Crystallization and preliminary X-ray analysis of papaya mosaic virus coat protein.

abstract：:We have established a time-resolved fluorescence assay to study fibrillation of the 29 residue peptide hormone glucagon under a variety of different conditions in a high-throughput format. Fibrils formed at pH 2.5 differ in fibrillation kinetics, morphology, thioflavin T staining and FTIR/CD spectra depending on salts...

journal_title：Journal of molecular biology

authors： Pedersen JS,Dikov D,Flink JL,Hjuler HA,Christiansen G,Otzen DE

更新日期：2006-01-20 00:00:00

abstract：:eIF5, a GTPase-activating protein (GAP) specific for eIF2, plays a critical role in pre-initiation complex assembly and correct AUG selection during eukaryotic translation initiation. eIF5 is involved in the formation of the multifactor complex (MFC), an important intermediate of the 43S pre-initiation complex. The C-...

journal_title：Journal of molecular biology

authors： Wei Z,Xue Y,Xu H,Gong W

更新日期：2006-05-26 00:00:00

abstract：:The GTPase Center (GAC) RNA domain in bacterial 23S rRNA is directly bound by ribosomal protein L11, and this complex is essential to ribosome function. Previous cocrystal structures of the 58-nucleotide GAC RNA bound to L11 revealed the intricate tertiary fold of the RNA domain, with one monovalent and several divale...

journal_title：Journal of molecular biology

authors： Welty R,Rau M,Pabit S,Dunstan MS,Conn GL,Pollack L,Hall KB

更新日期：2020-02-14 00:00:00

abstract：:Crystal structures of two complexes between recombinant MS2 capsids and RNA operator fragments have been determined at 2.7 A resolution. The coat protein of the RNA bacteriophage MS2 is bifunctional; it forms the icosahedral virus shell to protect the viral nucleic acid and it acts as a translational repressor by bind...

journal_title：Journal of molecular biology

authors： Valegârd K,Murray JB,Stonehouse NJ,van den Worm S,Stockley PG,Liljas L

更新日期：1997-08-01 00:00:00

abstract：:The gene-3-protein (G3P) of filamentous phage is essential for their propagation. It consists of three domains. The CT domain anchors G3P in the phage coat, the N2 domain binds to the F pilus of Escherichia coli and thus initiates infection, and the N1 domain continues by interacting with the TolA receptor. Phage are ...

journal_title：Journal of molecular biology

authors： Martin A,Schmid FX

更新日期：2003-05-09 00:00:00

abstract：:The sigma N class of sigma factors confer upon RNA polymerase the requirement for enhancer-binding activator proteins. The sigma-N (sigma N) protein of Klebsiella pneumoniae was analysed by the assay of purified peptides comprising domains or regions of sigma N defined by proteolysis or by homology alignment, respecti...

journal_title：Journal of molecular biology

authors： Cannon W,Missailidis S,Smith C,Cottier A,Austin S,Moore M,Buck M

更新日期：1995-05-12 00:00:00

abstract：:Bacterial cell shape is dictated by the cell wall, a plastic structure that must adapt to growth and division whilst retaining its function as a selectively permeable barrier. The modulation of cell wall structure is achieved by a variety of enzymatic functions, all of which must be spatially regulated in a precise ma...

journal_title：Journal of molecular biology

authors： Lovering AL,Strynadka NCJ

更新日期：2007-09-28 00:00:00

abstract：:The tetragonally arranged crystalline surface protein array (A-layer) of the fish pathogenic bacterium Aeromonas salmonicida is a virulence factor. Circular dichroism studies in the presence or absence of 0.1% sodium dodecyl sulfate showed that the secondary structure of A-protein, and its 39,439 molecular weight amin...

journal_title：Journal of molecular biology

authors： Doig P,McCubbin WD,Kay CM,Trust TJ

更新日期：1993-10-20 00:00:00

abstract：:Using a protein truncation assay, we have identified a new mutation in the neurofibromatosis type 1 (NF1) gene that causes a severe defect in NF1 pre-mRNA splicing. The mutation, which consists of a G to A transition at position +1 of the 5' splice site of exon 12a, is associated with the loss of both exons 11 and 12a...

journal_title：Journal of molecular biology

authors： Fang LJ,Simard MJ,Vidaud D,Assouline B,Lemieux B,Vidaud M,Chabot B,Thirion JP

更新日期：2001-04-13 00:00:00

abstract：:Tannins are water-soluble polyphenolic compounds in plants. Hydrolyzable tannins are derivatives of gallic acid (3,4,5-trihydroxybenzoic acid) or its meta-depsidic forms that are esterified to polyol, catechin, or triterpenoid units. Tannases are a family of esterases that catalyze the hydrolysis of the galloyl ester ...

