Abstract：
OBJECTIVE:To assess the effect of human recombinant leukemia inhibitory factor in different doses on human blastocyst formation. SETTING:A university-based tertiary referral center (The Toronto Hospital). INTERVENTIONS:Nontransferable human embryos (n = 473) at the two- to six-cell stage were obtained from patients undergoing IVF and were split randomly into five groups. Embryos in group A (n = 164) were cultured as the control group in Ham's F-10 (GIBCO-BRL, Grand Island, NY) + 10% human sera. Embryos in groups B, C, D, and E (n = 54, 78, 87, and 80, respectively) were cultured in the same medium supplemented with human recombinant leukemia inhibitory factor in four different concentrations (5, 7.5, 10, and 20 ng/mL, respectively). Morphological assessment of embryo development was recorded daily. MAIN OUTCOME MEASURE:Human blastocyst formation. RESULTS:No significant difference was detected in the rate of blastocyst formation of embryos in the study groups when compared with embryos in group A. CONCLUSIONS:This study shows that 5 to 20 ng/mL of recombinant leukemia inhibitory factor in standard medium does not enhance in vitro human blastocyst formation. It is possible that recombinant leukemia inhibitory factor may play a role at later stages of human embryogenesis and during implantation.
journal_name
Fertil Steriljournal_title
Fertility and sterilityauthors
Jurisicova A,Ben-Chetrit A,Varmuza SL,Casper RFsubject
Has Abstractpub_date
1995-11-01 00:00:00pages
999-1002issue
5eissn
0015-0282issn
1556-5653pii
S0015-0282(16)57918-7journal_volume
64pub_type
杂志文章abstract：OBJECTIVE:To demonstrate the feasibility of studying exosomes directly from peritoneal fluid, we isolated exosomes from endometriosis patient samples and from controls, and characterized their cargo. DESIGN:Case-control experimental study. SETTING:Academic clinical center. PATIENT (S):Women with and without endometr...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2019.09.032
更新日期：2020-02-01 00:00:00
abstract：OBJECTIVE:To compare two commonly used protocols (fresh vs. vitrified) used to transfer euploid blastocysts after IVF with preimplantation genetic screening. DESIGN:Randomized controlled trial. SETTING:Private assisted reproduction center. PATIENT(S):A total of 179 patients undergoing IVF treatment using preimplanta...
journal_title：Fertility and sterility
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.fertnstert.2016.12.022
更新日期：2017-03-01 00:00:00
abstract：OBJECTIVE:To evaluate the place of ultrasound-directed transvaginal transmyometrial ET in a protocol using both the transcervical and transmyometrial routes in a step-wise fashion. DESIGN:A prospective descriptive clinical study. SETTING:A university-based assisted conception unit. PATIENTS:Thirteen patients who had...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：
更新日期：1996-05-01 00:00:00
abstract：:The acrosome reaction of human spermatozoa incubated in the presence or absence of vested human oocytes was investigated. All gametes were obtained from human in vitro fertilization (IVF) cases. Spermatozoa were collected after incubation in insemination medium only and following removal of the oocytes from inseminati...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)60379-5
更新日期：1988-12-01 00:00:00
abstract：OBJECTIVE:To determine whether patients support a mandatory single blastocyst transfer (mSBT) policy in IVF. DESIGN:Prospective survey study. SETTING:Academic hospital-based infertility center. PATIENT(S):Two hundred sixty-two female patients presenting for fresh or cryopreserved/thawed ET after IVF. INTERVENTION(S...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2011.07.1140
更新日期：2011-10-01 00:00:00
abstract：OBJECTIVE:To investigate differentially expressed genes in epithelial and stromal cells of eutopic endometrium from patients with deep endometriosis and women with normal pelvic cavities using laser capture microdissection and complementary DNA microarrays. DESIGN:Prospective study. SETTING:University hospital. PATI...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2005.04.041
更新日期：2005-10-01 00:00:00
abstract：:A randomized, prospective study was conducted to compare ovarian stimulation with human menopausal gonadotropin (hMG) and human follicle-stimulating hormone (hFSH) in an in vitro fertilization and embryo transfer (IVF-ET) program. Minimal inclusion criteria included age less than or equal to 37, tubal infertility, reg...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/s0015-0282(16)60011-0
更新日期：1988-07-01 00:00:00
abstract：OBJECTIVE:To investigate the effects of P, medroxyprogesterone acetate (MPA), and dydrogesterone (DYD) and its metabolite, 20-alpha-dihydrodydrogesterone (DHD) on endothelial synthesis of nitric oxide (NO) and characterize the signaling events recruited by these compounds. The Women's Health Initiative trial reports an...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2006.05.018
更新日期：2006-10-01 00:00:00
abstract：:After long-term estradiol deficiency, female rats displayed body mass gain accompanied by an increase in the size of adipocytes, an increase in hyperglycemia, and a decrease in insulinemia. The effects were reversed by daily estradiol treatment. Adiposity was suggested by the increased vascular endothelial growth fact...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2011.01.020
