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High-resolution structure of the major periplasmic domain from the cell shape-determining filament MreC.

Abstract：

:Bacterial cell shape is dictated by the cell wall, a plastic structure that must adapt to growth and division whilst retaining its function as a selectively permeable barrier. The modulation of cell wall structure is achieved by a variety of enzymatic functions, all of which must be spatially regulated in a precise manner. The membrane-spanning essential protein MreC has been identified as the central hub in this process, linking the bacterial cytoskeleton to a variety of cell wall-modifying enzymes. Additionally, MreC can form filaments, believed to run perpendicularly to the membrane. We present here the 1.2 A resolution crystal structure of the major periplasmic domain of Streptococcus pneumoniae MreC. The protein shows a novel arrangement of two barrel-shaped domains, one of which shows homology to a known protein oligomerization motif, with the other resembling a catalytic domain from a bacterial protease. We discuss the implications of these results for MreC function, and detail the structural features of the molecule that may be responsible for the binding of partner proteins.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Lovering AL,Strynadka NCJ

doi

10.1016/j.jmb.2007.07.022

subject

Has Abstract

pub_date

2007-09-28 00:00:00

pages

1034-1044

issue

4

eissn

0022-2836

issn

1089-8638

pii

S0022-2836(07)00955-2

journal_volume

372

pub_type

杂志文章

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