Antibodies to cytokeratins bind to epitopes in human uterine smooth muscle cells in normal and pathological pregnancies.


:Cytokeratin antibodies have been widely used for the identification of trophoblast cells in the placental bed, following their invasion from the developing conceptus. Their identification centres upon the expression of cytokeratin in epithelial cells, from which trophoblast cells are derived. Our recent observations indicate that this strict relationship may be more complex than was thought. Cryostat and paraffin sections of human decidua and myometrium, taken from the placental bed and the uterotomy cut, were examined immunocytochemically for cytokeratins using ten antibody clones selected to identify different cytokeratin proteins and antigenic epitopes. Biopsy specimens were obtained from normal and pathological pregnancies (pre-eclampsia, fetal retardation, amnioninfection, hysterorrhexis, placenta praevia) at the time of caesarean section (26-41 weeks of pregnancy). Antibodies against nine clones, CAM 5.2, MNF 116, AE1/AE3, CK5, KS-B17.2, CY-90, M20, E3, and 34 beta E12 identified, as expected, syncytial giant cells and mononuclear trophoblasts within the placental bed and glandular epithelial cells throughout the uterus. In addition, they stained numerous fusiform cells that were classified by established criteria to represent smooth muscle cells, both within blood vessels and myometrium. No staining differences were observed between normal and pathological disorders. These results indicate that cytokeratin antibodies CAM 5.2, MNF 116 and AE1/AE3, and other antibodies targeting proteins 8 and 18, cross-react with epitopes expressed in cells other than giant trophoblastic cells and mononuclear trophoblasts in the uterus and, thus, caution has to be used when such antibodies are used for the diagnostic characterization of tissues related to the placental bed.






Stiemer B,Graf R,Neudeck H,Hildebrandt R,Hopp H,Weitzel HK




Has Abstract


1995-11-01 00:00:00












  • The presence and localization of onychodermis (specialized nail mesenchyme) containing onychofibroblasts in the nail unit: a morphological and immunohistochemical study.

    abstract:AIMS:Due to its limited availability there has been very little research on the mesenchyme of the nail unit. Recently, we discovered specialized mesenchymal cells beneath the nail matrix and proposed to call them onychofibroblasts. The purpose of this study was to further delineate more precisely by histology and immun...


    pub_type: 杂志文章


    authors: Lee DY,Park JH,Shin HT,Yang JM,Jang KT,Kwon GY,Lee KH,Shim JS

    更新日期:2012-07-01 00:00:00

  • Dilated (congestive) cardiomyopathy: a syndrome of severe cardiac dysfunction with remarkably few morphological features of myocardial damage.

    abstract::A review of 76 endomyocardial biopsies and 54 autopsies of patients with dilated cardiomyopathy (DCM) revealed features of hypertrophy and degenerative changes associated with long-standing hypertrophy. Long-term survivors had significantly heavier heart weights. Short-term survivors had heavier than normal right vent...


    pub_type: 杂志文章


    authors: Rose AG,Beck W

    更新日期:1985-04-01 00:00:00

  • BCOR-CCNB3-positive soft tissue sarcoma with round-cell and spindle-cell histology: a series of four cases highlighting the pitfall of mimicking poorly differentiated synovial sarcoma.

    abstract:AIMS:BCOR-CCNB3 sarcoma is a genetically defined undifferentiated malignancy with Ewing sarcoma (ES)-like round cells, and preferentially affects the bones of male adolescents. Sarcomas harbouring BCOR-CCNB3 rarely arise from soft tissues; therefore, we aimed to report four cases to expand the clinicopathological spect...


    pub_type: 杂志文章


    authors: Li WS,Liao IC,Wen MC,Lan HH,Yu SC,Huang HY

    更新日期:2016-11-01 00:00:00

  • Transforming growth factor-beta pathway disruption and infiltration of colorectal cancers by intraepithelial lymphocytes.

    abstract:AIMS:Colorectal cancers deficient in DNA mismatch repair (MMR) are often characterized by the presence of numerous intraepithelial lymphocytes (IELs). These CD8+ T cells selectively express CD103, which is upregulated locally by transforming growth factor (TGF)-beta, and adhere to E-cadherin expressed by mucosal epithe...


