当前位置： SCI文献检索 > PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA期刊下所有文献 > A scheme for sequencing large DNA molecules by identifying local nuclear-induced effects.

A scheme for sequencing large DNA molecules by identifying local nuclear-induced effects.

Abstract：

:An experimental scheme for sequencing large DNA molecules is proposed where DNA strands are replicated, with all nucleotides of a given kind marked with radioactive 32P. The marked strands are affixed to an appropriate substrate and are kept until most 32P atoms decay. The local damage caused by the decay is expected to allow the identification of the sites occupied by that particular nucleotide, using atomic scale microscopy (scanning tunneling or atomic force microscopy). Quantitative aspects and methodological considerations associated with the proposed scheme are discussed.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Kelson I,Nussinov S

doi

10.1073/pnas.91.15.6963

subject

Has Abstract

pub_date

1994-07-19 00:00:00

pages

6963-6

issue

15

eissn

0027-8424

issn

1091-6490

journal_volume

91

pub_type

杂志文章

相关文献

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA文献大全
  • Rhesus monkeys know when they remember.

    abstract：:Humans are consciously aware of some memories and can make verbal reports about these memories. Other memories cannot be brought to consciousness, even though they influence behavior. This conspicuous difference in access to memories is central in taxonomies of human memory systems but has been difficult to document i...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.071600998

    authors： Hampton RR

    更新日期：2001-04-24 00:00:00

  • Functional evidence for the mediation of diabetogenic T cell responses by HLA-A2.1 MHC class I molecules through transgenic expression in NOD mice.

    abstract：:Particular major histocompatibility complex (MHC) class II alleles clearly contribute to T cell-mediated autoimmune type 1 diabetes (T1D) in both humans and nonobese diabetic (NOD) mice. However, studies in NOD mice indicate MHC class I-restricted T cell responses are also essential to T1D development. In humans, epid...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.212221199

    authors： Marron MP,Graser RT,Chapman HD,Serreze DV

    更新日期：2002-10-15 00:00:00

  • PRMT5 modulates the metabolic response to fasting signals.

    abstract：:Under fasting conditions, increases in circulating glucagon maintain glucose balance by promoting hepatic gluconeogenesis. Triggering of the cAMP pathway stimulates gluconeogenic gene expression through the PKA-mediated phosphorylation of the cAMP response element binding (CREB) protein and via the dephosphorylation o...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1304602110

    authors： Tsai WW,Niessen S,Goebel N,Yates JR 3rd,Guccione E,Montminy M

    更新日期：2013-05-28 00:00:00

  • Knockin of mutant PIK3CA activates multiple oncogenic pathways.

    abstract：:The phosphatidylinositol 3-kinase subunit PIK3CA is frequently mutated in human cancers. Here we used gene targeting to "knock in" PIK3CA mutations into human breast epithelial cells to identify new therapeutic targets associated with oncogenic PIK3CA. Mutant PIK3CA knockin cells were capable of epidermal growth facto...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0813351106

    authors： Gustin JP,Karakas B,Weiss MB,Abukhdeir AM,Lauring J,Garay JP,Cosgrove D,Tamaki A,Konishi H,Konishi Y,Mohseni M,Wang G,Rosen DM,Denmeade SR,Higgins MJ,Vitolo MI,Bachman KE,Park BH

    更新日期：2009-02-24 00:00:00

  • Cyclin-dependent kinase 5 activates guanine nucleotide exchange factor GIV/Girdin to orchestrate migration-proliferation dichotomy.

    abstract：:Signals propagated by receptor tyrosine kinases (RTKs) can drive cell migration and proliferation, two cellular processes that do not occur simultaneously--a phenomenon called "migration-proliferation dichotomy." We previously showed that epidermal growth factor (EGF) signaling is skewed to favor migration over prolif...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1514157112

    authors： Bhandari D,Lopez-Sanchez I,To A,Lo IC,Aznar N,Leyme A,Gupta V,Niesman I,Maddox AL,Garcia-Marcos M,Farquhar MG,Ghosh P

    更新日期：2015-09-01 00:00:00

  • Frequency of DNA end joining in trans is not determined by the predamage spatial proximity of double-strand breaks in yeast.

    abstract：:DNA double-strand breaks (DSBs) are serious genomic insults that can lead to chromosomal rearrangements if repaired incorrectly. To gain insight into the nuclear mechanisms contributing to these rearrangements, we developed an assay in yeast to measure cis (same site) vs. trans (different site) repair for the majority...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1818595116

    authors： Sunder S,Wilson TE

    更新日期：2019-05-07 00:00:00

  • Multiple pathways in pressure-induced phase transition of coesite.

