Abstract:
AIMS:CD30 immunohistochemistry (IHC) in malignant lymphoma is used for selection of patients in clinical trials using brentuximab vedotin, an antibody drug-conjugate targeting the CD30 molecule. For reliable implementation in daily practice and meaningful selection of patients for clinical trials, information on technical variation and interobserver reproducibility of CD30 immunohistochemistry (IHC) staining is required. METHODS AND RESULTS:We conducted a three-round reproducibility assessment of CD30 scoring for categorised frequency and intensity, including a technical validation, a 'live polling' pre- and post-instruction scoring round and a web-based round including individual scoring with additional IHC information to mimic daily diagnostic practice. Agreement in all three scoring rounds was poor to fair (κ = 0.12-0.35 for CD30-positive tumour cell percentage and κ = 0.16-0.41 for staining intensity), even when allowing for one category of freedom in percentage of tumour cell positivity (κ = 0.30-0.61). The first round with CD30 staining performed in five independent laboratories showed objective differences in staining intensity. In the second round, approximately half the pathologists changed their opinion on CD30 frequency after a discussion on potential pitfalls, highlighting hesitancy in decision-making. Using fictional cut-off points for percentage of tumour cell positivity, agreement was still suboptimal (κ = 0.35-0.60). CONCLUSIONS:Lack of agreement in cases with heterogeneous expression is shown to influence patient eligibility for treatment with brentuximab vedotin, both in clinical practice and within the context of clinical trials, and limits the potential predictive value of the relative frequency of CD30-positive neoplastic cells for clinical response.
journal_name
Histopathologyjournal_title
Histopathologyauthors
Koens L,van de Ven PM,Hijmering NJ,Kersten MJ,Diepstra A,Chamuleau M,de Jong Ddoi
10.1111/his.13647subject
Has Abstractpub_date
2018-09-01 00:00:00pages
473-482issue
3eissn
0309-0167issn
1365-2559journal_volume
73pub_type
杂志文章相关文献
HISTOPATHOLOGY文献大全abstract::Four cases of endocrine neoplasia within fibroadenomas of the breast are described. The tumours are identifiable by light microscopic, silver impregnation and ultrastructural studies. Argyrophilia and the presence of dense-core granules establish the identity of this tumour type. The differential diagnosis from, and p...
journal_title:Histopathology
pub_type: 杂志文章
doi:10.1111/j.1365-2559.1980.tb02936.x
更新日期:1980-07-01 00:00:00
abstract:AIMS:Diversion colitis (DC) is a localized, relatively benign, iatrogenic condition which occurs in almost 100% of diverted colonic segments in patients who undergo ileostomy/colostomy for various reasons. The aim of this study was to establish histological features of DC in children. METHODS AND RESULTS:Twenty-three ...
journal_title:Histopathology
pub_type: 杂志文章
doi:10.1046/j.1365-2559.2000.00803.x
更新日期:2000-01-01 00:00:00
abstract:AIMS:To assess the outcome of breast papillary lesions diagnosed by ultrasound-guided core needle biopsy (CB) or vacuum-assisted 'mammotome' biopsy (MT), the accuracy of these diagnoses, and whether it is justified not to undertake surgical excision of non-malignant papillary lesions so diagnosed. METHODS AND RESULTS:...
journal_title:Histopathology
pub_type: 杂志文章
doi:10.1111/his.12477
更新日期:2015-03-01 00:00:00
abstract::Epidermolysis bullosa, a rare genodermatosis, is characterized by increased skin fragility manifest as blistering and sometimes accompanied by scarring. The latter is particularly severe in the recessive dystrophic variant and may be complicated by the development of squamous carcinoma in up to 30% of patients. We hav...
journal_title:Histopathology
pub_type: 杂志文章
doi:10.1111/j.1365-2559.1992.tb00962.x
更新日期:1992-03-01 00:00:00
abstract::Recently, there has been intense interest in the study of gastrointestinal stromal tumour (GIST); one might call it a virtual GIST revolution. This is due largely to the realization that most GISTs express KIT and harbour activating c-KIT (KIT) or platelet-derived growth factor receptor-alpha (PDGFRA) receptor tyrosin...
