Membrane phospholipid synthesis and endoplasmic reticulum function.

abstract：:Biosynthesis of 5,15-dihydroxyeicosatetraenoic acid (5,15-diHETE) in leukocytes involves consecutive oxygenation of arachidonic acid by 5-lipoxygenase (LOX) and 15-LOX in either order. Here, we analyzed the contribution of cyclooxygenase (COX)-2 to the biosynthesis of 5,15-diHETE and 5,11-diHETE in isolated human leuk...

journal_title：Journal of lipid research

authors： Tejera N,Boeglin WE,Suzuki T,Schneider C

更新日期：2012-01-01 00:00:00

abstract：:Rab GTPases require special machinery for protein prenylation, which include Rab escort protein (REP) and Rab geranylgeranyl transferase (RGGT). The current model of Rab geranylgeranylation proposes that REP binds Rab and presents it to RGGT. After geranylgeranylation of Rab C-terminal cysteines, REP delivers the pren...

journal_title：Journal of lipid research

authors： Leung KF,Baron R,Seabra MC

更新日期：2006-03-01 00:00:00

abstract：:N-3 trans geometrical isomers of 20:5 n-3 and 22:6 n-3 were isolated from rats fed heated linseed oil. The ability of these acids to inhibit 20:4 n-6 metabolism by human platelets was examined. The concentrations required to inhibit 50% of platelet aggregation (IC50) induced by 2.5 microM 20:4 n-6 were higher for the ...

journal_title：Journal of lipid research

authors： O'Keefe SF,Lagarde M,Grandgirard A,Sebedio JL

更新日期：1990-07-01 00:00:00

abstract：:Four distinct subspecies of the insect hemolymph lipoprotein, lipophorin, that range in diacylglycerol (DAG) content from approximately 100 to 1000 molecules per particle, were treated with phospholipase C. Lipid analysis demonstrated that both phosphatidylcholine and phosphatidylethanolamine were hydrolyzed to DAG. P...

journal_title：Journal of lipid research

authors： Singh TK,Liu H,Bradley R,Scraba DG,Ryan RO

更新日期：1994-09-01 00:00:00

abstract：:Phospholipids serve as central structural components in cellular membranes and as potent mediators in numerous signaling pathways. There are six main classes of naturally occurring phospholipids distinguished by their distinct polar head groups that contain many unique molecular species with distinct fatty acid compos...

journal_title：Journal of lipid research

authors： Baker PR,Armando AM,Campbell JL,Quehenberger O,Dennis EA

更新日期：2014-11-01 00:00:00

abstract：:In this study, we examined effects of retinol deficiency and three retinoids, all-trans-retinoic acid, 13-cis-retinoic acid, and etretin, the aromatic derivative of retinoic acid on nuclear retinoic acid receptor isoforms alpha, beta, and gamma mRNA in rat adipose tissue. Retinol deficiency caused an almost 50% decrea...

journal_title：Journal of lipid research

authors： Riaz-ul-Haq,Chytil F

更新日期：1992-03-01 00:00:00

abstract：:Most mammalian neoplasms have a defect in ether lipid content manifested by the presence of abnormally large quantities of 0-alkyl glyceryl ethers, in contrast to normal tissues in which the alk-1-enyl structure predominates. These lipids are for the most part structural. The manner in which tumor cell plasma membrane...

journal_title：Journal of lipid research

authors： Friedberg SJ,Halpert M

更新日期：1978-01-01 00:00:00

abstract：:Previous in vitro studies have revealed that oxidized low density lipoprotein (OxLDL) has negative effects on the proliferation and activity of endothelial progenitor cells (EPCs). Here, we evaluated the effect of OxLDL on the therapeutic potential of EPCs in ischemia-induced neovascularization. EPCs derived from mobi...

journal_title：Journal of lipid research

authors： Zhou B,Ma FX,Liu PX,Fang ZH,Wang SL,Han ZB,Poon MC,Han ZC

更新日期：2007-03-01 00:00:00

abstract：:The central region of apolipoprotein A-I (apoA-I), spanning residues 143--165, has been implicated in lecithin:cholesterol acyltransferase (LCAT) activation and also in high density lipoprotein (HDL) structural rearrangements. To examine the role of individual amino acids in these functions, we constructed, overexpres...

journal_title：Journal of lipid research

authors： Cho KH,Durbin DM,Jonas A

更新日期：2001-03-01 00:00:00

abstract：:apoE is a multi-functional protein expressed in several cell types and in several organs. It is highly expressed in adipose tissue, where it is important for modulating adipocyte lipid flux and gene expression in isolated adipocytes. In order to investigate a potential systemic role for apoE that is produced in adipos...

