Exaggerated effect of fluvoxamine in heterozygote serotonin transporter knockout mice.


:Clearance rates for serotonin (5-HT) in heterozygote (+/-) and homozygote (-/-) serotonin transporter (5-HTT) knockout (KO) mice have not been determined in vivo. Moreover, the effect of selective serotonin reuptake inhibitors (SSRIs) on 5-HT clearance in these mice has not been examined. In this study, the rate of clearance of exogenously applied 5-HT was measured in the CA3 region of the hippocampus of anesthetized mice using high-speed chronoamperometry. Compared with wild-type mice, the maximal rate of 5-HT clearance from extracellular fluid (ECF) was decreased in heterozygotes and more markedly so in KO mice. Heterozygote mice were more sensitive to the 5-HT uptake inhibitor, fluvoxamine, resulting in longer clearance times for 5-HT than in wild-type mice; as expected, the KO mice were completely unresponsive to fluvoxamine. There were no associated changes in norepinephrine transporter density, nor was there an effect of the norepinephrine uptake inhibitor, desipramine, on 5-HT clearance in any genotype. Thus, adaptive changes in the norepinephrine transport system do not occur in the CA3 region of hippocampus as a consequence of 5-HTT KO. These data highlight the potential of the heterozygote 5-HTT mutant mice to model the dynamic in vivo consequences of the human 5-HTT polymorphism.


J Neurochem


Montañez S,Owens WA,Gould GG,Murphy DL,Daws LC





Has Abstract


2003-07-01 00:00:00














  • Uptake of gamma-aminobutyric acid by a synaptic vesicle fraction isolated from rat brain.

    abstract::Gamma-Aminobutyric acid (GABA) was taken up by a MgATP-dependent mechanism into synaptic vesicles isolated by hypoosmotic shock and density gradient centrifugation. The properties of the vesicular uptake differed clearly from those of synaptosomal and glial uptake, both with respect to Na+, Mg2+, and ATP dependence an...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Fykse EM,Fonnum F

    更新日期:1988-04-01 00:00:00

  • Molecular dissection of native mammalian forebrain NMDA receptors containing the NR1 C2 exon: direct demonstration of NMDA receptors comprising NR1, NR2A, and NR2B subunits within the same complex.

    abstract::The subunit compositions of the NR1 C2 exon-containing N-methyl-D-aspartate (NMDA) receptors of adult mammalian forebrain were determined by using a combination of immunoaffinity chromatography and immunoprecipitation studies with NMDA receptor subunit-specific antibodies. NMDA receptors were solubilised by sodium deo...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Chazot PL,Stephenson FA

    更新日期:1997-11-01 00:00:00

  • Alteration of alpha-tocopherol content in the developing and aging peripheral nervous system: persistence of high correlations with total and specific (n-6) polyunsaturated fatty acids.

    abstract::In contrast to brain, the sciatic nerve concentration of vitamin E in rats increased rapidly during the postnatal period (approximately fivefold between days 1 and 8), then decreased dramatically (about twofold between days 8 and 30), and further decreased slowly between days 30 and 60 and remained constant up to 2 ye...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Clément M,Bourre JM

    更新日期:1990-06-01 00:00:00

  • An HSV-1 vector expressing tyrosine hydroxylase causes production and release of L-dopa from cultured rat striatal cells.

    abstract::In this report we demonstrate that a defective herpes simplex virus type one (HSV-1) vector can express enzymatically active tyrosine hydroxylase in cultured striatal cells that are thereby converted into L-DOPA-producing cells. A human tyrosine hydroxylase cDNA (form II) was inserted into an HSV-1 vector (pHSVth) and...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Geller AI,During MJ,Oh YJ,Freese A,O'Malley K

    更新日期:1995-02-01 00:00:00

  • Interleukin-1beta: a bridge between inflammation and excitotoxicity?

    abstract::Interleukin-1 (IL-1) is a proinflammatory cytokine released by many cell types that acts in both an autocrine and/or paracrine fashion. While IL-1 is best described as an important mediator of the peripheral immune response during infection and inflammation, increasing evidence implicates IL-1 signaling in the pathoge...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章,评审


    authors: Fogal B,Hewett SJ

    更新日期:2008-07-01 00:00:00

  • Effect of insulin-induced hypoglycemia on the concentrations of glutamate and related amino acids and energy metabolites in the intact and decorticated rat neostriatum.

    abstract::The glutamate (Glu) terminals in rat neostriatum were removed by a unilateral frontal decortication. One to two weeks later the effects of insulin-induced hypoglycemia on the steady-state levels of amino acids [Glu, glutamine (Gln), aspartate (Asp), gamma-aminobutyric acid (GABA), taurine] and energy metabolites (gluc...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Engelsen B,Westerberg E,Fonnum F,Wieloch T

