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The sperm stress test: a novel test that predicts pregnancy in assisted reproductive technologies.

Abstract：

OBJECTIVE:To develop a reliable sperm test that would predict pregnancy rate in assisted reproductive technologies. DESIGN:Blind prospective cohort study. SETTING:Tertiary-care, university hospital-affiliated IVF program. PATIENTS:One hundred nineteen sperm samples were obtained from 110 males from couples undergoing IVF or GIFT (ART). Sperm samples were washed by Percoll, incubated at 24 degrees C for 4 hours, and an aliquot of the same sperm suspension was used for ART incubated at 40 degrees C for 4 hours (stress test). Stress test scores are expressed as the ratio of final to initial motility. RESULTS:Of 119 ART cycles, 24 resulted in pregnancy. Of 24 pregnancies, 23 occurred in cycles that used sperm samples with stress test scores > or = 0.75 and only one with a stress test score < 0.75. The negative predictive value of the test, defined as the absence of pregnancy with scores < 0.75, was 98% and the positive predictive value, defined as the occurrence of pregnancy with scores > or = 0.75, was 36%. Logistic regression analysis indicated that the stress test score alone was correlated significantly with pregnancy after ART. CONCLUSION:These results indicate that stress test scores < 0.75 are predictive of poor pregnancy outcome in ART.

journal_name

Fertil Steril

journal_title

Fertility and sterility

authors

Alvarez JG,Minaretzis D,Barrett CB,Mortola JF,Thompson IE

doi

10.1016/s0015-0282(16)58107-2

subject

Has Abstract

pub_date

1996-02-01 00:00:00

pages

400-5

issue

2

eissn

0015-0282

issn

1556-5653

pii

S0015-0282(16)58107-2

journal_volume

65

pub_type

临床试验,杂志文章

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