journal_title：Journal of lipid research

authors： Huang ZH,Reardon CA,Getz GS,Maeda N,Mazzone T

更新日期：2015-02-01 00:00:00

abstract：:The cellular mechanisms responsible for the lipoprotein-mediated stimulation of bile acid synthesis in cultured rat hepatocytes were investigated. Adding 280 micrograms/ml of cholesterol in the form of human or rat low density lipoprotein (LDL) to the culture medium increased bile acid synthesis by 1.8- and 1.6-fold, ...

journal_title：Journal of lipid research

authors： Junker LH,Davis RA

更新日期：1989-12-01 00:00:00

abstract：:In a previous study, it was shown that the peroxisomal fraction of rat liver, isolated by Percoll gradient centrifugation of a light mitochondrial fraction, was able to catalyze conversion of 3 alpha, 7 alpha, 12 alpha-trihydroxy-5 beta-cholestanoic acid (THCA) into cholic acid (Pedersen, J. I., and J. Gustafsson, 198...

journal_title：Journal of lipid research

authors： Kase F,Björkhem I,Pedersen JI

更新日期：1983-12-01 00:00:00

abstract：:Gluco- and galactocerebrosides can be separated by thin-layer chromatography on Silica Gel G prepared with sodium borate solution instead of water. The most successful developing system was chloroform-methanol-water-15 M NH(4)OH 280:70:6:1. ...

journal_title：Journal of lipid research

authors： Kean EL

更新日期：1966-05-01 00:00:00

abstract：:The primary structure of apolipoprotein A-II (apoA-II) isolated from the plasma high density lipoprotein (HDL) fraction of the inbred mouse strain BALB/c is described in this work. The complete 78 amino acid protein sequence was determined by proteolytic fragmentation, gas-phase microsequence analysis, and fast atom b...

journal_title：Journal of lipid research

authors： Miller CG,Lee TD,LeBoeuf RC,Shively JE

更新日期：1987-03-01 00:00:00

abstract：:Ceramides containing phytosphingosine as base and one of the fatty acids 16:0, 18:0, 20:0, 22:0, 23:0, and 24:0, were prepared by direct coupling in the presence of a mixed carbodiimide. The ceramides were analyzed as the 1,3,4-tri-O-trimethylsilyl ether derivatives by gas-liquid chromatography-mass spectrometry. Gas ...

journal_title：Journal of lipid research

authors： Hammarström S

更新日期：1970-05-01 00:00:00

abstract：:The pathogenesis of aortic valve stenosis (AS) is characterized by the accumulation of LDL-derived cholesterol in the diseased valves. Since LDL particles also contain plant sterols, we investigated whether plant sterols accumulate in aortic valve lesions. Serum samples were collected from 82 patients with severe AS a...

journal_title：Journal of lipid research

authors： Helske S,Miettinen T,Gylling H,Mäyränpää M,Lommi J,Turto H,Werkkala K,Kupari M,Kovanen PT

更新日期：2008-07-01 00:00:00

abstract：:A rapid isocratic method for determining the total phosphatidylcholine and disaturated phosphatidylcholine levels in lung surfactant preparations by high performance liquid chromatography (HPLC) is described. The analysis was performed on a 3.9 x 300 mm mu-Porasil column with detection by refractive index. The lipids ...

journal_title：Journal of lipid research

authors： Scarim J,Ghanbari H,Taylor V,Menon G

更新日期：1989-04-01 00:00:00

abstract：:Acyl-CoA thioesterase (Acot)2 localizes to the mitochondrial matrix and hydrolyses long-chain fatty acyl-CoA into free FA and CoASH. Acot2 is expressed in highly oxi-dative tissues and is poised to modulate mitochondrial FA oxidation (FAO), yet its biological role is unknown. Using a model of adenoviral Acot2 overexpr...

journal_title：Journal of lipid research

authors： Moffat C,Bhatia L,Nguyen T,Lynch P,Wang M,Wang D,Ilkayeva OR,Han X,Hirschey MD,Claypool SM,Seifert EL

更新日期：2014-12-01 00:00:00

abstract：:Membrane-bound proteins have been proposed to mediate the transport of long-chain FA (LCFA) transport through the plasma membrane (PM). These proposals are based largely on reports that PM transport of LCFAs can be blocked by a number of enzymes and purported inhibitors of LCFA transport. Here, using the ratiometric p...

journal_title：Journal of lipid research

authors： Jay AG,Simard JR,Huang N,Hamilton JA

更新日期：2020-05-01 00:00:00

abstract：:In a previously described method for determining the activity of cerebroside galactosidase, the enzyme preparation was incubated with an emulsion of cerebroside which had been labeled in the galactose moiety. The liberated galactose was separated from the emulsion by liquid-liquid partitioning, but the presence of det...