    更新日期:1986-11-01 00:00:00

  • Modulation of cannabinoid agonist binding by 5-HT in the rat cerebellum.

    abstract::Cross-talk between cannabinoid CB1 and serotonin 5-HT receptors in rat cerebellar membranes was investigated using radioligand binding. In competition against the CB1 antagonist, [3 H]SR141716A, the agonist, WIN 55,212-2 yielded a biphasic isotherm. The majority of binding was to a high-affinity state that was signifi...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Devlin MG,Christopoulos A

    更新日期:2002-03-01 00:00:00

  • Nerve growth factor stimulates the production of inositol 1,3,4- and 1,4,5-trisphosphate and inositol 1,3,4,5-tetrakisphosphate in PC12 cells.

    abstract::In PC12 cells, preincubated with [3H]inositol, nerve growth factor (NGF) stimulated an approximately 100% increase in the levels of [3H]inositol 1,3,4-trisphosphate ([3H]-Ins(1,3,4)P3], [3H]inositol 1,4,5-trisphosphate ([3H]Ins(1,4,5)P3], and [3H]inositol 1,3,4,5-tetrakisphosphate ([3H]Ins(1,3,4,5)P4] as early as 5-15...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Contreras ML

    更新日期:1993-09-01 00:00:00

  • Characterization of two chromaffin cell populations isolated from bovine adrenal medulla.

    abstract::Cultures of chromaffin cells isolated from the bovine adrenal medulla have been extremely useful for investigating secretory mechanisms, but such cultures used up to the present time represent mixed populations of adrenergic and noradrenergic cells. This report describes how, with slight modifications to standard proc...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Moro MA,Garcia AG,Langley OK

    更新日期:1991-08-01 00:00:00

  • Purinergic modulation of hippocampal acetylcholine release involves alpha-dendrotoxin-sensitive potassium channels.

    abstract::Modulation of acetylcholine (ACh) release from superfused hippocampal slices was examined when the release of ACh was stimulated by exposure of slices to elevated K+ concentration. Evoked release was not sensitive to inhibition by 0.1 microM tetrodotoxin, but it could be inhibited in a dose-dependent manner by a musca...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Benishin CG

    更新日期:1990-12-01 00:00:00

  • Tyrosine phosphorylation of the alpha subunit of transducin and its association with Src in photoreceptor rod outer segments.

    abstract::Recent evidence indicates that tyrosine phosphorylation may play important roles in retinal photoreceptor rod outer segments (ROS). We investigated the tyrosine phosphorylation of endogenous proteins in isolated bovine ROS. Several proteins with apparent molecular masses of 31, 39, 60, 83, 90, 97, 120, 140, and 180 kD...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Bell MW,Desai N,Guo XX,Ghalayini AJ

    更新日期:2000-11-01 00:00:00

  • Competitive inhibition of N-acetylated-alpha-linked acidic dipeptidase activity by N-acetyl-L-aspartyl-beta-linked L-glutamate.

    abstract::The endogenous neuropeptide N-acetyl-L-aspartyl-L-glutamate (NAAG) fulfills several criteria required to be accepted as a neurotransmitter. NAAG inactivation may proceed through enzymatic hydrolysis into N-acetyl-L-aspartate and glutamate by an N-acetylated-alpha-linked acidic dipeptidase (NAALADase). Therefore, some ...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Serval V,Barbeito L,Pittaluga A,Cheramy A,Lavielle S,Glowinski J

    更新日期:1990-07-01 00:00:00

  • Synergistic induction of pituitary adenylate cyclase-activating polypeptide (PACAP) gene expression by nerve growth factor and PACAP in PC12 cells.

    abstract::Pituitary adenylate cyclase-activating polypeptide (PACAP) gene expression was analyzed in PC12 cells. PC12 cells transfected with a PACAP promoter-luciferase reporter construct were utilized to investigate the effects of PACAP, either alone or in combination with nerve growth factor (NGF), on PACAP transcriptional re...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Hashimoto H,Hagihara N,Koga K,Yamamoto K,Shintani N,Tomimoto S,Mori W,Koyama Y,Matsuda T,Baba A

    更新日期:2000-02-01 00:00:00

  • GTP cyclohydrolase I gene expression in the brains of male and female hph-1 mice.

    abstract::The hph-1 mouse is characterized by low levels of GTP cyclohydrolase I (GTPCH) and tetrahydrobiopterin. A quantitative double-label in situ hybridization technique was used to examine CNS GTPCH mRNA expression within serotonin, dopamine, and norepinephrine neurons of male and female wild-type and hph-1 mice. In wild-t...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Shimoji M,Hirayama K,Hyland K,Kapatos G

