Effect of transforming growth factor-beta 1 on the insulin-like growth factor system in cultured porcine Leydig cells.

abstract：:Glucose transport activity ([3H]D-glucose uptake) in liver sinusoidal membrane vesicles (SMVs) from hyperthyroid rats was significantly higher than that from euthyroid controls (2.1-times increase in V(max) with K(m) unchanged at approximately 18 mM), associated with increased GLUT2 expression. In contrast, glucose tr...

journal_title：Molecular and cellular endocrinology

authors： Kemp HF,Hundal HS,Taylor PM

更新日期：1997-04-04 00:00:00

abstract：:Four abundant cDNA clones have been isolated from a rat epididymal cDNA library. Northern blot analysis has shown that these clones partially encode 4.5 kb, 2.8 kb, 1.2 kb and 0.85 kb mRNAs and that their expression is not detectable in total RNA preparations from heart, kidney, liver or testis. Fourteen days after ca...

journal_title：Molecular and cellular endocrinology

authors： Walker JE,Jones R,Moore A,Hamilton DW,Hall L

更新日期：1990-11-12 00:00:00

abstract：:Zinc ions influence adipose tissue metabolism by regulating leptin secretion and by promoting free fatty acid release and glucose uptake. The mechanisms controlling zinc metabolism in adipose tissue are unknown. We therefore examined the gene-expression levels of a number of zinc-transporting proteins in adipose tissu...

journal_title：Molecular and cellular endocrinology

authors： Smidt K,Pedersen SB,Brock B,Schmitz O,Fisker S,Bendix J,Wogensen L,Rungby J

更新日期：2007-01-29 00:00:00

abstract：:Many agrochemicals have endocrine disrupting properties. A subset of these chemicals is characterized as "estrogenic". In this review, we describe several distinct ways that chemicals used in crop production can affect estrogen signaling. Using three agrochemicals as examples (DDT, endosulfan, and atrazine), we illust...

journal_title：Molecular and cellular endocrinology

authors： Vandenberg LN,Najmi A,Mogus JP

更新日期：2020-12-01 00:00:00

abstract：:The transcriptional activity of various lengths of the 5'-untranslated region (UTR) of the murine LH receptor (R) gene were studied using the luciferase reporter gene in transiently transfected mouse Leydig tumor cells (mLTC-1). Chinese hamster ovarian (CHO) and HeLa cells were used as controls. The basal transcriptio...

journal_title：Molecular and cellular endocrinology

authors： El-Hefnawy T,Krawczyk Z,Nikula H,Viherä I,Huhtaniemi I

更新日期：1996-05-31 00:00:00

abstract：:The role of protein kinase C (PKC) isoforms (PKCs) in GnRH-stimulated MAPK [ERK1/2, JNK1/2 and p38) phosphorylation was examined in gonadotrope derived cells. GnRH induced a protracted activation of ERK1/2 and a slower and more transient activation of JNK1/2 and p38MAPK. Gonadotropes express conventional PKCα and PKCβ...

journal_title：Molecular and cellular endocrinology

authors： Mugami S,Dobkin-Bekman M,Rahamim-Ben Navi L,Naor Z

更新日期：2018-03-05 00:00:00

abstract：:Resistance to thyroid hormone can be due to heterozygous, dominant negative (DN) THRA (RTHα) or THRB (RTHβ) mutations, but the underlying mechanisms are incompletely understood. Here, we delineate the spatiotemporal expression of TH receptors (TRs) in zebrafish and generated morphants expressing equivalent amounts of ...

journal_title：Molecular and cellular endocrinology

authors： Marelli F,Carra S,Agostini M,Cotelli F,Peeters R,Chatterjee K,Persani L

更新日期：2016-03-15 00:00:00

abstract：:The effects of a GnRH antagonist analogue (N-acetyl-Ala1,D-p-Cl-Phe2,D-Trp3,6-GnRH, Ant.) and a GnRH antiserum (A/S) on the development of pituitary-testicular function were studied in immature (23/24-31/32-day-old) rats. In another experiment the Ant. treatment was combined with bromocriptine (BR)-induced hypoprolact...

journal_title：Molecular and cellular endocrinology

authors： Huhtaniemi IT,Stewart JM,Channabasavaiah K,Fraser HM,Clayton RN

更新日期：1984-02-01 00:00:00

abstract：:Diabetes-induced neutrophil NETosis impairs wound healing through neutrophil extracellular traps (NETs). Reactive oxygen species (ROS)-triggered activation of mitogen-activated protein kinase (MAPK) ERK1/2 and p38 is involved in NETosis. Hydrogen sulfide (H2S), an endogenous signaling molecule, accelerates diabetic wo...

journal_title：Molecular and cellular endocrinology

authors： Yang CT,Chen L,Chen WL,Li N,Chen MJ,Li X,Zheng X,Zhao YZ,Wu YX,Xian M,Liu J

