Molecular cloning, membrane topology, and localization of bovine rom-1 in rod and cone photoreceptor cells.

abstract：PURPOSE:To examine the behavior of fibroblasts and retinal pigment epithelial cells after attachment to the retinal surface in vitro to elucidate the pathobiology of the early stages of epiretinal membrane formation. METHODS:Human retinal pigment epithelial (HRPE) cells and bovine Tenon's capsule fibroblasts (BTFs) we...

journal_title：Investigative ophthalmology & visual science

authors： Allamby D,Foreman D,Carrington L,McLeod D,Boulton M

更新日期：1997-09-01 00:00:00

abstract：PURPOSE:To determine whether transcorneal electrical stimulation (TES) has neuroprotective effects on the photoreceptors, and whether it slows the rate of decrease of the electroretinogram (ERG) in rhodopsin P347L transgenic (Tg) rabbits. METHODS:Six-week-old Tg rabbits received TES through a contact lens electrode on...

journal_title：Investigative ophthalmology & visual science

authors： Morimoto T,Kanda H,Kondo M,Terasaki H,Nishida K,Fujikado T

更新日期：2012-06-28 00:00:00

abstract：PURPOSE:Diabetic retinopathy (DR) has features of chronic low-grade inflammation. The purpose of our study was to investigate whether the presence of inflammatory cells in fibrovascular membranes (FVMs) from patients with proliferative diabetic retinopathy (PDR) is associated with the activity of PDR and visual acuity ...

journal_title：Investigative ophthalmology & visual science

authors： Urbančič M,Štunf Š,Milutinović Živin A,Petrovič D,GlobočnikPetrovič M

更新日期：2014-11-25 00:00:00

abstract：PURPOSE:To improve the 30-year-old "trypsin digestion" procedure for isolation of the complete retinal vasculature, which, in its time, was a revolutionary advance that allowed important discoveries about diabetic retinopathy; to provide a method that will yield more consistent results when applied to retinas represent...

journal_title：Investigative ophthalmology & visual science

authors： Laver NM,Robison WG Jr,Pfeffer BA

更新日期：1993-05-01 00:00:00

abstract：Purpose:Synucleinopathies such as multiple system atrophy (MSA) and Parkinson's disease are associated with a variety of visual symptoms. Functional and morphological retinal aberrations are therefore supposed to be valuable biomarkers for these neurodegenerative diseases. This study examined the retinal morphology and...

journal_title：Investigative ophthalmology & visual science

authors： Kaehler K,Seitter H,Sandbichler AM,Tschugg B,Obermair GJ,Stefanova N,Koschak A

更新日期：2020-06-03 00:00:00

abstract：PURPOSE:Retinal dopamine deficiency is a potential cause of myopia and visual deficits in retinopathy of prematurity (ROP). We investigated the cellular mechanisms responsible for lowered levels of retinal dopamine in an oxygen-induced retinopathy (OIR) mouse model of ROP. METHODS:Retinopathy was induced by exposing m...

journal_title：Investigative ophthalmology & visual science

authors： Spix NJ,Liu LL,Zhang Z,Hohlbein JP,Prigge CL,Chintala S,Ribelayga CP,Zhang DQ

更新日期：2016-06-01 00:00:00

abstract：PURPOSE:The purpose of the present study was to investigate metabolic changes in different compartments of the rat lens (anterior, nuclear, posterior, and equatorial) after exposure to an acute double threshold dose of ultraviolet-B radiation (UVR-B) by using high-resolution magic angle spinning (HR-MAS) (1)H nuclear m...

journal_title：Investigative ophthalmology & visual science

authors： Tessem MB,Bathen TF,Löfgren S,Saether O,Mody V,Meyer L,Dong X,Söderberg PG,Midelfart A

更新日期：2006-12-01 00:00:00

abstract：PURPOSE:To determine the molecular mechanisms underlying the protective effects of zinc against oxidative stress in cultured retinal pigment epithelial (RPE) cells. METHODS:Cultured ARPE-19 cells were treated with different concentrations of zinc for various times. Cellular glutathione (GSH) and glutathione disulfide ...

journal_title：Investigative ophthalmology & visual science

authors： Ha KN,Chen Y,Cai J,Sternberg P Jr

更新日期：2006-06-01 00:00:00

abstract：PURPOSE:The goal of this study was to extend the results of previous immunoassay, immunocytochemistry, and in situ hybridization studies showing the presence of insulin-related peptide in the rat retina by confirming the expression of insulin genes in the rat eye. METHODS:Total and poly(A)+ RNA were isolated from whol...

journal_title：Investigative ophthalmology & visual science

authors： Budd GC,Pansky B,Glatzer L

更新日期：1993-02-01 00:00:00

abstract：PURPOSE:A human biovar of Chlamydia trachomatis was used to develop a murine model of ocular chlamydial infection. The inbred mouse model will allow detailed immunologic studies during ocular infection, and use of a human biovar for infection may aid in identification of appropriate vaccine strategies against chlamydia...

