Three-dimensional structure of a membrane-containing virus.

abstract：:Mast cells (MCs) are potent inflammatory cells that are distributed throughout mucosal barrier tissues and respond rapidly to pathogenic stimuli. During helminth infections, MCs play an important role as late-stage effectors. However, it is currently unknown whether MCs contribute to the early innate events that deter...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hepworth MR,Daniłowicz-Luebert E,Rausch S,Metz M,Klotz C,Maurer M,Hartmann S

更新日期：2012-04-24 00:00:00

abstract：:Cardiac myosin-binding protein C (cMyBP-C) is an accessory protein of striated muscle sarcomeres that is vital for maintaining regular heart function. Its 4 N-terminal regulatory domains, C0-C1-m-C2 (C0C2), influence actin and myosin interactions, the basic contractile proteins of muscle. Using neutron contrast variat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Whitten AE,Jeffries CM,Harris SP,Trewhella J

更新日期：2008-11-25 00:00:00

abstract：:Germ-line stem cells (GSCs) are maintained by the somatic microenvironment, or GSC niche, which ensures that GSCs can both self-renew and produce functional gametes. However, it remains unclear how the proper niche size and location are regulated within the developing gonads. In the Drosophila testis, the hub cells th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kitadate Y,Kobayashi S

更新日期：2010-08-10 00:00:00

abstract：:Genomes exhibit diverse patterns of species-specific GC content, GC and AT skews, codon bias, and mutation bias. Despite intensive investigations and the rapid accumulation of sequence data, the causes of these a priori different genome biases have not been agreed on and seem multifactorial and idiosyncratic. We show ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vetsigian K,Goldenfeld N

更新日期：2009-01-06 00:00:00

abstract：:Phosphorylation of inositol phospholipids plays a key role in cellular regulation via the generation of intracellular second messengers. In addition, it represents a mechanism to regulate interactions of the lipid bilayer with proteins and protein scaffolds involved in vesicle budding, cytoskeletal organization, and s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Guo J,Wenk MR,Pellegrini L,Onofri F,Benfenati F,De Camilli P

更新日期：2003-04-01 00:00:00

abstract：:Soluble epoxide hydrolase (sEH) is an emerging therapeutic target in a number of diseases that have inflammation as a common underlying cause. sEH limits tissue levels of cytochrome P450 (CYP) epoxides derived from omega-6 and omega-3 polyunsaturated fatty acids (PUFA) by converting these antiinflammatory mediators in...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： López-Vicario C,Alcaraz-Quiles J,García-Alonso V,Rius B,Hwang SH,Titos E,Lopategi A,Hammock BD,Arroyo V,Clària J

更新日期：2015-01-13 00:00:00

abstract：:We have inserted a fourth protein ligand into the zinc coordination polyhedron of carbonic anhydrase II (CAII) that increases metal affinity 200-fold (Kd = 20 fM). The three-dimensional structures of threonine-199-->aspartate (T199D) and threonine-199-->glutamate (T199E) CAIIs, determined by x-ray crystallographic met...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ippolito JA,Baird TT Jr,McGee SA,Christianson DW,Fierke CA

更新日期：1995-05-23 00:00:00

abstract：:The underlying causes of late-Holocene climate variability in the tropics are incompletely understood. Here we report a 1,500-year reconstruction of climate history and glaciation in the Venezuelan Andes using lake sediments. Four glacial advances occurred between anno Domini (A.D.) 1250 and 1810, coincident with sola...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Polissar PJ,Abbott MB,Wolfe AP,Bezada M,Rull V,Bradley RS

更新日期：2006-06-13 00:00:00

abstract：:Oxygen-derived free radicals have been implicated in the pathogenesis of vasogenic edema and infarction caused by ischemia and reperfusion injury. In earlier studies, exogenously supplied liposome-entrapped CuZn superoxide dismutase (CuZn-SOD) ameliorated ischemic brain edema and infarction in rats following focal cer...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kinouchi H,Epstein CJ,Mizui T,Carlson E,Chen SF,Chan PH

更新日期：1991-12-15 00:00:00

abstract：:The nuclear receptors LXRalpha and LXRbeta have been implicated in the control of cholesterol and fatty acid metabolism in multiple cell types. Activation of these receptors stimulates cholesterol efflux in macrophages, promotes bile acid synthesis in liver, and inhibits intestinal cholesterol absorption, actions that...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Joseph SB,McKilligin E,Pei L,Watson MA,Collins AR,Laffitte BA,Chen M,Noh G,Goodman J,Hagger GN,Tran J,Tippin TK,Wang X,Lusis AJ,Hsueh WA,Law RE,Collins JL,Willson TM,Tontonoz P

