当前位置： SCI文献检索 > MOLECULAR AND CELLULAR ENDOCRINOLOGY期刊下所有文献 > Lactogenic hormones and extracellular matrix regulate expression of IGF-1 linked to MMTV-LTR in mammary epithelial cells.

Lactogenic hormones and extracellular matrix regulate expression of IGF-1 linked to MMTV-LTR in mammary epithelial cells.

Abstract：

:The cell line MD-IGF-1, containing an ovine IGF-1 cDNA driven by the mouse mammary tumor virus-long terminal repeat (MMTV-LTR) promoter, was used to study expression of IGF-1 linked to the MMTV-LTR in bovine mammary epithelial cells in response to various hormonal and substratum stimuli. Acute sensitivity of the MMTV-LTR promoter to glucocorticoids and sex steroids was ascertained by transient transfection of parental MAC-T cells with an MMTV-CAT construct. Specifically, CAT activity was induced by glucocorticoids, but not by 17 beta-estradiol or progesterone. Induction of MD-IGF-1 cells with dexamethasone (DEX) alone triggered a 29.5-fold increase in secretion of recombinant IGF-1 (348.9 vs 11.8 pg/micrograms DNA), and stimulated a 1.7-fold increase in total DNA within 72 h. Growth of MD-IGF-1 cells was enhanced by exogenous IGF-1, insulin, and TGF-alpha. In contrast, TGF-beta inhibited cell proliferation, while epidermal growth factor, estrogen, progesterone, and testosterone had no effect. Extracellular matrix from the Engelbreth-Holm-Swarm (EHS) tumor, in the presence of DEX, prolactin (PRL), and insulin stimulated a 29.4-fold increase in secretion of IGF-1 (591.9 pg/microgram DNA), compared with cells in absence of hormones (20.1 pg/micrograms DNA). EHS and DEX plus PRL triggered a 63.2-fold increase in IGF-1 secretion (689.1 pg/micrograms DNA), compared with MD-IGF-1 cells cultured on plastic (10.9 pg/micrograms DNA), in the absence of hormones. These data indicate that the MMTV-LTR is regulated by both lactogenic hormones and extracellular matrix in MD-IGF-1 cells and that the MMTV-LTR may be a useful regulatory element for targeting expression of foreign proteins in bovine mammary epithelial cells.

journal_name

Mol Cell Endocrinol

journal_title

Molecular and cellular endocrinology

authors

Romagnolo D,Akers RM,Wong EA,Boyle PL,McFadden TB,Byatt JC,Turner JD

doi

10.1016/0303-7207(93)90105-s

subject

Has Abstract

pub_date

1993-10-01 00:00:00

pages

147-57

issue

1-2

eissn

0303-7207

issn

1872-8057

journal_volume

96

pub_type

杂志文章

相关文献

MOLECULAR AND CELLULAR ENDOCRINOLOGY文献大全
  • Testosterone increases CCL-2 expression in visceral adipose tissue from obese women of reproductive age.

    abstract：CONTEXT:Hyperandrogenic states and obesity in women are associated with insulin-resistance. Androgens reduce glucose uptake in adipose cells and increase TNFα production in peripheral monocytes. Inflammatory cytokines have a known detrimental effect on insulin resistance. The aim of the present study was to explore the...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2017.01.051

    authors： Crisosto N,Flores C,Maliqueo M,Echiburú B,Vásquez J,Maluenda F,Sir-Petermann T

    更新日期：2017-03-15 00:00:00

  • Functional interactions of an upstream enhancer of the mouse glycoprotein hormone alpha-subunit gene with proximal promoter sequences.

    abstract：:Transcription of the glycoprotein hormone alpha-subunit gene in the pituitary is governed by different promoter elements in thyrotropes and gonadotropes. We recently identified an upstream enhancer that directs a high level of cell type specific expression in transgenic mice and stimulates proximal promoter activity i...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/s0303-7207(98)00110-5

    authors： Wood WM,Dowding JM,Sarapura VD,McDermott MT,Gordon DF,Ridgway EC

    更新日期：1998-07-25 00:00:00

  • Identification and androgen-regulated expression of two major human glandular kallikrein-1 (hGK-1) mRNA species.

