Molecular cloning, membrane topology, and localization of bovine rom-1 in rod and cone photoreceptor cells.


PURPOSE:To characterize the molecular properties, cellular distribution, and subcellular distribution of bovine rom-1 and its interaction with peripherin/rds in photoreceptor cells as an important step toward elucidating the role of rom-1 in photoreceptor outer segment structure-function relationships and in inherited retinal degenerative disorders. METHODS:Bovine rom-1 cDNA, including a portion of the promoter region, was cloned, sequenced, and heterologously expressed in CHO-K1 and COS-1 cultured mammalian cells. Monoclonal and polyclonal antibodies to specific regions of bovine rom-1 were generated and used with biochemical and immunocytochemical techniques to study the membrane topology, subcellular distribution, and interaction of rom-1 with peripherin/rds. RESULTS:The primary structure of bovine rom-1 is highly homologous to that of human and mouse rom-1. Proteolytic digestion studies and immunolabeling studies of rom-1 in rod outer segment membranes indicate that the C-terminus of rom-1 is localized to the cytoplasmic side and that a large segment is localized to the lumen side of the disc membrane. Post-embedding and pre-embedding immunogold labeling studies for electron microscopy show that rom-1 is localized to the rim region of bovine rod outer segment disc membranes; rom-1 or a closely related homologue also is present at the rim region of cone outer segment disc membranes. Immunofluorescence studies of mammalian cells expressing rom-1 indicate that rom-1 is not translocated to the plasma membrane but instead is retained in internal cellular membranes. Immunoprecipitation studies indicate that all rom-1 and peripherin/rds from rod outer segments form a tightly associated complex. CONCLUSIONS:Rom-1 and peripherin/rds are two structurally related protein subunits of an integral membrane protein complex found on the rim region of rod outer segment disc membranes. Rom-1 or a homologue also is present in the rim region of cone outer segment disc membranes, where it most likely associates with peripherin/rds to form a membrane protein complex.


Moritz OL,Molday RS


Has Abstract


1996-02-01 00:00:00












  • Clinicopathologic effects of the Q64ter rhodopsin mutation in retinitis pigmentosa.

    abstract:PURPOSE:To correlate retinal histopathology with functional changes caused by the rhodopsin Q64ter mutation. METHODS:A 50-year-old female heterozygote was evaluated clinically and with psychophysical and electroretinographic measurements of rod and cone function. The retinas obtained after death were examined microsco...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Milam AH,Li ZY,Cideciyan AV,Jacobson SG

    更新日期:1996-04-01 00:00:00

  • Development of Transparent Acellular Dermal Matrix as Tissue-Engineered Stroma Substitute for Central Lamellar Keratoplasty.

    abstract:Purpose:To improve the transparency of the acellular dermal matrix (ADM) and investigate the optical, mechanical and histologic properties and biocompatibility of transparent ADM (TADM) in lamellar keratoplasty. Methods:A stepwise sectioning strategy was applied to determine the transparency distribution of the ADM, a...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Wang Y,Ma J,Jiang X,Liu Z,Yang J,Li X

    更新日期:2020-01-23 00:00:00

  • Relaxation of beta-structure in tear lipocalin and enhancement of retinoid binding.

    abstract:PURPOSE:To study binding of retinoids to human tear lipocalin (TL) to assess factors influencing ligand affinity and delivery. Mechanistic features of retinoid interactions with TL were investigated, including the influence of the retinoid functional group on ligand affinity, the relative affinity of retinol versus fat...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Gasymov OK,Abduragimov AR,Yusifov TN,Glasgow BJ

    更新日期:2002-10-01 00:00:00

  • Coordinated genetic scaling of the human eye: shared determination of axial eye length and corneal curvature.

    abstract:PURPOSE:To examine the extent to which the two major determinants of refractive error, corneal curvature and axial length, are scaled relative to one another by shared genetic variants, along with their relationship to the genetic scaling of height. METHODS:Corneal curvature, axial length, and height were measured in ...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Guggenheim JA,Zhou X,Evans DM,Timpson NJ,McMahon G,Kemp JP,St Pourcain B,Northstone K,Ring SM,Fan Q,Wong TY,Cheng CY,Khor CC,Aung T,Saw SM,Williams C

    更新日期:2013-03-07 00:00:00

  • Extracellular matrix gene alternative splicing by trabecular meshwork cells in response to mechanical stretching.

    abstract:PURPOSE:Elevated intraocular pressure (IOP), sensed as mechanical stretching by trabecular meshwork (TM) cells, triggers extracellular matrix (ECM) remodeling. In addition to changes in gene expression, alternative mRNA splicing may alter ECM protein isoforms. Changes in mRNA expression and alternative splicing of four...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Keller KE,Kelley MJ,Acott TS

