Abstract:
:The influence of decapitation, ketamine and thiopental anesthesia on some properties of rat heart mitochondria was compared. Polarographic analysis were performed, oxygen consumption rates, respiratory control index, ADP : O ratio and oxidation of exogenous NADH were determined. Electronmicroscopic and gasometric examinations were also conducted. Mitochondrial fractions from hearts of decapitated rats oxidized at a generally faster rate and mitochondria from such hearts exhibited a significantly lower RCI value. These differences can be explained through the action of uncoupler(s) present in the decapitated group. A hypothesis linking decapitation with the uncoupling effect of fatty acids released intracellularly during catecholamine-stimulated lipolysis is discussed. No differences in the ADP : O ratio and electronmicroscopic details between the groups were found. Gasometric determinations in both anesthetized groups did not show any marked dysfunction of the respiratory system. It is concluded that decapitation leads to some changes in the functional integrity of rat heart mitochondria. Ketamine and thiopental anesthesia can be used with equal success for the isolation of mitochondria from the heart.
journal_name
Basic Res Cardioljournal_title
Basic research in cardiologyauthors
Dutkiewicz T,Chelstowski Kdoi
10.1007/BF01907952subject
Has Abstractpub_date
1981-03-01 00:00:00pages
136-43issue
2eissn
0300-8428issn
1435-1803journal_volume
76pub_type
杂志文章abstract::The mechanism of ergonovine-provoked coronary vasospasm is poorly understood. We tested the effect of ergonovine in perfused hearts from normal and cholesterol-fed (18 weeks, 2% cholesterol diet) rabbits in a constant-flow Langendorff perfusion. Aortic perfusion pressure was monitored to measure coronary vascular resi...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF02070165
更新日期:1983-07-01 00:00:00
abstract::A methodological approach based on Frank's pressure-volume (P-V) diagram and model calculations for analyzing the determinants of stroke volume (SV) under conditions of altered ventricular geometrical configuration is discussed with respect to the underlying geometrical models. The approach is generalized in the sense...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF02190551
更新日期:1991-03-01 00:00:00
abstract::Six dogs were subjected to an acute stenosis of the left anterior descending coronary artery. The mean aortic pressure was fixed with a windkessel and an occluder on four different levels. A significant decrease of coronary blood flow and poststenotic coronary pressure was closely related to the level of mean aortic ...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF01906456
更新日期:1976-05-01 00:00:00
abstract::We measured the amount of 1,2-diacylglycerol (DAG) and its fatty acid composition in hamster hearts of various ages because 1,2-DAG has been recognized to be one of the intracellular second messengers. The highest level in 1,2-DAG was detected in 30-day-old hamster hearts. The heart weight increased about 2.5 times be...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF01906969
更新日期:1990-03-01 00:00:00
abstract::The hormone-sensitive adenylate cyclase is a multi-component system embedded in the lipid bilayer of the plasma membrane and serves as a signal transduction system for various membrane receptors. The complete system consists of various receptor molecules, which sensitize the external ligands, the effector enzyme adeny...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF01907422
更新日期:1986-01-01 00:00:00
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journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/s00395-009-0047-x
更新日期:2010-01-01 00:00:00
abstract::Coronary artery disease (CAD), also known as ischemic heart disease (IHD), is the leading cause of mortality in the western world, with developing countries showing a similar trend. With the increased understanding of the role of the immune system and inflammation in coronary artery disease, it was shown that macropha...
journal_title:Basic research in cardiology
pub_type: 杂志文章,评审
doi:10.1007/s00395-016-0554-5
更新日期:2016-07-01 00:00:00
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journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF01907920
更新日期:1985-09-01 00:00:00
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journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:
更新日期:1985-01-01 00:00:00
abstract::This paper reviews the recent data concerning the force-frequency relations in experimental animals with heart failure. After a brief overview of the nature of these relations, which are induced by 2 opposite phenomena, the potentiation of force of contraction by heart rate and the restitution process, the paper revie...
journal_title:Basic research in cardiology
pub_type: 杂志文章,评审
doi:10.1007/s003950050212
更新日期:1998-01-01 00:00:00
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journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF01907742
更新日期:1979-05-01 00:00:00
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journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/s00395-012-0307-z
更新日期:2012-11-01 00:00:00
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journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF01906800
更新日期:1978-11-01 00:00:00
abstract::We have compared in open-chest dogs the evolution of adenine nucleotides and creatine phosphate to that of regional mechanical function during ischemia and reperfusion. Reperfusion restored normal value of creatine phosphate whereas adenine nucleotide level remained depressed. The percentage of systolic shortening, wh...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF01908316
更新日期:1982-11-01 00:00:00
abstract::A previous study indicated that 5 mg/kg aspirin can reverse the cardioprotective effects of thromboxane A2 synthetase inhibitors. We determined in the present study if this dose of aspirin can also reverse the protective effects of the thromboxane A2/PGH2 receptor antagonist SQ 30,741 in the same model of coronary occ...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF02190542
更新日期:1991-03-01 00:00:00
abstract::The behavior of Ca(2+)-activated K+ channels of large conductance (BKCa) in smooth muscle cells, which were obtained from atherosclerotic plaque material (SMCP) and from media segments (SMCM) of human coronary arteries, were compared using the patch-clamp technique. Voltage-clamp protocols in cell-attached patches rev...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF00788518
更新日期:1997-08-01 00:00:00
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journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/s003950050096
更新日期:1998-08-01 00:00:00
abstract::If everything happens strictly according to the natural laws, which meet extremum principles, in what way can the possibility for life be characterised, so that the optimization processes of evolution can take place? Is it legitimate to "enlarge" the natural laws by certain laws of conservation? The question then aris...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:
更新日期:1993-01-01 00:00:00
abstract::Rate or frequency-dependence is a characteristic property of antiarrhythmic drugs belonging to the Vaughan William classes I and III. The rate-dependence of class I drugs (i.e., increasing blockade of fast Na(+)-channels with faster rates) results from periodical drug binding to Na(+)-channel sites which are preferabl...