更新日期：2002-05-28 00:00:00

abstract：:A series of small peptides corresponding to the amino termini of the fibrin alpha- and beta-chains has been synthesized. The peptides glycyl-L-prolyl-L-arginyl-L-proline and glycyl-L-prolyl-L-arginylsarcosine are potent inhibitors of fibrin polymerization. Moreover, these peptides have a natural stability stemming fro...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Laudano AP,Doolittle RF

更新日期：1978-07-01 00:00:00

abstract：:Here, we identified and characterized a Ly49 family member, designated as Ly49Q. The Ly49q gene encodes a 273-aa protein with an immunoreceptor tyrosine-based inhibitory motif (ITIM) at the N terminus of its cytoplasmic domain. We show that the ITIM of Ly49Q can recruit SHP-2 and SHP-1 in a tyrosine phosphorylation-de...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Toyama-Sorimachi N,Tsujimura Y,Maruya M,Onoda A,Kubota T,Koyasu S,Inaba K,Karasuyama H

更新日期：2004-01-27 00:00:00

abstract：:The receptor for acetylcholine was partially purified by affinity chromatography of an extract in Triton X-100 of membrane fragments from electric tissue. The receptor was assayed, after its reduction with dithiothreitol, by reaction with the affinity-alkylating agent, [methyl-(3)H]4-(N-maleimido)-benzyltrimethylammon...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Karlin A,Cowburn D

更新日期：1973-12-01 00:00:00

abstract：:We have investigated whether glycosylation of membrane glycoproteins is a determinant of the site of maturation of enveloped viruses in Madin-Darby canine kidney (MDCK) cells. In MDCK cell monolayers, vesicular stomatitis virus buds exclusively from the basal or lateral plasma membranes and contains a sialylated glyco...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Roth MG,Fitzpatrick JP,Compans RW

更新日期：1979-12-01 00:00:00

abstract：:The brain contains an astonishing diversity of neurons, each expressing only one set of ion channels out of the billions of potential channel combinations. Simple organizing principles are required for us to make sense of this abundance of possibilities and wealth of related data. We suggest that energy minimization s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hasenstaub A,Otte S,Callaway E,Sejnowski TJ

更新日期：2010-07-06 00:00:00

abstract：:An important goal in biology is to predict from sequence data the high-resolution structures of proteins and the interactions that occur between them. In this paper, we describe a computational approach that can make these types of predictions for a series of coiled-coil dimers. Our method comprises a dual strategy th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Keating AE,Malashkevich VN,Tidor B,Kim PS

更新日期：2001-12-18 00:00:00

abstract：:A promoterless trp-lac fusion fragment was inserted near one end of the bacterial transposon Tn5 in the correct orientation to fuse lacZ gene expression to promoters outside Tn5. The resulting transposon, Tn5 lac, retains the kanamycin-resistance gene of Tn5 and transposes in Escherichia coli at 6% the frequency of Tn...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kroos L,Kaiser D

更新日期：1984-09-01 00:00:00

abstract：:The protein-protein interaction (PPI) network is crucial for cellular information processing and decision-making. With suitable inputs, PPI networks drive the cells to diverse functional outcomes such as cell proliferation or cell death. Here, we characterize the structural controllability of a large directed human PP...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vinayagam A,Gibson TE,Lee HJ,Yilmazel B,Roesel C,Hu Y,Kwon Y,Sharma A,Liu YY,Perrimon N,Barabási AL

更新日期：2016-05-03 00:00:00

abstract：:Trisomy is a genetic abnormality of considerable medical importance. The most familiar example is trisomy 21, which causes Down Syndrome [Cummings, M. R. (1988) Human Heredity: Principles and Issues (West Publishing Company, New York)]. In a classic paper, Axelrod and Hamilton [Axelrod, R. & Hamilton, W. D. (1981) Sci...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Day T,Taylor PD

更新日期：1998-03-03 00:00:00

abstract：:The spatial coordination of growth is of central importance for the regulation of plant tissue architecture. Individual layers, such as the epidermis, are clonally propagated and structurally maintained by symmetric cell divisions that are oriented along the plane of the layer. The developmental control of this proces...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Enugutti B,Kirchhelle C,Oelschner M,Torres Ruiz RA,Schliebner I,Leister D,Schneitz K

