Dendritic cells and their precursors isolated from human bronchoalveolar lavage: immunocytologic and functional properties.

abstract：:Identification of the genetic loci underlying asthma in humans has been hampered by variability in clinical phenotype, uncontrolled environmental influences, and genetic heterogeneity. To circumvent these complications, the genetic regulation of asthma-associated phenotypes was studied in a murine model. We characteri...

journal_title：American journal of respiratory cell and molecular biology

authors： Ewart SL,Kuperman D,Schadt E,Tankersley C,Grupe A,Shubitowski DM,Peltz G,Wills-Karp M

更新日期：2000-10-01 00:00:00

abstract：:The lung is richly supplied with peptidergic nerves that store and secrete substance P (SP), vasoactive intestinal peptide (VIP), and other neuropeptides known to potently modulate leukocyte function in vitro and airway inflammation in vivo. To investigate and characterize neuromodulation of immune responses compartme...

journal_title：American journal of respiratory cell and molecular biology

authors： Kaltreider HB,Ichikawa S,Byrd PK,Ingram DA,Kishiyama JL,Sreedharan SP,Warnock ML,Beck JM,Goetzl EJ

更新日期：1997-02-01 00:00:00

abstract：:Alveolar macrophages (AM) express gelatinase B, a member of the matrix metalloproteinase family involved in the degradation and remodeling of extracellular matrix components. We evaluated the expression of gelatinase B in the course of idiopathic pulmonary fibrosis (IPF) by studying alveolar macrophages in culture AM ...

journal_title：American journal of respiratory cell and molecular biology

authors： Lemjabbar H,Gosset P,Lechapt-Zalcman E,Franco-Montoya ML,Wallaert B,Harf A,Lafuma C

更新日期：1999-05-01 00:00:00

abstract：:Remodeling of the airways, as occurs in asthmatic patients, is associated with the continual presence of inflammatory mediators and Th2 cytokines, especially interleukin (IL)-13, during cycles of epithelial injury and repair. In this study, we examined the effect of IL-13 on well-differentiated normal human bronchial ...

journal_title：American journal of respiratory cell and molecular biology

authors： Booth BW,Adler KB,Bonner JC,Tournier F,Martin LD

更新日期：2001-12-01 00:00:00

abstract：:Single-cell RNA sequencing (scRNASeq) has advanced our understanding of lung biology, but its utility is limited by the need for fresh samples, loss of cell types by death or inadequate dissociation, and transcriptional stress responses induced during tissue digestion. Single-nucleus RNA sequencing (snRNASeq) has addr...

journal_title：American journal of respiratory cell and molecular biology

authors： Koenitzer JR,Wu H,Atkinson JJ,Brody SL,Humphreys BD

更新日期：2020-12-01 00:00:00

abstract：:A small proline-rich protein, SPR1, is overexpressed in squamous metaplasia of bronchial epithelium. We studied the expression and regulation of SPR1 in a series of human bronchial epithelial cell lines representing a model of multistep bronchial carcinogenesis. These cell lines included a primary culture of tracheobr...

journal_title：American journal of respiratory cell and molecular biology

authors： Lau D,Xue L,Hu R,Liaw T,Wu R,Reddy S

更新日期：2000-01-01 00:00:00

abstract：:Epidemiologic studies have linked gestational vitamin D deficiency to respiratory diseases, although mechanisms have not been defined. We hypothesized that antenatal vitamin D deficiency would impair airway development and alveolarization in a mouse model. We studied the effect of antenatal vitamin D deficiency by ind...

journal_title：American journal of respiratory cell and molecular biology

authors： Saadoon A,Ambalavanan N,Zinn K,Ashraf AP,MacEwen M,Nicola T,Fanucchi MV,Harris WT

更新日期：2017-03-01 00:00:00

abstract：:The human lung is constantly exposed to the environment and potential pathogens. As the interface between host and environment, the respiratory epithelium has evolved sophisticated sensing mechanisms as part of its defense against pathogens. In this review, we examine how the respiratory epithelium senses and responds...

