breast cancer: comparison between a rapid quantitative immunoassay and SSCP analysis.


:TP53 accumulation in human primary breast carcinomas was studied by a quantitative luminometric immunoassay (LIA), and TP53 gene alterations, exons 5-8, were examined by single-strand conformation polymorphism (SSCP) analysis. In 48 of 142 breast tumor samples, a TP53 gene alteration was identified. In tumor samples without a TP53 gene alteration, the median cytosolic TP53 protein level, as determined by LIA, was 0.4 ng/mg protein (range 0-70.8 ng/mg protein), whereas the median TP53 protein level for tumor samples with a TP53 gene alteration was 10 times higher, i.e., 4.1 ng/mg protein (range 0.1-176.0 ng/mg protein). Despite a significant correlation between the outcome of LIA and SSCP, a disagreement was found in 22% of cases analyzed. Significant correlations were found between TP53 protein accumulation and low estrogen receptor content, and with a shorter relapse-free as well as overall survival, with a median duration of follow-up of 100 months. Due to its rapid and easy performance on routinely prepared cytosols, the LIA for TP53 protein may be useful in evaluating the prognostic impact of TP53 protein accumulation in human primary breast cancer.


Int J Cancer


de Witte HH,Foekens JA,Lennerstrand J,Smid M,Look MP,Klijn JG,Benraad TJ,Berns EM




Has Abstract


1996-04-22 00:00:00














  • Hot maté drinking and precancerous lesions of the oesophagus: an endoscopic survey in southern Brazil.

    abstract::Thermal injury resulting from drinking very hot beverages has been incriminated as a risk factor for oesophageal cancer, although no information is available on the lesions caused by this injury in human or experimental animals. The drinking of hot maté tea is very common in areas of moderately high incidence of oesop...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Muñoz N,Victora CG,Crespi M,Saul C,Braga NM,Correa P

    更新日期:1987-06-15 00:00:00

  • Smoking, snus use and risk of right- and left-sided colon, rectal and anal cancer: a 37-year follow-up study.

    abstract::Although some authorities consider smoking to be an established risk factor for colorectal cancer, the international literature is not entirely consistent. Further, only 1 study has addressed the association with smokeless tobacco and none with Scandinavian moist snuff (snus). This retrospective cohort study included ...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Nordenvall C,Nilsson PJ,Ye W,Nyrén O

    更新日期:2011-01-01 00:00:00

  • Cervical cancer cell-derived interleukin-6 impairs CCR7-dependent migration of MMP-9-expressing dendritic cells.

    abstract::Cervical carcinogenesis is a consequence of persistent infection with high-risk human papillomaviruses (HPVs). Recent studies indicate that HPV-transformed cells actively instruct their microenvironment to promote carcinogenesis. Here, we demonstrate that cervical cancer cells activate monocytes to produce their own C...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Pahne-Zeppenfeld J,Schröer N,Walch-Rückheim B,Oldak M,Gorter A,Hegde S,Smola S

    更新日期:2014-05-01 00:00:00

  • Impact of p53-based immunization on primary chemically-induced tumors.

    abstract::In mice as well as humans, cytotoxic T lymphocytes (CTL) specific for wild-type-sequence (wt) p53 peptides have been shown to react against a wide range of tumors, but not normal cells. As such, they are attractive candidates for developing broadly applicable cancer vaccines. Of particular interest is the potential of...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Cicinnati VR,Dworacki G,Albers A,Beckebaum S,Tüting T,Kaczmarek E,DeLeo AB

    更新日期:2005-03-01 00:00:00

  • Fetal myofibroblast-like cells isolated from post-radiation fibrosis in human breast cancer.

    abstract::Cells were isolated from post-radiation fibrosis biopsies of patients with recurrent breast carcinoma. These cells were identified as fibroblasts and compared with fibroblasts from normal breast tissues for their proliferative activities, chromosome number and for the presence of various components of the extracellula...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Brouty-Boyé D,Raux H,Azzarone B,Tamboise A,Tamboise E,Béranger S,Magnien V,Pihan I,Zardi L,Israël L

    更新日期:1991-03-12 00:00:00

  • Trends in seroprevalence of human papillomavirus type 16 among pregnant women in Stockholm, Sweden, during 1969-1989.

    abstract::To assess long-term trends in the prevalence of oncogenic human papillomavirus (HPV) infection, we performed a cross-sectional serosurvey of the seroprevalence of the major oncogenic HPV type, HPV16, among 3,512 pregnant women undergoing population-based serological screening at the first trimester of pregnancy in the...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: af Geijersstam V,Wang Z,Lewensohn-Fuchs I,Eklund C,Schiller JT,Forsgren M,Dillner J

