Antibodies to cytokeratins bind to epitopes in human uterine smooth muscle cells in normal and pathological pregnancies.

Abstract:

:Cytokeratin antibodies have been widely used for the identification of trophoblast cells in the placental bed, following their invasion from the developing conceptus. Their identification centres upon the expression of cytokeratin in epithelial cells, from which trophoblast cells are derived. Our recent observations indicate that this strict relationship may be more complex than was thought. Cryostat and paraffin sections of human decidua and myometrium, taken from the placental bed and the uterotomy cut, were examined immunocytochemically for cytokeratins using ten antibody clones selected to identify different cytokeratin proteins and antigenic epitopes. Biopsy specimens were obtained from normal and pathological pregnancies (pre-eclampsia, fetal retardation, amnioninfection, hysterorrhexis, placenta praevia) at the time of caesarean section (26-41 weeks of pregnancy). Antibodies against nine clones, CAM 5.2, MNF 116, AE1/AE3, CK5, KS-B17.2, CY-90, M20, E3, and 34 beta E12 identified, as expected, syncytial giant cells and mononuclear trophoblasts within the placental bed and glandular epithelial cells throughout the uterus. In addition, they stained numerous fusiform cells that were classified by established criteria to represent smooth muscle cells, both within blood vessels and myometrium. No staining differences were observed between normal and pathological disorders. These results indicate that cytokeratin antibodies CAM 5.2, MNF 116 and AE1/AE3, and other antibodies targeting proteins 8 and 18, cross-react with epitopes expressed in cells other than giant trophoblastic cells and mononuclear trophoblasts in the uterus and, thus, caution has to be used when such antibodies are used for the diagnostic characterization of tissues related to the placental bed.

journal_name

Histopathology

journal_title

Histopathology

authors

Stiemer B,Graf R,Neudeck H,Hildebrandt R,Hopp H,Weitzel HK

doi

10.1111/j.1365-2559.1995.tb00303.x

subject

Has Abstract

pub_date

1995-11-01 00:00:00

pages

407-14

issue

5

eissn

0309-0167

issn

1365-2559

journal_volume

27

pub_type

杂志文章
  • Rhabdomyomatosis of the newborn lung unassociated with other malformations.

    abstract:AIMS:To describe a case of rhabdomyomatosis of the lung unassociated with other external or visceral malformations in a newborn infant. METHODS AND RESULTS:A 26 weeks gestation newborn male with no relevant medical or family history presented a well-circumscribed solid area in the posterior mediastinum occupying the u...

    journal_title:Histopathology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2559.1997.2830880.x

    authors: Hardisson D,García-Jiménez JA,Jiménez-Heffernan JA,Nistal M

    更新日期:1997-11-01 00:00:00

  • p53 expression in normal paraffin-embedded tissue using different antibodies and antigen retrieval buffer systems.

    abstract:AIMS:The study was undertaken to demonstrate wild-type p53 in normal paraffin-embedded tissues using two widely used antibodies, DO7 and 1801 and two different antigen retrieval buffer systems. METHODS AND RESULTS:Formalin-fixed paraffin-embedded normal tissue samples were obtained from the archives of the John Radcli...

    journal_title:Histopathology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2559.2003.01563.x

    authors: Pillai G,Roberts H,Gatter K,Pezzella F

    更新日期:2003-01-01 00:00:00

  • Liquid-based cytological and immunohistochemical study of nasopharyngeal swab from persons under investigation for SARS-CoV-2 infection.

    abstract:INTRODUCTION:We describe cytologic and immunohistologic findings in virus transport medium on cases under investigation of SARS-CoV-2 infection. METHODS:Cytologic findings in cases under investigation of SARS-CoV-2 infection from one hundred consecutive nasopharyngeal swab were reviewed. Immunohistochemistry and SARSC...

    journal_title:Histopathology

    pub_type: 杂志文章

    doi:10.1111/his.14257

    authors: Parada D,Peña KB,Gumà J,Guilarte C,Riu F

    更新日期:2020-09-24 00:00:00

  • Polymorphous low-grade adenocarcinoma of the salivary glands: a clinicopathological comparison with adenoid cystic carcinoma.

