Abstract:
:Cytokeratin antibodies have been widely used for the identification of trophoblast cells in the placental bed, following their invasion from the developing conceptus. Their identification centres upon the expression of cytokeratin in epithelial cells, from which trophoblast cells are derived. Our recent observations indicate that this strict relationship may be more complex than was thought. Cryostat and paraffin sections of human decidua and myometrium, taken from the placental bed and the uterotomy cut, were examined immunocytochemically for cytokeratins using ten antibody clones selected to identify different cytokeratin proteins and antigenic epitopes. Biopsy specimens were obtained from normal and pathological pregnancies (pre-eclampsia, fetal retardation, amnioninfection, hysterorrhexis, placenta praevia) at the time of caesarean section (26-41 weeks of pregnancy). Antibodies against nine clones, CAM 5.2, MNF 116, AE1/AE3, CK5, KS-B17.2, CY-90, M20, E3, and 34 beta E12 identified, as expected, syncytial giant cells and mononuclear trophoblasts within the placental bed and glandular epithelial cells throughout the uterus. In addition, they stained numerous fusiform cells that were classified by established criteria to represent smooth muscle cells, both within blood vessels and myometrium. No staining differences were observed between normal and pathological disorders. These results indicate that cytokeratin antibodies CAM 5.2, MNF 116 and AE1/AE3, and other antibodies targeting proteins 8 and 18, cross-react with epitopes expressed in cells other than giant trophoblastic cells and mononuclear trophoblasts in the uterus and, thus, caution has to be used when such antibodies are used for the diagnostic characterization of tissues related to the placental bed.
journal_name
Histopathologyjournal_title
Histopathologyauthors
Stiemer B,Graf R,Neudeck H,Hildebrandt R,Hopp H,Weitzel HKdoi
10.1111/j.1365-2559.1995.tb00303.xsubject
Has Abstractpub_date
1995-11-01 00:00:00pages
407-14issue
5eissn
0309-0167issn
1365-2559journal_volume
27pub_type
杂志文章相关文献
HISTOPATHOLOGY文献大全abstract:AIMS:To identify clinicopathological characteristics of cholangiocarcinoma and premalignant lesions arising in patients with primary sclerosing cholangitis (PSC). METHODS AND RESULTS:This study consisted of 25 patients with PSC and bile duct neoplasia [16 with cholangiocarcinoma and nine with biliary intra-epithelial ...
journal_title:Histopathology
pub_type: 杂志文章
doi:10.1111/j.1365-2559.2011.04048.x
更新日期:2011-12-01 00:00:00
abstract:AIM:To train and individually validate a group of breast pathologists in specialty-specific digital primary diagnosis by using a novel protocol endorsed by the Royal College of Pathologists' new guideline for digital pathology. The protocol allows early exposure to live digital reporting, in a risk-mitigated environmen...
journal_title:Histopathology
pub_type: 杂志文章
doi:10.1111/his.13403
更新日期:2018-03-01 00:00:00
abstract::There are many significant differences, but also considerable overlap between the quantitative histopathological features of mild and marked atypical endometrial hyperplasias and well and moderately differentiated carcinomas, thus preventing its application to individual patient care. To try to overcome this problem, ...
journal_title:Histopathology
pub_type: 杂志文章
doi:10.1111/j.1365-2559.1981.tb01835.x
更新日期:1981-11-01 00:00:00
abstract:AIMS:Secretory carcinoma (SC) is a rare histological type of breast cancer, and ETV6-NTRK3 gene fusion is highly specific to it. The differential diagnoses of SC include acinic cell carcinoma (ACCA) and cystic hypersecretory carcinoma (CHC), as well as invasive ductal carcinoma (IDC). For patients with these rare but d...