journal_title：Journal of molecular biology

authors： Ren B,Wu M,Wang Q,Peng X,Wen H,McKinstry WJ,Chen Q

更新日期：2013-08-09 00:00:00

abstract：:The oxazolidinone family is a new class of synthetic antibiotics that bind to the bacterial 50S ribosomal subunit. Two members of the family, linezolid and XA043, were examined for their effects on translational fidelity using a lacZ reporter gene in vivo. Both promoted highly significant frameshifting and nonsense su...

journal_title：Journal of molecular biology

authors： Thompson J,O'Connor M,Mills JA,Dahlberg AE

更新日期：2002-09-13 00:00:00

abstract：:Binding of the TATA-binding protein (TBP) to the "TATA" sequences present in the promoters of eukaryotic class II genes is the first step in the sequential assembly of transcription pre-initiation complexes. Myriad structural changes, including severe bending of the DNA, accompany TBP-TATA complex formation. A detaile...

journal_title：Journal of molecular biology

authors： Parkhurst KM,Richards RM,Brenowitz M,Parkhurst LJ

更新日期：1999-06-25 00:00:00

abstract：:We have probed the surface accessibility of residues alpha187 to alpha199 of the Torpedo acetylcholine receptor with monoclonal antibody 383C, which binds uniquely to these residues. However, 383C binds to only one of the two alpha subunits in the membrane-bound receptor, neither of the two subunits in carbamylcholine...

journal_title：Journal of molecular biology

authors： Fairclough RH,Twaddle GM,Gudipati E,Lin MY,Richman DP

更新日期：1998-09-18 00:00:00

abstract：:Repeat proteins are formed from units of 20-40 aa that stack together into quasi one-dimensional non-globular structures. This modular repetitive construction means that, unlike globular proteins, a repeat protein's equilibrium folding and thus thermodynamic stability can be analysed using linear Ising models. Typical...

journal_title：Journal of molecular biology

authors： Millership C,Phillips JJ,Main ER

更新日期：2016-05-08 00:00:00

abstract：:The chloroplast thylakoid membrane of green plants is organized in stacked grana membranes and unstacked stroma membranes. We investigated the structural organization of Photosystem II (PSII) in paired grana membrane fragments by transmission electron microscopy. The membrane fragments were obtained by a short treatme...

journal_title：Journal of molecular biology

authors： Boekema EJ,van Breemen JF,van Roon H,Dekker JP

更新日期：2000-09-01 00:00:00

abstract：:In vivo, proteins occur in widely different physio-chemical environments, and, from in vitro studies, we know that protein structure can be very sensitive to environment. However, theoretical studies of protein structure have tended to ignore this complexity. In this paper, we have approached this problem by grouping ...

journal_title：Journal of molecular biology

authors： Andrade MA,O'Donoghue SI,Rost B

更新日期：1998-02-20 00:00:00

abstract：:Understanding the interaction of Arf and Hdm2 has recently become a central issue in cancer biology. In response to hyperproliferative signals, p14(Arf) stabilizes p53 by binding to Hdm2 and inhibits the ubiquitination and subsequent proteosome-dependent degradation of p53. The medical importance of the Arf-Hdm2-p53 r...

journal_title：Journal of molecular biology

authors： Bothner B,Lewis WS,DiGiammarino EL,Weber JD,Bothner SJ,Kriwacki RW

更新日期：2001-11-23 00:00:00

abstract：:Post-translational modifications are essential for the proper function of many proteins in the cell. The attachment of an isoprenoid lipid (a process termed prenylation) by protein farnesyltransferase (FTase) or geranylgeranyltransferase type I (GGTase-I) is essential for the function of many signal transduction prote...

journal_title：Journal of molecular biology

authors： Reid TS,Terry KL,Casey PJ,Beese LS

更新日期：2004-10-15 00:00:00

abstract：:The junction-resolving enzymes are a class of nucleases that introduce paired cleavages into four-way DNA junctions. They are important in DNA recombination and repair, and are found throughout nature, from eubacteria and their bacteriophages through to higher eukaryotes and their viruses. These enzymes exhibit struct...

journal_title：Journal of molecular biology

authors： White MF,Giraud-Panis MJ,Pöhler JR,Lilley DM

更新日期：1997-06-27 00:00:00

abstract：:Despite proline being assumed to be a helix-breaker, a large number of alpha-helices are found to contain Pro in globular as well as membrane proteins. Proline has no free NH group and therefore cannot form the conventional intra-helical NH.O=C hydrogen bond. An analysis of known protein structures has shown that the ...