    abstract::Six cases of polymorphous low-grade adenocarcinoma (terminal duct adenocarcinoma) of the minor salivary glands are presented. In all but one there was a history of a painless intra-oral mass of fairly long duration. The histopathological appearances were characterized by cytological uniformity in a variety of morpholo...

    journal_title:Histopathology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2559.1991.tb00002.x

    authors: Simpson RH,Clarke TJ,Sarsfield PT,Gluckman PG,Babajews AV

    更新日期:1991-08-01 00:00:00

  • Patterns of SATB2 and p16 Reactivity Aid Distinction of Atypical Polypoid Adenomyoma from Myoinvasive Endometrioid Carcinoma and Benign Adenomyomatous Polyp on Endometrial Sampling.

    abstract:AIMS:Atypical polypoid adenomyoma (APAM) is an uncommon uterine lesion composed of complex endometrioid glands with frequent squamous morular metaplasia and fibromuscular stroma. On endometrial curettage, biopsy, or polypectomy specimens, the admixture of endometrioid glands and smooth muscle raises the differential di...

    journal_title:Histopathology

    pub_type: 杂志文章

    doi:10.1111/his.14338

    authors: Worrell HI,Sciallis AP,Skala SL

    更新日期:2021-01-18 00:00:00

  • Aggressive B-cell lymphomas with a primary bone marrow presentation.

    abstract::Aggressive B-cell lymphomas present as a heterogeneous spectrum of disease. A primary diagnosis in the bone marrow (BM) may be challenging in terms of diagnostic classification and clinical handling, owing to limited architectural information. Aggressive B-cell lymphomas can be subdivided into entities that typically ...

    journal_title:Histopathology

    pub_type: 杂志文章

    doi:10.1111/his.14124

    authors: Zamò A,Johnston P,Attygalle AD,Laurent C,Arber DA,Fend F

    更新日期:2020-09-01 00:00:00

  • Microvessel density compared with the Chalkley count in a prognostic study of angiogenesis in breast cancer patients.

    abstract:AIMS:Evaluation of angiogenesis by intratumoral vessel profiles can be performed by different methods. The aim of this study was to investigate the prognostic value of estimates obtained by the intratumoral microvessel density (MVD) method and then to compare with corresponding estimates obtained by the Chalkley method...

    journal_title:Histopathology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2559.2004.01848.x

    authors: Hansen S,Sørensen FB,Vach W,Grabau DA,Bak M,Rose C

    更新日期:2004-05-01 00:00:00

  • Variable fidelity of tissue-marking dyes in surgical pathology.

    abstract:AIMS:Pathology specimens often contain important margins that must be identified from gross examination of specimens through to microscopic examination. Commonly, unique colours of tissue-marking dye (TMD) are applied to each margin, which facilitates both macroscopic and microscopic identification. Various techniques ...

    journal_title:Histopathology

    pub_type: 杂志文章

    doi:10.1111/his.12328

    authors: Williams AS,Dakin Haché K

    更新日期:2014-05-01 00:00:00

  • Tumour regression grade (TRG) analyses in patients with resectable gastro-oesophageal adenocarcinomas treated with platinum-based neoadjuvant chemotherapy.

    abstract:AIMS:Neoadjuvant chemotherapy followed by surgery is the standard of care for patients with gastro-oesophageal adenocarcinoma. The aims were to validate the utility of the tumour regression grade (TRG) in patients who have received chemotherapy and to investigate if (i) TRG correlates with tumour downstaging and (ii) T...

    journal_title:Histopathology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2559.2009.03404.x

    authors: Fareed KR,Ilyas M,Kaye PV,Soomro IN,Lobo DN,Parsons SL,Madhusudan S

    更新日期:2009-10-01 00:00:00

  • Metastatic tumours in the testis.

    abstract::In a prospective autopsy study of male subjects with solic malignant neoplasms, six were shown to have metastatic deposits within the testis (2.5%). These were metastases from carcinoma of the prostate (two cases), melanoma (two cases), bronchial carcinoma (one case) and pleural mesothelioma (one case). In addition, f...

    journal_title:Histopathology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2559.1979.tb02979.x

    authors: Tiltman AJ

    更新日期:1979-01-01 00:00:00

  • The molecular pathogenesis of Hodgkin lymphoma.