    abstract：:High-pressure single-crystal X-ray diffraction method with precise control of hydrostatic conditions, typically with helium or neon as the pressure-transmitting medium, has significantly changed our view on what happens with low-density silica phases under pressure. Coesite is a prototype material for pressure-induced...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1710651114

    authors： Liu W,Wu X,Liang Y,Liu C,Miranda CR,Scandolo S

    更新日期：2017-12-05 00:00:00

  • Role of an RNA cleavage/poly(A) addition site in the production of membrane-bound and secreted IgM mRNA.

    abstract：:The switch from membrane-bound to secreted IgM is accomplished by producing alternative forms of mRNA from a single mu heavy-chain gene. This process might be controlled at any of three steps--transcription termination, RNA splicing, or RNA cleavage/poly(A) addition. To distinguish between these possibilities, we have...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.82.24.8658

    authors： Danner D,Leder P

    更新日期：1985-12-01 00:00:00

  • Evolutionary conservation of key structures and binding functions of neural cell adhesion molecules.

    abstract：:The neural cell adhesion molecule N-CAM is a sialic acid-rich, cell surface glycoprotein that mediates cell adhesion by a homophilic mechanism. Its binding function has been implicated in both morphogenesis and histogenesis; during development it changes in amount at the cell surface and perinatally it undergoes a dec...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.81.21.6881

    authors： Hoffman S,Chuong CM,Edelman GM

    更新日期：1984-11-01 00:00:00

  • Regulation of binding properties of the nicotinic receptor protein by cholinergic ligands in membrane fragments from Torpedo marmorata.

    abstract：:Exposure of receptor-rich membrane fragments from Torpedo marmorata to carbamylcholine causes a slow (half-time of 5--10 min) and reversible change of properties of the cholinergic receptor protein manifested by a decrease of the initial rate of Naja nigricollis alpha-[3H]toxin binding in the presence of carbamylcholi...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.72.9.3443

    authors： Weber M,David-Pfeuty T,Changeux JP

    更新日期：1975-09-01 00:00:00

  • Floodplains as an Achilles' heel of Amazonian forest resilience.

    abstract：:The massive forests of central Amazonia are often considered relatively resilient against climatic variation, but this view is challenged by the wildfires invoked by recent droughts. The impact of such fires that spread from pervasive sources of ignition may reveal where forests are less likely to persist in a drier f...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1617988114

    authors： Flores BM,Holmgren M,Xu C,van Nes EH,Jakovac CC,Mesquita RCG,Scheffer M

    更新日期：2017-04-25 00:00:00

  • A motion area in human visual cortex.

    abstract：:We have localized an area in the human brain involved in the processing of contours defined by motion differences (kinetic contours) by comparing with positron emission tomography the regional cerebral blood flow in tasks performed with kinetic and luminance-defined gratings. These tasks included passive viewing, coun...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.92.4.993

    authors： Orban GA,Dupont P,De Bruyn B,Vogels R,Vandenberghe R,Mortelmans L

    更新日期：1995-02-14 00:00:00

  • Ergodic and nonergodic processes coexist in the plasma membrane as observed by single-molecule tracking.

    abstract：:Diffusion in the plasma membrane of living cells is often found to display anomalous dynamics. However, the mechanism underlying this diffusion pattern remains highly controversial. Here, we study the physical mechanism underlying Kv2.1 potassium channel anomalous dynamics using single-molecule tracking. Our analysis ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1016325108

    authors： Weigel AV,Simon B,Tamkun MM,Krapf D

    更新日期：2011-04-19 00:00:00

  • Design of a "green" one-step catalytic production of epsilon-caprolactam (precursor of nylon-6).

    abstract：:The ever-increasing industrial demand for nylon-6 (polycaprolactam) necessitates the development of environmentally benign methods of producing its precursor, epsilon-caprolactam, from cyclohexanone. It is currently manufactured in two popular double-step processes, each of which uses highly aggressive reagents, and e...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0506907102

    authors： Thomas JM,Raja R

    更新日期：2005-09-27 00:00:00

  • PTEN signaling through RAF1 proto-oncogene serine/threonine kinase (RAF1)/ERK in the epididymis is essential for male fertility.