journal_title：Investigative ophthalmology & visual science

authors： Whittum-Hudson JA,O'Brien TP,Prendergast RA

更新日期：1995-09-01 00:00:00

abstract：PURPOSE:To investigate the effect of etomidate (ET) on axonal regeneration of retinal ganglion cells (RGCs) in adult rats. METHODS:The optic nerve was transected intraorbitally at 1 mm from the optic disc, and an autologous peripheral nerve was transplanted onto the ocular ON stump in adult rats. Then the animals were...

journal_title：Investigative ophthalmology & visual science

authors： Xu ZX,Qin SZ,Xu GZ,Hu JM,Ma LT

更新日期：2011-10-11 00:00:00

abstract：PURPOSE:To investigate macular changes in acute retinopathy of prematurity (ROP). METHODS:Fifty-four premature infants with ROP and 20 controls underwent routine ROP screening with indirect ophthalmoscopy and imaging. A tabletop spectral domain optical coherence tomography (SD-OCT) scanner (Spectralis; Heidelberg Engi...

journal_title：Investigative ophthalmology & visual science

authors： Vinekar A,Avadhani K,Sivakumar M,Mahendradas P,Kurian M,Braganza S,Shetty R,Shetty BK

更新日期：2011-07-15 00:00:00

abstract：PURPOSE:To assess presbyopic spectacle coverage, willingness to pay, and the impact of correcting uncorrected presbyopia in individuals 40 years of age and older in Zanzibar and whether assessment of presbyopia can be incorporated into Rapid Assessment of Blindness (RAAB) surveys. METHODS:A cross-sectional, population...

journal_title：Investigative ophthalmology & visual science

authors： Laviers HR,Omar F,Jecha H,Kassim G,Gilbert C

更新日期：2010-02-01 00:00:00

abstract：PURPOSE:The purpose of this study was to examine ocular surface and tear film, contact lens, care solution, medical, and patient-related factors that are associated with corneal staining in contact lens wearers. METHODS:In this cross-sectional/nested case-control study, in addition to the assessment of corneal stainin...

journal_title：Investigative ophthalmology & visual science

authors： Nichols JJ,Sinnott LT

更新日期：2011-02-28 00:00:00

abstract：PURPOSE:To evaluate the importance of lens epithelial cell death in age-related cataract. To determine whether the large percentage of terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick-end labeling (TUNEL)-positive lens epithelial cells previously reported in human capsulotomy specimens results from apopto...

journal_title：Investigative ophthalmology & visual science

authors： Harocopos GJ,Alvares KM,Kolker AE,Beebe DC

更新日期：1998-12-01 00:00:00

abstract：Purpose:We investigate the efficacy of a novel dichoptic mapping paradigm in evaluating visual function of anisometropic amblyopes. Methods:Using standard clinical measures of visual function (visual acuity, stereo acuity, Bagolini lenses, and neutral density filters) and a novel quantitative mapping technique, 26 pat...

journal_title：Investigative ophthalmology & visual science

authors： Li J,Li J,Chen Z,Liu J,Yuan J,Cai X,Deng D,Yu M

更新日期：2017-01-01 00:00:00

abstract：PURPOSE:In glaucoma, the optic nerve head (ONH) is the principal site of initial axonal injury, and elevated intraocular pressure (IOP) is the predominant risk factor. However, the initial responses of the ONH to elevated IOP are unknown. Here the authors use a rat glaucoma model to characterize ONH gene expression cha...

journal_title：Investigative ophthalmology & visual science

authors： Johnson EC,Doser TA,Cepurna WO,Dyck JA,Jia L,Guo Y,Lambert WS,Morrison JC

更新日期：2011-01-25 00:00:00

abstract：PURPOSE:To isolate and characterize ciliary body epithelial antigens reactive with a monoclonal antibody, 2B4.14.1. METHODS:A mouse monoclonal antibody generated against human corneal endothelium, 2B4.14.1 reacts with nonpigmented epithelium of human and guinea pig ciliary bodies. The ciliary body proteins reactive wi...

journal_title：Investigative ophthalmology & visual science

authors： Howell DN,Ross JR,Miller SE,Sanfilippo FP

更新日期：1994-07-01 00:00:00

abstract：PURPOSE:To determine whether vascular tone of isolated porcine retinal veins can be modulated by tissue-generated vasoactive factors such as endothelin-1 and adenosine. Such information may be useful in understanding the role of the retinal veins in regulating blood flow, and also provide a model for investigating new ...

journal_title：Investigative ophthalmology & visual science

authors： Yu DY,Su EN,Cringle SJ,Morgan WH,McAllister IL,Yu PK

更新日期：2016-02-01 00:00:00

abstract：PURPOSE:To determine whether adenovirus vectors are capable of transferring a foreign active protein to the perfused anterior segment of the human eye. METHODS:Primary cultures from the human trabecular meshwork tissue were exposed to replication-deficient adenovirus Av1LacZ4 carrying the reporter beta-galactosidase g...

