A study of the interaction of lecithin: cholesterol acyltransferase with subfractions of high density lipoproteins.

abstract：:Membrane-protein interaction plays key roles in a wide variety of biological processes. Although various methods have been employed to measure membrane binding of soluble proteins, a robust high-throughput assay that is universally applicable to all proteins is lacking at present. Here we report a new fluorescence que...

journal_title：Journal of lipid research

authors： Kim H,Afsari HS,Cho W

更新日期：2013-12-01 00:00:00

abstract：:Serum paraoxonase (PON1) is a HDL-associated enzyme exhibiting potentially antiatherogenic properties. Here, we examined the common PON1-192R/Q human polymorphism. Despite numerous studies, the effect of this polymorphism on the antiatherogenic potential of PON1 is yet unresolved. Our structural model suggests that am...

journal_title：Journal of lipid research

authors： Gaidukov L,Rosenblat M,Aviram M,Tawfik DS

更新日期：2006-11-01 00:00:00

abstract：:Apolipoprotein A-I (apoA-I) induces the translocation of newly synthesized cholesterol as well as caveolin-1 to the cytosolic lipid-protein particle (CLPP) fraction in astrocytes before its appearance in high density lipoprotein generated in the medium (Ito, J., Y. Nagayasu, K. Kato, R. Sato, and S. Yokoyama. 2002. Ap...

journal_title：Journal of lipid research

authors： Ito J,Li H,Nagayasu Y,Kheirollah A,Yokoyama S

更新日期：2004-12-01 00:00:00

abstract：:Functions of the gut microbiome have a growing number of implications for host metabolic health, with diet being one of the most significant influences on microbiome composition. Compelling links between diet and the gut microbiome suggest key roles for various macronutrients, including lipids, yet how individual clas...

journal_title：Journal of lipid research

authors： Lee MT,Le HH,Johnson EL

更新日期：2020-07-09 00:00:00

abstract：:The effect of inhibiting lysosomal protein degradation on the activity of 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase was determined using a mouse mammary cell line (TS-85) which expresses a temperature-sensitive mutation in the ubiquitin degradative pathway. Incubating cells for 18 hr in medium containi...

journal_title：Journal of lipid research

authors： Tanaka RD,Li AC,Fogelman AM,Edwards PA

更新日期：1986-03-01 00:00:00

abstract：:ATP-citrate lyase (ACL) is a key lipogenic enzyme that converts citrate in the cytoplasm to acetyl-CoA, the initial precursor that yields malonyl-CoA for fatty acid biosynthesis. As cytosolic citrate is derived from the tricarboxylic acid cycle in the mitochondrion, ACL catalyzes a critical reaction linking cellular g...

journal_title：Journal of lipid research

authors： Wang Q,Li S,Jiang L,Zhou Y,Li Z,Shao M,Li W,Liu Y

更新日期：2010-09-01 00:00:00

abstract：:We previously reported that a eucaloric, low fat, liquid formula diet enriched in simple carbohydrate markedly increased the synthesis of fatty acids in lean volunteers. To examine the diet sensitivity of obese subjects, 7 obese and 12 lean volunteers were given two eucaloric low fat solid food diets enriched in simpl...

journal_title：Journal of lipid research

authors： Hudgins LC,Hellerstein MK,Seidman CE,Neese RA,Tremaroli JD,Hirsch J

更新日期：2000-04-01 00:00:00

abstract：:Dietary n-3 long-chain PUFAs (LC-PUFAs) are associated with improvement in the parameters of the metabolic syndrome (MetS). Glucokinase regulatory protein (GCKR) is a key protein regulating intracellular glucose disposal. Our aim was to investigate: i) the relationship between the GCKR rs1260326 (Pro446Leu) polymorphi...

journal_title：Journal of lipid research

authors： Rousseaux J,Duhamel A,Dumont J,Dallongeville J,Molnar D,Widhalm K,Manios Y,Sjöström M,Kafatos A,Breidenassel C,Gonzales-Gross M,Cuenca-Garcia M,Censi L,Ascensión M,De Henauw S,Moreno LA,Meirhaeghe A,Gottrand F

更新日期：2015-09-01 00:00:00

abstract：:The pathogenesis of aortic valve stenosis (AS) is characterized by the accumulation of LDL-derived cholesterol in the diseased valves. Since LDL particles also contain plant sterols, we investigated whether plant sterols accumulate in aortic valve lesions. Serum samples were collected from 82 patients with severe AS a...

journal_title：Journal of lipid research

authors： Helske S,Miettinen T,Gylling H,Mäyränpää M,Lommi J,Turto H,Werkkala K,Kupari M,Kovanen PT

更新日期：2008-07-01 00:00:00

abstract：:Remnant-like emulsions were prepared with lipid compositions similar to remnants derived from triacylglycerol-rich lipoproteins. When injected into the bloodstream of conscious mice the remnant-like emulsions labeled with cholesteryl[13C]oleate were metabolized in the liver and the appearance of 13CO2 in the breath wa...

