Evidence for two cholesterol ester hydrolases in human cerebrospinal fluid.

abstract：:The normal cellular form of prion protein (PrP(C)) has been shown to exhibit a diverse range of biological activities. Several recent studies highlighted potential involvement of PrP(C) in embryogenesis or in regulating stem cell self-renewal and proliferation. In the current study, we employed human embryonic stem ce...

journal_title：Journal of neurochemistry

authors： Lee YJ,Baskakov IV

更新日期：2010-07-01 00:00:00

abstract：:The ontogeny of chick brain and heart ventricle calcium antagonist binding sites was determined, using [3H]nitrendipine ([3H]NDP), as the ligand. The binding of [3H]NDP to adult heart and brain was kinetically very similar, with the former displaying a KD of 0.28 +/- 0.02 nM and a Bmax of 138 +/- 17 fmol/mg protein, a...

journal_title：Journal of neurochemistry

authors： Marangos PJ,Sperelakis N,Patel J

更新日期：1984-05-01 00:00:00

abstract：:Immunoaffinity chromatography with a monoclonal antibody produced against bovine tau protein was used to purify tau proteins from human brain. Fifty grams of brain tissue yielded approximately 2 mg of pure tau proteins. The affinity-purified human tau was used to produce a high-titered rabbit anti-human tau serum. The...

journal_title：Journal of neurochemistry

authors： Mercken M,Vandermeeren M,Lübke U,Six J,Boons J,Vanmechelen E,Van de Voorde A,Gheuens J

更新日期：1992-02-01 00:00:00

abstract：:Microfluorimetric measurements of intracellular calcium ion concentration [Ca(2+)](i) were employed to examine the effects of chronic hypoxia (2.5% O(2), 24 h) on Ca(2+) stores and capacitative Ca(2+) entry in human neuroblastoma (SH-SY5Y) cells. Activation of muscarinic receptors evoked rises in [Ca(2+)](i) which wer...

journal_title：Journal of neurochemistry

authors： Smith IF,Boyle JP,Vaughan PF,Pearson HA,Peers C

更新日期：2001-11-01 00:00:00

abstract：:Interleukin-1 (IL-1) is a proinflammatory cytokine released by many cell types that acts in both an autocrine and/or paracrine fashion. While IL-1 is best described as an important mediator of the peripheral immune response during infection and inflammation, increasing evidence implicates IL-1 signaling in the pathoge...

journal_title：Journal of neurochemistry

authors： Fogal B,Hewett SJ

更新日期：2008-07-01 00:00:00

abstract：:An acid-hydrolyzable conjugate of 3,4-dihydroxyphenylethylamine (dopamine, DA) was detected in superfusates from slices from rat striatum. The concentrations of endogenous free and conjugated DA, and of the acid metabolites (3,4-dihydroxyphenylacetic acid [DOPAC] and homovanillic acid [HVA]) in superfusates were measu...

journal_title：Journal of neurochemistry

authors： Tyce GM,Rorie DK

更新日期：1982-11-01 00:00:00

abstract：:Intracerebroventricular (i.c.v.) administration of angiotensin IV (Ang IV) or Leu-Val-Val-haemorphin 7 (LVV-H7) improves memory performance in normal rats and reverses memory deficits in rat models for cognitive impairment. These memory effects were believed to be mediated via the putative 'AT4 receptor'. However, thi...

journal_title：Journal of neurochemistry

authors： De Bundel D,Demaegdt H,Lahoutte T,Caveliers V,Kersemans K,Ceulemans AG,Vauquelin G,Clinckers R,Vanderheyden P,Michotte Y,Smolders I

更新日期：2010-03-01 00:00:00

abstract：:In the last 40 years, especially with the application of new neurochemical and molecular biological techniques, there has been explosive progress in understanding how certain ligands and drugs are transported across the blood-brain barrier and choroid plexus out of brain and CSF. In the CNS, there are several separate...

journal_title：Journal of neurochemistry

authors： Spector R

更新日期：2010-01-01 00:00:00

abstract：:The protein kinase C γ (PKCγ) undergoes multistep activation and participates in various cellular processes in Purkinje cells. Perturbations in its phosphorylation state, conformation or localization can disrupt kinase signalling, such as in spinocerebellar ataxia type 14 (SCA14) that is caused by missense mutations i...

