Effect of pertussis toxin on radioligand binding to rat brain adenosine A1 receptors.

abstract：:Activation of extracellular signal-regulated protein kinase 1/2 (ERK1/2) are implicated in the pathophysiology of spinal cord injury (SCI). However, the specific functions of individual ERK isoforms in neurodegeneration are largely unknown. We investigated the hypothesis that ERK2 activation may contribute to patholog...

journal_title：Journal of neurochemistry

authors： Yu CG,Yezierski RP,Joshi A,Raza K,Li Y,Geddes JW

更新日期：2010-04-01 00:00:00

abstract：:Proteolytic fragmentation of [3H]diisopropylfluorophosphate-labelled catalytic subunits of different molecular forms of acetylcholinesterase demonstrates that all forms extracted from the electric organ from Torpedo marmorata are true acetylcholinesterases. This is supported by immunochemical results showing that the ...

journal_title：Journal of neurochemistry

authors： Stieger S,Brodbeck U,Witzemann V

更新日期：1987-08-01 00:00:00

abstract：:The critical hyaluronan binding motif (HABM) in sialoprotein associated with cones and rods (SPACR) has already been determined. As sialoproteoglycan associated with cones and rods, another interphotoreceptor matrix molecule, binds to chondroitin sulfate and heparin with or without the employment of HABMs, respectivel...

journal_title：Journal of neurochemistry

authors： Zhao J,Yoneda M,Takeyama M,Inoue Y,Kataoka T,Ohno-Jinno A,Isogai Z,Iwaki M,Zako M

更新日期：2008-11-01 00:00:00

abstract：:The effect of clinical, spontaneous-onset seizures on extracellular fluid lactate was investigated by the method of lactography, the in vivo on-line measurement of lactate levels using microdialysis. Studies of experimental animals have suggested that generation of extracellular lactate as measured by microdialysis is...

journal_title：Journal of neurochemistry

authors： During MJ,Fried I,Leone P,Katz A,Spencer DD

更新日期：1994-06-01 00:00:00

abstract：:The function of membrane phospholipids (PL) in the regulation of gamma-aminobutyric acid (GABA) transport and GABA carrier binding has been investigated in organized cultures of rat cerebral cortex. The cellular lipid composition has been changed by growing the cells in a delipidated nutrient solution or by short-term...

journal_title：Journal of neurochemistry

authors： Giesing M,Gerken U,Kastrup H

更新日期：1985-03-01 00:00:00

abstract：:Ganglioside GM1, tritiated at the level of the long chain base (sphingosine) [( Sph-3H]GM1), sialic acid (N-acetylneuraminic acid) [( NeuAc-3H]GM1), or terminal galactose [( Gal-3H]GM1) was supplied to cerebellar granule cells differentiated in vitro, and its metabolic processing was followed with pulse time. Using [S...

journal_title：Journal of neurochemistry

authors： Riboni L,Tettamanti G

更新日期：1991-12-01 00:00:00

abstract：:Reduced levels of the protein frataxin cause the neurodegenerative disease Friedreich's ataxia. Pathology is associated with disruption of iron-sulfur cluster biosynthesis, mitochondrial iron overload, and oxidative stress. Frataxin is a highly conserved iron-binding protein present in most organisms. Despite the inte...

journal_title：Journal of neurochemistry

authors： Pastore A,Puccio H

更新日期：2013-08-01 00:00:00

abstract：:beta-Methyleneaspartate, a specific inhibitor of aspartate aminotransferase (EC 2.6.1.1.), was used to investigate the role of the malate-aspartate shuttle in rat brain synaptosomes. Incubation of rat brain cytosol, "free" mitochondria, synaptosol, and synaptic mitochondria, with 2 mM beta-methyleneaspartate resulted ...

journal_title：Journal of neurochemistry

authors： Cheeseman AJ,Clark JB

更新日期：1988-05-01 00:00:00

abstract：:Levels of dopamine (DA), 3,4-dihydroxyphenylacetic acid (DOPAC), homovanillic acid (HVA), noradrenaline (NA), 3-methoxy-4-hydroxyphenylglycol (MHPG), and 5-hydroxyindoleacetic acid (5-HIAA) in the CSF of patients with Huntington's disease (HD) were measured by HPLC. CSF DA, DOPAC, and MHPG levels were found to be incr...

journal_title：Journal of neurochemistry

authors： Garrett MC,Soares-da-Silva P

更新日期：1992-01-01 00:00:00

abstract：:High-affinity binding sites (apparent KD = 1.5 nM) for [3H]desipramine have been demonstrated and characterized in membranes prepared from rat brain. The binding of [3H]desipramine was found to be saturable, reversible, heat-sensitive, sodium-dependent, and regionally distributed among various regions of the brain. Hi...

