当前位置： SCI文献检索 > MOLECULAR AND CELLULAR ENDOCRINOLOGY期刊下所有文献 > Characterization and regulation of a mRNA encoding the prostaglandin F2alpha receptor in the rat ovary.

Characterization and regulation of a mRNA encoding the prostaglandin F2alpha receptor in the rat ovary.

Abstract：

:The recent cloning of several cDNAs encoding prostaglandin (PG) receptors has paved the way for a more detailed investigation of the postulated regulatory role of prostaglandins in corpus luteum function. We have utilized the reverse transcription-polymerase chain reaction (RT-PCR) to isolate a mRNA encoding the ovarian PGF(2alpha) (FP) receptor, using oligonucleotides based on the recently cloned mouse cDNA as primers. The 5'-untranslated region of the rat ovarian mRNA was isolated following 5'-RACE (rapid amplification of cDNA ends). The isolated 1526 base-pair sequence, which spans the entire open reading frame, was found 100% identical in the protein coding region to a similar sequence isolated from a rat astrocyte cDNA library, but different in the first 32 nucleotides of the 5'-untranslated region, possibly due to tissue-specific splicing heterogeneity. Using ribonuclease protection assay, a quantitative analysis of FP receptor mRNA levels was performed in corpora lutea excised from adult pseudopregnant rats (Day 8) at different timepoints (0.5-48 h) following the in vivo s.c. regimen of a luteolytic dose of the FP receptor agonist cloprostenol (5 microg). Already 3 h after cloprostenol injection, FP receptor mRNA levels exhibited a pronounced increase to values 4.0-fold higher (P < 0.01) than before injection. At 7 h through 24 h, the amount of luteal FP receptor mRNA decreased, approaching preinjection levels, whereafter they were again 3.0-fold higher (P < 0.01) at 48 h than before injection. We conclude that following homologous stimulation of the FP receptor, abundance of this mRNA is tissue-specifically regulated in a dynamic pattern, suggestive of an important role for FP receptor-mediated action on gene expression during the demise of corpus luteum function.

journal_name

Mol Cell Endocrinol

journal_title

Molecular and cellular endocrinology

authors

Olofsson JI,Leung CH,Bjurulf E,Ohno T,Selstam G,Peng C,Leung PC

doi

10.1016/0303-7207(96)03894-4

subject

Has Abstract

pub_date

1996-10-14 00:00:00

pages

45-52

issue

1

eissn

0303-7207

issn

1872-8057

pii

0303720796038944

journal_volume

123

pub_type

杂志文章

相关文献

MOLECULAR AND CELLULAR ENDOCRINOLOGY文献大全
  • Mammary Stat5 abundance and activity are not altered with lactation state in cows.

    abstract：:Stat5 is a key intracellular mediator of prolactin signalling and can activate transcription of milk proteins in response to prolactin. Therefore, in animals such as mice where lactation is dependent on prolactin, Stat5 is likely to play an important role in establishing or maintaining lactation in the mammary gland. ...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/s0303-7207(97)00161-5

    authors： Wheeler TT,Kuys YM,Broadhurst MM,Molenaar AJ

    更新日期：1997-10-20 00:00:00

  • Involvement of adenosine cyclic-3',5'-monophosphate in the signal transduction pathway of mandibular organ-inhibiting hormone of the edible crab, Cancer pagurus.

    abstract：:The juvenoid, methyl farnesoate (MF), is synthesized in the mandibular organs (MOs) of crustaceans, under the control of mandibular organ-inhibiting hormone (MO-IH). Using an in vitro assay to measure synthesis of MF by MOs, the effect of a variety of agents that affect signal transduction pathways was investigated. O...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/s0303-7207(99)00090-8

    authors： Wainwright G,Webster SG,Rees HH

    更新日期：1999-08-20 00:00:00

  • Detection of potential (anti)progestagenic endocrine disruptors using a recombinant human progesterone receptor binding and transactivation assay.