journal_title：American journal of respiratory cell and molecular biology

authors： Benam KH,Denney L,Ho LP

更新日期：2019-03-01 00:00:00

abstract：:The mTOR pathway is one of the key signal cascades in the pathogenesis of idiopathic pulmonary fibrosis. Previous studies have mainly focused on this pathway in the fibroblasts and/or myofibroblasts, but not in the epithelial cells. In this study, we sought to investigate the role of the mTOR pathway in lung epithelia...

journal_title：American journal of respiratory cell and molecular biology

authors： Saito M,Mitani A,Ishimori T,Miyashita N,Isago H,Mikami Y,Noguchi S,Tarui M,Nagase T

更新日期：2020-06-01 00:00:00

abstract：:The cystic fibrosis transmembrane conductance regulator (CFTR) serves a pivotal role in normal epithelial homeostasis; its absence leads to destruction of exocrine tissues, including those of the gastrointestinal tract and lung. Acute regulation of CFTR protein in response to environmental stimuli occurs at several le...

journal_title：American journal of respiratory cell and molecular biology

authors： Guimbellot JS,Fortenberry JA,Siegal GP,Moore B,Wen H,Venglarik C,Chen YF,Oparil S,Sorscher EJ,Hong JS

更新日期：2008-11-01 00:00:00

abstract：:Retention kinetics for insoluble particles that deposit in the lung oftentimes resemble a multicomponent process during alveolar clearance, with each component appearing to follow simple first-order kinetics. Inasmuch as alveolar macrophages (AM) are thought to play an important role in particle removal from the lung,...

journal_title：American journal of respiratory cell and molecular biology

authors： Lehnert BE,Valdez YE,Tietjen GL

更新日期：1989-08-01 00:00:00

abstract：:Type 2 alveolar epithelial cells (AEC2) are regarded as the progenitor population of the alveolus responsible for injury repair and homeostatic maintenance. Depletion of this population is hypothesized to underlie various lung pathologies. Current models of lung injury rely on either uncontrolled, nonspecific destruct...

journal_title：American journal of respiratory cell and molecular biology

authors： Garcia O,Hiatt MJ,Lundin A,Lee J,Reddy R,Navarro S,Kikuchi A,Driscoll B

更新日期：2016-03-01 00:00:00

abstract：:The precise role of each nitric oxide (NO) synthase (NOS) isoform in the pathobiology of asthma is not well established. Our objective was to investigate the contribution of constitutive NO synthase (cNOS) and inducible NOS (iNOS) isoforms to lung mechanics and inflammatory and remodeling responses in an experimental ...

journal_title：American journal of respiratory cell and molecular biology

authors： Prado CM,Leick-Maldonado EA,Yano L,Leme AS,Capelozzi VL,Martins MA,Tibério IF

更新日期：2006-10-01 00:00:00

abstract：:The prevalence of asthma has increased in recent years, and is characterized by airway hyperresponsiveness and inflammation. Many patients report using alternative therapies to self-treat asthma symptoms as adjuncts to short-acting and long-acting β-agonists and inhaled corticosteroids (ICS). As many as 40% of patient...

journal_title：American journal of respiratory cell and molecular biology

authors： Townsend EA,Siviski ME,Zhang Y,Xu C,Hoonjan B,Emala CW

更新日期：2013-02-01 00:00:00

abstract：:Lower respiratory tract infection by respiratory syncytial virus (RSV) is a frequent cause of acute lung injury in young children and infants. Studies in adults and animals suggest that tumor necrosis factor receptor (TNFR) ligands may mediate lung injury by causing apoptosis of epithelial cells. The main goal of the ...

journal_title：American journal of respiratory cell and molecular biology

authors： Bem RA,Bos AP,Wösten-van Asperen RM,Bruijn M,Lutter R,Sprick MR,van Woensel JB