journal_title:Histopathology
pub_type: 杂志文章,评审
doi:10.1111/j.1365-2559.2005.02291.x
更新日期:2006-01-01 00:00:00
abstract:AIMS:CDX2 is widely used as a sensitive and specific immunomarker for colorectal carcinoma (CRC), but neither its sensitivity nor its specificity is absolute. The aim of this study was to compare CDX1 and A33 with CDX2 as immunomarkers for CRC. METHODS AND RESULTS:As a pilot study, whole sections of 51 cases of liver ...
journal_title:Histopathology
pub_type: 杂志文章
doi:10.1111/his.13194
更新日期:2017-07-01 00:00:00
abstract:AIMS:To evaluate the independent prognostic value of apoptotic versus proliferative fractions in a series of 92 patients with non-Hodgkin's lymphomas (NHL). METHODS AND RESULTS:Apoptotic fractions were quantified by use of the TdT (terminal deoxynucleotidyl-transferase)-mediated in-situ end-labelling technique (TUNEL)...
journal_title:Histopathology
pub_type: 杂志文章
doi:10.1046/j.1365-2559.1998.00489.x
更新日期:1998-09-01 00:00:00
abstract:AIM:Achalasia is a disease of the oesophagus characterized by increased lower oesophageal sphincter (LOS) tone, absence of LOS relaxation with swallowing and aperistalsis of the body of the oesophagus. The aetiology and pathogenesis of idiopathic achalasia is still controversial. METHODS AND RESULTS:We examined 16 oes...
journal_title:Histopathology
pub_type: 杂志文章
doi:10.1046/j.1365-2559.1999.035005445.x
更新日期:1999-11-01 00:00:00
abstract::In the course of running two clinical trials between 1966 and 1985, data became available for 1315 patients, 713 in the first trial and 602 patients in the second trial, which has allowed comparison between histological findings in laryngeal and hypopharyngeal carcinoma, the observed survival and the tumour-free rates...
journal_title:Histopathology
pub_type: 杂志文章
doi:10.1111/j.1365-2559.1991.tb00230.x
更新日期:1991-11-01 00:00:00
abstract::Amyloidosis is a heterogeneous group of disorders characterized by extracellular deposition of abnormal protein fibrils which are derived from different proteins in different forms of the disease. Asymptomatic amyloid deposition in a variety of tissues is a universal accompaniment of ageing, and clinical amyloidosis i...
journal_title:Histopathology
pub_type: 杂志文章,评审
doi:10.1111/j.1365-2559.1994.tb00001.x
更新日期:1994-11-01 00:00:00
abstract:AIMS:Thymic carcinoma is rare and usually has a fatal outcome. Gene mutations in the epidermal growth factor receptor (EGFR) signalling pathway and TP53 have not been well analysed in thymic carcinoma. METHODS AND RESULTS:We examined a large cohort of thymic carcinoma and thymoma type A/B3 and looked for gene mutation...
journal_title:Histopathology
pub_type: 杂志文章
doi:10.1111/his.13936
更新日期:2019-11-01 00:00:00
abstract:AIMS:There is increasing evidence of Gleason score (GS) drift in prostatic core biopsies during the last two decades. The ProtecT study is a randomized controlled study and provides an excellent cohort to study the effect of time, prostate-specific antigen (PSA) level, perineural invasion, tumour length and age on GS. ...
journal_title:Histopathology
pub_type: 杂志文章,随机对照试验
doi:10.1111/his.12549
更新日期:2015-02-01 00:00:00
abstract:AIMS:We investigated the phenotypic and cytotoxic characteristics of different types of peripheral T-cell and NK-cell lymphomas and correlated the findings of cytotoxic phenotype with Epstein-Barr virus (EBV) association. METHODS AND RESULTS:Eighty cases of peripheral T-cell and NK-cell lymphomas, classified according...