journal_title：Journal of lipid research

authors： Radin NS,Arora RC

更新日期：1971-03-01 00:00:00

abstract：:Protein farnesylation is a form of posttranslational modification that occurs in most, if not all, eukaryotic cells. Inhibitors of protein farnesyltransferase (PFTIs) have been developed as anticancer chemotherapeutic agents. Using the knowledge gained from the development of PFTIs for the treatment of cancer, researc...

journal_title：Journal of lipid research

authors： Eastman RT,Buckner FS,Yokoyama K,Gelb MH,Van Voorhis WC

更新日期：2006-02-01 00:00:00

abstract：:The extent and site(s) of inhibition of cholesterol absorption by plant sterols, sitosterol and fucosterol, were studied in rats. The intragastric administration of a single emulsified lipid meal containing 25 mg [3H]cholesterol and 25 mg of either sitosterol or fucosterol inhibited the lymphatic absorption of cholest...

journal_title：Journal of lipid research

authors： Ikeda I,Tanaka K,Sugano M,Vahouny GV,Gallo LL

更新日期：1988-12-01 00:00:00

abstract：:In addition to its role in the uptake of apolipoprotein B (apoB)-containing lipoproteins, apoE promotes hepatic very low density lipoprotein-triglyceride (VLDL-TG) production in animal models. However, it is not known if apoE increases the amount of TG per VLDL particle or the number of VLDL particles secreted. VLDL-a...

journal_title：Journal of lipid research

authors： Maugeais C,Tietge UJ,Tsukamoto K,Glick JM,Rader DJ

更新日期：2000-10-01 00:00:00

abstract：:Neu-Laxova syndrome (NLS) is a very rare autosomal recessive congenital disorder characterized by disturbed development of the central nervous system and the skin and caused by mutations in any of the three genes involved in de novo l-serine biosynthesis: PHGDH, PSAT1, and PSPH l-Serine is essential for the biosynthes...

journal_title：Journal of lipid research

authors： Takeichi T,Okuno Y,Kawamoto A,Inoue T,Nagamoto E,Murase C,Shimizu E,Tanaka K,Kageshita Y,Fukushima S,Kono M,Ishikawa J,Ihn H,Takahashi Y,Akiyama M

更新日期：2018-12-01 00:00:00

abstract：:Oxidized forms of cholesterol (oxysterols) are present in atherosclerotic lesions and may play an active role in lesion development. For example, 7-ketocholesterol (7KC) inhibits cholesterol efflux from macrophage foam cells induced by apolipoprotein A-I (apoA-I). Such oxysterols may promote foam cell formation in ath...

journal_title：Journal of lipid research

authors： Gelissen IC,Rye KA,Brown AJ,Dean RT,Jessup W

更新日期：1999-09-01 00:00:00

abstract：:To identify novel fatty acid diol synthases, putative candidate sequences from Penicillium species were analyzed, and hydroxy fatty acid production by crude Penicillium enzyme extracts was assessed. Penicillium chrysogenum was found to produce an unknown dihydroxy fatty acid, a candidate gene implicated in this produc...

journal_title：Journal of lipid research

authors： Shin KC,Seo MJ,Oh DK

更新日期：2016-02-01 00:00:00

abstract：:AY 9944 is a novel inhibitor of cholesterol biosynthesis which appears to act on the Delta 7-reductase enzyme, interfering with the conversion of 7-dehydrocholesterol to cholesterol. In all tissues studied, with the exception of the lens, there was a reduction in cholesterol and a marked accumulation of 7-dehydrochole...

journal_title：Journal of lipid research

authors： Horlick L

更新日期：1966-01-01 00:00:00

abstract：:We used [2-13C1]glycerol to characterize very low density lipoprotein (VLDL)-triglyceride kinetics and intrahepatic glycerol metabolism in normal men (n = 4) after alcohol (EtOH) ingestion. [2-13C1]glycerol was infused before and after the consumption of 48 g EtOH or a placebo. Three additional subjects also received ...

journal_title：Journal of lipid research

authors： Siler SQ,Neese RA,Parks EJ,Hellerstein MK

更新日期：1998-12-01 00:00:00

abstract：:The surface area, A, of contracting fatty acid monolayers was measured as a function of time, t, at constant surface pressure. In the initial temporal phase, ln A was linear with radical t. In a subsequent steady-state phase, ln A was linear with t. The initial desorption coefficient for sodium palmitate, K(i), and th...

journal_title：Journal of lipid research

authors： Patil GS,Matthews RH,Cornwell DG

更新日期：1973-01-01 00:00:00

abstract：:During intestinal absorption amino acids must traverse the lipid-rich epithelial cell membrane, possibly in a lipid-soluble form. In a search for such a form, we have determined the ability of lipid extracted from intestinal mucosa to bind valine. After incubation in a valine-containing medium this lipid (defined as t...

journal_title：Journal of lipid research

authors： Reiser S,Christiansen PA

更新日期：1968-09-01 00:00:00