更新日期：2010-06-01 00:00:00

abstract：:Skeletal muscle dysfunction in patients with chronic obstructive pulmonary disease negatively impacts quality of life and survival. Cigarette smoking (CS) is the major risk factor for chronic obstructive pulmonary disease and skeletal muscle dysfunction; however, how CS affects skeletal muscle function remains enigmat...

journal_title：American journal of respiratory cell and molecular biology

authors： Chan SMH,Cerni C,Passey S,Seow HJ,Bernardo I,van der Poel C,Dobric A,Brassington K,Selemidis S,Bozinovski S,Vlahos R

更新日期：2020-02-01 00:00:00

abstract：:Smoking cigarettes is the major risk factor for chronic obstructive pulmonary disease (COPD). COPD is a condition associated with chronic pulmonary inflammation, characterized by macrophage activation, neutrophil recruitment, and cell injury. Many substances contained in cigarette smoke, including reactive oxygen spec...

journal_title：American journal of respiratory cell and molecular biology

authors： Facchinetti F,Amadei F,Geppetti P,Tarantini F,Di Serio C,Dragotto A,Gigli PM,Catinella S,Civelli M,Patacchini R

更新日期：2007-11-01 00:00:00

abstract：:We have determined eicosanoid production from endogenous arachidonic acid by neonatal lamb lungs stimulated with calcium ionophore A23187 during normoxia and hypoxia. Lungs of lambs 19 to 25 d of age were isolated and perfused with cell-free Krebs' bicarbonate buffer at a flow rate of 15 to 20 ml/kg/min. After 30 min ...

journal_title：American journal of respiratory cell and molecular biology

authors： Ibe BO,Isenberg WB,Raj JU

更新日期：1991-04-01 00:00:00

abstract：:The present study utilized the monocrotaline (MCT) model of pulmonary hypertension in rats to examine temporal alterations in steady-state levels of basement membrane (BM) component mRNA and deposition of protein using Northern analysis and immunohistochemistry, respectively. MCT (60 mg/kg, subcutaneous) produced sust...

journal_title：American journal of respiratory cell and molecular biology

authors： Lipke DW,Arcot SS,Gillespie MN,Olson JW

更新日期：1993-10-01 00:00:00

abstract：:Unresolved inflammation in the lung is thought to elicit loss of endothelial cell (EC) barrier integrity and impaired lung function. We have shown, in a mouse model of shock/sepsis, that neutrophil interactions with resident pulmonary cells appear central to the pathogenesis of indirect acute lung injury (iALI). Norma...

journal_title：American journal of respiratory cell and molecular biology

authors： Lomas-Neira J,Venet F,Chung CS,Thakkar R,Heffernan D,Ayala A

更新日期：2014-01-01 00:00:00

abstract：:The glucocorticoids (GC) and retinoids (RA) modulate branching morphogenesis and cytodifferentiation in the developing lung. We investigated downstream target genes that link glucocorticoid stimulation to the achievement of a mature lung in glucocorticoid receptor (GR) knockout mice. All GR(null) mice and approximatel...

journal_title：American journal of respiratory cell and molecular biology

authors： Kaplan F,Comber J,Sladek R,Hudson TJ,Muglia LJ,Macrae T,Gagnon S,Asada M,Brewer JA,Sweezey NB

更新日期：2003-01-01 00:00:00

abstract：:Hydrogen sulfide (H2S), a novel signaling gasotransmitter in the respiratory system, may have antiinflammatory properties in the lung. We examined the preventive and therapeutic effects of H2S on ozone-induced features of lung inflammation and emphysema. C57/BL6 mice were exposed to ozone or filtered air over 6 weeks....

journal_title：American journal of respiratory cell and molecular biology

authors： Li F,Zhang P,Zhang M,Liang L,Sun X,Li M,Tang Y,Bao A,Gong J,Zhang J,Adcock I,Chung KF,Zhou X