    更新日期:2007-03-01 00:00:00

  • In vivo downregulation of innate and adaptive immune responses in corneal allograft rejection by HC-HA/PTX3 complex purified from amniotic membrane.

    abstract:PURPOSE:Heavy chain-hyaluronic acid (HC-HA)/PTX3 purified from human amniotic membrane (AM) was previously observed to suppress inflammatory responses in vitro. We now examine whether HC-HA/PTX3 is able to exert a similar effect in vivo, using murine models for keratitis and corneal allograft rejection. METHODS:The in...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: He H,Tan Y,Duffort S,Perez VL,Tseng SC

    更新日期:2014-03-19 00:00:00

  • Abundant expression of ponsin, a focal adhesion protein, in lens and downregulation of its expression by impaired cytoskeletal signaling.

    abstract:PURPOSE:This study was undertaken to improve understanding of the defective lens developmental changes induced by the transgenic overexpression of the Rho GDP dissociation inhibitor RhoGDIalpha. The study was focused on a single differentially expressed gene encoding ponsin, a cell adhesion interacting signaling adapto...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Rao PV,Maddala R

    更新日期:2009-04-01 00:00:00

  • Microarray characterization of human embryonic stem cell--derived retinal cultures.

    abstract:PURPOSE:A number of protocols have been published to induce retinal determination from human embryonic stem cells (hESC) and induced pluripotent stem cells (iPSC). Although all these studies have shown some degree of expression of markers of retinal cells, fewer than 30 markers are typically used to characterize the ES...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Lamba DA,Reh TA

    更新日期:2011-07-01 00:00:00

  • Loss of inner retinal neurons after retinal ischemia in rats.

    abstract:PURPOSE:Ischemia is a risk factor for eye diseases like ocular vein occlusion or glaucoma. To investigate effects of ischemia-reperfusion (I/R) a lot of different animal models are used, studying one or two different cell types, which creates heterogeneity of data. The aim of this study was to investigate the function ...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Schmid H,Renner M,Dick HB,Joachim SC

    更新日期:2014-04-28 00:00:00

  • Vulnerability of Dopaminergic Amacrine Cells to Chronic Ischemia in a Mouse Model of Oxygen-Induced Retinopathy.

    abstract:PURPOSE:Retinal dopamine deficiency is a potential cause of myopia and visual deficits in retinopathy of prematurity (ROP). We investigated the cellular mechanisms responsible for lowered levels of retinal dopamine in an oxygen-induced retinopathy (OIR) mouse model of ROP. METHODS:Retinopathy was induced by exposing m...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Spix NJ,Liu LL,Zhang Z,Hohlbein JP,Prigge CL,Chintala S,Ribelayga CP,Zhang DQ

    更新日期:2016-06-01 00:00:00

  • Corneal Potential Maps Measured With Multi-Electrode Electroretinography in Rat Eyes With Experimental Lesions.

    abstract:Purpose:Conventional full-field flash electroretinography (ERG) yields a single response waveform that can be useful in the early detection and diagnosis of many diseases affecting the retina. It is an objective measurement that probes the entire retina. However, localized areas of dysfunction have relatively small inf...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Derafshi Z,Kunzer BE,Mugler EM,Rokhmanova N,Park DW,Tajalli H,Shetty K,Ma Z,Williams JC,Hetling JR

    更新日期:2017-06-01 00:00:00

  • An assessment of polymorphonuclear leukocyte rigidity in HIV-infected individuals after immune recovery.

    abstract:PURPOSE:To determine whether polymorphonuclear leukocytes (PMNs) remain rigid after immune reconstitution in human immunodeficiency virus (HIV)-infected individuals with a history of severe immunosuppression. METHODS:PMN rigidity was measured in vitro in three groups: (1) HIV-infected individuals with a history of CD4...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Goldenberg DT,Holland GN,Cumberland WG,Fisher TC,Folz IC,Wang RC,Terry BG,Moe AA,Kramer F,Lim JI,Rao NA,Meiselman HJ

    更新日期:2002-06-01 00:00:00

  • Klf4 regulates the expression of Slurp1, which functions as an immunomodulatory peptide in the mouse cornea.

    abstract:PURPOSE:The secreted Ly6/uPAR-related protein-1 (Slurp1), associated with the hyperkeratotic disorder mal de Meleda, is abundantly expressed in corneas. Here, we examine its corneal expression and functions. METHODS:Gene expression was quantified by quantitative PCR (qPCR), immunoblots, and immunofluorescent staining....