    abstract：:The present work describes the identification of (anti)progestin endocrine disrupting chemicals (EDC) using a two step screening system. In the first step a competitive binding assay was developed using recombinant human progesterone receptor (hPR). The tested chemicals were of various classes like insecticides, their...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2008.08.021

    authors： Viswanath G,Halder S,Divya G,Majumder CB,Roy P

    更新日期：2008-11-25 00:00:00

  • Dehydroepiandrosterone (DHEA) treatment in vitro inhibits adipogenesis in human omental but not subcutaneous adipose tissue.

    abstract：:Dehydroepiandrosterone (DHEA), a precursor sex steroid, circulates in sulphated form (DHEAS). Serum DHEAS concentrations are inversely correlated with metabolic syndrome components and in vivo/in vitro studies suggest a role in modulating adipose mass. To investigate further, we assessed the in vitro biological effect...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2010.02.017

    authors： Rice SP,Zhang L,Grennan-Jones F,Agarwal N,Lewis MD,Rees DA,Ludgate M

    更新日期：2010-05-14 00:00:00

  • Administration of growth hormone modulates the gene expression of basic fibroblast growth factor in rat costal cartilage, both in vivo and in vitro.

    abstract：:We examined the effect of growth hormone on local growth factor mRNA expression in male Sprague-Dawley rats. Repetitive systemic administration of growth hormone (0.4 IU every 4 h) increased the expression of IGF-I mRNA up to 2.8-fold in costal cartilage tissue compared with controls. Basic FGF (bFGF) mRNA expression ...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(95)03591-t

    authors： Izumi T,Shida J,Jingushi S,Hotokebuchi T,Sugioka Y

    更新日期：1995-07-01 00:00:00

  • Components of the anti-Müllerian hormone signaling pathway in gonads.

    abstract：:Anti-Müllerian hormone (AMH) is a member of the Transforming Growth Factor-beta (TGF-beta) family implicated in the regression of Müllerian ducts in male fetuses and in the development and function of gonads of both sexes. Members of the TGF-beta family signal through two types of serine/threonine kinase receptors cal...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章,评审

    doi：10.1016/j.mce.2003.09.005

    authors： di Clemente N,Josso N,Gouédard L,Belville C

    更新日期：2003-12-15 00:00:00

  • Dissociation of uterine eosinophilia and water imbibition from other estrogen-induced responses by nafoxidine pretreatment.

    abstract：:We investigated the effect of pretreatment of immature rats with 5 or 50 micrograms nafoxidine (UA), or with 0.05 micrograms 17 beta-estradiol (E2) on several uterine responses elicited by treatment with a test injection of 15 micrograms E2, administered 48 h after pretreatment. Early (6 h) and late (24 h) responses w...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(85)90053-x

    authors： Galand P,Tchernitchin N,Tchernitchin AN

    更新日期：1985-10-01 00:00:00

  • Receptor-selective determinants in catfish gonadotropin seat-belt loops.

    abstract：:Mammalian gonadotropins are highly selective. Charge differences between the Cys(10-11) sequence of FSHbeta and LHbeta/CGbeta seat-belt loops determine the ability of these hormones to interact with the LH-R. Selective FSH-R binding is mainly dependent on the presence of an FSHbeta-specific sequence between Cys(11-12)...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2004.06.011

    authors： Vischer HF,Marques RB,Granneman JC,Linskens MH,Schulz RW,Bogerd J

    更新日期：2004-09-30 00:00:00

  • Comparison of predicted and experimental subcellular localization of two putative rat steroid dehydrogenases from the short-chain dehydrogenase/reductase protein superfamily.

    abstract：:In the characterization of newly identified proteins, subcellular localization studies can provide important hints to the proteins' metabolic functions. Depending on the biochemical task of an enzyme, certain subcellular environmental conditions as pH or availability of cofactors and substrates have to be fulfilled. C...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2008.07.022

    authors： Keller B,Meier M,Adamski J

    更新日期：2009-03-25 00:00:00

  • Efficacy of the novel dual PI3-kinase/mTOR inhibitor NVP-BEZ235 in a preclinical model of adrenocortical carcinoma.

