Abstract:
:The RNA polymerase II large subunit contains an essential carboxy-terminal domain (CTD) believed to be involved in the response to regulators during transcription initiation. The CTD is phosphorylated on a portion of RNA polymerase II molecules in vivo and it can be phosphorylated by the general transcription factor TFIIH in vitro. A highly purified TFIIH from rat liver has been described; this, like human and yeast TFIIH, contains associated CTD kinase and helicase activities. We report here that two polypeptides of the purified mammalian TFIIH are the MO15/Cdk7 kinase and cyclin H subunits of the Cdk-activating kinase Cak, previously identified as a positive regulator of Cdc2 and Cdk2. TFIIH and Cak preparations are each capable of phosphorylating recombinant CTD and recombinant Cdk2 proteins. The presence of Cak in TFIIH indicates that Cak may have roles in transcriptional regulation and in cell-cycle control.
journal_name
Naturejournal_title
Natureauthors
Serizawa H,Mäkelä TP,Conaway JW,Conaway RC,Weinberg RA,Young RAdoi
10.1038/374280a0subject
Has Abstractpub_date
1995-03-16 00:00:00pages
280-2issue
6519eissn
0028-0836issn
1476-4687journal_volume
374pub_type
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