    abstract：:The screening of an oligo(dT)-primed prostate cDNA library with a human glandular kallikrein-1 (hGK-1) genomic DNA fragment resulted in the isolation of two different hGK-1 cDNAs. A 1.2 kb cDNA (pGK-1) contains an open reading frame of 510 bp, encoding the major part of the previously predicted hGK-1 protein (Schedlic...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(91)90272-t

    authors： Riegman PH,Vlietstra RJ,van der Korput HA,Romijn JC,Trapman J

    更新日期：1991-04-01 00:00:00

  • Enhanced activity of the cardiac endocrine system during right ventricular hypertrophy.

    abstract：:In a model of pulmonary hypertension induced by a single injection of monocrotaline (MCT), we observed a time-dependent right ventricular hypertrophy, which became apparent in treated rats 21 days after administration of MCT and progressed through day 45. Associated with this right ventricular hypertrophy were time-de...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(89)90011-7

    authors： Oehlenschlager WF,Kurtz DT,Baron DA,Currie MG

    更新日期：1989-04-01 00:00:00

  • Phenylphenols, biphenols, bisphenol-A and 4-tert-octylphenol exhibit alpha and beta estrogen activities and antiandrogen activity in reporter cell lines.

    abstract：:We previously demonstrated the interactions of different chemical compounds with estrogen receptors ERalpha and ERbeta and the androgen receptor (AR) using different reporter cell lines. In this study, we characterize the ERalpha, ERbeta and AR activity of different biphenyls using the same tools. We provide evidence ...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/s0303-7207(02)00094-1

    authors： Paris F,Balaguer P,Térouanne B,Servant N,Lacoste C,Cravedi JP,Nicolas JC,Sultan C

    更新日期：2002-07-31 00:00:00

  • Compartmentalization of luteinizing hormone pools: dynamics of gonadotropin releasing hormone action in superfused pituitary cells.

    abstract：:Cultured rat anterior pituitary cells were continuously perfused with medium 199, and exposed to short (5 min), intermediate (30 min), or long (6 h) pulses of a maximally effective concentration of gonadotropin-releasing hormone (GnRH). Assay of the effluent by radioimmunoassay and interstitial-cell bioassay revealed ...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(82)90110-1

    authors： Naor Z,Katikineni M,Loumaye E,Vela AG,Dufau ML,Catt KJ

    更新日期：1982-07-01 00:00:00

  • Cell cycle regulation during early mouse embryogenesis.

    abstract：:Elaboration of a multicellular organism requires highly efficient coordination between proliferation and developmental processes. Accordingly, the embryonic cell cycle exhibits a high degree of plasticity; however, the mechanisms underlying its regulation in vivo remain largely unknown. The purpose of this review is t...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章,评审

    doi：10.1016/j.mce.2007.11.008

    authors： Artus J,Cohen-Tannoudji M

    更新日期：2008-01-30 00:00:00

  • Differential thyroid hormone sensitivity of fast cycling progenitors in the neurogenic niches of tadpoles and juvenile frogs.

    abstract：:Adult neurogenesis occurs in neural stem cell (NSC) niches where slow cycling stem cells give rise to faster cycling progenitors. In the adult mouse NSC niche thyroid hormone, T3, and its receptor TRα act as a neurogenic switch promoting progenitor cell cycle completion and neuronal differentiation. Little is known ab...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2015.11.026

    authors： Préau L,Le Blay K,Saint Paul E,Morvan-Dubois G,Demeneix BA

    更新日期：2016-01-15 00:00:00

  • Hypoxia decreases Runx2/Cbfa1 expression in human osteoblast-like cells.

    abstract：:To elucidate the molecular mechanism in relation to vascular supply and osteoporosis, we investigated the effect of hypoxia on Runx2 expression in MG63 cells. Also investigated was expression of type I collagen and osteocalcin, which are regulated by Runx2, alkaline phosphatase (ALPase) to see if they are affected by ...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/s0303-7207(02)00036-9

    authors： Park JH,Park BH,Kim HK,Park TS,Baek HS

    更新日期：2002-06-28 00:00:00

  • Functional analysis of LHbeta knockout mice.