journal_title：Journal of lipid research

authors： Martins IJ,Redgrave TG

更新日期：1998-03-01 00:00:00

abstract：:A new sample preparation method coupled to GC-MS analysis was developed and validated for quantification of sulfate esters of pregnenolone (PREG-S) and dehydroepiandrosterone (DHEA-S) in rat brain. Using a solid-phase extraction recycling protocol, the results show that little or no PREG-S and DHEA-S (<1 pmol/g) is pr...

journal_title：Journal of lipid research

authors： Liere P,Pianos A,Eychenne B,Cambourg A,Liu S,Griffiths W,Schumacher M,Sjövall J,Baulieu EE

更新日期：2004-12-01 00:00:00

abstract：:In order to study the metabolic responses of humans consuming a diet moderately high in cholesterol content, we carried out a long-term sterol balance study, up to 25 weeks in duration. Two subjects, one normocholesterolemic and one hypercholesterolemic, were given, in sequence, a very low cholesterol diet and then a ...

journal_title：Journal of lipid research

authors： Lin DS,Connor WE

更新日期：1980-11-01 00:00:00

abstract：:Genetic defects in SAR1B GTPase inhibit chylomicron (CM) trafficking to the Golgi and result in a huge intraenterocyte lipid accumulation with a failure to release CMs and liposoluble vitamins into the blood circulation. The central aim of this study is to test the hypothesis that SAR1B deletion (SAR1B-/- ) disturbs e...

journal_title：Journal of lipid research

authors： Sané A,Ahmarani L,Delvin E,Auclair N,Spahis S,Levy E

更新日期：2019-10-01 00:00:00

abstract：:The kinetics of lecithin exchange between native lipoproteins was characterized for individual molecular species of lecithins of rat mesenteric lymph chylomicrons and rat plasma HDL. Studies were performed in the absence of lipid transfer proteins. Donor (chylomicrons) and acceptor (HDL) particles were present in rati...

journal_title：Journal of lipid research

authors： Patton GM,Robins SJ,Fasulo JM,Clark SB

更新日期：1985-11-01 00:00:00

abstract：:Membranes of mammalian cells contain lysophosphatidic acid acyltransferase (LPAAT) activities that catalyze the acylation of sn-1-acyl lysophosphatidic acid (lysoPA) to form phosphatidic acid. As the biological roles and biochemical properties of the six known LPAAT isoforms have yet to be fully elucidated, we have ch...

journal_title：Journal of lipid research

authors： Hollenback D,Bonham L,Law L,Rossnagle E,Romero L,Carew H,Tompkins CK,Leung DW,Singer JW,White T

更新日期：2006-03-01 00:00:00

abstract：:The in vivo total body cholesterol transport of homozygous apoE-deficient (-/-) and control (+/+) mice was evaluated by compartmental analysis of plasma cholesterol decay. Body cholesterol fractional catabolic rates of chow fed mutants were less (-/-, 0.17 +/- 0.02; +/+, 0.51 +/- 0.06 day-1) and body cholesterol conte...

journal_title：Journal of lipid research

authors： Quarfordt SH,Oswald B,Landis B,Xu HS,Zhang SH,Maeda N

更新日期：1995-06-01 00:00:00

abstract：:The diffusion of [2-(14)C]glucose out of liposomes prepared from extracted human erythrocyte lipids was examined. Increased glucose efflux was observed when the lipids were treated with hydrogen peroxide and CuCl(2) before liposome formation, and this phenomenon required both peroxide and metal. Peroxidation of these ...

journal_title：Journal of lipid research

authors： Smolen JE,Shohet SB

更新日期：1974-05-01 00:00:00

abstract：:A novel and rapid method for the detergent-mediated synthesis of protein-lipid complexes has been developed and has several advantages over detergent dialysis methods. This new method involves co-incubation of human apolipoprotein A-I (apoA-I), the major protein component of high density lipoproteins (HDL), and dipalm...

journal_title：Journal of lipid research

authors： Bonomo EA,Swaney JB

更新日期：1988-03-01 00:00:00

abstract：:3 alpha,7 alpha-dihydroxy-5 beta-cholestan-26-oic acid (DHCA) and 3 alpha,7 alpha,12 alpha-trihydroxy-5 beta-cholestan-26-oic acid (THCA) are metabolized into chenodeoxycholic acid and cholic acid, respectively, through oxidation and cleavage of the terminal three carbons of the side chain. The present study was desig...

journal_title：Journal of lipid research

authors： Cass OW,Williams GC,Hanson RF

更新日期：1980-02-01 00:00:00

abstract：:Small angle X-ray diffraction was used to examine arterial smooth muscle cell (SMC) plasma membranes isolated from control and cholesterol-fed (2%) atherosclerotic rabbits. A microsomal membrane enriched with plasma membrane obtained from animals fed cholesterol for up to 13 weeks showed a progressive elevation in the...

