Abstract:
:Concentration of factor VIII from fresh plasma by cryoprecipitation remains the basis for preparation of products used to treat haemophilia A. This paper describes the preparation of a factor VIII concentrate from small plasma pools in transfusion centres with drying facilities. The dried concentrate from one litre of plasma dissolves very well in 50 or 100 ml of distilled water and contains around 500 IU per bottle. The specific activity per mg protein is 0.19 IU and the fibrinogen concentration is half that in frozen cryoprecipitate. This method of drying causes no appreciable loss in the factor VIIIC activity and little denaturation as shown by the factor-VIII-related antigen/factor VIIIC ratio of 1.7.
journal_name
J Clin Patholjournal_title
Journal of clinical pathologyauthors
Millgan G,Graham R,Hanratty S,Muir W,Mitchell Rdoi
10.1136/jcp.34.10.1091subject
Has Abstractpub_date
1981-10-01 00:00:00pages
1091-3issue
10eissn
0021-9746issn
1472-4146journal_volume
34pub_type
杂志文章abstract::The assessment of emphysema in human lungs has traditionally been based on observations made on whole lung slices. These methods are inappropriate for the study of early emphysema, because as much as 75% of the alveolar wall surface area may have been lost by the time airspaces are visible to the naked eye. A new, aut...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.44.12.1007
更新日期:1991-12-01 00:00:00
abstract::A case of coexistent acute myeloid leukaemia and sarcoidosis is reported. This was complicated by recurrent pulmonary haemorrhage during reinduction and consolidation chemotherapy. A review of published papers on malignancy and sarcoidosis, in particular acute leukaemia is given. The outcome of most cases of acute leu...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1136/jcp.47.7.672
更新日期:1994-07-01 00:00:00
abstract:AIMS:Venous invasion (VI) is a known independent prognostic indicator of recurrence and survival in colorectal cancer. The guidelines of the Royal College of Pathologists (RCPath) state that, in a series of resections, extramural VI should be detected in at least 25% of specimens. However, there is widespread variabili...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2011-200156
更新日期:2011-11-01 00:00:00
abstract::Histological assessment of melanocytic naevi constitutes a substantial proportion of a dermatopathologist's daily workload. Although they may be excised for cosmetic reasons, most lesions encountered are clinically atypical and are biopsied or excised to exclude melanoma. Although dysplastic naevi are most often encou...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1136/jcp.2003.008516
更新日期:2004-11-01 00:00:00
abstract::The microwave tissue coagulator (MTC) is used in hepatectomy because it provides excellent haemostasis during the procedure. A 59 year old man underwent partial hepatic lobectomy with MTC, for metastasis from colon cancer. A tumorous necrotic nodule was discovered in the liver. The nodule measured 2.5 cm at its larges...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.50.8.703
更新日期:1997-08-01 00:00:00
abstract::A knowledge-based computer system, designed to assist pathologists in the histological diagnosis of breast disease, is described. This system represents knowledge in the form of "disease profiles" and uses a novel inference model based on the mathematical technique of hypergraphs. Its design overcomes many of the limi...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.44.6.502
更新日期:1991-06-01 00:00:00
abstract:AIMS:To investigate proliferative activity in leukaemic and lymphomatous bone marrow infiltrates and to assess the feasibility of transport of specimens among institutions. METHODS:Proliferative activity in bone marrow trephine cryosections from 99 patients with non-Hodgkin's lymphoma (NHL), 23 patients with acute mye...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.47.3.209
更新日期:1994-03-01 00:00:00
abstract:BACKGROUND:Clinical outcome in patients with diffuse large B cell lymphomas (DLBCL) is highly variable and poorly predictable. Microarray studies showed that patients with DLBCL with a germinal centre B cell-like (GCB) phenotype have a better prognosis than those with an activated B cell-like (ABC) phenotype. The BMI1 ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2006.038752
更新日期:2007-02-01 00:00:00
abstract::A pancreatic endocrine tumour arising within a serous cystadenoma is reported. A 49 year old woman was admitted with a history of epigastric pain, nausea, vomiting, and weight loss of two months duration. She had been diabetic for 12 years. An epigastric mass was palpated in the physical examination, and computed tomo...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.53.10.800
更新日期:2000-10-01 00:00:00
abstract:AIM:To evaluate the accuracy of the approximate linear formula of International Normalised Ratio (INR) imprecision by formal mathematical analysis. METHODS:Using probability theory, an exact formula for the coefficient of variation (CV) of the INR was derived. The CV from the approximate formula was compared with the ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.47.7.635
更新日期:1994-07-01 00:00:00
abstract::Liver diseases are associated with complex haemostasis defects, in which platelets, coagulation, and fibrinolysis may all be affected. The low plasma concentrations of clotting factors often found can be the result of many changes such as impaired synthesis, increased catabolism due to intravascular coagulation, or al...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.37.5.523
更新日期:1984-05-01 00:00:00
abstract::Of 30 children with bleeding disorders exposed to an index tuberculosis case, six developed primary pulmonary tuberculosis and two others became positive skin reactors. There was a significant correlation between the amount of replacement treatment received and the development of evidence of tuberculous infection. Onl...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.38.10.1163
更新日期:1985-10-01 00:00:00
abstract:AIMS:A series of patients with myeloma were investigated to assess whether immunological risk factors predisposing to serious infection could be identified. METHODS:Patients (n = 102) with predominantly plateau phase myeloma were monitored prospectively for infections. Immunological parameters including total non-para...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.48.3.260
更新日期:1995-03-01 00:00:00
abstract::A Cyto-Tek staining machine designed for Papanicolau staining of cervical smears has been adapted for auramine staining of smears of sputum and other clinical specimens for acid-fast bacilli. The results compared favourably with those obtained by a manual method of staining. Contamination of negative smears by acid-fa...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.31.2.185