    abstract::Hodgkin lymphoma (HL) is an unusual malignancy in that the tumour cells, the Hodgkin and Reed-Sternberg (HRS) cells, are a minor component of the tumour mass, the bulk of which is a mixed cellular infiltrate. There is compelling evidence that HRS cells are clonal B cells that have lost their B cell phenotype. Mature B...

    journal_title:Histopathology

    pub_type: 杂志文章,评审

    doi:10.1111/j.1365-2559.2010.03705.x

    authors: Farrell K,Jarrett RF

    更新日期:2011-01-01 00:00:00

  • Immunohistology of the human spleen: an inventory of the localization of lymphocyte subpopulations.

    abstract::The immunohistochemistry of the cell population of the human spleen was studied. As controls, a strictly defined group of five specimens were used. This study stresses the need for the utmost care in this selection procedure. Using enzyme- and immuno-histochemistry the lymphocyte subpopulations were studied as to thei...

    journal_title:Histopathology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2559.1986.tb02482.x

    authors: van Krieken JH,te Velde J

    更新日期:1986-03-01 00:00:00

  • Morphological patterns of the liver in South American blastomycosis.

    abstract::Histological features were semi-quantitatively analysed in 60 livers, obtained by necropsy, from patients who died of South American blastomycosis. These findings were classified and correlated with duration of the disease and treatment. Bile-duct lesions were prominent and a particular pattern was found in which segm...

    journal_title:Histopathology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2559.1978.tb01716.x

    authors: Teixeira F,Gayotto LC,De Brito T

    更新日期:1978-07-01 00:00:00

  • An ultrastructural study of a jejunal inflammatory fibroid polyp.

    abstract::Ultrastructural appearances of a small intestinal inflammatory fibroid polyp are reported. There is a vascular scaffolding, with interstices which contain regular and fibrous long-spacing type collagen, condensed lamellar basement membrane fibroblasts, basophils, some eosinophils and histiocytes. In addition other cel...

    journal_title:Histopathology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2559.1981.tb01777.x

    authors: Williams RM

    更新日期:1981-03-01 00:00:00

  • Promoter hypermethylation contributes to TIMP3 down-regulation in high stage endometrioid endometrial carcinomas.

    abstract:AIMS:Expression of tissue inhibitor of metalloproteinases-3 (TIMP-3) has been found to be decreased in several types of cancer by promoter gene hypermethylation. However, little is known regarding the silencing effect of TIMP3 promoter hypermethylation on gene and protein expression in endometrial carcinomas and its pr...

    journal_title:Histopathology

    pub_type: 杂志文章

    doi:10.1111/his.12047

    authors: Catasus L,Pons C,Muñoz J,Espinosa I,Prat J

    更新日期:2013-03-01 00:00:00

  • The helicase-like transcription factor is a strong predictor of recurrence in hypopharyngeal but not in laryngeal squamous cell carcinomas.

    abstract:AIMS:To examine the immunohistochemical expression of helicase-like transcription factor (HLTF) in relation to the prognosis of hypopharyngeal (HSCCs) and laryngeal (LSCCs) squamous cell carcinomas, and to characterize the HLTF protein variants expressed in biopsy specimens of head and neck squamous cell carcinoma (HNS...

    journal_title:Histopathology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2559.2009.03330.x

    authors: Capouillez A,Debauve G,Decaestecker C,Filleul O,Chevalier D,Mortuaire G,Coppée F,Leroy X,Belayew A,Saussez S

    更新日期:2009-07-01 00:00:00

  • Identifying HIV infection in diagnostic histopathology tissue samples--the role of HIV-1 p24 immunohistochemistry in identifying clinically unsuspected HIV infection: a 3-year analysis.

    abstract:AIMS:Because of the clinical difficulty in identifying the early stages of human immunodeficiency virus (HIV) infection, the histopathologist often has to consider the diagnosis of HIV in tissue samples from patients with no previous suspicion of HIV infection. The aim was to investigate the practicality and utility of...

    journal_title:Histopathology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2559.2010.03513.x

    authors: Moonim MT,Alarcon L,Freeman J,Mahadeva U,van der Walt JD,Lucas SB

    更新日期:2010-03-01 00:00:00

  • Partial regression in primary carcinoma of the lung: does it occur?