    pub_type: 杂志文章


    authors: Baker K,Chong G,Foulkes WD,Jass JR

    更新日期:2006-10-01 00:00:00

  • Advances in the clinicopathological and molecular classification of cutaneous mesenchymal neoplasms.

    abstract::In recent years, there have been several important refinements in the classification of cutaneous mesenchymal neoplasms, including the description of new tumour types, along with the identification of novel and recurrent molecular genetic findings. In addition to providing new insights into tumour biology, many of the...


    pub_type: 杂志文章,评审


    authors: Costigan DC,Doyle LA

    更新日期:2016-05-01 00:00:00

  • Chromosomal imbalances detected in NTRK-rearranged sarcomas by comparative genomic hybridization.

    abstract:AIMS:NTRK gene rearranged sarcomas are emerging as a distinct class of sarcomas of particular importance in the era of targeted therapy. In this study we used Array Comparative Genomic Hybridisation (aCGH) to explore the cytogenetic profile of 6 adult soft tissue sarcomas harbouring NTRK gene fusions. METHODS AND RESU...


    pub_type: 杂志文章


    authors: Vargas AC,Mesbah Ardakani N,Wong DD,Maclean FM,Kattampallil J,Boyle R,Santos L,Gill AJ

    更新日期:2020-10-31 00:00:00

  • Biomarker assessment and molecular testing for prognostication in breast cancer.

    abstract::Current treatment of breast cancer incorporates clinical, pathological and molecular data. Oestrogen receptor (ER), progesterone receptor (PR) and human epidermal growth factor receptor 2 (HER2) define prognosis and identify tumours for targeted therapy, and remain the sole established single-molecule biomarkers defin...


    pub_type: 杂志文章,评审


    authors: Kos Z,Dabbs DJ

    更新日期:2016-01-01 00:00:00

  • Prognostic value of immunohistochemical expression of p53, bax, Bcl-2 and Bcl-xL in resected non-small-cell lung cancers.

    abstract:AIMS:Some experimental evidence suggests that in lung cancer, development, progression and an increased proliferation rate can be linked to apoptosis-related factors. In this study we evaluated the possible role of p53 and Bcl-2 gene family members as prognostic factors for non-small-cell lung cancer. METHODS AND RESU...


    pub_type: 杂志文章


    authors: Groeger AM,Esposito V,De Luca A,Cassandro R,Tonini G,Ambrogi V,Baldi F,Goldfarb R,Mineo TC,Baldi A,Wolner E

    更新日期:2004-01-01 00:00:00

  • Clinicopathological analysis of 46 cases with CD4+ and/or CD56+ immature haematolymphoid malignancy: reappraisal of blastic plasmacytoid dendritic cell and related neoplasms.

    abstract:AIMS:In this study, we aimed to investigate the clinicopathological features of CD4+ and/or CD56+ immature haematolymphoid malignancy (iHLM), including blastic plasmacytoid dendritic cell neoplasm (BPDCN). METHODS AND RESULTS:We analysed the clinicopathological features of 46 patients diagnosed consecutively with CD4+...


    pub_type: 杂志文章


    authors: Suzuki Y,Kato S,Kohno K,Satou A,Eladl AE,Asano N,Kono M,Kato Y,Taniwaki M,Akiyama M,Nakamura S

    更新日期:2017-12-01 00:00:00

  • RBM10-TFE3 renal cell carcinoma characterised by paracentric inversion with consistent closely split signals in break-apart fluorescence in-situ hybridisation: study of 10 cases and a literature review.

    abstract:AIMS:Xp11 rearrangement in renal cell carcinoma (RCC) typically involves gene fusion to the gene encoding transcription factor E3 (TFE3), a member of the microphthalmia-associated transcription factor family on chromosome Xp11.2. Dual-colour break-apart fluorescence in-situ hybridisation (FISH) is recommended to confir...


