Abstract：
:Although pharmacological stimulation of a wide variety of transmitter receptors triggers phosphoinositide (PI) turnover, little is known about the type of synaptic activity required to activate this system. To investigate this question, we have used primary cultures of embryonic cortical neurons, which develop functional glutamate and GABA synapses during maturation in vitro. Mature cultures display spontaneous synaptic activity that is totally suppressed by tetrodotoxin (TTX). PI turnover, assayed by the lithium-sensitive accumulation of [3H]CDP-diacylglycerol, was readily detected under basal conditions and was abolished by TTX. Increased excitatory synaptic activity induced by picrotoxin, an antagonist of GABAA receptor-mediated inhibition, further stimulated PI turnover. Similar results were obtained when PI turnover was assayed using [3H]inositol labeling. With either assay, the magnitude of synaptically induced PI turnover was comparable to maximal responses produced by muscarinic receptor stimulation. Although a component of the spontaneous synaptic currents is sensitive to N-methyl-D-aspartate (NMDA)-preferring glutamate receptor antagonists, blockade of NMDA receptors did not affect PI turnover associated with synaptic transmission. To assess the time course of synaptically mediated PI turnover, the amplitude and duration of spontaneous synaptic currents were reduced by lowering the extracellular Ca2+ concentration from 2.25 to 0.5 mM, a maneuver that suppresses basal PI turnover. Increases in PI turnover were detected as early as 5 min following restoration of the extracellular Ca2+ concentration to 2.25 mM. Taken together, these findings indicate that activation of the PI system is associated with physiological levels of glutamatergic synaptic transmission.
journal_name
J Neurochemjournal_title
Journal of neurochemistryauthors
Murphy TH,Wright DD,Baraban JMdoi
10.1111/j.1471-4159.1992.tb10130.xkeywords:
subject
Has Abstractpub_date
1992-12-01 00:00:00pages
2336-9issue
6eissn
0022-3042issn
1471-4159journal_volume
59pub_type
杂志文章abstract：:Patients with cancer cachexia often suffer from psychiatric disorders. In the present study, we investigated the changes in monoaminergic activities in the brain in tumor-bearing mice with reference to the development of cachexia. Two clones, clone-5 (noncachectic clone) and clone-20 (cachectic clone), derived from th...
journal_title：Journal of neurochemistry
pub_type： 杂志文章
doi：10.1046/j.1471-4159.1998.70010260.x
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abstract：:We studied the time course of oxidatively modified proteins in the nigrostriatal dopaminergic system following transection of the medial forebrain bundle by quantifying the number of carbonyl groups coupled to striatal and nigral protein homogenates, an index of metal-catalyzed oxidations. We found a striking effect o...
journal_title：Journal of neurochemistry
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doi：10.1046/j.1471-4159.1997.68062458.x
更新日期：1997-06-01 00:00:00
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journal_title：Journal of neurochemistry
pub_type： 杂志文章
doi：10.1111/j.1471-4159.1990.tb04162.x
更新日期：1990-08-01 00:00:00
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pub_type： 杂志文章
doi：10.1111/j.1471-4159.1990.tb04970.x
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journal_title：Journal of neurochemistry
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doi：10.1111/j.1471-4159.1981.tb01610.x
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更新日期：2009-01-01 00:00:00
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更新日期：2000-05-01 00:00:00
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journal_title：Journal of neurochemistry
pub_type： 杂志文章
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journal_title：Journal of neurochemistry
pub_type： 杂志文章
doi：10.1111/j.1471-4159.1985.tb07203.x
更新日期：1985-11-01 00:00:00
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更新日期：2012-02-01 00:00:00
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journal_title：Journal of neurochemistry
pub_type： 杂志文章
doi：10.1111/j.1471-4159.1983.tb12658.x
更新日期：1983-01-01 00:00:00
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journal_title：Journal of neurochemistry
pub_type： 杂志文章
doi：10.1111/j.1471-4159.1990.tb04899.x
更新日期：1990-06-01 00:00:00
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journal_title：Journal of neurochemistry
pub_type： 杂志文章
doi：10.1046/j.1471-4159.1996.66010169.x
更新日期：1996-01-01 00:00:00
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doi：10.1046/j.1471-4159.1994.62031126.x
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journal_title：Journal of neurochemistry
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journal_title：Journal of neurochemistry
pub_type： 杂志文章
doi：10.1111/j.1471-4159.1990.tb13288.x
更新日期：1990-01-01 00:00:00
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更新日期：1997-08-01 00:00:00
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更新日期：2006-01-01 00:00:00
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journal_title：Journal of neurochemistry
pub_type： 杂志文章
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更新日期：2017-02-01 00:00:00
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journal_title：Journal of neurochemistry
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doi：10.1111/j.1471-4159.1980.tb09022.x
更新日期：1980-12-01 00:00:00
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更新日期：2004-11-01 00:00:00