journal_title:Basic research in cardiology
pub_type: 杂志文章,评审
doi:10.1007/s003950050236
更新日期:1998-01-01 00:00:00
abstract::Hypertension and resulting left ventricular hypertrophy was induced in young male Wistar rats (60 to 70 days old) by narrowing of one renal artery (Goldblatt II). 8 and 24 weeks after operation, myocardial oxygen consumption was measured on a modified in situ heart-lung preparation with nearly isovolumetric left ventr...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF01908041
更新日期:1982-05-01 00:00:00
abstract::Complete length-tension diagrams of myocardium were measured on isolated papillary muscles of cat right ventricle. These diagrams clearly demonstrate that irrespective of loading conditions there is no uniform maxima curve. As a function of preload the curves of afterloaded isotonic maxima obey totally separate nonlin...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF01907862
更新日期:1985-11-01 00:00:00
abstract::Myocardial ischemia and infarction are the most common substrates for life-threatening ventricular tachyarrhythmias. Experimental and clinical evidence suggests that enhanced activity of the sympathetic nervous system plays an important role in the genesis of ischemia-related arrhythmias. In animal experiments, beta-b...
journal_title:Basic research in cardiology
pub_type: 杂志文章,评审
doi:10.1007/978-3-662-11038-6_24
更新日期:1990-01-01 00:00:00
abstract::A model of vascular endothelial cell is proposed to describe the mechanisms by which cytosolic calcium (Cai) is modulated and endothelium-derived relaxing factor (EDRF) and prostacyclin (PGI2) are released when the cell is stimulated by agonist. The intracellular Ca2+ store of the model cell is comprised of a superfic...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF00796518
更新日期:1992-07-01 00:00:00
abstract::Cardiac hypertrophy which occurs during chronic mechanical overload is one of the numerous examples of biological adaptation to environmental requirements. As such, it is obtained at random by trial and error, and adaptation represents the sum of various modifications in gene expression, including the shift in isoform...
journal_title:Basic research in cardiology
pub_type: 杂志文章,评审
doi:10.1007/978-3-642-72474-9_15
更新日期:1992-01-01 00:00:00
abstract::Verapamil, at a dose of 1 mg/kg, was given intravenously to anesthetized cats one hour after coronary artery occlusion. Verapamil significantly reduced mean arterial blood pressure, but produced an increase in heart rate, partially offsetting the reduction in myocardial oxygen demand resulting from the reduction in pr...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/BF01907648
更新日期:1979-09-01 00:00:00
abstract::Recent reports indicate that elevating DNA glycosylase/AP lyase repair enzyme activity offers marked cytoprotection in cultured cells and a variety of injury models. In this study, we measured the effect of EndoIII, a fusion protein construct that traffics Endonuclease III, a DNA glycosylase/AP lyase, to the mitochond...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/s00395-014-0459-0
更新日期:2015-03-01 00:00:00
abstract::We compared the effects of linagliptin (Lina, a DPP4 inhibitor) and GLP-1 receptor activation by exenatide followed by exendin-4 in an infusion pump (EX) on infarct size (IS), post-infarction activation of the inflammasome and remodeling in wild-type (WT) and db/db diabetic mice. Mice underwent 30 min ischemia followe...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/s00395-019-0743-0
更新日期:2019-08-06 00:00:00
abstract::Cardiomyopathy is one of the most common causes of chronic heart failure worldwide. Mutations in the gene encoding nexilin (NEXN) occur in patients with both hypertrophic and dilated cardiomyopathy (DCM); however, little is known about the pathophysiological mechanisms and relevance of NEXN to these disorders. Here, w...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/s00395-015-0522-5
更新日期:2016-01-01 00:00:00
abstract::Reversibly injured myocardium after short periods of ischemia is characterized by a prolonged depression of contractile function which can, however, be enhanced by inotropic interventions. Thus, a lack of inotropic stimulation due to ischemic damage of cardiac sympathetic nerves has been suggested as a mechanism under...
journal_title:Basic research in cardiology
pub_type: 杂志文章
doi:10.1007/978-3-662-11038-6_22
更新日期:1990-01-01 00:00:00
abstract::Since the discovery of a novel mechanism of cell death that differs from traditional necrosis, i.e., apoptosis, there have been numerous studies concluding that increased apoptosis augments myocardial infarction and heart failure and that limiting apoptosis protects the heart. Importantly, the vast majority of cells i...
journal_title:Basic research in cardiology
pub_type: 杂志文章,评审
doi:10.1007/s00395-016-0549-2
更新日期:2016-05-01 00:00:00