Kernicterus: enzymatic evidence for difference between the development of cholinergic and GABAergic innervations in the brain of the Gunn rat.

abstract：:Peptidyl-glycine alpha-amidation enzyme activity has been measured in 36 nuclei or areas in the rat CNS and pituitary using D-Tyr-Phe-Gly as the substrate. The distribution of this enzyme is highly uneven, with highest activity levels (greater than 30 pmol/mg of protein/h) in hypothalamic nuclei, substantia grisea cen...

journal_title：Journal of neurochemistry

authors： Meng F,Tsou K

更新日期：1988-05-01 00:00:00

abstract：:Although pharmacological stimulation of a wide variety of transmitter receptors triggers phosphoinositide (PI) turnover, little is known about the type of synaptic activity required to activate this system. To investigate this question, we have used primary cultures of embryonic cortical neurons, which develop functio...

journal_title：Journal of neurochemistry

authors： Murphy TH,Wright DD,Baraban JM

更新日期：1992-12-01 00:00:00

abstract：:GABAA receptors are activated via low-affinity binding sites for the agonists GABA or muscimol. Evidence has been provided that the amino acid residue alpha 1F64 located at the beta2(+)/alpha1(-) subunit interface forms part of this binding site. In radioactive ligand binding studies the agonist [3H]muscimol has been ...

journal_title：Journal of neurochemistry

authors： Baur R,Sigel E

更新日期：2003-10-01 00:00:00

abstract：:A simple radioenzymatic method for the determination of DOPA is described. The method is based on the conversion of DOPA to 3-O-[methyl-3H]DOPA by catechol-O-methyltransferase in the presence of S-adenosyl-[methyl-3H]methionine and purification of the labelled product by Sephadex G10 and Dowex 50 W x 4 ion exchange re...

journal_title：Journal of neurochemistry

authors： Argiolas A,Gessa GL

更新日期：1981-01-01 00:00:00

abstract：:Glutamate is the predominant excitatory neurotransmitter in the mammalian central nervous system and extracellular glutamate levels are controlled by a family of transporters known as excitatory amino acid transporters (EAATs). The EAATs transport glutamate and aspartate with similar micromolar affinities and this tra...

journal_title：Journal of neurochemistry

authors： Ryan RM,Kortt NC,Sirivanta T,Vandenberg RJ

更新日期：2010-07-01 00:00:00

abstract：:The sodium-coupled, hemicholinium-3-sensitive, high-affinity choline transporter (CHT) is responsible for transport of choline into cholinergic nerve terminals from the synaptic cleft following acetylcholine release and hydrolysis. In this study, we address regulation of CHT function by plasma membrane cholesterol. We...

journal_title：Journal of neurochemistry

authors： Cuddy LK,Winick-Ng W,Rylett RJ

更新日期：2014-03-01 00:00:00

abstract：:The glutamate receptor 2 (GluR2) subunit determines many of the functional properties of the alpha-amino-3-hydroxy-5-methylisoxazole-4-propionate subtype of glutamate receptor. The roles of untranslated regions (UTRs) in mRNA stability, transport, or translation are increasingly recognized. The 3' end of the GluR2 tra...

journal_title：Journal of neurochemistry

authors： Irier HA,Shaw R,Lau A,Feng Y,Dingledine R

更新日期：2009-04-01 00:00:00

abstract：:Existence of microtubule cytoskeleton at the membrane and submembranous regions, referred as 'membrane tubulin' has remained controversial for a long time. Since we reported physical and functional interaction of Transient Receptor Potential Vanilloid Sub Type 1 (TRPV1) with microtubules and linked the importance of T...

journal_title：Journal of neurochemistry

authors： Goswami C

更新日期：2012-10-01 00:00:00

abstract：:To investigate the role of the retinoblastoma protein pRB in neuronal differentiation, we have measured the accumulation of hypophosphorylated pRB in PC12 cells stimulated by nerve growth factor (NGF). NGF induced the accumulation of hypophosphorylated pRB within 30 min and the level peaked after 12 h. Viral Kiras, cy...

journal_title：Journal of neurochemistry

authors： Li H,Kawasaki H,Nishida E,Hattori S,Nakamura S

更新日期：1996-06-01 00:00:00

abstract：:Mood, emotion, cognition, and motor functions as well as circadian and neuroendocrine rhythms, including food intake, sleep, and reproductive activity, are modulated by the midbrain raphe serotonin (5-HT) system. By directing the magnitude and duration of postsynaptic responses, carrier-facilitated 5-HT transport into...

journal_title：Journal of neurochemistry

authors： Heils A,Teufel A,Petri S,Stöber G,Riederer P,Bengel D,Lesch KP

更新日期：1996-06-01 00:00:00

abstract：:The effects of phenytoin, a potent antiepileptic drug, on the active transport of cations within membranes remain controversial. To assess the direct effects of phenytoin on the Na+,K+ pump, we studied the drug's influence on the phosphorylation of partially purified (Na+,K+)-ATPase from mouse brain. (Na+,K+)-ATPase s...