    更新日期:1998-05-04 00:00:00

  • Suppression of pancreatic cancer growth and metastasis by HMP19 identified through genome-wide shRNA screen.

    abstract::Therapeutic effectiveness against metastatic or even locally advanced pancreatic ductal adenocarcinoma (PDAC) is dismal, with 5-year survival less than 5%. Even in patients who undergo potentially curative resection, most patients' tumors recur in the liver. Improving therapies targeting or preventing liver metastases...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Kurahara H,Bohl C,Natsugoe S,Nishizono Y,Harihar S,Sharma R,Iwakuma T,Welch DR

    更新日期:2016-08-01 00:00:00

  • Endogenous sex hormones and the risk of prostate cancer: a prospective study.

    abstract::Sex steroid hormones influence prostate development and maintenance through their roles in prostate cellular proliferation, differentiation and apoptosis. Although suspected to be involved in prostate carcinogenesis, an association between circulating androgens and prostate cancer has not been clearly established in e...

    journal_title:International journal of cancer

    pub_type: 杂志文章,随机对照试验


    authors: Weiss JM,Huang WY,Rinaldi S,Fears TR,Chatterjee N,Hsing AW,Crawford ED,Andriole GL,Kaaks R,Hayes RB

    更新日期:2008-05-15 00:00:00

  • CD66b, CD66c and carcinoembryonic antigen (CEA) are independently regulated markers in sera of tumor patients.

    abstract::Non-specific cross-reacting antigens (NCA-95 = CD66b and NCA-50/90 = CD66c) are members of the CEA (carcinoembryonic antigen = CD66e) family. Analysis of mRNA levels of CD66c in colon tumors suggests that this antigen is strongly up-regulated compared to its normal counterpart and could, therefore, be of clinical inte...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Grunert F,Daniel S,Nagel G,von Kleist S,Jantscheff P

    更新日期:1995-11-03 00:00:00

  • [fam-] trastuzumab deruxtecan, antitumor activity is dependent on HER2 expression level rather than on HER2 amplification.

    abstract::Therapies targeted to human epidermal growth factor receptor 2 (HER2) have proven effective against tumors positive for HER2 amplification, but there is an unmet clinical need for the treatment of tumors that express HER2 protein in the absence of HER2 amplification. [fam-] trastuzumab deruxtecan (DS-8201a) is a novel...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Takegawa N,Tsurutani J,Kawakami H,Yonesaka K,Kato R,Haratani K,Hayashi H,Takeda M,Nonagase Y,Maenishi O,Nakagawa K

    更新日期:2019-12-15 00:00:00

  • Diet and risk of esophageal cancer by histologic type in a low-risk population.

    abstract::In a hospital-based case-control study of esophageal cancer undertaken in Athens (1989-1991), 43 patients with incident esophageal squamous-cell carcinoma and 56 patients with incident esophageal adenocarcinoma were compared to 200 injury patients. Personal interviews were conducted in the hospital setting, and dietar...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Tzonou A,Lipworth L,Garidou A,Signorello LB,Lagiou P,Hsieh C,Trichopoulos D

    更新日期:1996-11-04 00:00:00

  • Risk factors for esophageal cancer in Shanghai, China. I. Role of cigarette smoking and alcohol drinking.

    abstract::A population-based case-control study of esophageal cancer was conducted in urban Shanghai involving interviews with 902 cases and 1,552 controls. Risk of esophageal cancer was increased among tobacco smokers and alcohol drinkers. Odds ratios (OR) for smoking were 2.1 and 1.6 for men and women, respectively, and incre...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Gao YT,McLaughlin JK,Blot WJ,Ji BT,Benichou J,Dai Q,Fraumeni JF Jr

    更新日期:1994-07-15 00:00:00

  • Cholera-toxin-enhanced growth of human breast cancer cell lines in vitro and in vivo: interaction with estrogen.

    abstract::Cholera toxin (which increases intracellular cAMP levels) significantly (p less than 0.05) increased the growth of MCF-7, T47-D and Hs578T human breast carcinoma cells in vitro. The effect of cholera toxin on growth of MCF-7 and T47-D cells was more pronounced in the presence of 17 beta-estradiol (p less than 0.05), i...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Sheffield LG,Welsch CW

    更新日期:1985-10-15 00:00:00

  • The C-terminus of apoptin represents a unique tumor cell-enhanced nuclear targeting module.