    abstract：:Adrenocortical cancer is a rare malignancy for which current pharmacological therapies are still insufficient. We tested the effect of a novel PI3 kinase - mammalian target of rapamycin dual inhibitor (NVP-BEZ235) on proliferation of the H295R adrenocortical cancer cell line in vitro and grown as xenografts in immunod...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2012.08.014

    authors： Doghman M,Lalli E

    更新日期：2012-11-25 00:00:00

  • Genomic organization and promoter analysis of mouse follistatin-related gene (FLRG).

    abstract：:Follistatin (FS) is well characterized as an activin-binding protein. Recently, a novel follistatin-like protein called follistatin-related gene (FLRG) that has a similar domain organization to that of follistatin has been identified. Like follistatins, FLRG binds activins and bone morphogenetic proteins (BMPs). To st...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/s0303-7207(01)00734-1

    authors： Nakatani M,Yamakawa N,Matsuzaki T,Shimasaki S,Sugino H,Tsuchida K

    更新日期：2002-03-28 00:00:00

  • Intratesticular regulation of testosterone secretion: comparison of the effects and interactions of hCG, an LHRH agonist and testicular interstitial fluid on Leydig cell testosterone secretion in vitro.

    abstract：:The stimulatory effects on Leydig cell testosterone secretion of a polypeptide(s) factor present in testicular interstitial fluid (IF) were compared with those of hCG and an LHRH agonist (LHRH-A). The actions of IF and LHRH-A were similar in showing (1) a delayed onset of action, (2) enhancement of testosterone produc...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(85)90029-2

    authors： Sharpe RM

    更新日期：1985-07-01 00:00:00

  • Expression profiling identifies Sertoli and Leydig cell genes as Fsh targets in adult zebrafish testis.

    abstract：:Spermatogonial stem cells are quiescent, undergo self-renewal or differentiating divisions, thereby forming the cellular basis of spermatogenesis. This cellular development is orchestrated by follicle-stimulating hormone (FSH), through the production of Sertoli cell-derived factors, and by Leydig cell-released androge...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2016.08.033

    authors： Crespo D,Assis LHC,Furmanek T,Bogerd J,Schulz RW

    更新日期：2016-12-05 00:00:00

  • Modulation of steroidogenic activities in testis Leydig cells.

    abstract：:Recent developments in the regulation of testicular Leydig-cell function support the following conclusions. (1) Enzymic activities involved in steroid production in the testis are mainly localized in Leydig cells. The aromatase enzyme complex for oestrogen production appears to be localized in Leydig cells as well as ...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章,评审

    doi：10.1016/0303-7207(81)90026-5

    authors： Rommerts FF,Brinkman AO

    更新日期：1981-01-01 00:00:00

  • Cytoskeletal reorganisation, 1alpha,25-dihydroxy vitamin D3 and human MG63 osteoblast maturation.

    abstract：:Bone tissue is especially receptive to physical stimulation and agents with the capacity to mimic the signalling incurred via mechanical loading on osteoblasts may find an application in a bone regenerative setting. Recently this laboratory revealed that the major serum lipid, lysophosphatidic acid (LPA), co-operated ...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2009.02.032

    authors： Mansell JP,Farrar D,Jones S,Nowghani M

    更新日期：2009-06-16 00:00:00

  • Suppression of follicular cAMP and steroid response to gonadotrophins by pretreatment with gonadotrophin.

    abstract：:This study examines the effect of preincubation of sheep ovarian follicles with hCG or FSH on the ability of isolated theca and granulosa to increase cAMP levels in response to a second incubation with hCG or FSH. Secondly, the effect of preincubation of small follicles with hCG on androstenedione and testosterone pro...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(79)90083-2

    authors： Weiss TJ,Armstrong DT

    更新日期：1979-03-01 00:00:00

  • Disposing of misfolded ER proteins: A troubled substrate's way out of the ER.