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Swamynathan S,Buela KA,Kinchington P,Lathrop KL,Misawa H,Hendricks RL,Swamynathan SK

    更新日期:2012-12-19 00:00:00

  • Three-dimensional reconstruction of extravascular matrix patterns and blood vessels in human uveal melanoma tissue: techniques and preliminary findings.

    abstract:PURPOSE:Looping patterns rich in laminin are present in tissue samples of primary aggressive human uveal melanomas and their metastases. Because these extravascular patterns connect to blood vessels and transmit fluid in vitro and in vivo, the three-dimensional configuration of these patterns has been the subject of co...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Chen X,Ai Z,Rasmussen M,Bajcsy P,Auvil L,Welge M,Leach L,Vangveeravong S,Maniotis AJ,Folberg R

    更新日期:2003-07-01 00:00:00

  • ITE, a novel endogenous nontoxic aryl hydrocarbon receptor ligand, efficiently suppresses EAU and T-cell-mediated immunity.

    abstract:PURPOSE:Ligands for aryl hydrocarbon receptor (AHR), such as dioxins, are highly toxic. One such ligand, TCDD, was found to exert potent immunosuppressive capacities in mice developing pathogenic autoimmune processes, including EAU, but its toxicity makes it unusable for humans. A recently identified endogenous AHR lig...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Nugent LF,Shi G,Vistica BP,Ogbeifun O,Hinshaw SJ,Gery I

    更新日期:2013-11-13 00:00:00

  • Detection efficiency of circular gratings and bandpass filtered points with randomized phase spectra.

    abstract:PURPOSE:In the studies of spatial integration an increase in the spatial extent of the stimulus usually results in a decrease in the spatial frequency bandwidth of the stimulus. The authors investigated separately the effects of these two factors on contrast detectability. METHODS:Efficiencies were measured for a circ...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Kukkonen H,Näsänen R,Rovamo J

    更新日期:1994-06-01 00:00:00

  • IL-4 and IL-13 regulation of ICAM-1 expression and eosinophil recruitment in Onchocerca volvulus keratitis.

    abstract:PURPOSE:The presence of eosinophilic granulocytes in ocular tissue is a hallmark of the host response to environmental and parasite allergens. Using a mouse model of Onchocerca volvulus-mediated keratitis (river blindness), the present study examined the role of the cytokines interleukin (IL)-4 and IL-13 in regulating ...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Berger RB,Blackwell NM,Lass JH,Diaconu E,Pearlman E

    更新日期:2002-09-01 00:00:00

  • Analytic subcellular fractionation of acini from rat lacrimal gland.

    abstract::A recent hypothesis for the cellular mechanism of fluid secretion by lacrimal acini has been based, in part, on the results of subcellular fractionation analyses of lacrimal gland fragments which had been incubated for a brief period in vitro. An important assumption in those studies was that the ion transporters and ...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Yiu SC,Wood RL,Mircheff AK

    更新日期:1990-11-01 00:00:00

  • Detection of the bcl-2 t(14;18) translocation and proto-oncogene expression in primary intraocular lymphoma.

    abstract:PURPOSE:Primary intraocular lymphoma (PIOL) is a diffuse large B cell lymphoma that initially infiltrates the retina, vitreous, or optic nerve head, with or without central nervous system involvement. This study examined the expression of the bcl-2 t(14;18) translocation, the bcl-10 gene, and high expression of bcl-6 m...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Wallace DJ,Shen D,Reed GF,Miyanaga M,Mochizuki M,Sen HN,Dahr SS,Buggage RR,Nussenblatt RB,Chan CC

    更新日期:2006-07-01 00:00:00

  • Enzymatic breakdown of type II collagen in the human vitreous.

    abstract:PURPOSE:To investigate whether enzymatic collagen breakdown is an active process in the human vitreous. METHODS:Human donor eyes were used for immunohistochemistry to detect the possible presence of the matrix metalloproteinase (MMP)-induced type II collagen breakdown product col2-3/4C-short in the vitreous. Western b...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: van Deemter M,Pas HH,Kuijer R,van der Worp RJ,Hooymans JM,Los LI

    更新日期:2009-10-01 00:00:00

  • Glycogen and DNA content of corneal epithelium: comparison of preparation methods.

    abstract::Three methods of corneal epithelial preparation for subsequent analysis of epithelial glycogen and DNA content were compared: scraping, lamellar stripping, and in situ freezing. All three methods gave good recovery of these substances. However, there was incomplete recovery of the sample using the in situ freezing met...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Friend J,Kiorpes T,Kinoshita S

    更新日期:1983-02-01 00:00:00

  • Accommodative performance of children with unilateral amblyopia.