    更新日期:1999-02-01 00:00:00

  • Melatonin biosynthesis in Drosophila: its nature and its effects.

    abstract::Drosophila melanogaster homogenates incubated with tritiated 5-hydroxytryptophan, 5-hydroxytryptamine, or N-acetylserotonin have the ability of converting them into labelled indolamines, including melatonin. All these compounds were characterized by two-dimensional thin-layer chromatography by comparison with nonradio...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Finocchiaro L,Callebert J,Launay JM,Jallon JM

    更新日期:1988-02-01 00:00:00

  • Metabotropic glutamate receptor subtype mGluR1alpha stimulates the secretion of the amyloid beta-protein precursor ectodomain.

    abstract::To examine the effects of glutamatergic neurotransmission on amyloid processing, we stably expressed the metabotropic glutamate receptor subtype 1alpha (mGlu-R1alpha) in HEK 293 cells. Both glutamate and the selective metabotropic agonist 1-amino-1,3-cyclopentanedicarboxylic acid (ACPD) rapidly increased phosphatidyli...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Nitsch RM,Deng A,Wurtman RJ,Growdon JH

    更新日期:1997-08-01 00:00:00

  • Characterization of [3H]quipazine binding to 5-hydroxytryptamine3 receptors in rat brain membranes.

    abstract::[3H]Quipazine was used to label binding sites in rat brain membranes that display characteristics of a 5-hydroxytryptamine3 (5-HT3) receptor. The radioligand binds with high affinity (KD, 1.2 +/- 0.1 nM) to a saturable population of sites (Bmax, 3.0 +/- 0.4 pmol/g of tissue) that are differentially located in the brai...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Milburn CM,Peroutka SJ

    更新日期:1989-06-01 00:00:00

  • Cytoplasmic poly(ADP-ribose) polymerase associated with free messenger ribonucleoprotein particles in rat brain.

    abstract::Poly(ADP-ribose) polymerase associated with free cytoplasmic messenger ribonucleoprotein particles (free mRNP particles) carrying messenger RNA has been characterized in rat brain. There were first-order kinetics for NAD with an apparent Km for NAD of 90.5 +/- 0.70 microM and Vmax of 19.7 +/- 2.8 pmol ADP-ribose incor...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Chypre C,Maniez C,Mandel P

    更新日期:1988-08-01 00:00:00

  • Selective destruction of cultured dopaminergic neurons from fetal rat mesencephalon by 1-methyl-4-phenylpyridinium: cytochemical and morphological evidence.

    abstract::Dopaminergic neurons in cultures of dissociated cells from fetal rat mesencephalon were exposed to the principal metabolite of the neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), 1-methyl-4-phenyl-pyridinium ion (MPP+), and several of its structural analogues. At concentrations between 0.01 and 0.1 mic...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Sanchez-Ramos JR,Michel P,Weiner WJ,Hefti F

    更新日期:1988-06-01 00:00:00

  • Inhibition of glucosylceramide synthase stimulates autophagy flux in neurons.

    abstract::Aggregate-prone mutant proteins, such as α-synuclein and huntingtin, play a prominent role in the pathogenesis of various neurodegenerative disorders; thus, it has been hypothesized that reducing the aggregate-prone proteins may be a beneficial therapeutic strategy for these neurodegenerative disorders. Here, we ident...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Shen W,Henry AG,Paumier KL,Li L,Mou K,Dunlop J,Berger Z,Hirst WD

    更新日期:2014-06-01 00:00:00

  • Understanding depression: the hippocampus might hold the answer in a CREB-regulated transcription coactivator: An Editorial for 'AAV-mediated over-expression of CRTC1 in the hippocampal dentate gyrus ameliorates lipopolysaccharide-induced depression-like

    abstract::The pathophysiology of major depressive disorders is not completely understood. In this issue of Journal of Neurochemistry, Ni and colleagues investigate the role of cyclic adenosine monophosphate response element-binding protein (CREB)-dependent signaling in the hippocampus on depressive-like behaviors. This editoria...

    journal_title:Journal of neurochemistry

    pub_type: 评论,社论


    authors: Davis RL

    更新日期:2019-04-01 00:00:00

  • Haptoglobin increases the vulnerability of CD163-expressing neurons to hemoglobin.

    abstract::Haptoglobin (Hp) binds hemoglobin (Hb) with high affinity and provides the primary defense against its toxicity after intravascular hemolysis. Neurons are exposed to extracellular Hb after CNS hemorrhage, and a therapeutic effect of Hp via Hb sequestration has been hypothesized. In this study, we tested the hypothesis...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Chen-Roetling J,Regan RF