更新日期：2012-09-11 00:00:00

abstract：:Human IL-10 (hIL-10) modulates critical immune and inflammatory responses by way of interactions with its high- (IL-10R1) and low-affinity (IL-10R2) cell surface receptors. Human cytomegalovirus exploits the IL-10 signaling pathway by expressing a functional viral IL-10 homolog (cmvIL-10), which shares only 27% sequen...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jones BC,Logsdon NJ,Josephson K,Cook J,Barry PA,Walter MR

更新日期：2002-07-09 00:00:00

abstract：:Membrane-mediated particle interactions depend both on the properties of the particles themselves and the membrane environment in which they are suspended. Experiments have shown that chiral rod-like inclusions dissolved in a colloidal membrane of opposite handedness assemble into colloidal rafts, which are finite-siz...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Miller JM,Joshi C,Sharma P,Baskaran A,Baskaran A,Grason GM,Hagan MF,Dogic Z

更新日期：2019-08-06 00:00:00

abstract：:A method for assessing genomic similarity based on relative abundances of short oligonucleotides in large DNA samples is introduced. The method requires neither homologous sequences nor prior sequence alignments. The analysis centers on (i) dinucleotide (and tri- and tetra-) relative abundance extremes in genomic sequ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Karlin S,Ladunga I

更新日期：1994-12-20 00:00:00

abstract：:The endodermis acts as a "second skin" in plant roots by providing the cellular control necessary for the selective entry of water and solutes into the vascular system. To enable such control, Casparian strips span the cell wall of adjacent endodermal cells to form a tight junction that blocks extracellular diffusion ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hosmani PS,Kamiya T,Danku J,Naseer S,Geldner N,Guerinot ML,Salt DE

更新日期：2013-08-27 00:00:00

abstract：:Retinoblastoma-related (RBR) proteins regulate cell division in higher eukaryotes by controlling the adenovirus E2 promoter binding factor (E2F)/dimerization partner (DP) family of transcription factors that regulate expression of many genes involved in cell-cycle progression. We identified a previously undescribed me...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sabelli PA,Dante RA,Leiva-Neto JT,Jung R,Gordon-Kamm WJ,Larkins BA

更新日期：2005-09-13 00:00:00

abstract：:Transcriptional network evolution is central to the development of complex biological systems. Networks can evolve through variation of master regulators and/or by changes in regulation of genes within networks. To gain insight into meaningful evolutionary differences in large networks, it is essential to address the ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jegga AG,Inga A,Menendez D,Aronow BJ,Resnick MA

更新日期：2008-01-22 00:00:00

abstract：:Profiling the dynamic interaction of p300 with proximal promoters of human T cells identified a class of genes that rapidly coassemble p300 and RNA polymerase II (pol II) following mitogen stimulation. Several of these p300 targets are immediate early genes, including FOS, implicating a prominent role for p300 in the ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Byun JS,Wong MM,Cui W,Idelman G,Li Q,De Siervi A,Bilke S,Haggerty CM,Player A,Wang YH,Thirman MJ,Kaberlein JJ,Petrovas C,Koup RA,Longo D,Ozato K,Gardner K

更新日期：2009-11-17 00:00:00

abstract：:It is well established that exposure to solar UVB (290-320 nm) gives rise to mutations in oncogenes and tumor suppressor genes that initiate the molecular cascade toward skin cancer. Although UVA (320-400 nm) has also been implicated in multistage photocarcinogenesis, its potential contribution to sunlight mutagenesis...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Drobetsky EA,Turcotte J,Châteauneuf A

更新日期：1995-03-14 00:00:00

abstract：:The central structural feature of natural proteins is a tightly packed and highly ordered hydrophobic core. If some measure of exquisite, native-like core packing is necessary for enzymatic function, this would constitute a significant obstacle to the development of novel enzymes, either by design or by natural or exp...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Axe DD,Foster NW,Fersht AR

更新日期：1996-05-28 00:00:00

abstract：:The counterintuitive features of quantum physics challenge many common-sense assumptions. In an interferometric quantum eraser experiment, one can actively choose whether or not to erase which-path information (a particle feature) of one quantum system and thus observe its wave feature via interference or not by perfo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ma XS,Kofler J,Qarry A,Tetik N,Scheidl T,Ursin R,Ramelow S,Herbst T,Ratschbacher L,Fedrizzi A,Jennewein T,Zeilinger A

更新日期：2013-01-22 00:00:00