    abstract:AIMS:To study immunohistochemically a group of 28 primary lung cancers which demonstrated histological features reminiscent of those characteristic of regression in malignant melanoma, to determine the phenotype of their immune cell infiltrates. METHODS AND RESULTS:Using a standard s-ABC immunoperoxidase method then q...

    journal_title:Histopathology

    pub_type: 杂志文章

    doi:

    authors: Kerr KM,Johnson SK,King G,Kennedy MM,Weir J,Jeffrey R

    更新日期:1998-07-01 00:00:00

  • Apoptotic bodies and abnormally dividing epithelial cells in squamous cell carcinoma.

    abstract::Apoptotic bodies were investigated ultrastructurally in 10 cases of squamous cell carcinoma taken from buccal mucosa, tongue and larynx. Epithelial cells undergoing mitosis were found to show premature formation of nuclear membranes around various aggregates of chromosomal material giving the appearance of micronuclei...

    journal_title:Histopathology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2559.1986.tb02590.x

    authors: el-Labban NG,Osorio-Herrera E

    更新日期:1986-09-01 00:00:00

  • 'Pseudomesotheliomatous' carcinomas of the pleura: a 10-year analysis of cases from the Environmental Lung Disease Research Group, Cardiff.

    abstract:AIMS:To undertake a clinicopathological study of diffuse serosal neoplasms of epithelial histogenesis which clinically and pathologically mimic malignant pleural mesothelioma. METHODS AND RESULTS:Over a 10-year (1990-2000) study period 53 carcinomas mimicking diffuse pleural mesothelioma ('pseudomesotheliomatous' carc...

    journal_title:Histopathology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2559.2003.01674.x

    authors: Attanoos RL,Gibbs AR

    更新日期:2003-11-01 00:00:00

  • 'Atypical adenomatous hyperplasia' in liver cirrhosis: low-grade hepatocellular carcinoma or borderline lesion?

    abstract::Adenomatous hyperplasia, defined as a sizable parenchymal nodule in cirrhosis, was examined morphologically. Ninety-seven nodules of adenomatous hyperplasia were obtained from 47 cirrhotic livers and were divided into 'ordinary' (44 nodules) and 'atypical' (53 nodules) types. The former consisted of hepatocytes simila...

    journal_title:Histopathology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2559.1990.tb00660.x

    authors: Nakanuma Y,Terada T,Terasaki S,Ueda K,Nonomura A,Kawahara E,Matsui O

    更新日期:1990-07-01 00:00:00

  • Familial mediterranean fever and acute myocardial infarction secondary to coronary vasculitis.

    abstract:AIMS:We report a case study to elucidate the pathogenesis of polyarteritis nodosa (PAN) type vasculitis, a rare complication of familial mediterranean fever (FMF). METHODS AND RESULTS:A woman with amyloidosis complicating FMF underwent a cadaveric renal transplantation and 5 years later suffered an acute myocardial in...

    journal_title:Histopathology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2559.1998.00462.x

    authors: Serrano R,Martínez MA,Andrés A,Morales JM,Samartin R

    更新日期:1998-08-01 00:00:00

  • Tubular variant of basal cell adenoma shares immunophenotypical features with normal intercalated ducts and is closely related to intercalated duct lesions of salivary gland.

    abstract:AIMS:The morphological criteria for identification of intercalated duct lesions (IDLs) of salivary glands have been defined recently. It has been hypothesised that IDL could be a precursor of basal cell adenoma (BCA). BCAs show a variety of histological patterns, and the tubular variant is the one that presents the str...

    journal_title:Histopathology

    pub_type: 杂志文章

    doi:10.1111/his.12339

    authors: Montalli VA,Martinez E,Tincani A,Martins A,Abreu Mdo C,Neves C,Costa AF,Araújo VC,Altemani A

    更新日期:2014-05-01 00:00:00

  • Updates in the approach to intrathoracic sarcomas.

    abstract::Primary intrathoracic sarcomas constitute a rare, heterogeneous group of neoplasms that occur in the lung parenchyma, pleura, and mediastinum. Although pathologically diverse, they frequently show similar clinical and radiological features, and accurate histopathological diagnosis is therefore crucial for correct mana...