journal_title:Histopathology
pub_type: 杂志文章
doi:10.1111/his.12172
更新日期:2013-10-01 00:00:00
abstract:AIMS:Prostate gland volume is generally assessed by transrectal ultrasound examination or magnetic resonance imaging at the time of prostatic biopsy or pre-brachytherapy planning. The volume is most commonly measured using the formula, prostate volume = height x width x length x pi/6, which is derived considering the g...
journal_title:Histopathology
pub_type: 杂志文章
doi:10.1111/j.1365-2559.2010.03591.x
更新日期:2010-07-01 00:00:00
abstract:AIMS:To analyse the possible activation of distinct molecular pathways in mucosa-associated lymphoid tissue (MALT) lymphoma, we determined the prevalence of trisomies 3, 12, 18 in MALT lymphomas from different organs, as well as the prevalence of translocations of the MALT1 gene in a subset of primary breast MALT lymph...
journal_title:Histopathology
pub_type: 杂志文章
doi:10.1111/j.1365-2559.2007.02580.x
更新日期:2007-01-01 00:00:00
abstract:AIMS:Gallbladder cancer (GBC) is an aggressive tumour that is usually diagnosed at advanced stages and is characterised by a poor prognosis. Using public data of normal human tissues, we found that mRNA and protein levels of mucin 5B (MUC5B) and carbonic anhydrase 9 (CA9) were highly increased in gallbladder tissues. I...
journal_title:Histopathology
pub_type: 杂志文章
doi:10.1111/his.13797
更新日期:2019-03-01 00:00:00
abstract:AIMS:The aims of this study were to define whether diffuse cutaneous reticulohistiocytosis could be underpinned by somatic genetic alterations and represent a precursor of more aggressive forms of disease. METHODS AND RESULTS:A 59-year-old man with diffuse cutaneous reticulohistiocytosis experienced bone marrow locali...
journal_title:Histopathology
pub_type:
doi:10.1111/his.13166
更新日期:2017-05-01 00:00:00
abstract::In recent years, there have been several important refinements in the classification of cutaneous mesenchymal neoplasms, including the description of new tumour types, along with the identification of novel and recurrent molecular genetic findings. In addition to providing new insights into tumour biology, many of the...
journal_title:Histopathology
pub_type: 杂志文章,评审
doi:10.1111/his.12930
更新日期:2016-05-01 00:00:00
abstract:AIMS:The presence of micrometastases in the sentinel lymph node (SLN) is an important prognostic parameter for melanoma patients. The aim was to determine the prognostic relevance of histopathological characteristics of micrometastases in the SLN, which has not been adequately addressed thus far. METHODS AND RESULTS:I...
journal_title:Histopathology
pub_type: 杂志文章
doi:10.1111/j.1365-2559.2007.02681.x
更新日期:2007-05-01 00:00:00
abstract:AIMS:Tumour budding is an adverse prognostic factor in colorectal cancer (CRC). We have investigated the significance of cytoplasmic fragments occurring in the immediate vicinity of tumour budding foci. METHODS AND RESULTS:Seventy-three CRCs with high-grade budding (> 10 budding foci in a x 20 objective field) were cl...
journal_title:Histopathology
pub_type: 杂志文章
doi:10.1111/j.1365-2559.2005.02162.x
更新日期:2005-07-01 00:00:00
abstract:AIMS:Richter transformation (RT) occurs in 5-10% of patients with chronic lymphocytic leukaemia/small lymphocytic lymphoma (CLL/SLL), and is associated with a poor outcome. The histological manifestations of RT are variable, and include an increase in the proportion of prolymphocytes, immunoblasts, and other pleomorphi...
journal_title:Histopathology
pub_type: 杂志文章,评审
doi:10.1111/his.13137
更新日期:2017-04-01 00:00:00
abstract::Fifteen hamartomas of the breast have been studied by dissecting microscopy of thick sections at a depth of 1.0 to 2.5 mm, and by conventional 5 microns deep sections. This combined approach has revealed six distinctive features, three in the parenchymal structure and three in the connective tissue components. Ducts (...