journal_title：Journal of lipid research

authors： Tulenko TN,Chen M,Mason PE,Mason RP

更新日期：1998-05-01 00:00:00

abstract：:The Delta6-desaturase catalyzes key steps in long-chain polyunsaturated fatty acid biosynthesis. Although the gene coding for this enzyme has been isolated in diverse animal species, the protein structure remains poorly characterized. In this work, rat Delta6-desaturase expressed in COS-7 cells was shown to localize i...

journal_title：Journal of lipid research

authors： Guillou H,D'Andrea S,Rioux V,Barnouin R,Dalaine S,Pedrono F,Jan S,Legrand P

更新日期：2004-01-01 00:00:00

abstract：:HDL subspecies Lp(A-I) and Lp(A-I,A-II) have different anti-atherogenic potentials. To determine the role of lipoprotein lipase (LPL) and hepatic lipase (HL) in regulating these particles, we measured these enzyme activities in 28 healthy subjects with well-controlled Type 1 diabetes, and studied their relationship wi...

journal_title：Journal of lipid research

authors： Cheung MC,Sibley SD,Palmer JP,Oram JF,Brunzell JD

更新日期：2003-08-01 00:00:00

abstract：:In a previous study, it was shown that the peroxisomal fraction of rat liver, isolated by Percoll gradient centrifugation of a light mitochondrial fraction, was able to catalyze conversion of 3 alpha, 7 alpha, 12 alpha-trihydroxy-5 beta-cholestanoic acid (THCA) into cholic acid (Pedersen, J. I., and J. Gustafsson, 198...

journal_title：Journal of lipid research

authors： Kase F,Björkhem I,Pedersen JI

更新日期：1983-12-01 00:00:00

abstract：:Triacylglycerols (TG) are the major storage form of energy in eukaryotic organisms and are synthesized primarily by acyl CoA:1,2-diacylglycerol acyltransferase (DGAT) enzymes. In vitro DGAT activity has previously been quantified by measuring the incorporation of either radiolabeled fatty acyl CoA or diacylglycerol (D...

journal_title：Journal of lipid research

authors： McFie PJ,Stone SJ

更新日期：2011-09-01 00:00:00

abstract：:Dysbetalipoproteinemia, an uncommon but highly atherogenic mixed hyperlipidemia due to the accumulation of remnants of triglyceride-rich lipoproteins, is characterized by cholesterol-enriched VLDL that migrates in the beta-position on agarose gels. The demonstration of a broad beta-band on agarose gel electrophoresis ...

journal_title：Journal of lipid research

authors： Blom DJ,Byrnes P,Jones S,Marais AD

更新日期：2003-01-01 00:00:00

abstract：:Previous studies have reported both positive and negative effects of culture of islets at high glucose concentrations on regulated insulin secretion. Here, we have reexamined this question in mouse islets and determined the role of changes in lipid synthesis in the effects of glucose. Glucose-stimulated insulin secret...

journal_title：Journal of lipid research

authors： Diraison F,Ravier MA,Richards SK,Smith RM,Shimano H,Rutter GA

更新日期：2008-04-01 00:00:00

abstract：:Previous findings with various murine tumor cell lines suggest an association between ganglioside GM3 and cell cohesive properties. The influence of GM3 on cohesion was studied in two mouse brain tumor cell lines: ependymoblastoma (EPEN) and CT-2A. In culture, the EPEN cells grow as islands and contain GM3 as the only...

journal_title：Journal of lipid research

authors： Bai H,Seyfried TN

更新日期：1997-01-01 00:00:00

abstract：:This review presents an overview of mammalian phospholipid synthesis and the cellular locations of the biochemical activities that produce membrane lipid molecular species. The generalized endoplasmic reticulum compartment is a central site for membrane lipid biogenesis, and examples of the emerging relationships betw...

journal_title：Journal of lipid research

authors： Fagone P,Jackowski S

更新日期：2009-04-01 00:00:00

abstract：:Described herein are the chemical syntheses of a number of deuterated derivatives of 5 alpha-cholest-8(14)-en-3 beta-ol-15-one. These include the [2,2,3 alpha,4,4,7,7,9 alpha,16,16-2H10]-, [7 alpha,9 alpha,16,16-2H4]-, [7,7,9 alpha,16,16-2H5]-, and [2,2,3 alpha,4,4-2H5]-analogs of the delta 8(14)-15-ketosterol. Also i...

journal_title：Journal of lipid research

authors： St Pyrek J,Wilson WK,Schroepfer GJ Jr

更新日期：1987-11-01 00:00:00

abstract：:Lipid concentrations are heritable traits. Recently, the number of known genetic loci associated with lipid levels in adults increased from 95 to 157. The effects of these 157 loci have not been tested in children. Considering that lipid levels track from childhood to adulthood, we studied to determine whether these v...

journal_title：Journal of lipid research

authors： Latsuzbaia A,Jaddoe VW,Hofman A,Franco OH,Felix JF

更新日期：2016-12-01 00:00:00