更新日期:1978-02-01 00:00:00
abstract::The applicability of a hapten-antihapten sandwich technique incorporating a monoclonal antihapten bridge antibody to localisation of tissue antigen is described. The results show that the method is both versatile and selective and has the potential of being even more sensitive than currently used immunoperoxidase meth...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.34.9.1000
更新日期:1981-09-01 00:00:00
abstract::The development and evaluation of a new selective medium for Streptococcus pneumoniae is described. It is shown that the new medium (crystal violet-nalidixic acid-gentamicin agar:CVNG) is highly selective for Strep. pneumoniae in sputum specimens containing other organisms and is also sensitive, being only marginally ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.33.8.770
更新日期:1980-08-01 00:00:00
abstract:AIMS:To assess the clinical efficacy of diagnostic procedures for breast cancer at a teaching hospital using internal auditing tools and quality control measures. METHODS:A retrospective assessment of 500 patients who underwent core needle biopsy (wide-bore needle biopsy; WBN) of palpable or non-palpable breast nodes ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2011-200057
更新日期:2011-10-01 00:00:00
abstract::A polyclonal antiserum, produced in sheep and reactive against purified human leucocyte elastase, has been applied to paraffin sections from a range of human tissues by means of the indirect immunoperoxidase method. Striking and consistent results have been obtained. Normal or inflammatory granulocytes were intensely ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.37.10.1114
更新日期:1984-10-01 00:00:00
abstract:AIMS:To identify calcifying epithelial odontogenic tumour (CEOT) mutations in oncogenes and tumour suppressor genes. METHODS:A panel of 50 genes commonly mutated in cancer was sequenced in CEOT by next-generation sequencing. Sanger sequencing was used to cover the region of the frameshift deletion identified in one sa...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2017-204813
更新日期:2018-03-01 00:00:00
abstract:AIMS:The development of a technique for simultaneous in situ hybridisation for native mRNA and conventional immunofluorescence for cytoplasmic antigens in routine pathology specimens. METHODS:Cocktails of synthetic deoxyoligonucleotides coding for immunoglobulin J chain and kappa light chain were 3' end labelled enzym...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.45.2.114
更新日期:1992-02-01 00:00:00
abstract::Isolates of Campylobacter jejuni, C coli, C fetus and C laridis were tested for agglutination reactions with a panel of five lectins: Arachis hypogaea, Bauhinia purpurea, Solanum tuberosum, Triticum vulgaris and Wisteria floribunda. Twenty three patterns of agglutination (lectin types) were recorded among 376 isolates...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.43.11.957
更新日期:1990-11-01 00:00:00
abstract::One hundred and twenty-one rectal biopsies from 99 patients with carcinoma of the rectum or lower sigmoid colon were investigated using a high iron-diamine-Alcian blue technique for sulphated and non-sulphated acid mucins. It was found that in normal rectal mucosa sulphomucins are the main carbohydrate component of th...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.25.2.123
更新日期:1972-02-01 00:00:00
abstract::The results of the measurement of vitamin B(12) absorption by counting the radioactivity of 5 ml. serum obtained eight to 10 hours after the ingestion of an oral dose of 0.5 mug. vitamin B(12) labelled with 0.5 muc. (57)Co are compared with those obtained with the urinary excretion (Schilling) test. Inadequate urine c...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.19.6.606
更新日期:1966-11-01 00:00:00
abstract:AIM:Thalassaemia is a good candidate disease for control by preventive genetic programmes in developing countries. Accurate population frequency data are needed for planning the control of thalassaemia in the high risk Guangdong Province of southern China. METHODS:In total, 13397 consecutive samples from five geograph...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2003.014456
更新日期:2004-05-01 00:00:00
abstract:AIMS:To investigate the immunohistochemical expression of p53 protein in non-Hodgkin's lymphomas (NHL) and its relation to that of c-myc p62 oncoprotein and proliferating cell nuclear antigen (PCNA). METHODS:Paraffin wax embedded tissue from 90 non-Hodgkin's lymphomas (72 B cell and 18 T cell) was stained immunohistoc...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.47.1.9
更新日期:1994-01-01 00:00:00
abstract::A case is described in which marked hepatic fibrosis probably resulted from the prolonged treatment of acute leukaemia with methotrexate. ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.19.1.81
更新日期:1966-01-01 00:00:00
abstract::Neisseria gonorrhoeae is one of the most important causes of sexually transmitted disease. We do not fully understand the pathogenesis of infection with this organism, although recent improvements in immunological and molecular techniques have brought us closer to an answer. These techniques are now also being used to...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1136/jcp.40.9.1088
更新日期:1987-09-01 00:00:00
abstract::The air of three hospital wards was examined frequently for staphylococci for a period of nearly four years using slit samplers. ;Broadcasts' of staphylococci into the air were observed with the air count rising well above the mean of 0.2 staphylococcal particle per cubic foot and these broadcasts often appeared to be...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.15.6.552
更新日期:1962-11-01 00:00:00
abstract::The effect of temperature, time of storage, and presence of air bubbles in specimens for blood gas analysis was studied. The results show that air bubbles in a 10% proportion are undesirable because of significant elevation in the PO2, and the storage of anaerobic blood samples at room temperature (25 degrees C) is ac...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.33.9.864
更新日期:1980-09-01 00:00:00
abstract:BACKGROUND:A genetic component to the development of chronic fatigue syndrome (CFS) has been proposed, and a possible association between human leucocyte antigen (HLA) class II antigens and chronic fatigue immune dysfunction has been shown in some, but not all, studies. AIMS:To investigate the role of HLA class II ant...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2004.022681
更新日期:2005-08-01 00:00:00