    abstract：:LH and FSH act on gonadal cells to regulate steroidogenesis and gametogenesis. To model human reproductive disorders involving loss of LH function and to define LH-responsive genes, we produced knockout mice lacking the hormone-specific LHbeta subunit. LHbeta null mice are viable but demonstrate postnatal defects in g...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章,评审

    doi：10.1016/j.mce.2006.10.020

    authors： Kumar TR

    更新日期：2007-04-15 00:00:00

  • A genome-wide assessment of adrenocorticotropin action in the Y1 mouse adrenal tumor cell line.

    abstract：:This report summarizes the genome-wide effects of ACTH on transcript accumulation in mouse adrenal Y1 cells and the relative contributions of the cAMP-, protein kinase C- and Ca(2+)-dependent signaling pathways to these actions of the hormone. ACTH affected the accumulation of 1386 transcripts, a much larger number th...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章,评审

    doi：10.1016/j.mce.2006.12.024

    authors： Schimmer BP,Cordova M,Cheng H,Tsao A,Morris Q

    更新日期：2007-02-01 00:00:00

  • Induction of Gnrh mRNA expression by the ω-3 polyunsaturated fatty acid docosahexaenoic acid and the saturated fatty acid palmitate in a GnRH-synthesizing neuronal cell model, mHypoA-GnRH/GFP.

    abstract：:Gonadotropin-releasing hormone (GnRH) neurons coordinate reproduction. However, whether GnRH neurons directly sense free fatty acids (FFAs) is unknown. We investigated the individual effects of the FFAs docosahexaenoic acid (DHA), palmitate, palmitoleate, and oleate (100 μM each) on Gnrh mRNA expression in the mHypoA-...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2016.02.019

    authors： Tran DQ,Ramos EH,Belsham DD

    更新日期：2016-05-05 00:00:00

  • Inhibitory effect of gonadotropin releasing hormone upon cultured testicular cells.

    abstract：:The direct inhibitory of gonadotropin-releasing hormone (GnRH) upon testicular androgen production was studied in cultured testicular cells. Enzyme-dispersed testicular cells from immature hypophysectomized rats were cultured for 6 days in a serum-free medium with or without various hormones. Culture media were change...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(81)90029-0

    authors： Hsueh AJ,Schreiber JR,Erickson GF

    更新日期：1981-01-01 00:00:00

  • A comparative study of the role of adenylate cyclase in the release of adrenocorticotropin from the ovine and rat anterior pituitary.

    abstract：:The interaction between corticotropin-releasing factor (CRF) and arginine vasopressin (AVP) is important in the regulation of adrenocorticotropin (ACTH) release from the anterior pituitary (AP). CRF exerts its effect on the AP by activating the adenylate cyclase (AC) complex whereas AVP increases the turnover of phosp...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(94)90232-1

    authors： Liu JP,Robinson PJ,Funder JW,Engler D

    更新日期：1994-05-01 00:00:00

  • The anti-diabetic AMPK activator AICAR reduces IL-6 and IL-8 in human adipose tissue and skeletal muscle cells.

    abstract：:Increased production of inflammatory cytokines is suggested to be of importance for initiation and progression of insulin resistance. Chronic treatment with the AMP analogue 5-aminoimidazole-4-carboxamide-1-beta-d-ribonucleoside (AICAR) has been shown to improve insulin sensitivity and prevent the development of diabe...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2008.06.004

    authors： Lihn AS,Pedersen SB,Lund S,Richelsen B

    更新日期：2008-09-24 00:00:00

  • Cannabinoid receptor I activation markedly inhibits human decidualization.

    abstract：:The role of cannabinoid receptor I (CBR-1) in the induction of decidualization was examined using decidual fibroblasts and human endometrial stromal cells as model systems. Decidual fibroblasts decidualized in vitro for 3 and 6 days in the presence of the CBR-1 agonist R(+)-WIN 55,212-2 mesylate (WIN, 0.1-10 microM) e...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2004.09.007

    authors： Moghadam KK,Kessler CA,Schroeder JK,Buckley AR,Brar AK,Handwerger S

    更新日期：2005-01-14 00:00:00

  • Characterization and regulation of a mRNA encoding the prostaglandin F2alpha receptor in the rat ovary.