    abstract::Chicken anemia virus viral protein 3 (VP3 or apoptin) localizes more efficiently in the nucleus of transformed than nontransformed cells. Although previous studies implicate the C-terminus of apoptin as being responsible, the molecular basis is controversial, and the extent to which altered nuclear transport efficienc...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Kuusisto HV,Wagstaff KM,Alvisi G,Jans DA

    更新日期:2008-12-15 00:00:00

  • Novel melanoma antigen, FCRL/FREB, identified by cDNA profile comparison using DNA chip are immunogenic in multiple melanoma patients.

    abstract::We applied a strategy that utilized a combination of systematic gene expression analysis with various tissues and immunological detection with sera from melanoma patients to identify melanoma antigens expressed preferentially in melanoma and melanocytes. We selected 101 genes by comparing cDNA profiles obtained by Gen...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Inozume T,Matsuzaki Y,Kurihara S,Fujita T,Yamamoto A,Aburatani H,Shimada S,Kawakami Y

    更新日期:2005-03-20 00:00:00

  • Comparison of DNA hypermethylation patterns in different types of uterine cancer: cervical squamous cell carcinoma, cervical adenocarcinoma and endometrial adenocarcinoma.

    abstract::The incidence of cervical adenocarcinoma (CA) is rising, whereas the incidence of cervical squamous cell carcinoma (CSCC) continues to decrease. However, it is still unclear whether different molecular characteristics underlie these 2 types of cervical carcinoma. To better understand the epigenetic characteristics of ...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Kang S,Kim JW,Kang GH,Lee S,Park NH,Song YS,Park SY,Kang SB,Lee HP

    更新日期:2006-05-01 00:00:00

  • Acquired resistance towards immune defense during metastatic progression represents a secondary phenomenon.

    abstract::The role of natural immune defense in the control of metastatic spread of tumor cells was evaluated by adapting an s.c.-grown, antigenic, but non-immunogenic rat fibrosarcoma (Bsp6S) to ascitic growth (BSp6A) in the strain of origin (BDX), where peritoneal cells display a high level of NK and macrophage activity. Para...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Zöller M

    更新日期:1986-01-15 00:00:00

  • Microsatellite instability and pathological aspects of breast cancer.

    abstract::Genomic instability plays a key role in hereditary nonpolyposis colorectal cancer and in a significant sub-set of non-hereditary colorectal tumors. Recent evidence suggests that microsatellite instability also occurs in various sub-sets of common, non-hereditary forms of extra-colorectal carcinoma. To investigate the ...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Contegiacomo A,Palmirotta R,De Marchis L,Pizzi C,Mastranzo P,Delrio P,Petrella G,Figliolini M,Bianco AR,Frati L

    更新日期:1995-08-22 00:00:00

  • Parity, age at first birth and the risk of carcinoma in situ of the breast.

    abstract::Epidemiological studies of in situ breast cancer are sparse, and the role of reproductive history, an established risk modifier for invasive breast cancer, remains incompletely investigated. To examine possible associations with parity and age at first birth, we undertook a case-control study nested in a nationwide co...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Lambe M,Hsieh CC,Tsaih SW,Ekbom A,Trichopoulos D,Adami HO

    更新日期:1998-07-29 00:00:00

  • Radiation-related mortality among offspring of atomic bomb survivors: a half-century of follow-up.

    abstract::Our objective was to examine whether parental exposure to atomic bomb radiation has led to increased cancer and/or noncancer mortality rates among the offspring. We studied 41,010 subjects born from May 1946 through December 1984 (i.e., conceived between 1 month and 38 years after the bombings) and surviving for at le...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Izumi S,Suyama A,Koyama K

    更新日期:2003-11-01 00:00:00

  • Mouse mammary tumor virus production stimulated by hormones and polyamines in cells grown in semi-synthetic in vitro conditions.

    abstract::Hormonal regulation of mouse mammary tumor virus (MMTV) synthesis was studied in the CCL-51-SF cell subline derived from the Sykes' mammary tumor cell line CCL-51 and adapted to grow in semi-synthetic in vitro conditions. The virus was quantitated by measuring the supernatant reverse transcriptase activity in exogenou...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Svec J,Links J

    更新日期:1977-02-15 00:00:00

  • Impact of BCRP/MXR, MRP1 and MDR1/P-Glycoprotein on thermoresistant variants of atypical and classical multidrug resistant cancer cells.

    abstract::The impact of the ABC transporters breast cancer resistance protein/mitoxantrone resistance associated transporter (BCRP/MXR), multidrug resistance-associated protein 1 (MRP1) and multidrug resistance gene-1/P-glycoprotein (MDR1/PGP) on the multidrug resistance (MDR) phenotype in chemoresistance and thermoresistance w...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Stein U,Lage H,Jordan A,Walther W,Bates SE,Litman T,Hohenberger P,Dietel M