更新日期：2011-03-15 00:00:00
abstract：:Combined therapy with clomiphene and intranasal luteinizing hormone-releasing hormone (LHRH) was used to induce ovulation in eight chronically anovulatory patients under treatment for infertility. Clomiphene, 100 mg daily, was given from the 5th to the 9th day of the cycle. Synthetic LHRH was administered intranasally...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：
更新日期：1980-11-01 00:00:00
abstract：OBJECTIVE:To examine the small antral follicle (SAF) cohort in ovaries of adult rhesus monkeys after consumption of a Western-style diet (WSD), with or without chronically elevated androgen levels since before puberty. DESIGN:Cholesterol or T (n = 6 per group) implants were placed SC in female rhesus macaques beginnin...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2015.11.045
更新日期：2016-04-01 00:00:00
abstract：OBJECTIVE:To assess insulin and insulin-like growth factor I (IGF-I) action in women with polycystic ovarian syndrome (PCOS). DESIGN:Hyperinsulinemia was determined by measuring the insulin responses during a 2-hour oral glucose tolerance test (OGTT). Quantification of in vivo insulin action was determined by a freque...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：
更新日期：1992-04-01 00:00:00
abstract：OBJECTIVE:To describe a live birth obtained in Italy after autologous orthotopic transplantation of cryopreserved ovarian cortical tissue. DESIGN:Case report. SETTING:University department of gynecology and obstetrics, reproductive medicine and IVF unit. PATIENT(S):A 29-year-old patient affected by β-thalassemia (in...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2012.09.029
更新日期：2013-01-01 00:00:00
abstract：OBJECTIVE:To assess the risk of cervical neoplasia associated with the use of ovulation-inducing agents such as clomiphene citrate (CC) DESIGN: Case-cohort study. SETTING:Infertility clinics in Seattle, Washington. PATIENTS:A cohort of 3,837 women evaluated for infertility at some time during 1974-1985. MAIN OUTCOME...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：
更新日期：1996-01-01 00:00:00
abstract：OBJECTIVE:To study the differences in the cleavage time between types of embryo chromosomal abnormalities and elaborate algorithm to exclude aneuploid embryos according to the likelihood to be euploid. DESIGN:Retrospective cohort study. SETTING:University affiliated private center. PATIENT(S):Preimplantational genet...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2016.09.019
更新日期：2017-01-01 00:00:00
abstract：OBJECTIVE:To evaluate vascular perfusion and uterine healing after laparoscopic myomectomy using three-dimensional power Doppler ultrasound (3D-PDU). DESIGN:Prospective study. SETTING:University-affiliated hospital. PATIENT(S):Ninety-seven women with symptomatic myomas warranting laparoscopic myomectomy. INTERVENTI...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章
doi：10.1016/j.fertnstert.2008.07.1771
更新日期：2009-09-01 00:00:00
abstract：:On the basis of studies in monkeys, the hypothesis has been proposed that vasectomy induces the formation of circulating immune complexes (CICs), which--via activation of the complement system--may result in immune injury of the endothelium and thereby exacerbate atherosclerosis; the same mechanism has been suggested ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：
更新日期：1982-06-01 00:00:00
abstract：:Chronic treatment with highly active analogs of luteinizing hormone (LH)-releasing hormone (LH-RH) induces "paradoxical" antifertility effects. Intact male rats, a reduction in testosterone production is observed after the administration of [D-Ser(But)6]-LH-RH(1-9)ethylamide (burserelin, Hoe 766), 50 ng/day subcutaneo...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)43461-8
更新日期：1978-08-01 00:00:00
abstract：OBJECTIVE:To evaluate follicular FSH and LH requirements during suppression of endogenous gonadotropins with the GnRH antagonist Nal-Glu and whether LH-like activity could be supplied by administering subcutaneous hCG. DESIGN:Randomized clinical trial. PARTICIPANTS:Thirty-two normally cycling females in the late foll...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章,随机对照试验
doi：
更新日期：1995-02-01 00:00:00
abstract：OBJECTIVE:To examine compliance with ethical guidelines of the American Society for Reproductive Medicine (ASRM) regarding trait-based payment variation, presentation of risks, and minimum recruitment age. DESIGN:In June 2010, we systematically examined 207 websites, of which 102 were egg donor agency or IVF clinic we...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2012.06.052
更新日期：2012-10-01 00:00:00
abstract：:In 6-day pregnant New Zealand White rabbits dosed intravenously with 3H-nicotine, the 3H-activity in the uterine fluid was approximately 5 to 11 times greater than that in the plasma at the corresponding times; 3H-nicotine itself accounted for most of this radioactivity. Dichlorodiphenyltrichloroethane (DDT) also accu...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：
更新日期：1976-10-01 00:00:00
abstract：OBJECTIVE:To evaluate by serial ultrasound scans how long a Ringer's lactate instillation remains in the peritoneal cavity after laparoscopy. DESIGN:Prospective, randomized, double blind, clinical trial. SETTING:Tertiary care, university hospital. PATIENT(S):Thirty-six patients undergoing diagnostic or minor operati...