    journal_title:Histopathology

    pub_type: 杂志文章,评审

    doi:10.1111/his.12771

    authors: Thway K,Jordan S,Fisher C,Nicholson AG

    更新日期:2015-12-01 00:00:00

  • Nuclear expression of maspin is associated with a lower recurrence rate and a longer disease-free interval after surgery for squamous cell carcinoma of the larynx.

    abstract:AIMS:Maspin, a protein belonging to the serpin superfamily, is the product of a tumour suppressor gene. Tissue distribution studies have shown maspin expression in normal mammary epithelial cells, in the placenta, prostate, thymus, testis, oral cavity, small intestine, skin, and cornea. Maspin is expressed but down-reg...

    journal_title:Histopathology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2559.2005.02141.x

    authors: Marioni G,Blandamura S,Giacomelli L,Calgaro N,Segato P,Leo G,Fischetto D,Staffieri A,de Filippis C

    更新日期:2005-05-01 00:00:00

  • Dual-colour chromogenic in-situ hybridization is a potential alternative to fluorescence in-situ hybridization in HER2 testing.

    abstract:AIM:Dual-colour chromogenic in-situ hybridization (dc-CISH) is an emerging methodology for characterizing genomic alterations. This study was aimed at evaluating the performance of a dc-CISH kit (ZytoVision) in determining human epidermal growth factor receptor 2 (HER2) status in breast cancer. METHODS AND RESULTS:Two...

    journal_title:Histopathology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2559.2011.04037.x

    authors: Hwang CC,Pintye M,Chang LC,Chen HY,Yeh KY,Chein HP,Lee N,Chen JR

    更新日期:2011-11-01 00:00:00

  • A33 shows similar sensitivity to but is more specific than CDX2 as an immunomarker of colorectal carcinoma.

    abstract:AIMS:CDX2 is widely used as a sensitive and specific immunomarker for colorectal carcinoma (CRC), but neither its sensitivity nor its specificity is absolute. The aim of this study was to compare CDX1 and A33 with CDX2 as immunomarkers for CRC. METHODS AND RESULTS:As a pilot study, whole sections of 51 cases of liver ...

    journal_title:Histopathology

    pub_type: 杂志文章

    doi:10.1111/his.13194

    authors: Wong NACS,Adamczyk LA,Evans S,Cullen J,Oniscu A,Oien KA

    更新日期:2017-07-01 00:00:00

  • Immunohistochemical demonstration of p24 HTLV III major core protein in different cell types within lymph nodes from patients with lymphadenopathy syndrome (LAS).

    abstract::The p24 protein is the major core protein of LAV/HTLV III which is the putative agent of the lymphadenopathy syndrome. By the use of an anti-p24 monoclonal antibody we have studied the presence of LAV/HTLV III infected cells in 20 lymph nodes obtained from lymphadenopathy syndrome patients: 14 lymph nodes were charact...

    journal_title:Histopathology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2559.1986.tb02456.x

    authors: Baroni CD,Pezzella F,Mirolo M,Ruco LP,Rossi GB

    更新日期:1986-01-01 00:00:00

  • Unsuspected analgesic nephropathy in transitional cell carcinoma of the upper tract: a morphological study.

    abstract::Analgesic nephropathy is known to have a high incidence in Australia where, following the experience in Scandinavia, reports have been published for some time recording the association between analgesic nephropathy and urothelial malignancies. The morbid anatomical features of analgesic nephropathy are now sufficientl...

    journal_title:Histopathology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2559.1980.tb02920.x

    authors: Lomax-Smith J,Seymour AE

    更新日期:1980-05-01 00:00:00

  • A method to fix lipids for staining fat embolism in paraffin sections.

    abstract:AIMS:To develop a method to preserve lipids in formalin-fixed tissues for staining in paraffin sections, and to illustrate its use in lung and brain of a fat embolism case, and in examples of fatty liver and atheroma. METHODS AND RESULTS:A saturated solution of linoleic acid in 70% ethylene glycol was prepared and tis...

    journal_title:Histopathology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2559.2002.01414.x

    authors: Tracy RE,Walia P

    更新日期:2002-07-01 00:00:00