Abstract:
:In this study, we assessed the underlying mechanisms by which proteinases released from activated neutrophils mediate fibronectin degradation. Purified human neutrophils (1 x 10(6)) were incubated for 1 hr with 1 microM PMA in the absence or presence of different proteinase inhibitors in 96-well microtiter plates that were coated with 125I-labeled fibronectin (FN). PMA-activated neutrophils caused 85% of FN to be degraded (versus 5% under control conditions). A selective inhibitor of elastase (L658,758), a monoclonal antibody directed against human neutrophilic elastase, and plasma all reduced the neutrophil-mediated FN degradation by 60%. A monoclonal antibody directed against the neutrophil adhesion glycoprotein CD11/CD18 increased the antiproteolytic effect of plasma to 70% but had no effect on the other anti-elastase agents, suggesting that the subjacent space formed by adherent neutrophils restricted to a small degree plasma derived antiproteinases. Agents that blocked cathepsin G or cathepsin G and elastase completely prevented the proteolysis associated with PMA-stimulated neutrophils, suggesting that the actions of elastase may be dependent on the presence of biologically active cathepsin G.
journal_name
Inflammationjournal_title
Inflammationauthors
Kubes P,Smith R,Grisham MD,Granger DNdoi
10.1007/BF00918993subject
Has Abstractpub_date
1993-06-01 00:00:00pages
321-32issue
3eissn
0360-3997issn
1573-2576journal_volume
17pub_type
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