journal_title:Histopathology
pub_type: 杂志文章
doi:10.1046/j.1365-2559.1999.00582.x
更新日期:1999-01-01 00:00:00
abstract::We reviewed 45 pulmonary B-cell non-Hodgkin's lymphomas to determine whether their morphology and immunohistochemical features were those of lymphomas arising from mucosa-associated lymphoid tissue (MALT), as described in other sites. The polymerase chain reaction was used to provide further information on clonality. ...
journal_title:Histopathology
pub_type: 杂志文章
doi:10.1111/j.1365-2559.1995.tb00246.x
更新日期:1995-05-01 00:00:00
abstract:AIMS:To establish the prevalence of CD44 protein expression in a large surgical series of Barrett's adenocarcinoma and associated preneoplastic lesions and to correlate this expression with clinicopathological data and prognosis. METHODS AND RESULTS:CD44H and variants (V4/V5, V6) expression was detected by immunohisto...
journal_title:Histopathology
pub_type: 杂志文章
doi:10.1046/j.1365-2559.1998.00316.x
更新日期:1998-01-01 00:00:00
abstract:AIMS:To investigate whether Glia maturation factor-beta (GMFB) is expressed in thymomas and is associated with T-cell development. METHODS AND RESULTS:We investigated the expression of GMFB by immunohistochemistry in 86 cases of thymoma classified into five type A, 35 type AB, 11 type B1, 26 type B2, and nine type B3 ...
journal_title:Histopathology
pub_type: 杂志文章
doi:10.1111/j.1365-2559.2005.02224.x
更新日期:2005-09-01 00:00:00
abstract::The benign 'Triton' tumour is a neural tumour with benign rhabdomyomatous differentiation: it must be distinguished from the better known malignant counterpart. We describe the first case outside the peripheral soft tissues and the first to be studied ultrastructurally. It also represents the second lesion identified ...
journal_title:Histopathology
pub_type: 杂志文章
doi:10.1111/j.1365-2559.1983.tb02268.x
更新日期:1983-07-01 00:00:00
abstract::This morphological study of small ducts with in situ breast carcinoma demonstrates the changes that occur in the adjacent stroma when it stains metachromatically with toluidine blue. They are indistinguishable from those reported in areas with infiltrative tumour growth, and are accompanied by progressive damage to th...
journal_title:Histopathology
pub_type: 杂志文章
doi:10.1111/j.1365-2559.1987.tb02610.x
更新日期:1987-01-01 00:00:00
abstract:AIM:We describe the unusual association of fallopian tubal prolapse and herpetic infection, an occurrence not previously reported to our knowledge. METHODS AND RESULTS:A 37-year-old woman presented with a small polypoid mass of the vaginal vault, 3 months after abdominal hysterectomy and abdominoplasty. The vaginal ma...
journal_title:Histopathology
pub_type: 杂志文章
doi:10.1111/j.1365-2559.1999.00644.x
更新日期:1999-06-01 00:00:00
abstract:AIMS:We have previously shown that hypertrophic scar tissue from burn patients contains abnormally high amounts of the proteoglycans versican and biglycan and reduced amounts of decorin, in comparison with normal dermis or mature scar. The lack of decorin may account for the poor organization of collagen fibrils in the...
journal_title:Histopathology
pub_type: 杂志文章
doi:10.1046/j.1365-2559.2000.00824.x
更新日期:2000-03-01 00:00:00
abstract:AIMS:Owing to their close embryological relationship, soft tissue perineurioma and meningioma may closely mimic each other, not only histologically but also by immunohistochemistry. We aimed to compare the frequency of perineurial marker expression in these two entities, and to determine whether somatostatin receptor 2...
journal_title:Histopathology
pub_type: 杂志文章
doi:10.1111/his.12366
更新日期:2014-07-01 00:00:00
abstract::One hundred and eighty-seven operable lung tumours were immunostained with the monoclonal antibody Ki-67 and divided into groups of high, moderate or low proliferation. Patients have been followed clinically for up to 7 years to ascertain whether this immunocytochemical measurement reflected tumour behaviour in terms ...