    abstract：:Secreted, plasma membrane, and resident proteins of the secretory pathway are synthesized in the endoplasmic reticulum (ER) where they undergo post-translational modifications, oxidative folding, and subunit assembly in tightly monitored processes. An ER quality control (ERQC) system oversees protein maturation and en...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章,评审

    doi：10.1016/j.mce.2019.110630

    authors： Oikonomou C,Hendershot LM

    更新日期：2020-01-15 00:00:00

  • Genome-wide methylation profiling in granulosa lutein cells of women with polycystic ovary syndrome (PCOS).

    abstract：:Polycystic Ovary Syndrome (PCOS) is the most common endocrine disorder amongst women of reproductive age, whose aetiology remains unclear. To improve our understanding of the molecular mechanisms underlying the disease, we conducted a genome-wide DNA methylation profiling in granulosa lutein cells collected from 16 wo...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2019.110611

    authors： Makrinou E,Drong AW,Christopoulos G,Lerner A,Chapa-Chorda I,Karaderi T,Lavery S,Hardy K,Lindgren CM,Franks S

    更新日期：2020-01-15 00:00:00

  • The F-domain of estrogen receptor-alpha inhibits ligand induced receptor dimerization.

    abstract：:The role of the carboxyl terminal F-domain of estrogen receptor (ERalpha) is uncertain, but evidence suggests that this region may impart internal restraint on ER dimerization in the presence of 17beta-estradiol (E2). To identify the C-terminal residues affecting human ERalpha activation, we created a series of deleti...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2008.08.001

    authors： Yang J,Singleton DW,Shaughnessy EA,Khan SA

    更新日期：2008-11-25 00:00:00

  • The anti-diabetic drug metformin inhibits vascular endothelial growth factor expression via the mammalian target of rapamycin complex 1/hypoxia-inducible factor-1α signaling pathway in ELT-3 cells.

    abstract：:The aim of this study was to elucidate whether metformin can regulate the expression of vascular endothelial growth factor (VEGF) in rat-derived uterine leiomyoma cells (ELT-3 cells). In vitro studies were conducted using ELT-3 cells. Under normoxic conditions, metformin suppressed VEGF protein levels in the supernata...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2014.08.012

    authors： Tadakawa M,Takeda T,Li B,Tsuiji K,Yaegashi N

    更新日期：2015-01-05 00:00:00

  • Metabolism of hCG and hLH to multiple urinary forms.

    abstract：:Human chorionic gonadotropin (hCG) is synthesized primarily in the placenta while human luteinizing hormone (hLH) is produced in the pituitary. Both hormones are highly homologous in structure and both appear to be altered to analogous molecular forms as the hormones are proteolytically processed, or metabolized, from...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章,评审

    doi：10.1016/s0303-7207(96)03942-1

    authors： Birken S,Kovalevskaya G,O'Connor J

    更新日期：1996-12-20 00:00:00

  • Hypospadias: interactions between environment and genetics.

    abstract：:Hypospadias is one of the most common congenital malformations. It is considered to be a mild form of the 46,XY disorders of sex development (DSD), but its precise etiology remains to be elucidated. Compromised androgen synthesis or effects can cause this frequent malformation, although the mutational analyses of the ...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章,评审

    doi：10.1016/j.mce.2011.01.006

    authors： Kalfa N,Philibert P,Baskin LS,Sultan C

    更新日期：2011-03-30 00:00:00

  • Influence of the calcium ionophores A23187 and X537A on calcitonin secretion from the isolated perfused porcine thyroid.