    abstract：:Without a fully developed initial segment, the most proximal region of the epididymis, male infertility results. Therefore, it is important to understand the development and regulation of this crucial region. In addition to distinctively high activity levels of the components of the ERK pathway, which are essential fo...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1413186112

    authors： Xu B,Washington AM,Hinton BT

    更新日期：2014-12-30 00:00:00

  • Increase in tracheal investment with beetle size supports hypothesis of oxygen limitation on insect gigantism.

    abstract：:Recent studies have suggested that Paleozoic hyperoxia enabled animal gigantism, and the subsequent hypoxia drove a reduction in animal size. This evolutionary hypothesis depends on the argument that gas exchange in many invertebrates and skin-breathing vertebrates becomes compromised at large sizes because of distanc...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0611544104

    authors： Kaiser A,Klok CJ,Socha JJ,Lee WK,Quinlan MC,Harrison JF

    更新日期：2007-08-07 00:00:00

  • Enzymatic conversion of 5'-phosphate-terminated RNA to 5'-di- and triphosphate-terminated RNA.

    abstract：:We have isolated from vaccinia virus cores an enzyme, 5'-phosphate-polyribonucleotide kinase, that in the presence of ATP and Mg2+ catalyzes the conversion of 5'-phosphate and 5'-diphosphate termini of RNA to the 5'-triphosphate species. With the exception of dATP, other nucleoside triphosphates were inactive as phosp...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.75.10.4793

    authors： Spencer E,Loring D,Hurwitz J,Monroy G

    更新日期：1978-10-01 00:00:00

  • Brain-wide genetic mapping identifies the indusium griseum as a prenatal target of pharmacologically unrelated psychostimulants.

    abstract：:Psychostimulant use is an ever-increasing socioeconomic burden, including a dramatic rise during pregnancy. Nevertheless, brain-wide effects of psychostimulant exposure are incompletely understood. Here, we performed Fos-CreERT2-based activity mapping, correlated for pregnant mouse dams and their fetuses with amphetam...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1904006116

    authors： Fuzik J,Rehman S,Girach F,Miklosi AG,Korchynska S,Arque G,Romanov RA,Hanics J,Wagner L,Meletis K,Yanagawa Y,Kovacs GG,Alpár A,Hökfelt TGM,Harkany T

    更新日期：2019-12-17 00:00:00

  • Identification of direct residue contacts in protein-protein interaction by message passing.

    abstract：:Understanding the molecular determinants of specificity in protein-protein interaction is an outstanding challenge of postgenome biology. The availability of large protein databases generated from sequences of hundreds of bacterial genomes enables various statistical approaches to this problem. In this context covaria...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0805923106

    authors： Weigt M,White RA,Szurmant H,Hoch JA,Hwa T

    更新日期：2009-01-06 00:00:00

  • miR-146a-Traf6 regulatory axis controls autoimmunity and myelopoiesis, but is dispensable for hematopoietic stem cell homeostasis and tumor suppression.

    abstract：:microRNA-146a (miR-146a) has been previously implicated as an essential molecular brake, preventing immune overreaction and malignant transformation by attenuating NF-κB signaling, putatively via repression of the Traf6 and Irak1 genes. The exact contribution of miR-146a-mediated silencing of these genes to the contro...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1706833114

    authors： Magilnick N,Reyes EY,Wang WL,Vonderfecht SL,Gohda J,Inoue JI,Boldin MP

    更新日期：2017-08-22 00:00:00

  • Binding of the histone chaperone ASF1 to the CBP bromodomain promotes histone acetylation.

    abstract：:The multifunctional Creb-binding protein (CBP) protein plays a pivotal role in many critical cellular processes. Here we demonstrate that the bromodomain of CBP binds to histone H3 acetylated on lysine 56 (K56Ac) with higher affinity than to its other monoacetylated binding partners. We show that autoacetylation of CB...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1319122111

    authors： Das C,Roy S,Namjoshi S,Malarkey CS,Jones DN,Kutateladze TG,Churchill ME,Tyler JK

    更新日期：2014-03-25 00:00:00

  • Long-term economic growth stimulus of human capital preservation in the elderly.

    abstract：:Health care is a crucial factor in US economic growth, because growing health care costs have made US corporations less competitive than their counterparts in countries where central governments assume most of those costs. In this paper we illustrate a second, possibly more powerful, effect of health care expenditures...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0911626106

    authors： Manton KG,Gu XL,Ullian A,Tolley HD,Headen AE Jr,Lowrimore G

    更新日期：2009-12-15 00:00:00

  • Metagenome analysis of an extreme microbial symbiosis reveals eurythermal adaptation and metabolic flexibility.