更新日期：2016-07-01 00:00:00

abstract：:Extensive tissue remodeling occurs in survivors of acute lung injury, leading to nearly normal histology and physiology in the majority of individuals, whereas others suffer significant impairment due to the development of pulmonary fibrosis. Alveolar epithelial cells play a central role in the repair process. They ar...

journal_title：American journal of respiratory cell and molecular biology

authors： Hasegawa T,Sorensen L,Dohi M,Rao NV,Hoidal JR,Marshall BC

更新日期：1997-06-01 00:00:00

abstract：:The primary function of neutrophils in host defense is to contain and eradicate invading microbial pathogens. This is achieved through a series of swift and highly coordinated responses culminating in ingestion (phagocytosis) and killing of invading microbes. While these tasks are usually performed without injury to h...

journal_title：American journal of respiratory cell and molecular biology

authors： Zemans RL,Colgan SP,Downey GP

更新日期：2009-05-01 00:00:00

abstract：:Recently, we described the isolation through fluorescent-activated cell sorting (FACS) of low autofluorescent (LAF) cells from human bronchoalveolar lavage (BAL). These LAF cells displayed an immunophenotype comparable with that of dendritic cells (DC), and showed a high potency to stimulate naive T cells. In the stud...

journal_title：American journal of respiratory cell and molecular biology

authors： van Haarst JM,Verhoeven GT,de Wit HJ,Hoogsteden HC,Debets R,Drexhage HA

更新日期：1996-12-01 00:00:00

abstract：:It had been thought that the central molecular event in the malignant transformation of a cell is the mutation of certain oncogenes-and the resultant dysregulated activation of their encoded proteins. During the past decade, however, it has become clear that alteration of the activity of the protein products of tumor ...

journal_title：American journal of respiratory cell and molecular biology

authors： Weintraub SJ

更新日期：1996-08-01 00:00:00

abstract：:In the current study, we describe cytokine and Escherichia coli lipopolysaccharide (LPS) induction of nitric oxide (NO) synthase mRNA levels in cultured smooth muscle from rat pulmonary artery (RPASM). Exposure of RPASM to interleukin-1 beta, interferon-gamma, or LPS alone did not significantly affect NO synthesis, as...

journal_title：American journal of respiratory cell and molecular biology

authors： Nakayama DK,Geller DA,Lowenstein CJ,Chern HD,Davies P,Pitt BR,Simmons RL,Billiar TR

更新日期：1992-11-01 00:00:00

abstract：:Human papillomaviruses (HPVs) such as types 6 and 11 can establish lifelong infections in airway epithelial cells in patients, and long-term infection can lead to pulmonary involvement and death. The mechanisms underlying this persistence depend on both the transcriptional activity of the viral enhancers and promoters...

journal_title：American journal of respiratory cell and molecular biology

authors： Gadi VK,Zou N,Liu JS,Cheng S,Broker TR,Sorscher EJ,Chow LT

更新日期：1999-05-01 00:00:00

abstract：:The role of guanine nucleotide-binding proteins in the induction of prostacyclin synthesis by stimulated endothelial cells is incompletely understood. We report that sodium fluoride (NaF), a potent activator of cellular guanine nucleotide-binding proteins, affected time- and concentration-dependent generation of prost...

journal_title：American journal of respiratory cell and molecular biology

authors： Garcia JG,Dominguez J,English D

更新日期：1991-08-01 00:00:00

abstract：:We have previously shown that long-term treatment of airway smooth muscle (ASM) cells with a combination of TNF-alpha and IFN-gamma impaired steroid anti-inflammatory action through the up-regulation of glucocorticoid receptor beta isoform (GRbeta) (Mol Pharmacol 2006;69:588-596). We here found that steroid actions co...

journal_title：American journal of respiratory cell and molecular biology

authors： Tliba O,Damera G,Banerjee A,Gu S,Baidouri H,Keslacy S,Amrani Y

更新日期：2008-04-01 00:00:00