    abstract：:The present study examined the influence of the calcium ionophores A23187 and X537A on the calcitonin (CT) secretory process. The isolated perfused porcine thyroid was used to evaluate ionophore effects on CT secretion and thyroid slices were used to measure 45Ca uptake. Both A23187 and X537A enhanced the rate of CT r...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(86)90084-5

    authors： Pento JT

    更新日期：1986-04-01 00:00:00

  • Activated glucocorticoid receptor interacts with the INHAT component Set/TAF-Ibeta and releases it from a glucocorticoid-responsive gene promoter, relieving repression: implications for the pathogenesis of glucocorticoid resistance in acute undifferentiat

    abstract：:Set/template-activating factor (TAF)-Ibeta, part of the Set-Can oncogene product found in acute undifferentiated leukemia, is a component of the inhibitor of acetyltransferases (INHAT) complex. Set/TAF-Ibeta interacted with the DNA-binding domain of the glucocorticoid receptor (GR) in yeast two-hybrid screening, and r...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2007.10.014

    authors： Ichijo T,Chrousos GP,Kino T

    更新日期：2008-02-13 00:00:00

  • Estrogen induction of a 45 kDa secreted protein coordinately with vitellogenin in Xenopus liver.

    abstract：:In the frog Xenopus laevis, vitellogenin is the major estrogen-induced protein in the liver. We have characterized an additional secreted protein, of 45,000 Da and designated Ep45, which is also responsive to estrogen treatment. Like vitellogenin, Ep45 is not normally found in the plasma nor synthesized by the liver o...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(87)90064-5

    authors： Holland LJ,Wangh LJ

    更新日期：1987-01-01 00:00:00

  • Estrogen receptor beta signaling inhibits PDGF induced human airway smooth muscle proliferation.

    abstract：:Airway smooth muscle (ASM) cell hyperplasia driven by persistent inflammation is a hallmark feature of remodeling in asthma. Sex steroid signaling in the lungs is of considerable interest, given epidemiological data showing more asthma in pre-menopausal women and aging men. Our previous studies demonstrated that estro...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2018.04.007

    authors： Ambhore NS,Katragadda R,Raju Kalidhindi RS,Thompson MA,Pabelick CM,Prakash YS,Sathish V

    更新日期：2018-11-15 00:00:00

  • The role of the FTO (Fat Mass and Obesity Related) locus in regulating body size and composition.

    abstract：:Genomewide association studies (GWAS) have indicated that SNPs on a chromosome 16 locus encompassing FTO, as well as IRX3, 5, 6, FTM and FTL are robustly associated with human obesity. GWAS, however, are by nature gene agnostic, and SNPs reaching the appropriate statistical threshold for a given phenotype can appear a...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章,评审

    doi：10.1016/j.mce.2014.09.012

    authors： Yeo GS

    更新日期：2014-11-01 00:00:00

  • Evaluating the effects on steroidogenesis of estragole and trans-anethole in a feto-placental co-culture model.

    abstract：:In this study, we determined whether estragole and its isomer trans-anethole interfered with feto-placental steroidogenesis in a human co-culture model composed of fetal-like adrenocortical (H295R) and placental trophoblast-like (BeWo) cells. Estragole and trans-anethole are considered the biologically active compound...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2019.110583

    authors： Yancu D,Vaillancourt C,Sanderson JT

    更新日期：2019-12-01 00:00:00

  • Gonadotropin-releasing hormone overcomes follicle-stimulating hormone's inhibition of insulin-like growth factor-5 synthesis and promotion of its degradation in rat granulosa cells.

    abstract：:The effect of a gonadotropin-releasing hormone-agonist (GnRH-a) on the synthesis of insulin-like growth factor-binding protein-5 (IGFBP-5), a physiological marker for atresia, was investigated. Granulosa cells obtained from diethylstilbestrol (DES)-treated immature female rats were cultured in serum-free medium for 72...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(95)03511-5

    authors： Onoda N,Li D,Mickey G,Erickson G,Shimasaki S

    更新日期：1995-04-28 00:00:00

  • Effects of estrogens on striatal damage after 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) neurotoxicity in male and female mice.

    abstract：:Emerging evidence shows a beneficial effect of estrogens for Parkinson's disease, yet the exact potency of these compounds implicated remain obscured. In this study, we investigated the neuroprotective effect of 17beta-estradiol and estrone against 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced striatal t...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2008.07.019

    authors： Ookubo M,Yokoyama H,Takagi S,Kato H,Araki T

    更新日期：2008-12-16 00:00:00