    abstract：:Hydrothermal vent ecosystems support diverse life forms, many of which rely on symbiotic associations to perform functions integral to survival in these extreme physicochemical environments. Epsilonproteobacteria, found free-living and in intimate associations with vent invertebrates, are the predominant vent-associat...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0802782105

    authors： Grzymski JJ,Murray AE,Campbell BJ,Kaplarevic M,Gao GR,Lee C,Daniel R,Ghadiri A,Feldman RA,Cary SC

    更新日期：2008-11-11 00:00:00

  • Impact melting of frozen oceans on the early Earth: implications for the origin of life.

    abstract：:Without sufficient greenhouse gases in the atmosphere, the early Earth would have become a permanently frozen planet because the young Sun was less luminous than it is today. Several resolutions to this faint young Sun-frozen Earth paradox have been proposed, with an atmosphere rich in CO2 being the one generally favo...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.91.4.1248

    authors： Bada JL,Bigham C,Miller SL

    更新日期：1994-02-01 00:00:00

  • Severed corticospinal axons recover electrophysiologic control of muscle activity after x-ray therapy in lesioned adult spinal cord.

    abstract：:Mechanical injury to the adult mammalian spinal cord results in permanent loss of structural integrity at the lesion site and of the brain-controlled function distal to the lesion. Some of these consequences were permanently averted by altering the cellular constituents at the lesion site with x-irradiation delivered ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.93.20.11185

    authors： Kalderon N,Fuks Z

    更新日期：1996-10-01 00:00:00

  • A ribosome-dependent GTPase from yeast distinct from elongation factor 2.

    abstract：:Three proteins required for poly(U)-directed polyphenylalanine synthesis have been separated from yeast. Two of the factors correspond to the elongation factors 1 and 2 described for other eukaryotic systems, according to the criteria of phenylalanyl-tRNA binding and diphtheria toxin-catalyzed ADP-ribosylation. The th...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.73.1.73

    authors： Skogerson L,Wakatama E

    更新日期：1976-01-01 00:00:00

  • Glucose activates free fatty acid receptor 1 gene transcription via phosphatidylinositol-3-kinase-dependent O-GlcNAcylation of pancreas-duodenum homeobox-1.

    abstract：:The G protein-coupled free fatty acid receptor-1 (FFA1/GPR40) plays a major role in the regulation of insulin secretion by fatty acids. GPR40 is considered a potential therapeutic target to enhance insulin secretion in type 2 diabetes; however, its mode of regulation is essentially unknown. The aims of this study were...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1114350109

    authors： Kebede M,Ferdaoussi M,Mancini A,Alquier T,Kulkarni RN,Walker MD,Poitout V

    更新日期：2012-02-14 00:00:00

  • Caspase 3 activity is required for skeletal muscle differentiation.

    abstract：:The cellular alterations associated with skeletal muscle differentiation share a high degree of similarity with key phenotypic changes usually ascribed to apoptosis. For example, actin fiber disassembly/reorganization is a conserved feature of both apoptosis and differentiating myoblasts and the conserved muscle contr...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.162172899

    authors： Fernando P,Kelly JF,Balazsi K,Slack RS,Megeney LA

    更新日期：2002-08-20 00:00:00

  • Myofibrillogenesis visualized in living embryonic cardiomyocytes.

    abstract：:Myofibril formation was visualized in cultured live cardiomyocytes that were transfected with plasmids expressing green fluorescent protein (GFP) linked to the Z-band protein, alpha-actinin. The expression of this fluorescent protein provided an in vivo label for structures containing alpha-actinin. The GFP-alpha-acti...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.94.17.9493

    authors： Dabiri GA,Turnacioglu KK,Sanger JM,Sanger JW

    更新日期：1997-08-19 00:00:00

  • A new form of cerebellar long-term potentiation is postsynaptic and depends on nitric oxide but not cAMP.

    abstract：:Long-term depression (LTD) at cerebellar parallel fiber (PF)-Purkinje cell synapses must be balanced by long-term potentiation (LTP) to prevent saturation and allow reversal of motor learning. The only previously analyzed form of cerebellar LTP is induced by 4-8 Hz PF stimulation and requires cAMP but not nitric oxide...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.122206399

    authors： Lev-Ram V,Wong ST,Storm DR,Tsien RY

    更新日期：2002-06-11 00:00:00