journal_title：Journal of neurochemistry

authors： Guillaume D,Grisar T,Delgado-Escueta AV

更新日期：1986-09-01 00:00:00

abstract：:Microtubule-associated proteins (MAPs) were phosphorylated by a Ca2+- and calmodulin-dependent protein kinase from rat brain cytosol. The maximal amount of phosphate incorporated into MAPs was 25 nmol of phosphate/mg protein. A Ka value of the enzyme for calmodulin was 57.0 nM, with MAPs as substrates. Among MAPs, MAP...

journal_title：Journal of neurochemistry

authors： Yamamoto H,Fukunaga K,Tanaka E,Miyamoto E

更新日期：1983-10-01 00:00:00

abstract：:To provide insights into the effects of temporary focal ischemia on the function of neurons and astrocytes in vivo, we measured the incorporation of radiolabel from [U-14C]glucose into both glutamate and glutamine in brain subregions at 1 h of reperfusion following occlusion of the middle cerebral artery for 2 or 3 h....

journal_title：Journal of neurochemistry

authors： Thoren AE,Helps SC,Nilsson M,Sims NR

更新日期：2006-05-01 00:00:00

abstract：:Rarefaction of the dendritic tree leading to neuronal dysfunction is a hallmark of many neurodegenerative diseases and we have shown previously that heat shock protein B5 (HspB5)/αB-crystallin is able to increase dendritic complexity in vitro. The aim of this study was to investigate if this effect is also present in ...

journal_title：Journal of neurochemistry

authors： Bartelt-Kirbach B,Wiegreffe C,Birk S,Baur T,Moron M,Britsch S,Golenhofen N

更新日期：2020-11-21 00:00:00

abstract：:Hypoxic preconditioning (HP) 24 h before hypoxic-ischemic (HI) injury confers significant neuroprotection in neonatal rat brain. Recent studies have shown that the mitogen-activated protein kinase (MAPK) and phosphatidylinositol-3-kinase (PI3K) intracellular signaling pathways play a role in the induction of tolerance...

journal_title：Journal of neurochemistry

authors： Jones NM,Bergeron M

更新日期：2004-04-01 00:00:00

abstract：:We examined the potential importance of dexamethasone-mediated alterations in energy metabolism in providing protection against hypoxic-ischemic brain damage in immature rats. Seven-day-old rats (n = 165) that had been treated with dexamethasone (0.1 mg/kg, i.p.) or vehicle were assigned to control or hypoxic-ischemic...

journal_title：Journal of neurochemistry

authors： Tuor UI,Yager JY,Bascaramurty S,Del Bigio MR

更新日期：1997-11-01 00:00:00

abstract：:The brain adapts to dynamic environmental conditions by altering its epigenetic state, thereby influencing neuronal transcriptional programs. An example of an epigenetic modification is protein methylation, catalyzed by protein arginine methyltransferases (PRMT). One member, Prmt8, is selectively expressed in the cent...

journal_title：Journal of neurochemistry

authors： Lee PK,Goh WW,Sng JC

更新日期：2017-02-01 00:00:00

abstract：:In contrast to brain, the sciatic nerve concentration of vitamin E in rats increased rapidly during the postnatal period (approximately fivefold between days 1 and 8), then decreased dramatically (about twofold between days 8 and 30), and further decreased slowly between days 30 and 60 and remained constant up to 2 ye...

journal_title：Journal of neurochemistry

authors： Clément M,Bourre JM

更新日期：1990-06-01 00:00:00

abstract：:We examined the axonal transport of actin and its monomer binding proteins, actin depolymerizing factor, cofilin, and profilin, in the chicken sciatic nerve following injection of [35S]methionine into the lumbar spinal cord. At intervals up to 20 days after injection, nerves were cut into 1-cm segments and separated i...

journal_title：Journal of neurochemistry

authors： Mills RG,Minamide LS,Yuan A,Bamburg JR,Bray JJ

更新日期：1996-09-01 00:00:00

abstract：:The ability of activated glia to affect cerebral vascular tone has been evaluated using an in vitro experimental system in which basilar arteries were incubated with glial cultures activated by treatment with lipopolysaccharide (LPS). Vascular tone was measured with an isometric myograph. Contraction in response to hi...

journal_title：Journal of neurochemistry

authors： Chisari M,Salomone S,Laureanti F,Copani A,Sortino MA

更新日期：2004-12-01 00:00:00

abstract：:Primary astrocyte cultures from neonatal rat brains show uptake of [3H]norepinephrine ([3H]NE). This uptake has a high-affinity component with an apparent Km of approximately 3 X 10(-7) M. At 10(-7) M [3H]NE both the initial rate of uptake and steady-state content of [3H]NE is inhibited by up to 95% by omission of ext...