    pub_type: 杂志文章,评审


    authors: Kato I,Furuya M,Baba M,Kameda Y,Yasuda M,Nishimoto K,Oyama M,Yamasaki T,Ogawa O,Niino H,Nakaigawa N,Yano Y,Sakamoto K,Urata Y,Mikami K,Yamasaki S,Tanaka R,Takagi T,Kondo T,Nagashima Y

    更新日期:2019-08-01 00:00:00

  • Lack of PRKD2 and PRKD3 kinase domain somatic mutations in PRKD1 wild-type classic polymorphous low-grade adenocarcinomas of the salivary gland.

    abstract:AIMS:Polymorphous low-grade adenocarcinoma (PLGA) is the second most common intra-oral salivary gland malignancy. The vast majority of PLGAs harbour a PRKD1 E710D hot-spot somatic mutation or somatic rearrangements of PRKD1, PRKD2 or PRKD3. Given the kinase domain homology among PRKD1, PRKD2 and PRKD3, we sought to def...


    pub_type: 杂志文章


    authors: Piscuoglio S,Fusco N,Ng CK,Martelotto LG,da Cruz Paula A,Katabi N,Rubin BP,Skálová A,Weinreb I,Weigelt B,Reis-Filho JS

    更新日期:2016-06-01 00:00:00

  • MUC4 expression in meningiomas: under-recognized immunophenotype particularly in meningothelial and angiomatous subtypes.

    abstract:AIMS:MUC4 is a transmembrane glycoprotein that plays a role in cell growth signalling and is expressed in various epithelial tissues. Gene expression profiling and immunohistochemical analyses revealed that MUC4 is also constantly and specifically expressed in low-grade fibromyxoid sarcomas and sclerosing epithelioid f...


    pub_type: 杂志文章


    authors: Matsuyama A,Jotatsu M,Uchihashi K,Tsuda Y,Shiba E,Haratake J,Hisaoka M

    更新日期:2019-01-01 00:00:00

  • Expression of activated Notch1 and Hey1 in papillary thyroid carcinoma.

    abstract:AIMS:The Notch signalling pathway is involved in normal development as well as tumorigenesis. However, it is unclear whether Notch activation is related to diverse clinicopathological factors in papillary thyroid carcinoma (PTC). METHODS AND RESULTS:We examined the relationship between clinicopathological factors and ...


    pub_type: 杂志文章


    authors: Jung CW,Kong JS,Seol H,Park S,Koh JS,Lee SS,Kim MJ,Choi IJ,Myung JK

    更新日期:2017-01-01 00:00:00

  • Clinicopathological significance of EGFR pathway gene mutations and CRTC1/3-MAML2 fusions in salivary gland mucoepidermoid carcinoma.

    abstract:AIMS:Mucoepidermoid carcinoma (MEC) is one of the most common salivary gland carcinomas. Epidermal growth factor receptor (EGFR) signalling pathway gene mutations are important in predicting a patient's prognosis, selecting molecularly targeted drugs and estimating the efficacy of a molecular therapy. However, their si...


    pub_type: 杂志文章


    authors: Morita M,Murase T,Okumura Y,Ueda K,Sakamoto Y,Masaki A,Kawakita D,Tada Y,Nibu KI,Shibuya Y,Inagaki H

    更新日期:2020-06-01 00:00:00

  • Primary and recurrent embryonal sarcoma of the liver: clinicopathological and immunohistochemical analysis.

    abstract:AIMS:To evaluate primary and recurrent embryonal sarcoma of the liver and to improve recognition of its morphological variants and immunohistochemical features. METHODS AND RESULTS:Fourteen primary and two recurrent cases of hepatic embryonal sarcoma were evaluated histologically and investigated immunohistochemically...


    pub_type: 杂志文章


    authors: Zheng JM,Tao X,Xu AM,Chen XF,Wu MC,Zhang SH

    更新日期:2007-08-01 00:00:00

  • Interobserver variation in CD30 immunohistochemistry interpretation; consequences for patient selection for targeted treatment.