    abstract：:The recent cloning of several cDNAs encoding prostaglandin (PG) receptors has paved the way for a more detailed investigation of the postulated regulatory role of prostaglandins in corpus luteum function. We have utilized the reverse transcription-polymerase chain reaction (RT-PCR) to isolate a mRNA encoding the ovari...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(96)03894-4

    authors： Olofsson JI,Leung CH,Bjurulf E,Ohno T,Selstam G,Peng C,Leung PC

    更新日期：1996-10-14 00:00:00

  • Chronic restraint stress induces serotonin transporter expression in the rat adrenal glands.

    abstract：:Chronic restraint stress (CRS) magnifies restraint-induced corticosterone secretion through a mechanism involving increased adrenocortical 5-HT content and turnover. We analysed the impact of CRS on serotonin transporter (SERT) expression and distribution in rat adrenal glands. Male Wistar rats were submitted to CRS (...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2020.110935

    authors： Shanker S,Saroj N,Cordova EJ,Jarillo-Luna RA,López-Sánchez P,Terrón JA

    更新日期：2020-12-01 00:00:00

  • Action of hydrocortisone at a translational level in the rat liver.

    abstract：:Administration of hydrocortisone to adrenalectomised rats elevated levels of both free and membrane-bound polysomes in liver and led to marked shifts in their sedimentation profiles in favour of heavier polysomes. The rate of nascent polypeptide formation was also found to be enhanced in response to the hormone but th...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(76)90038-1

    authors： Kulkarni SB,Netrawali MS,Pradhan DS,Sreenivasan A

    更新日期：1976-02-01 00:00:00

  • Sex- and age-associated differences in episodic-like memory and transcriptional regulation of hippocampal steroidogenic enzymes in rats.

    abstract：:The aim of this study was to evaluate the episodic-like memory (ELM) and the transcriptional regulation of the enzymes involved in hippocampal allopregnanolone synthesis in young adult and middle-aged male and female rats. Young adult males, but not middle-aged ones, showed a good performance in the ELM task. In contr...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2017.11.001

    authors： Rossetti MF,Varayoud J,Andreoli MF,Stoker C,Luque EH,Ramos JG

    更新日期：2018-07-15 00:00:00

  • The role of the renin-angiotensin system in the development of insulin resistance in skeletal muscle.

    abstract：:The canonical renin-angiotensin system (RAS) involves the initial action of renin to cleave angiotensinogen to angiotensin I (ANG I), which is then converted to ANG II by the angiotensin converting enzyme (ACE). ANG II plays a critical role in numerous physiological functions, and RAS overactivity underlies many condi...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章,评审

    doi：10.1016/j.mce.2012.04.011

    authors： Henriksen EJ,Prasannarong M

    更新日期：2013-09-25 00:00:00

  • A diabetes insipidus vasopressin prohormone altered outside the central core of neurophysin accumulates in the endoplasmic reticulum.

    abstract：:Over 20 mutations affecting the neurophysin moiety of the vasopressin prohormone, have been identified in families suffering from familial neurohypophysial diabetes insipidus (FNDI). Only one of these, NP87E-->stop, is located outside the central conserved domain implicated in sorting of the vasopressin prohormone. To...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/s0303-7207(00)00288-4

    authors： Nijenhuis M,Zalm R,Burbach JP

    更新日期：2000-09-25 00:00:00

  • Yin Yang 1 is a multi-functional regulator of adipocyte differentiation in 3T3-L1 cells.

    abstract：:Yin Yang 1 (YY1) is an ubiquitously distributed transcription factor that belongs to the GLI-Kruppel class of zinc finger proteins. The mechanism by which YY1 regulates adipocyte differentiation remains unclear. In this study, we investigated the functional role of YY1 during adipocyte differentiation. During the earl...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2015.06.035

    authors： Han Y,Choi YH,Lee SH,Jin YH,Cheong H,Lee KY

    更新日期：2015-09-15 00:00:00

  • Effect of the TYK-2/STAT-3 pathway on lipid accumulation induced by mono-2-ethylhexyl phthalate.