更新日期：2019-01-15 00:00:00

abstract：:Inhibitor-1 following phosphorylation by protein kinase A inhibits phosphoprotein phosphatase-1. We have found that in the rat heart inhibitor-1 is present only in the cytosolic fraction and that its phosphorylation in ventricular slices was increased by isoproterenol but not by isoproterenol and propranolol together....

journal_title：Molecular and cellular endocrinology

authors： Iyer RB,Koritz SB,Kirchberger MA

更新日期：1988-01-01 00:00:00

abstract：:1,25-Dihydroxyvitamin D(3) (1,25D(3)), the active metabolite of vitamin D(3), inhibits breast cancer cell growth in vivo and in vitro. To examine mechanisms of 1,25D(3) induced growth arrest and apoptosis, cell lines were established from DMBA induced mammary tumors derived from vitamin D(3) receptor knockout (VDRKO) ...

journal_title：Molecular and cellular endocrinology

authors： Zinser GM,McEleney K,Welsh J

更新日期：2003-02-28 00:00:00

abstract：:Peripheral benzodiazepine (BZ) binding sites (PBzS) were characterized on placental explant membranes. [3H]PK 11195, an isoquinoline carboxamide derivative, which is a ligand specific for PBzS, labeled these sites with an equilibrium dissociation constant of 2.1 nM; the maximal number of binding sites was 396 fmol/mg ...

journal_title：Molecular and cellular endocrinology

authors： Barnea ER,Fares F,Gavish M

更新日期：1989-07-01 00:00:00

abstract：:Fibroblast growth factor (FGF)-21 is an endocrine member of the FGF family with healthy effects on glucose and lipid metabolism. FGF21 reduces glycemia and lipidemia in rodent models of obesity and type 2 diabetes. In addition to its effects improving insulin sensitivity, FGF21 causes weight loss by increasing energy ...

journal_title：Molecular and cellular endocrinology

authors： Giralt M,Gavaldà-Navarro A,Villarroya F

更新日期：2015-12-15 00:00:00

abstract：:1,25-Dihydroxyvitamin D (1,25(OH)(2)D) plays a critical role in calcium and phosphorus (Pi) metabolism, bone growth, and tissue differentiation. The synthesis of 1,25(OH)(2)D in the proximal renal tubule is the primary determinant of its circulating concentration and is mediated by the mitochondrial enzyme, 25-hydroxy...

journal_title：Molecular and cellular endocrinology

authors： Perwad F,Portale AA

更新日期：2011-12-05 00:00:00

abstract：:The aim of this study is to further characterize the prolactin receptors (PRL-R) previously reported in the murine Leydig tumor MA-10 cell line, as well as to study their homologous and heterologous regulation. Two forms of PRL-R, a high and a low molecular weight form, were revealed by studies of covalent crosslinkin...

journal_title：Molecular and cellular endocrinology

authors： Weiss-Messer E,Ber R,Amit T,Barkey RJ

更新日期：1998-08-25 00:00:00

abstract：:The effects of calcitonin (CT) on the cellular transport and accumulation of Ca were studied in bone cells grown in primary culture. The following sequence of events was detected: (1) After 1--2 h of incubation with CT the initial rate of Ca influx into bone cells transiently increased. A longer incubation with CT (3-...

journal_title：Molecular and cellular endocrinology

authors： Eilam Y,Szydel N,Harell A

更新日期：1980-06-01 00:00:00

abstract：:In type 2 diabetes, a strong correlation between intramyocellular lipid accumulation and insulin resistance exists but whether intramyocellular accumulation is a cause or a consequence of insulin resistance is not clear. Lipid accumulation and response to insulin were evaluated in primary human myotubes derived from n...

journal_title：Molecular and cellular endocrinology

authors： Aguer C,Mercier J,Kitzmann M

更新日期：2010-02-05 00:00:00

abstract：:The interaction between thyroid microsomal autoantibodies and thyroid microsomal antigen/thyroid peroxidase (TPO) has been studied using both intact antigen preparations and their water-soluble trypsin fragments. In an analysis of sera from 30 patients with Graves' or Hashimoto's diseases, microsomal antibodies showed...

journal_title：Molecular and cellular endocrinology

authors： Nakajima Y,Howells RD,Pegg C,Jones ED,Smith BR

更新日期：1987-09-01 00:00:00

abstract：:The family of G protein-coupled receptors (GPCRs) is the largest class of membrane proteins and an important drug target due to their role in many (patho)physiological processes. Besides small molecules, GPCRs can be targeted by biologicals including antibodies and antibody fragments. This review describes the use of ...

journal_title：Molecular and cellular endocrinology

authors： De Groof TWM,Bobkov V,Heukers R,Smit MJ

更新日期：2019-03-15 00:00:00

abstract：:Growth hormone (GH) secretion, evoked by either pituitary adenylate cyclase-activating polypeptide (PACAP) or dopamine (DA), is dependent on both voltage-sensitive calcium channels (VSCC) and cAMP signaling in goldfish. We further characterized the involvement of Ca2+ in evoked release by PACAP and DA, by examining th...