journal_title：Journal of neurochemistry

authors： Rehavi M,Skolnick P,Brownstein MJ,Paul SM

更新日期：1982-04-01 00:00:00

abstract：:Differential assembly of N-methyl-D-aspartate (NMDA) receptor subunits determines their functional characteristics. Using in situ hybridization, we found a selective increase of the subunits NR1 and NR2A mRNA at 24 h in ventral motor neurons (VMN) caudal to a standardized spinal cord contusion injury (SCI). Other neur...

journal_title：Journal of neurochemistry

authors： Grossman SD,Wolfe BB,Yasuda RP,Wrathall JR

更新日期：2000-07-01 00:00:00

abstract：:Deposit of beta-amyloid protein (A beta) in Alzheimer's disease brain may contribute to the associated neurodegeneration. We have studied the neurotoxicity of A beta in primary cultures of murine cortical neurons, with the aim of identifying pharmacologic ways of attenuating the injury. Exposure of cultures to A beta ...

journal_title：Journal of neurochemistry

authors： Weiss JH,Pike CJ,Cotman CW

更新日期：1994-01-01 00:00:00

abstract：:The anti-diabetic drug and peroxisome proliferator-activated receptor-gamma (PPARγ) agonist, rosiglitazone, alters astrocyte activation; however, its mechanism remains less-known. We hypothesized participation of epidermal growth factor receptor (EGFR), known to control astrocyte reactivity. We first detected that ros...

journal_title：Journal of neurochemistry

authors： Kushwaha R,Mishra J,Gupta AP,Gupta K,Vishwakarma J,Chattopadhyay N,Gayen JR,Kamthan M,Bandyopadhyay S

更新日期：2019-06-01 00:00:00

abstract：:Major membrane lipids were quantified in frontal (Brodmann area 9) and temporal (Brodmann areas 21 and 22) cortices, caudate nucleus, hippocampus, and frontal white matter of 12 cases with Alzheimer's disease (AD) type I (early onset), 21 cases with AD type II (late onset), and 20 age-matched controls. The concentrati...

journal_title：Journal of neurochemistry

authors： Svennerholm L,Gottfries CG

更新日期：1994-03-01 00:00:00

abstract：:Microtubule-associated protein (MAP) 1B is a high-molecular-weight cytoskeletal protein that is abundant in developing neuronal processes and appears to be necessary for axonal growth. Various biochemical and immunocytochemical results are reported, indicating that a significant fraction of MAP1B is expressed as an in...

journal_title：Journal of neurochemistry

authors： Tanner SL,Franzen R,Jaffe H,Quarles RH

更新日期：2000-08-01 00:00:00

abstract：:Clearance rates for serotonin (5-HT) in heterozygote (+/-) and homozygote (-/-) serotonin transporter (5-HTT) knockout (KO) mice have not been determined in vivo. Moreover, the effect of selective serotonin reuptake inhibitors (SSRIs) on 5-HT clearance in these mice has not been examined. In this study, the rate of cl...

journal_title：Journal of neurochemistry

authors： Montañez S,Owens WA,Gould GG,Murphy DL,Daws LC

更新日期：2003-07-01 00:00:00

abstract：:Electrophorus electroplax microsomes were examined for Ca2+- and Mg2+-dependent ATPase activity. In addition to the previously reported low-affinity ATPase, a high-affinity (Ca2+,Mg2+)-ATPase was found. At low ATP and Mg2+ concentrations (200 microM or less), the high-affinity (Ca2+,Mg2+)-ATPase exhibits an activity o...

journal_title：Journal of neurochemistry

authors： Amende LM,Chock SP,Albers RW

更新日期：1983-04-01 00:00:00

abstract：:The distribution of cerebellar gangliosides was studied in staggerer (sg/sg) mutant mice, where the majority of granule cells die after completing their migration across the molecular layer. In addition, the external granule cell layer in sg/sg mice persists longer than in normal mice. Moreover, in the sg/sg cerebellu...

journal_title：Journal of neurochemistry

authors： Seyfried TN,Bernard DJ,Yu RK

更新日期：1984-10-01 00:00:00

abstract：:The structure and function of mitochondrial respiratory-chain enzyme proteins were studied postmortem in the substantia nigra of nine patients with Parkinson's disease and nine matched controls. Total protein and mitochondrial mass were similar in the two groups. NADH-ubiquinone reductase (Complex I) and NADH cytochro...

journal_title：Journal of neurochemistry

authors： Schapira AH,Cooper JM,Dexter D,Clark JB,Jenner P,Marsden CD

更新日期：1990-03-01 00:00:00

abstract：:PEA-15 has recently been identified as a major phosphoprotein in astrocytes and an endogenous substrate for protein kinase C. This 15-kDa protein exists under three molecular forms, an unphosphorylated form, N, and two phosphorylated forms, Pa and Pb. Antisera were raised against synthetic peptides corresponding to th...