    abstract:AIMS:CD30 immunohistochemistry (IHC) in malignant lymphoma is used for selection of patients in clinical trials using brentuximab vedotin, an antibody drug-conjugate targeting the CD30 molecule. For reliable implementation in daily practice and meaningful selection of patients for clinical trials, information on techni...


    pub_type: 杂志文章


    authors: Koens L,van de Ven PM,Hijmering NJ,Kersten MJ,Diepstra A,Chamuleau M,de Jong D

    更新日期:2018-09-01 00:00:00

  • Immunohistochemical expression of p53 proteins in Wilms' tumour: a possible association with the histological prognostic parameter of anaplasia.

    abstract::Wilms' tumour (nephroblastoma) has been associated with chromosomal abnormalities at the 11p13, 11p15 and 16q regions. A study into the possibility of mutations occurring within p53, the ubiquitous adult tumour suppressor gene, in Wilms' tumour was carried out. Thirty-eight cases were studied. Of these 36 were categor...


    pub_type: 杂志文章


    authors: Cheah PL,Looi LM,Chan LL

    更新日期:1996-01-01 00:00:00

  • Eosinophilic granuloma of lymph nodes--a variant of histiocytosis X.

    abstract::A clinicopathologic study of histiocytosis X in lymph nodes disclosed a special variant: primary eosinophilic granuloma of lymph nodes. This variant involves one or more lymph nodes, but does not infiltrate any other organs. Histologically, the infiltration of lymph nodes by histiocytosis X cells and eosinophils is si...


    pub_type: 杂志文章


    authors: Motoi M,Helbron D,Kaiserling E,Lennert K

    更新日期:1980-11-01 00:00:00

  • Colonic tubular adenomas and intestinal spirochaetosis: an incompatible association.

    abstract::Two cases of tubulovillous adenoma and adjacent colonic mucosa were studied in which intestinal spirochaetosis had heavily colonized the non-neoplastic but not the neoplastic epithelium. Ultrastructural and histochemical studies were performed and we postulate that the reason for this disparity is the normal microvill...


    pub_type: 杂志文章


    authors: Coyne JD,Curry A,Purnell P,Haboubi NY

    更新日期:1995-10-01 00:00:00

  • What to do with high autofluorescence background in pancreatic tissues - an efficient Sudan black B quenching method for specific immunofluorescence labelling.

    abstract:AIMS:High levels of autofluorescence in tissue samples can entirely mask specific labellings with fluorophores and thus impair immunofluorescence histochemistry. In pancreatic tissue samples we observed autofluorescence as a common problem often mediated by the fixation and processing procedure. METHODS AND RESULTS:Us...


    pub_type: 杂志文章


    authors: Erben T,Ossig R,Naim HY,Schnekenburger J

    更新日期:2016-09-01 00:00:00

  • Possible liver cell differentiation in testicular germ cell tumours.

    abstract::Germ cell tumours may imitate various structures of the developing embryo and foetus. Certain structures have, however, very rarely or never been observed in these tumours. Thus the presence of hepatic tissue in testicular germ cell tumours has not been reported. In a series of 37 non-seminomatous testicular tumours s...


    pub_type: 杂志文章


    authors: Jacobsen GK,Jacobsen M

    更新日期:1983-07-01 00:00:00

  • Adenomatous polyposis coli (APC), beta-catenin, and cadherin are expressed in human bone and cartilage.

    abstract:AIMS:Members of the cadherin and catenin families are involved in chondrogenesis and catenin gene mutations have been detected in malignant tumours of bone. This study was undertaken to assess in detail expression of cadherin, beta-catenin and the associated tumour suppressor gene product APC in bone and cartilage at d...


    pub_type: 杂志文章


    authors: Monaghan H,Bubb VJ,Sirimujalin R,Millward-Sadler SJ,Salter DM

    更新日期:2001-12-01 00:00:00

  • Prognostic significance of histopathological features of extraprostatic extension of prostate cancer.

    abstract:AIMS:The 2009 International Society of Urological Pathology consensus conference recommended reporting the extent of extraprostatic extension (EPE) for the prediction of outcome in prostate cancer. Our aim was to stratify EPE into prognostic groups. METHODS AND RESULTS:We reviewed 1051 radical prostatectomy (RP) speci...