journal_title:Histopathology
pub_type: 杂志文章
doi:10.1111/j.1365-2559.1994.tb01296.x
更新日期:1994-02-01 00:00:00
abstract::Current treatment of breast cancer incorporates clinical, pathological and molecular data. Oestrogen receptor (ER), progesterone receptor (PR) and human epidermal growth factor receptor 2 (HER2) define prognosis and identify tumours for targeted therapy, and remain the sole established single-molecule biomarkers defin...
journal_title:Histopathology
pub_type: 杂志文章,评审
doi:10.1111/his.12795
更新日期:2016-01-01 00:00:00
abstract:AIMS:Because of its efficacy, oxaliplatin (OX) is increasingly used as a chemotherapeutic agent in the treatment of colorectal liver metastases (CRLM). Oxaliplatin-associated liver toxicity has been reported and can affect clinical practice, but studies on its prevalence and a full pathological description are lacking....
journal_title:Histopathology
pub_type: 杂志文章
doi:10.1111/j.1365-2559.2010.03511.x
更新日期:2010-03-01 00:00:00
abstract::The assessment of squamous lesions of the uterine cervix is a major preoccupation of diagnostic histopathologists and this can overshadow the recognition of significant abnormalities affecting the adjacent endocervical glands. This review gives an account of recent advances in our knowledge of the histology and histoc...
journal_title:Histopathology
pub_type: 杂志文章
doi:10.1111/j.1365-2559.1986.tb02578.x
更新日期:1986-08-01 00:00:00
abstract:AIMS:The preoperative differentiation of malignant renal cystic tumours from benign lesions is critical, and it remains a common diagnostic problem. The aim was to examine if the Carbonic anhydrase 9 (CA9) level in cyst fluid can provide a molecular diagnosis of malignant cyst. METHODS AND RESULTS:Twenty-eight patient...
journal_title:Histopathology
pub_type: 杂志文章
doi:10.1111/j.1365-2559.2009.03302.x
更新日期:2009-06-01 00:00:00
abstract:AIMS:The diagnosis of polymorphous low-grade adenocarcinoma (PLGA) remains difficult for general pathologists, particularly in cases of small biopsy samples. We aimed to characterize the histopathological spectrum and immunohistochemical aspects by using an accessible immunohistochemical panel of cytoskeletal proteins ...
journal_title:Histopathology
pub_type: 杂志文章
doi:10.1111/his.12880
更新日期:2016-06-01 00:00:00
abstract::A recently developed and validated histochemical method--immunogold-streptavidin enhancement--was used to determine oestrogen receptor content in paraffin sections of breast cancers. The selection of the best cut-off point to define oestrogen receptor status as rich or poor was made on the basis of survival data, usin...
journal_title:Histopathology
pub_type: 杂志文章
doi:10.1111/j.1365-2559.1990.tb00735.x
更新日期:1990-10-01 00:00:00
abstract::Several malignant tumours occurring in the sinonasal tract may present with an undifferentiated morphology. Overall, these lesions pose significant diagnostic difficulties for the surgical pathologist, especially in limited biopsy material, but their correct classification is becoming increasingly important for an app...
journal_title:Histopathology
pub_type: 杂志文章,评审
doi:10.1111/j.1365-2559.2011.03813.x
更新日期:2011-12-01 00:00:00
abstract:AIMS:Mucinous tubular and spindle cell carcinoma (MTSC) of the kidney is a distinct entity characterized by bland tightly packed elongated tubules and spindle cells with low nucleolar grade in a basophilic mucinous stroma. Several case studies have reported MTSC with high-grade features and have brought into question w...
journal_title:Histopathology
pub_type: 杂志文章
doi:10.1111/his.13298
更新日期:2017-11-01 00:00:00
abstract:AIMS:To document the clinical, light microscopic, immunohistochemical and ultrastructural features of four cases of extra-orbital giant cell angiofibromas. METHODS AND RESULTS:Sections of formalin-fixed paraffin-embedded specimens were studied by haematoxylin and eosin, reticulin and immunohistochemical stains. Electr...