    abstract:PURPOSE:The purpose of this study was to compare the accommodative performance of the amblyopic eye of children with unilateral amblyopia to that of their nonamblyopic eye, and also to that of children without amblyopia, during both monocular and binocular viewing. METHODS:Modified Nott retinoscopy was used to measure...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Manh V,Chen AM,Tarczy-Hornoch K,Cotter SA,Candy TR

    更新日期:2015-01-27 00:00:00

  • Saccade adaptation in Williams-Beuren Syndrome.

    abstract:PURPOSE:To investigate the capacity for rapid saccade adaptation in Williams-Beuren Syndrome (WBS), a genetic neurodevelopmental disorder, in which it has been observed that saccadic accuracy is severely reduced. METHODS:Saccade amplitude modification was elicited by backward steps (30% of target eccentricity) during ...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: van der Geest JN,Haselen GC,Frens MA

    更新日期:2006-04-01 00:00:00

  • Gene expression networks underlying retinoic acid-induced differentiation of human retinoblastoma cells.

    abstract:PURPOSE:To understand the genetic regulatory pathways underlying the retinoic acid (RA) induction of cone arrestin, gene array technology and other molecular tools were used to profile global gene expression changes in human retinoblastoma cells. METHODS:Weri-Rb-1 retinoblastoma cells were cultured in the absence or p...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Li A,Zhu X,Brown B,Craft CM

    更新日期:2003-03-01 00:00:00

  • Attenuated glial reaction in experimental proliferative vitreoretinopathy treated with liposomal doxorubicin.

    abstract:PURPOSE:We investigated the therapeutic effect of liposomal doxorubicin (Lipo-dox) on experimental proliferative vitreoretinopathy (PVR). METHODS:The toxicity of Lipo-dox was determined in vitro in cultured rabbit retinal pigment epithelium (RPE) cells by tetrazolium-based (MTT) assay for cell viability performed 48 a...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Kuo HK,Chen YH,Wu PC,Wu YC,Huang F,Kuo CW,Lo LH,Shiea J

    更新日期:2012-06-29 00:00:00

  • Lenses of diabetic patients "yellow" at an accelerated rate similar to older normals.

    abstract::The authors used a psychophysical method to measure lens transmission of young, type I diabetic patients and normal controls. The results from normal controls agreed with previously published reports of decreasing lens transmission with age, and those from diabetic subjects suggested that lenses of young, type I diabe...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Lutze M,Bresnick GH

    更新日期:1991-01-01 00:00:00

  • Angioscotometry with the scanning laser ophthalmoscope. Comparison of the effect of different wavelengths.

    abstract:PURPOSE:Angioscotomas are scotomata caused by vessel shadows. Their extent may be influenced by physiological and pharmacologic conditions and disease. In this study, the authors quantified angioscotomas in normal subjects using a fundus perimetry technique with a scanning laser ophthalmoscope. They further investigate...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Remky A,Beausencourt E,Elsner AE

    更新日期:1996-10-01 00:00:00

  • The inheritance of peripapillary atrophy.

    abstract:PURPOSE:To estimate the relative importance of genes and environment in peripapillary atrophy type beta (beta-PPA) in a classic twin study. METHODS:Female twin pairs (n = 506) aged 49 to 79 years were recruited from the St. Thomas' UK Adult Twin Registry. Peripapillary atrophy was identified from masked grading of ste...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Healey PR,Mitchell P,Gilbert CE,Lee AJ,Ge D,Snieder H,Spector TD,Hammond CJ

    更新日期:2007-06-01 00:00:00

  • Regulation of choroidal blood flow during combined changes in intraocular pressure and arterial blood pressure.

    abstract:PURPOSE:To test the hypothesis that human choroidal blood flow (ChBF) may depend, not only on ocular perfusion pressure (OPP), but also on absolute mean arterial pressure (MAP) and intraocular pressure (IOP). METHODS:There were two study days in an open design. On the first day, OPP was varied by elevating IOP during ...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Polska E,Simader C,Weigert G,Doelemeyer A,Kolodjaschna J,Scharmann O,Schmetterer L

    更新日期:2007-08-01 00:00:00

  • Age and topographic variation of insulin-like growth factor-binding protein 2 in the human rpe.

    abstract:PURPOSE:Previous studies have shown that insulin-like growth factor-binding protein (IGFBP)-2 is markedly upregulated in senescent RPE cells in vitro, and might therefore be a marker of senescent cells in vivo. This study was conducted to determine whether IGFBP-2 expression in human RPE cells from the macula and perip...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Miyamura N,Mishima K,Honda S,Aotaki-Keen AE,Morse LS,Handa JT,Hjelmeland LM

    更新日期:2001-06-01 00:00:00