    abstract：BACKGROUND:Mono-2-ethylhexyl phthalate (MEHP), an important metabolite of di (2-ethylhexyl) phthalate (DEHP), can induce lipid metabolic disorder. Previous studies have shown that MEHP promotes 3T3-L1 cell differentiation; however, the underlying mechanism is unclear. The present study was performed to investigate the ...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2019.01.012

    authors： Qi W,Zhou L,Zhao T,Ding S,Xu Q,Han X,Zhao Y,Song X,Zhao T,Zhang X,Ye L

    更新日期：2019-03-15 00:00:00

  • Association of Ad4BP/SF-1 transcription factor with steroidogenic activity in oncogene-transformed granulosa cells.

    abstract：:Adrenal binding protein 4 (Ad4BP) known also as steroidogenic factor 1 (SF-1) is a cell specific transcription factor regulating all steroidogenic P450 genes and is present exclusively in steroidogenic tissues. In this study, we examined whether Ad4BP expression is affected by oncogene-induced cell transformation. Usi...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/s0303-7207(96)03989-5

    authors： Keren-Tal I,Dantes A,Plehn-Dujowich D,Amsterdam A

    更新日期：1997-03-14 00:00:00

  • Primate FSH-receptor promoter nucleotide sequence heterogeneity affects FSH-receptor transcription.

    abstract：:Follicle-stimulating hormone (FSH) is essential for primate reproduction and acts via the FSH-receptor (FSHR). Although the FSHR expression is highly cell-specific, knowledge of the FSHR promoter and its transcriptional regulation is very limited. We applied a comparative genomic approach of important primate lineages...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2009.12.020

    authors： Brune M,Adams C,Gromoll J

    更新日期：2010-04-12 00:00:00

  • The spatial and temporal regulation of the hormonal signal. Role of mitochondria in the formation of a protein complex required for the activation of cholesterol transport and steroids synthesis.

    abstract：:The mitochondria are critical for steroidogenesis since the ability of cholesterol to move into mitochondria to be available for cytochrome P450, CYP11A1, determines the efficacy of steroid production. Several proteins kinases, such as PKA, MEK and ERK which are essential to complete steroidogenesis, form a mitochondr...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章,评审

    doi：10.1016/j.mce.2012.12.024

    authors： Poderoso C,Duarte A,Cooke M,Orlando U,Gottifredi V,Solano AR,Lemos JR,Podestá EJ

    更新日期：2013-05-22 00:00:00

  • Mesenchymal stem cell (MSC)-mediated survival of insulin producing pancreatic β-cells during cellular stress involves signalling via Akt and ERK1/2.

    abstract：:Mesenchymal stem cells (MSC) are of interest for cell therapy since their secreted factors mediate immunomodulation and support tissue regeneration. This study investigated the direct humoral interactions between MSC and pancreatic β-cells using human telomerase-immortalized MSC (hMSC-TERT) and rat insulinoma-derived ...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2018.01.024

    authors： Liu C,Zhang W,Peradze N,Lang L,Straetener J,Feilen PJ,Alt M,Jäger C,Laubner K,Perakakis N,Seufert J,Päth G

    更新日期：2018-09-15 00:00:00

  • Regulation of corepressor alternative mRNA splicing by hormonal and metabolic signaling.

    abstract：:Alternative mRNA splicing diversifies the products encoded by the NCoR and SMRT corepressor loci. There is a programmed alteration in NCoR mRNA splicing during adipocyte differentiation from an NCoRω isoform, which contains three nuclear receptor interaction domains, to an NCoRδ isoform that contains two nuclear recep...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2015.06.036

    authors： Snyder CA,Goodson ML,Schroeder AC,Privalsky ML

    更新日期：2015-09-15 00:00:00

  • Somatostatin analogs regulate tumor corticotrophs growth by reducing ERK1/2 activity.

    abstract：:Pasireotide has been associated with tumor shrinkage in patients with Cushing's disease subjected to long term treatment. However, to date the implicated molecular mechanisms are poorly elucidated. Here, we tested pasireotide-mediated cytostatic and cytotoxic effects in ACTH-secreting primary tumor cultures and murine...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2018.12.022

    authors： Treppiedi D,Giardino E,Catalano R,Mangili F,Vercesi P,Sala E,Locatelli M,Arosio M,Spada A,Mantovani G,Peverelli E

    更新日期：2019-03-01 00:00:00