journal_title：Investigative ophthalmology & visual science

authors： Borrás T,Matsumoto Y,Epstein DL,Johnson DH

更新日期：1998-07-01 00:00:00

abstract：PURPOSE:To investigate the effects of reducing connexin (Cx) diversity in the lens when the amount of connexin protein is nearly constant. METHODS:Lenses in which the Cx50 coding region was replaced by that of Cx46 (knockin [KI]), were compared with wild type (WT) and Cx50-knockout (KO) lenses. Gap junctional conducta...

journal_title：Investigative ophthalmology & visual science

authors： Martinez-Wittinghan FJ,Sellitto C,White TW,Mathias RT,Paul D,Goodenough DA

更新日期：2004-10-01 00:00:00

abstract：PURPOSE:This study examined the association between peripapillary choroidal thickness (PCT) with age and race in a group of African descent (AD) and European descent (ED) subjects with normal eyes. METHODS:Optic nerve head images from enhanced depth imaging spectral-domain optical coherence tomography of 166 normal ey...

journal_title：Investigative ophthalmology & visual science

authors： Rhodes LA,Huisingh C,Johnstone J,Fazio MA,Smith B,Wang L,Clark M,Downs JC,Owsley C,Girard MJ,Mari JM,Girkin CA

更新日期：2015-02-24 00:00:00

abstract：Purpose:Single-cell RNA-sequencing (scRNA-seq) was used to interrogate the relatively rare stem (SC) and early transit amplifying (TA) cell populations in limbal/corneal epithelia from wild-type and autophagy-compromised mice. Methods:We conducted scRNA-seq on ocular anterior segmental tissue from wild-type and beclin...

journal_title：Investigative ophthalmology & visual science

authors： Kaplan N,Wang J,Wray B,Patel P,Yang W,Peng H,Lavker RM

更新日期：2019-08-01 00:00:00

abstract：Purpose:The three-dimensional configurations of rod and cone bipolar cell (BC) dendrites and horizontal cell (HC) processes outside rod and cone synaptic terminals have not been fully elucidated. We reveal how these neurites are mutually arranged to coordinate formation and maintenance of the postsynaptic complex of ri...

journal_title：Investigative ophthalmology & visual science

authors： Tsukamoto Y,Iseki K,Omi N

更新日期：2021-01-04 00:00:00

abstract：Purpose:To assess the feasibility of macular integrity assessment (MAIA) microperimetry (MP) in children. Also to establish representative outcome measures (differential light sensitivity, fixation stability, test-retest reliability) for children without visual impairment. Methods:Thirty-three adults and 33 children (...

journal_title：Investigative ophthalmology & visual science

authors： Jones PR,Yasoubi N,Nardini M,Rubin GS

更新日期：2016-11-01 00:00:00

abstract：PURPOSE:Invasion of tumor cells into blood vessels is essential for metastasis of uveal melanoma. The occurrence of ingrowth of tumor cells in blood vessels in uveal melanoma was analyzed, and this parameter was compared with the survival of the patients. METHODS:Between 1972 and 2007, 643 eyes primarily enucleated fo...

journal_title：Investigative ophthalmology & visual science

authors： Ly LV,Odish OF,Wolff-Rouendaal Dd,Missotten GS,Luyten GP,Jager MJ

更新日期：2010-02-01 00:00:00

abstract：PURPOSE:To determine the influence of specific regions within Lp82 calpain on protein stability, enzymatic activity, and localization within lens and to test the influence of an Lp82 knockout mouse on normal maturational proteolysis in lens. METHODS:DNA constructs for Lp82 and Lp82-related proteins were subcloned into...

journal_title：Investigative ophthalmology & visual science

authors： Ma H,Shih M,Fukiage C,Azuma M,Duncan MK,Reed NA,Richard I,Beckmann JS,Shearer TR

更新日期：2000-12-01 00:00:00

abstract：:Topical application of Prostaglandin F2 alpha (PGF2 alpha) to the eye reduces intraocular pressure (IOP) in all mammalian species studied thus far, including humans. The L-isopropylester derivative is currently the one most commonly used in experimental and clinical studies. Dose-response relationships were determined...

journal_title：Investigative ophthalmology & visual science

authors： Crawford KS,Kaufman PL

更新日期：1991-03-01 00:00:00

abstract：PURPOSE:Docosahexaenoic acid (DHA) was supplemented in a single-site, placebo-controlled, randomized clinical trial designed to slow vision loss associated with X-linked retinitis pigmentosa (XLRP); the DHAX Trial. We previously reported no significant differences between supplemented and placebo groups in intent-to-tr...

journal_title：Investigative ophthalmology & visual science

authors： Hoffman DR,Hughbanks-Wheaton DK,Spencer R,Fish GE,Pearson NS,Wang YZ,Klein M,Takacs A,Locke KG,Birch DG

更新日期：2015-10-01 00:00:00

abstract：PURPOSE:To compare the prophylactic effects of intravenous moxifloxacin and vancomycin for Staphylococcus epidermidis endophthalmitis in a rabbit model. METHODS:Albino rabbits (n = 60) were divided into three groups. Intravenous moxifloxacin was injected into 20 animals (group 1), and intravenous vancomycin was inject...

journal_title：Investigative ophthalmology & visual science

authors： Kim JY,Kim SY

更新日期：2011-03-25 00:00:00