journal_title：Fertility and sterility
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.fertnstert.2005.01.111
更新日期：2005-07-01 00:00:00
abstract：:The Hammen semen quality classification system is a fertility index with six qualities based on sperm count, assessment of sperm motility, number of immotile spermatozoa, number of abnormal sperm heads, and sperm viability. It was developed by Hammen during the 1940s. In the present study, the fertility of 1077 men cl...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)45624-4
更新日期：1981-07-01 00:00:00
abstract：OBJECTIVE:To compare the expression of HOXA-10 and E-cadherin in the endometrium of women with recurrent implantation failure (RIF), women with recurrent miscarriage (RM), and women with proven fertility (normal control; NC). DESIGN:Observational cohort study. SETTING:University assisted reproductive unit. PATIENT(S...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2016.09.016
更新日期：2017-01-01 00:00:00
abstract：:A recent survey of American Society for Reproductive Medicine (ASRM) members indicates that they express a commitment to professional standards and a desire for advocacy. Their attitudes echo traditional values of professionalism, upon which ASRM should capitalize. ...
journal_title：Fertility and sterility
pub_type： 评论,杂志文章
doi：10.1016/j.fertnstert.2004.01.052
更新日期：2004-09-01 00:00:00
abstract：OBJECTIVE:To study the in vivo mechanisms of action of ulipristal acetate (UPA) on uterine myomas. DESIGN:Retrospective histologic and immunohistochemical (IHC) study of myomas. SETTING:Academic research unit. PATIENT(S):Among 59 women with symptomatic myomas who underwent myomectomy, 42 were treated preoperatively ...
journal_title：Fertility and sterility
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.fertnstert.2015.04.025
更新日期：2015-08-01 00:00:00
abstract：:Cell proliferation of endometrial stromal cells treated with peritoneal fluid of women (aged 25 to 43 years) with endometriosis (n = 12) statistically significantly increased compared with the control treatment (peritoneal fluid of women without endometriosis, n = 8). Also, COX-2 gene expression and prostaglandin E(2)...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2010.11.039
更新日期：2011-04-01 00:00:00
abstract：:Nonaromatizable androgens, administered in high doses to an adult patient with partial androgen insensitivity, failed to result in a change in phallic size despite a clear decline in SHBG and gonadotropin levels. These findings raise the question of differential tissue sensitivity to androgens. Because ancillary labor...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)56114-7
更新日期：1993-08-01 00:00:00
abstract：:The International Committee for Monitoring Assisted Reproductive Technology's 7th World Report for the year 2000 analyzes wide variations in live and multiple birth rates from 49 countries and six regions by type of assisted reproductive technology, age, number of embryos transferred, and multiple births. More than 46...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2006.01.011
更新日期：2006-06-01 00:00:00
abstract：OBJECTIVE:To assess the use and effectiveness of IVF in a cohort of women undergoing surgery for endometriosis. DESIGN:Cohort study. SETTING:University hospital. PATIENT(S):Four hundred thirty-eight patients who attempted to become pregnant after conservative surgery for endometriosis. INTERVENTION(S):Interview. M...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2008.02.004
更新日期：2009-05-01 00:00:00