    更新日期:2002-02-20 00:00:00

  • Relationship between ornithine decarboxylase levels in anaplastic gliomas and progression-free survival in patients treated with DFMO-PCV chemotherapy.

    abstract::The purpose of this study was to assess the relationship between progression-free survival (PFS) in patients treated with DFMO + PCV (procarbazine, CCNU, vincristine) chemotherapy for malignant gliomas with tumor cell ornithine decarboxylase (ODC) activity. Formalin-fixed slides were obtained for study patients with a...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Levin VA,Jochec JL,Shantz LM,Aldape KD

    更新日期:2007-11-15 00:00:00

  • Hypermethylation of RASSF1A and BLU tumor suppressor genes in non-small cell lung cancer: implications for tobacco smoking during adolescence.

    abstract::The putative tumor suppressors RASSF1A and BLU are mapped adjacent to one another on chromosome 3p21.3, a region frequently deleted in lung cancer. These genes are often inactivated by promoter hypermethylation, but the association of this inactivation with clinical features of the disease or with carcinogen exposure ...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Marsit CJ,Kim DH,Liu M,Hinds PW,Wiencke JK,Nelson HH,Kelsey KT

    更新日期:2005-03-20 00:00:00

  • Association of a functional polymorphism in the promoter of the MDM2 gene with risk of nonsmall cell lung cancer.

    abstract::Lung cancer is the leading cause of cancer mortality in the world. Although exposure to carcinogens is considered to be the main cause, genetic variation may contribute to lung cancer risk. Murine double minute 2, MDM2, is a key regulator of p53 activity and recently a polymorphism in the promoter region of the MDM2 g...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Lind H,Zienolddiny S,Ekstrøm PO,Skaug V,Haugen A

    更新日期:2006-08-01 00:00:00

  • Transcription factor co-repressors in cancer biology: roles and targeting.

    abstract::Normal transcription displays a high degree of flexibility over the choice, timing and magnitude of mRNA expression levels that tend to oscillate and cycle. These processes allow for combinatorial actions, feedback control and fine-tuning. A central role has emerged for the transcriptional co-repressor proteins such a...

    journal_title:International journal of cancer

    pub_type: 杂志文章,评审


    authors: Battaglia S,Maguire O,Campbell MJ

    更新日期:2010-06-01 00:00:00

  • HOXA11 DNA methylation--a novel prognostic biomarker in ovarian cancer.

    abstract::Epigenetic alterations play a major role in cancer. Recently we reported that stem cell Polycomb group targets (PcGTs) are up to 12-fold more likely to have cancer-specific promoter DNA hypermethylation than nontargets. To identify potential, prognostic DNA methylation markers in ovarian cancer we analyzed the DNA met...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Fiegl H,Windbichler G,Mueller-Holzner E,Goebel G,Lechner M,Jacobs IJ,Widschwendter M

    更新日期:2008-08-01 00:00:00

  • Rapid quantification of HTLV-I provirus load: detection of monoclonal proliferation of HTLV-I-infected cells among blood donors.

    abstract::In this report, we quantified HTLV-I provirus load using the AmpliSensor system, which utilizes fluorescence to measure PCR products. With this method, provirus loads could be measured within 6 hr, and the results obtained correlated well with those obtained by other methods. Samples from 256 blood donors, who were po...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Etoh K,Yamaguchi K,Tokudome S,Watanabe T,Okayama A,Stuver S,Mueller N,Takatsuki K,Matsuoka M

    更新日期:1999-06-11 00:00:00

  • Activation of protein kinase C modulates cell-cell and cell-substratum adhesion of a human colorectal carcinoma cell line and restores 'normal' epithelial morphology.

    abstract::Abnormal cell adhesion is an important contributing factor in invasion and metastasis. Here, we show that morphologically 'normal' cell-cell and cell-substratum adhesion can be restored to a poorly differentiated carcinoma cell line by activation of protein kinase C (PKC). This cell line, VACO 10MS, grows as multicell...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Cowell HE,Garrod DR

    更新日期:1999-01-29 00:00:00

  • Fried potatoes and human cancer.

    abstract::A considerable public concern about cancer risk from acrylamide-rich foods followed the announcement that high concentrations of acrylamide are found in fried potatoes and potato chips and, more generally, in starch-containing foods cooked at high temperatures. From a series of hospital-based case-control studies cond...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Pelucchi C,Franceschi S,Levi F,Trichopoulos D,Bosetti C,Negri E,La Vecchia C

    更新日期:2003-07-01 00:00:00