journal_title：Journal of neurochemistry

authors： Danziger N,Yokoyama M,Jay T,Cordier J,Glowinski J,Chneiweiss H

更新日期：1995-03-01 00:00:00

abstract：:The effect of 6-aminonicotinamide (6AN) treatment on the activities of alternative pathways of glucose metabolism in 20-day-old rat brain was evaluated by measurements of yields of 14CO2 from glucose labeled with 14C on carbons 1, 2, 3 + 4, or 6 and uniformly labeled glucose, and from the incorporation of 14C from spe...

journal_title：Journal of neurochemistry

authors： Hothersall JS,Zubairu S,McLean P,Greenbaum AL

更新日期：1981-12-01 00:00:00

abstract：:The microtubule-based motor protein kinesin is thought to drive anterograde organelle transport in axons, but nothing is known about how its force-generating activity or organelle-binding properties are regulated. Studies in other motility systems suggest that protein phosphorylation is a reasonable candidate for this...

journal_title：Journal of neurochemistry

authors： Hollenbeck PJ

更新日期：1993-06-01 00:00:00

abstract：:Single-bilayer vesicles of myelin are desirable for studying myelin development and metabolism. Accordingly, our interest was drawn to a procedure for vesiculating myelin (Steck et al., Biochim, Biophys. Acta 509, 397-408, 1978). We used X-ray diffraction analysis to examine these putative vesicle preparations because...

journal_title：Journal of neurochemistry

authors： Sedzik J,Toews AD,Blaurock AE,Morell P

更新日期：1984-11-01 00:00:00

abstract：:A reporter epitope was inserted at 11 positions in a region encompassing proposed transmembrane segments S1 and S2 in the second repeat domain (D2) of the rat skeletal muscle type 1 sodium channel. All mutations produced full-length membrane-associated protein following transfection into cultured cells, although the l...

journal_title：Journal of neurochemistry

authors： Kraner SD,Filatov GN,Sun W,Bannerman P,Lindstrom J,Barchi RL

更新日期：1998-04-01 00:00:00

abstract：:To study the role of intracellular pH (pHi) in catecholamine secretion and the regulation of pHi in bovine chromaffin cells, the pH-sensitive fluorescent indicator [2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein] was used to monitor the on-line changes in pHi. The pHi of chromaffin cells at resting state is approxima...

journal_title：Journal of neurochemistry

authors： Kao LS,Ho MY,Cragoe EJ Jr

更新日期：1991-11-01 00:00:00

abstract：:In the present study, the properties, such as pH optima, detergent requirement, and effects of various lipids, of cholesterol ester hydrolase in human cerebrospinal fluid (CSF) were examined, and the activity levels of the enzyme in CSF from multiple sclerosis (MS) patients and non-MS individuals were compared. Our da...

journal_title：Journal of neurochemistry

authors： Johnson RC,Shah SN

更新日期：1981-09-01 00:00:00

abstract：:A neutral, mannose-rich, concanavalin A (Con A)-binding glycopeptide fraction was obtained by proteolytic digestion of defatted beef brain tissue. Hydrazinolysis followed by gel filtration of the reaction products provided three oligosaccharides. A portion of each oligosaccharide was treated by exhaustive digestion wi...

journal_title：Journal of neurochemistry

authors： Somawardhana CW,Brunngraber EG

更新日期：1983-08-01 00:00:00

abstract：:The inhibitors of apoptosis (IAPs) are emerging as key proteins in the control of cell death. In this study, we evaluated the expression and subcellular distribution of the antiapoptotic protein X-linked IAP (XIAP), and its interactions with the XIAP-associated factor 1 (XAF1) in neonatal rat brain following hypoxia-i...

journal_title：Journal of neurochemistry

authors： Russell JC,Whiting H,Szuflita N,Hossain MA

更新日期：2008-08-01 00:00:00

abstract：:Diminished energy metabolism and reduced activity of brain alpha-ketoglutarate dehydrogenase complex (KGDHC) occur in a number of neurodegenerative diseases. The relation between diminished KGDHC activity and altered energy metabolism is unknown. The present study tested whether a reduction in the KGDHC activity would...

journal_title：Journal of neurochemistry

authors： Shi Q,Risa Ø,Sonnewald U,Gibson GE

更新日期：2009-05-01 00:00:00

abstract：:This study examines the proteolysis of the carboxy terminal domain of the full-length (alpha 1(212)) and truncated (alpha 1(190)) forms of the rabbit skeletal muscle L-type calcium channel alpha 1 subunit by calpain I and calpain II. Although both forms of the alpha 1 subunit show little sensitivity to proteolysis by ...

journal_title：Journal of neurochemistry

authors： De Jongh KS,Colvin AA,Wang KK,Catterall WA

更新日期：1994-10-01 00:00:00