    pub_type: 杂志文章


    authors: Danneman D,Wiklund F,Wiklund NP,Egevad L

    更新日期:2013-10-01 00:00:00

  • Serum amyloid A immunohistochemical staining patterns in hepatitis.

    abstract:AIMS:Serum amyloid A is an acute phase reactant that is produced by hepatocytes in response to either inflammatory or neoplastic conditions. Because inflammatory adenomas produce this protein, serum amyloid A immunohistochemistry has been used in the evaluation of hepatocellular neoplasms. However, studies evaluating t...


    pub_type: 杂志文章


    authors: Piotti KC,Yantiss RK,Chen Z,Jessurun J

    更新日期:2016-12-01 00:00:00

  • Microscopical atrial amyloidosis in chronic heart disease.

    abstract::Nineteen out of 121 consecutive cardiac biopsies from 107 patients were found to contain amyloid deposits on routine Congo red screening. Seventeen were left atrial appendages removed during mitral valvotomy for chronic rheumatic mitral valve disease while the remaining two were right atrial appendages excised during ...


    pub_type: 杂志文章


    authors: Looi LM

    更新日期:1981-11-01 00:00:00

  • Warthin-like tumour of the thyroid gland: RET/PTC expression indicates it is a variant of papillary carcinoma.

    abstract:AIMS:Three cases with features of so-called 'Warthin-like tumour' of the thyroid (WaLTT) are described, in order to evaluate its relationship with papillary carcinoma (PC). METHODS AND RESULTS:We performed an histological and immunohistochemical study with emphasis on RET/PTC expression. The most striking features are...


    pub_type: 杂志文章


    authors: D'Antonio A,De Chiara A,Santoro M,Chiappetta G,Losito NS

    更新日期:2000-06-01 00:00:00

  • Differentiation of ovarian mucinous carcinoma and metastatic colorectal adenocarcinoma by immunostaining with beta-catenin.

    abstract:AIMS:To investigate whether localization of beta-catenin is helpful in differentiating primary ovarian mucinous carcinoma and colorectal adenocarcinoma metastatic to the ovary. Extra-ovarian cancers which metastasize to the ovaries, especially from colorectal adenocarcinoma, frequently mimic primary ovarian carcinomas,...


    pub_type: 杂志文章


    authors: Chou YY,Jeng YM,Kao HL,Chen T,Mao TL,Lin MC

    更新日期:2003-08-01 00:00:00

  • Evidence for differentiation in medulloblastomas appearing primitive on light microscopy: an ultrastructural study.

    abstract::Surgical biopsy specimens from five children with cerebellar medulloblastoma were examined for ultrastructural evidence of differentiating activity. At light microscope level, all the tumour samples appeared primitive and featureless, and could not be classified as showing either neuroblastic or spongioblastic differe...


    pub_type: 杂志文章


    authors: Moss TH

    更新日期:1983-11-01 00:00:00

  • The expression and prognostic significance of bcl-2-associated transcription factor 1 in rectal cancer following neoadjuvant therapy.

    abstract:AIMS:bcl-2-associated transcription factor 1 (BCLAF1) is a nuclear protein that binds to bcl-related proteins and can induce apoptosis and autophagy. The aim of this study was to investigate the expression of BCLAF1 in a series of rectal cancers following neoadjuvant therapy. METHODS AND RESULTS:Immunohistochemistry w...


    pub_type: 杂志文章


    authors: Brown GT,Cash B,Alnabulsi A,Samuel LM,Murray GI

    更新日期:2016-03-01 00:00:00

  • Atypical juvenile polyposis.

    abstract::Two cases of atypical juvenile polyposis are described in males of 9 months and 25 years-of-age. The first was associated with congenital megacolon and presented as juvenile polyps with features suggesting mild dysplasia. In the second case six histological lesions are found: I hyperplastic polyps; 2 juvenile polyps; ...


    pub_type: 杂志文章


    authors: Grigioni WF,Alampi G,Martinelli G,Piccaluga A

    更新日期:1981-07-01 00:00:00