Abstract:
PURPOSE:Diabetic retinopathy (DR) has features of chronic low-grade inflammation. The purpose of our study was to investigate whether the presence of inflammatory cells in fibrovascular membranes (FVMs) from patients with proliferative diabetic retinopathy (PDR) is associated with the activity of PDR and visual acuity improvement after vitreoretinal surgery. METHODS:Forty FVMs from 40 patients with PDR were obtained during vitrectomy, prepared by using the agar sandwich method, and examined using light microscope and immunohistochemistry methods to define the presence and density of inflammatory cells: CD45+ cells (leukocytes), CD4+ cells (T helper lymphocytes), CD8+ cells (T cytotoxic lymphocytes), CD19+ cells (B lymphocytes), and CD14+ cells (monocytes/macrophages). For each FVM, the inflammatory cell density defined as numerical areal density was calculated. The number of vessels was defined as the volume density of vessels. RESULTS:Among 40 patients with PDR, 33 patients had active PDR and 7 quiescent PDR. Significant differences in cell densities for CD4+, CD8+, and CD19+ cells were observed between patients with active and quiescent PDR. B lymphocytes were present in membranes of active PDR only. No correlation was observed between numerical areal density of inflammatory cells and the volume density of vessels. No association was found between visual acuity improvement after surgery and cell densities. CONCLUSIONS:Lymphocyte infiltration of FVMs might be associated with the activity of retinopathy but not with visual acuity improvement after surgery.
journal_name
Invest Ophthalmol Vis Scijournal_title
Investigative ophthalmology & visual scienceauthors
Urbančič M,Štunf Š,Milutinović Živin A,Petrovič D,GlobočnikPetrovič Mdoi
10.1167/iovs.13-13634subject
Has Abstractpub_date
2014-11-25 00:00:00pages
8576-82issue
12eissn
0146-0404issn
1552-5783pii
iovs.13-13634journal_volume
55pub_type
杂志文章abstract::Visual fixation of a small, stationary target was measured in 12 young observers (mean age = 22 yr) and in 12 older observers (mean age = 70 yr). The two groups' fixation behavior did not differ on various dimensions of fixation stability: mean fixation area, intra-subject variability, or changes in fixation over succ...
journal_title:Investigative ophthalmology & visual science
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doi:
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journal_title:Investigative ophthalmology & visual science
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journal_title:Investigative ophthalmology & visual science
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doi:
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journal_title:Investigative ophthalmology & visual science
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journal_title:Investigative ophthalmology & visual science
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journal_title:Investigative ophthalmology & visual science
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