journal_title:Histopathology
pub_type: 杂志文章
doi:10.1111/j.1365-2559.1991.tb01503.x
更新日期:1991-12-01 00:00:00
abstract:AIMS:Glypican 3 (GPC3) has been reported to be overexpressed in yolk sac tumour (YST), but the sensitivity has not been compared with alpha-fetoprotein (AFP). YST can form numerous growth patterns and the expression of GPC3 in these patterns has not been studied. The aim was to address these aspects. METHODS AND RESUL...
journal_title:Histopathology
pub_type: 杂志文章
doi:10.1111/j.1365-2559.2010.03553.x
更新日期:2010-05-01 00:00:00
abstract::A review of 76 endomyocardial biopsies and 54 autopsies of patients with dilated cardiomyopathy (DCM) revealed features of hypertrophy and degenerative changes associated with long-standing hypertrophy. Long-term survivors had significantly heavier heart weights. Short-term survivors had heavier than normal right vent...
journal_title:Histopathology
pub_type: 杂志文章
doi:10.1111/j.1365-2559.1985.tb02821.x
更新日期:1985-04-01 00:00:00
abstract::Granular cell tumours rarely involve the lower respiratory tract. We report eight cases surgically resected at our institution. There were four females and four males, aged between 18 to 56 years (mean 40). One tumour associated with a peripheral lung adenocarcinoma was asymptomatic. The other lesions presented with o...
journal_title:Histopathology
pub_type: 杂志文章
doi:10.1111/j.1365-2559.1995.tb00218.x
更新日期:1995-09-01 00:00:00
abstract:AIMS:Pathological or neuroprotective mechanisms in the brain in severe malaria may arise from microvascular obstruction with malaria-parasitized erythrocytes. This study aimed to investigate the role of hypoxia and induction of the vascular endothelial growth factor (VEGF) pathway in the neuropathophysiology of severe ...
journal_title:Histopathology
pub_type: 杂志文章
doi:10.1111/j.1365-2559.2010.03619.x
更新日期:2010-08-01 00:00:00
abstract:AIMS:Eosinophilic oesophagitis (EoE) is a chronic inflammatory disease characterised clinically by symptoms of oesophageal dysfunction and histopathologically by a prominent eosinophilic inflammation. Despite eosinophils having a histologically predominant position, their role in the immunopathogenesis of the disease i...
journal_title:Histopathology
pub_type: 杂志文章
doi:10.1111/his.13653
更新日期:2018-09-01 00:00:00
abstract:AIM:To evaluate the intraobserver and interobserver reproducibility of the HER2 in-situ hybridization (ISH) test in breast cancer by measuring the impact of counting different numbers of invasive cancer cells. METHODS AND RESULTS:A cohort of 101 primary invasive breast cancer cases were evaluated for HER2 gene amplifi...
journal_title:Histopathology
pub_type: 杂志文章
doi:10.1111/his.13208
更新日期:2017-08-01 00:00:00
abstract::A male infant was diagnosed as having diabetes mellitus 1 d after birth and died on the third day. Histological examination of the pancreas showed small and inconspicuous islets of Langerhans that were completely devoid of insulin-secreting cells. There was no evidence of insulitis. ...
journal_title:Histopathology
pub_type: 杂志文章
doi:10.1111/j.1365-2559.1988.tb01974.x
更新日期:1988-05-01 00:00:00
abstract:AIMS:To determine the expression of matrix metalloproteinases (MMPs)-2, -7 and -9 and beta-catenin in uterine serous carcinoma (USC) and endometrioid endometrial carcinoma (EEC) and to investigate any difference in expression between EEC and USC which might explain the mechanism of invasion and aggressive behaviour of ...
journal_title:Histopathology
pub_type: 杂志文章
doi:10.1111/j.1365-2559.2007.02612.x
更新日期:2007-02-01 00:00:00