journal_title:Histopathology
pub_type: 杂志文章,评审
doi:10.1046/j.1365-2559.2001.01227.x
更新日期:2001-10-01 00:00:00
abstract::Cancer biomarkers provide an opportunity to diagnose tumours earlier and with greater accuracy. They can also identify those patients most at risk of disease recurrence and predict which tumours will respond to different therapeutic approaches. Such biomarkers will be especially useful in the diagnosis and management ...
journal_title:Histopathology
pub_type: 杂志文章,评审
doi:10.1111/j.1365-2559.2010.03592.x
更新日期:2010-07-01 00:00:00
abstract:AIMS:This study aimed to assess the feasibility of using virtual slides to create 3D histopathological reconstructions to aid in the study of the biology of DCIS. METHODS:Four μm thick serial sections of formalin fixed paraffin embedded tissue from three cases were cut and mounted onto glass slides, stained with haema...
journal_title:Histopathology
pub_type: 杂志文章
doi:10.1111/his.12561
更新日期:2015-06-01 00:00:00
abstract::Some antigenic determinants can be preserved in tissues after plastic embedding. In the present study liver tissue was fixed with glutaraldehyde or paraformaldehyde, dehydrated with ethanol and toluene and embedded in araldite which was then polymerized at 37 degrees C. Immunofluorescence was performed on semi-thin se...
journal_title:Histopathology
pub_type: 杂志文章
doi:10.1111/j.1365-2559.1977.tb01674.x
更新日期:1977-09-01 00:00:00
abstract:AIMS:Glutamate receptor antagonists have failed clinical stroke trials and it has been proposed that the action of N-methyl D-aspartate receptors is necessary for neuronal survival. Thus, excitatory amino acid transporters (EAATs) might be a promising therapeutic target. The aim of this study was to investigate glial e...
journal_title:Histopathology
pub_type: 杂志文章
doi:10.1111/j.1365-2559.2007.02703.x
更新日期:2007-06-01 00:00:00
abstract:AIMS:CD30 immunohistochemistry (IHC) in malignant lymphoma is used for selection of patients in clinical trials using brentuximab vedotin, an antibody drug-conjugate targeting the CD30 molecule. For reliable implementation in daily practice and meaningful selection of patients for clinical trials, information on techni...
journal_title:Histopathology
pub_type: 杂志文章
doi:10.1111/his.13647
更新日期:2018-09-01 00:00:00
abstract::Two cases of tubulovillous adenoma and adjacent colonic mucosa were studied in which intestinal spirochaetosis had heavily colonized the non-neoplastic but not the neoplastic epithelium. Ultrastructural and histochemical studies were performed and we postulate that the reason for this disparity is the normal microvill...
journal_title:Histopathology
pub_type: 杂志文章
doi:10.1111/j.1365-2559.1995.tb01530.x
更新日期:1995-10-01 00:00:00
abstract::The ultrastructure of stromal mononuclear cells and giant cells were studied in two cases of central giant cell granuloma of the jaws. The majority of stromal cells superficially resembled fibroblasts, but also contained intracytoplasmic myofilaments with electron dense bodies similar to smooth muscle cells. These cel...
journal_title:Histopathology
pub_type: 杂志文章
doi:10.1111/j.1365-2559.1983.tb02305.x
更新日期:1983-11-01 00:00:00
abstract:AIMS:Colonic and rectal biopsies, often taken from an endoscopically normal large bowel, form a significant proportion of the histopathology workload. The aim of this study was to determine diagnostic yield from mucosal biopsies in patients with normal colonoscopy or sigmoidoscopy, and whether or not diarrhoea is predi...
journal_title:Histopathology
pub_type: 杂志文章
doi:10.1111/j.1365-2559.2012.04317.x
更新日期:2012-12-01 00:00:00