journal_title：Journal of molecular biology

authors： Chakrabarti P,Chakrabarti S

更新日期：1998-12-11 00:00:00

abstract：:The anticancer drug daunomycin has been co-crystallized with the hexanucleotide duplex sequences d(TGTACA) and d(TGATCA) and single crystal X-ray diffraction studies of these two complexes have been carried out. Structure solution of the d(TGTACA) and d(TGATCA) complexes to 1.6 and 1.7 Angstrom resolution, respectivel...

journal_title：Journal of molecular biology

authors： Nunn CM,Van Meervelt L,Zhang SD,Moore MH,Kennard O

更新日期：1991-11-20 00:00:00

abstract：:The secondary structure of bacterial RNase P RNA, a ribozyme responsible for the maturation of the 5' end of tRNAs, is well established on the basis of sequence comparison analysis. RNase P RNA secondary structures fall into two types, A and B, which share a common core formed by the assembly of two main folding domai...

journal_title：Journal of molecular biology

authors： Massire C,Jaeger L,Westhof E

更新日期：1998-06-19 00:00:00

abstract：:We report the preparation, crystallization and preliminary X-ray crystallographic study of the Fab fragment from a heteroclitic murine (BALB/c) monoclonal anti-hen egg-white lysozyme antibody complexed with a heterologous antigen, pheasant lysozyme. The complex between the heterologous antigen and the antibody has bee...

journal_title：Journal of molecular biology

authors： Guillon V,Alzari PM,Poljak RJ

更新日期：1987-09-20 00:00:00

abstract：:A map of the 30 S ribosomal subunit is presented giving the positions of 15 of its 21 proteins. The components located in the map are S1, S3, S4, S5, S6, S7, S8, S9, S10, S11, S12, S14, S15, S18 and S20. ...

journal_title：Journal of molecular biology

authors： Ramakrishnan V,Capel M,Kjeldgaard M,Engelman DM,Moore PB

更新日期：1984-04-05 00:00:00

abstract：:Relaxation of nucleosomes on an homologous series (pBR) of ca 350-370 bp DNA minicircles originating from plasmid pBR322 was recently used as a tool to study their structure and dynamics. These nucleosomes thermally fluctuated between three distinct DNA conformations within a histone N-terminal tail-modulated equilibr...

journal_title：Journal of molecular biology

authors： Sivolob A,Lavelle C,Prunell A

更新日期：2003-02-07 00:00:00

abstract：:Multidimensional 1H, 13C and 15N nuclear magnetic resonance spectroscopy has been used to study the complex between palmitoyl-coenzyme A and acyl-coenzyme A binding protein. The 1H and the 15N spectra of the holo-protein have been almost completely assigned and so has most of the 1H spectrum of the coenzyme A part of ...

journal_title：Journal of molecular biology

authors： Kragelund BB,Andersen KV,Madsen JC,Knudsen J,Poulsen FM

更新日期：1993-04-20 00:00:00

abstract：:An analogue of the important folding intermediate of BPTI with only the disulphide bond between Cys30 and Cys51 has been characterized by 1H and 15N NMR techniques. In particular, the dynamics of the polypeptide backbone were characterized using (1H)-15N NOE and 15N T1 and T2 relaxation data. The intermediate is parti...

journal_title：Journal of molecular biology

authors： van Mierlo CP,Darby NJ,Keeler J,Neuhaus D,Creighton TE

更新日期：1993-02-20 00:00:00

abstract：:Escherichia coli pyridoxine 5'-phosphate oxidase catalyzes the terminal step in the biosynthesis of pyridoxal 5'-phosphate by the FMN oxidation of pyridoxine 5'-phosphate forming FMNH(2) and H(2)O(2). Recent studies have shown that in addition to the active site, pyridoxine 5'-phosphate oxidase contains a non-catalyti...

journal_title：Journal of molecular biology

authors： Safo MK,Musayev FN,di Salvo ML,Schirch V

更新日期：2001-07-20 00:00:00

abstract：:Elongation factor G (EF-G) is a large, five domain GTPase that catalyses the translocation of the tRNAs on the bacterial ribosome at the expense of GTP. In the crystal structure of GDP-bound EF-G, domain 1 (G domain) makes direct contacts with domains 2 and 5, whereas domain 4 protrudes from the body of the molecule. ...

journal_title：Journal of molecular biology

authors： Savelsbergh A,Matassova NB,Rodnina MV,Wintermeyer W

更新日期：2000-07-21 00:00:00

abstract：:The refined crystal structures of chicken, yeast and trypanosomal triosephosphate isomerase (TIM) have been compared. TIM is known to exist in an "open" (unliganded) and "closed" (liganded) conformation. For chicken TIM only the refined open structure is available, whereas for yeast TIM and trypanosomal TIM refined st...

journal_title：Journal of molecular biology

authors： Wierenga RK,Noble ME,Davenport RC

更新日期：1992-04-20 00:00:00