journal_title：Journal of neurochemistry

authors： Kimelberg HK,Pelton EW 2nd

更新日期：1983-05-01 00:00:00

abstract：:The regulation of glial and neuronal Na+-dependent glutamate/aspartate transporters is of interest because abnormal glutamate transport may be responsible for certain neurological diseases. Because expression of the Wnt-1 protooncogene results in induction of the glial-type glutamate transporter GLAST in PC12 neuron-l...

journal_title：Journal of neurochemistry

authors： Palos TP,Zheng S,Howard BD

更新日期：1999-09-01 00:00:00

abstract：:Sequence variation was found in cDNA coding for the extracellular domain of the rat gamma-aminobutyric acid type A (GABAA) receptor alpha 6 subunit. About 20% of polymerase chain reaction (PCR)-amplified alpha 6 cDNA prepared from rat cerebellar mRNA lacked nucleotides 226-255 as estimated by counting single-stranded ...

journal_title：Journal of neurochemistry

authors： Korpi ER,Kuner T,Kristo P,Köhler M,Herb A,Lüddens H,Seeburg PH

更新日期：1994-09-01 00:00:00

abstract：:Mechanical transection of the nigrostriatal dopamine pathway at the medial forebrain bundle (MFB) results in the delayed degeneration of dopaminergic neurons in the substantia nigra pars compacta (SNpc). We have previously demonstrated that c-Jun activation is an obligate component of neuronal death in this model. Her...

journal_title：Journal of neurochemistry

authors： Crocker SJ,Hayley SP,Smith PD,Mount MP,Lamba WR,Callaghan SM,Slack RS,Park DS

更新日期：2006-01-01 00:00:00

abstract：:Although palmitoylation of the beta(2)-adrenergic receptor (beta(2)AR), as well as its phosphorylation by the cyclic AMP-dependant protein kinase (PKA) and the beta-adrenergic receptor kinase (beta ARK), are known to play important roles in agonist-promoted desensitization, their relative contribution and mutual regul...

journal_title：Journal of neurochemistry

authors： Moffett S,Rousseau G,Lagacé M,Bouvier M

更新日期：2001-01-01 00:00:00

abstract：:The aim of this study is to examine how sustained exposure to two 1,4-benzodiazepines (BZDs) with different action period, diazepam and brotizolam, and a 1,5-BZD, clobazam, affects L-type high voltage-gated calcium channel (HVCC) functions and its mechanisms using primary cultures of mouse cerebral cortical neurons. T...

journal_title：Journal of neurochemistry

authors： Katsura M,Shibasaki M,Kurokawa K,Tsujimura A,Ohkuma S

更新日期：2007-12-01 00:00:00

abstract：:Neuroleptics, which are potent dopamine receptor antagonists, are used to treat psychosis. In the striatum, dopamine subtype-2 (D2) receptors interact with high-affinity adenosine subtype-2 (A2a) receptors. To examine the effect of various neuroleptics on the major subtypes of striatal dopamine and adenosine receptors...

journal_title：Journal of neurochemistry

authors： Parsons B,Togasaki DM,Kassir S,Przedborski S

更新日期：1995-11-01 00:00:00

abstract：:The effects of treatment with the anti-convulsant agents, lamotrigine and riluzole were compared with gabapentin in a rat experimental model of neuropathic pain. Rats were treated intraperitoneally, with gabapentin (30, 100 and 300 mg/kg), lamotrigine (2, 10 and 50 mg/kg) or riluzole (6 and 12 mg/kg) prior to, and eve...

journal_title：Journal of neurochemistry

authors： Coderre TJ,Kumar N,Lefebvre CD,Yu JS

更新日期：2007-03-01 00:00:00

abstract：:Cultures of neonatal rat superior cervical ganglia (SCG) were used to test the hypothesis that the cytokines leukemia inhibitory factor (LIF) and ciliary neurotrophic factor (CNTF) control GTP cyclohydrolase I (GTPCH) gene expression and 5,6,7,8-tetrahydrobiopterin (BH4) content as traits of the noradrenergic phenotyp...

journal_title：Journal of neurochemistry

authors： Stegenga SL,Hirayama K,Kapatos G

更新日期：1996-06-01 00:00:00

abstract：:Phosphoinositide hydrolysis was studied in neurohybrid NCB-20 cells prelabeled with myo-[3H]inositol. Among nearly 20 neurotransmitters and neuromodulators examined, only bradykinin, carbachol, and histamine significantly increased the accumulation of [3H]inositol monophosphate (IP1) in the presence of lithium. The EC...

journal_title：Journal of neurochemistry

authors： Chuang DM,Dillon-Carter O

更新日期：1988-08-01 00:00:00