    更新日期:2016-11-01 00:00:00

  • Characterization of quinolinic acid phosphoribosyltransferase in human blood and observations in Huntington's disease.

    abstract::Quinolinic acid (QUIN), an excitotoxic compound present in the mammalian CNS and periphery, has been hypothetically linked to human neurodegenerative disorders such as Huntington's disease and epilepsy. Quinolinic acid phosphoribosyltransferase (QPRT), the catabolic enzyme of QUIN, is found in the CNS and peripheral o...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Foster AC,Schwarcz R

    更新日期:1985-07-01 00:00:00

  • Metabolic products of [2-(13) C]ethanol in the rat brain after chronic ethanol exposure.

    abstract::Most ingested ethanol is metabolized in the liver to acetaldehyde and then to acetate, which can be oxidized by the brain. This project assessed whether chronic exposure to alcohol can increase cerebral oxidation of acetate. Through metabolism, acetate may contribute to long-term adaptation to drinking. Two groups of ...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Wang J,Du H,Ma X,Pittman B,Castracane L,Li TK,Behar KL,Mason GF

    更新日期:2013-11-01 00:00:00

  • Labelling of "peripheral-type" benzodiazepine binding sites in the rat brain by using [3H]PK 11195, an isoquinoline carboxamide derivative: kinetic studies and autoradiographic localization.

    abstract::PK 11195 [1-(2-chlorophenyl)-N-methyl-N-(1-methylpropyl)-3-isoquinolinecarboxamide] is a new ligand for the "peripheral-type" benzodiazepine binding sites, chemically unrelated to benzodiazepines. It displaces with a very high potency (IC50 congruent to 10(-9) M) [3H]-RO5-4864 (a benzodiazepine which specifically labe...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Benavides J,Quarteronet D,Imbault F,Malgouris C,Uzan A,Renault C,Dubroeucq MC,Gueremy C,Le Fur G

    更新日期:1983-12-01 00:00:00

  • Regulation of transmitter gamma-aminobutyric acid (GABA) synthesis and metabolism illustrated by the effect of gamma-vinyl GABA and hypoglycemia.

    abstract::The effect of different treatments on amino acid levels in neostriatum was studied to throw some light on the synthesis and metabolism of gamma-aminobutyric acid (GABA). Irreversible inhibition of GABA transaminase by microinjection of gamma-vinyl GABA (GVG) led to a decrease in aspartate, glutamate, and glutamine lev...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Paulsen RE,Fonnum F

    更新日期:1988-04-01 00:00:00

  • Phorbol ester-induced upregulation of angiotensin II receptors in neuronal cultures is potentiated by a calcium ionophore.

    abstract::Previous studies have suggested that protein kinase C is important in the regulation of angiotensin II receptors in neuronal cultures, because the C-kinase agonists, phorbol esters, are able to increase the number of these receptors. In the present study, we have further investigated the role of protein kinase C in an...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Sumners C,Rueth SM,Myers LM,Kalberg CJ,Crews FT,Raizada MK

    更新日期:1988-07-01 00:00:00

  • Multiple molecular forms of acetylcholinesterase in rat vagus nerve, smooth muscle, and heart.

    abstract::Extracts of rat tissues were subjected to ultracentrifugation on linear density gradients of sucrose (5-20%) and fractions of these gradients were analyzed for acetylcholinesterase (AChE) activity. Most of the AChE activity in extracts of vagus nerve was found to sediment at 10S. However, 16S AChE, previously thought ...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Skau KA,Brimijoin S

    更新日期:1980-11-01 00:00:00

  • Estrogen and tamoxifen reverse manganese-induced glutamate transporter impairment in astrocytes.

    abstract::Chronic exposure to manganese (Mn) can cause manganism, a neurodegenerative disorder similar to Parkinson's disease. The toxicity of Mn includes impairment of astrocytic glutamate transporters. 17beta-Estradiol (E2) has been shown to be neuroprotective in various neurodegenerative diseases including Parkinson's diseas...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Lee ES,Sidoryk M,Jiang H,Yin Z,Aschner M

    更新日期:2009-07-01 00:00:00

  • Lipid metabolism during early stages of Wallerian degeneration in the rat sciatic nerve.

    abstract::We examined changes in biosynthetic capacity of sciatic nerve during the early stages of Wallerian degeneration, utilizing a model that permits exclusion of nonresident cells from degenerating nerve. Sciatic nerve segments were placed in either 5-microns pore (allowing infiltration of nonresident cells) or 0.22-micron...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: White FV,Toews AD,Goodrum JF,Novicki DL,Bouldin TW,Morell P

    更新日期:1989-04-01 00:00:00