journal_title：Molecular and cellular endocrinology

authors： Chang JP,Wong CJ,Davis PJ,Soetaert B,Fedorow C,Sawisky G

更新日期：2003-08-29 00:00:00

abstract：:Ghrelin is a peptide hormone that was originally isolated from the stomach as the endogenous ligand for the growth hormone secretagogue receptor (GHSR). Ghrelin has many functions, including the regulation of appetite and gut motility, growth hormone release from the anterior pituitary and roles in the cardiovascular ...

journal_title：Molecular and cellular endocrinology

authors： Chopin L,Walpole C,Seim I,Cunningham P,Murray R,Whiteside E,Josh P,Herington A

更新日期：2011-06-20 00:00:00

abstract：:We have investigated protein kinase C (PKC) regulation by 1,25-(OH)2D3 in the rat osteosarcoma cell line ROS 17/2.8 since previous reports have implicated PKC in the 1,25-(OH)2D3-mediated regulation of osteocalcin gene expression (J. Biol. Chem. 267 (1992) 12562; Endocrinology 136 (1995) 5685). Here we report that 1,2...

journal_title：Molecular and cellular endocrinology

authors： Rivera-Bermúdez MA,Bertics PJ,Albrecht RM,Mosavin R,Mellon WS

更新日期：2002-02-25 00:00:00

abstract：:It has recently been reported that phenol red, a pH indicator present in most tissue culture media, is a weak estrogen that can stimulate some estrogen-sensitive cells. However, the relative impact of phenol red on various cell lines is controversial. We examined the effect of phenol red on several estrogen-responsive...

journal_title：Molecular and cellular endocrinology

authors： Welshons WV,Wolf MF,Murphy CS,Jordan VC

更新日期：1988-06-01 00:00:00

abstract：:Granulosa cells of small follicles differentiate in vitro in serum-free medium, resulting in increased estradiol secretion and abundance of mRNA encoding cytochrome P450aromatase (P450arom). We tested the hypothesis that differentiation in vitro also involves increased expression of 3beta- and 17beta-hydroxysteroid de...

journal_title：Molecular and cellular endocrinology

authors： Sahmi M,Nicola ES,Silva JM,Price CA

更新日期：2004-08-31 00:00:00

abstract：:Northern blot analysis of total RNA from a variety of rabbit tissues indicated that placenta is the primary site of expression of the protein hormone relaxin (previously called SQ10) in rabbits. Relaxin was not detected by this method in other rabbit tissues, including normal trachea and several squamous tissues. Howe...

journal_title：Molecular and cellular endocrinology

authors： Bernacki SH,Medvedev A,Holloway G,Dawson M,Lotan R,Jetten AM

更新日期：1998-03-16 00:00:00

abstract：:The aim of this study was to elucidate whether metformin can regulate the expression of vascular endothelial growth factor (VEGF) in rat-derived uterine leiomyoma cells (ELT-3 cells). In vitro studies were conducted using ELT-3 cells. Under normoxic conditions, metformin suppressed VEGF protein levels in the supernata...

journal_title：Molecular and cellular endocrinology

authors： Tadakawa M,Takeda T,Li B,Tsuiji K,Yaegashi N

更新日期：2015-01-05 00:00:00

abstract：:Prostate cancer (PCa) is a major health issue in the Western world. Current clinical imperatives for this disease include better stratification of indolent versus aggressive disease to enable improved patient management, as well as the identification of more effective therapies for the prevention and treatment of meta...

journal_title：Molecular and cellular endocrinology

authors： Das R,Feng FY,Selth LA

更新日期：2019-01-15 00:00:00

abstract：:We investigated the involvement of protein synthesis in the stimulatory action of thyroid hormones on amino acid accumulation and characterized K(+) currents involved in the hyperpolarizing effect of thyroxine (T(4)) on Sertoli cells. Immature rat testes were incubated in Krebs Ringer-bicarbonate buffer (KRb) in the p...

journal_title：Molecular and cellular endocrinology

authors： Menegaz D,Zamoner A,Royer C,Leite LD,Bortolotto ZA,Silva FR

更新日期：2006-02-26 00:00:00

abstract：:Transforming growth factor beta (TGF-beta) is a member of a family of cytokines that regulate differentiation and proliferation in a wide variety of tissues including the pituitary gland. In both the normal pituitary and tumorous cell lines TGF-beta1 has anti-proliferative activity, however the intracellular mechanism...

journal_title：Molecular and cellular endocrinology

authors： Frost SJ,Simpson DJ,Farrell WE

更新日期：2001-05-15 00:00:00

abstract：:Adipose tissue-derived adipokines play important roles as regulators of skeletal growth and development. CTRP1, a paralog of adiponectin, is a member of the C1q and tumor necrosis factor (TNF)-related protein (CTRP) superfamily. It is expressed at high levels in adipose tissue and has recently emerged as a novel adipo...

journal_title：Molecular and cellular endocrinology

authors： Akiyama H,Otani M,Sato S,Toyosawa S,Furukawa S,Wakisaka S,Maeda T

更新日期：2013-04-30 00:00:00