journal_title：Journal of neurochemistry

authors： Jezierska J,Goedhart J,Kampinga HH,Reits EA,Verbeek DS

更新日期：2014-03-01 00:00:00

abstract：:Neuropeptide Y (NPY) elicits a plethora of physiological effects by interacting with several distinct G protein-coupled receptors. Activation of one of these receptors, the NPY Y5 receptor, is thought to result in increased food intake, anticonvulsant effects, attenuation of opiate withdrawal, inhibition of neuronal a...

journal_title：Journal of neurochemistry

authors： Parker EM,Xia L

更新日期：1999-09-01 00:00:00

abstract：:It is well established that inflammatory changes contribute to brain ageing, and an increased concentration of proinflammatory cytokine, interleukin-1beta (IL-1beta), has been reported in the aged brain associated with a deficit in long-term potentiation (LTP) in rat hippocampus. The precise age at which changes are i...

journal_title：Journal of neurochemistry

authors： Griffin R,Nally R,Nolan Y,McCartney Y,Linden J,Lynch MA

更新日期：2006-11-01 00:00:00

abstract：:Exposure to psychostimulants results in neuroadaptive changes of the mesencephalic dopaminergic system including morphological reorganization of dopaminergic neurons. Increased dendrite arborization and soma area were previously observed in primary cultures of mesencephalic dopaminergic neurons after 3-day exposure to...

journal_title：Journal of neurochemistry

authors： Collo G,Bono F,Cavalleri L,Plebani L,Merlo Pich E,Millan MJ,Spano PF,Missale C

更新日期：2012-03-01 00:00:00

abstract：:In the present study we have used perfused hippocampal slices to examine the hypothesis that nitric oxide (NO) can evoke the release of adenosine from nervous tissue. ATP stores were labeled by incubation with [3H]adenine. Electrical field stimulation at 5 Hz for 5 min evoked the release of 3H-purines, and this was en...

journal_title：Journal of neurochemistry

authors： Fallahi N,Broad RM,Jin S,Fredholm BB

更新日期：1996-07-01 00:00:00

abstract：:We examined the effects of chronic systemic administration of the mitochondrial toxin 3-nitropropionic acid (3-NP) in doses ranging from 12 to 16 mg/kg/day for 30 days on striatal cytoarchitecture in rats. Administration of 3-NP at a dose of 16 mg/kg/day resulted in large lesions with a central necrotic core that was ...

journal_title：Journal of neurochemistry

authors： Wüllner U,Young AB,Penney JB,Beal MF

更新日期：1994-11-01 00:00:00

abstract：:Microelectrophoretic methods were used to study the effects on cat spinal neurones of a number of compounds structurally related to the gamma-aminobutyric acid (GABA) agonists muscimol, THIP, and isoguvacine. While N-methylmuscimol was an agonist at bicuculline methochloride-sensitive GABA receptors, somewhat weaker t...

journal_title：Journal of neurochemistry

authors： Krogsgaard-Larsen P,Hjeds H,Curtis DR,Leah JD,Peet MJ

更新日期：1982-11-01 00:00:00

abstract：:Recently, a tyrosine hydroxylase (TH)-expressing CNS-derived cell line, CAD, was obtained that is capable of undergoing reversible morphological differentiation. The isolation of the CAD line allowed us to ask whether different DNA regulatory elements direct TH transcription when cells are growing and undifferentiated...

journal_title：Journal of neurochemistry

authors： Lazaroff M,Qi Y,Chikaraishi DM

更新日期：1998-07-01 00:00:00

abstract：:Cytokines and catecholamines are known to constitute a significant portion of the regulatory neuroimmune networks involved in maintaining homeostasis in the central nervous system (CNS). Although we have already reported an increase in norepinephrine (NE) turnover within the locus coeruleus (LC) at 2 and 4 h after the...

journal_title：Journal of neurochemistry

authors： Kaneko YS,Mori K,Nakashima A,Sawada M,Nagatsu I,Ota A

更新日期：2005-07-01 00:00:00

abstract：:We have identified and cataloged 54 genes that exhibit predominant expression in the striatum. Our hypothesis is that such mRNA molecules are likely to encode proteins that are preferentially associated with particular physiological processes intrinsic to striatal neurons, and therefore might contribute to the regiona...

journal_title：Journal of neurochemistry

authors： Desplats PA,Kass KE,Gilmartin T,Stanwood GD,Woodward EL,Head SR,Sutcliffe JG,Thomas EA

更新日期：2006-02-01 00:00:00

abstract：:Phospholipase C gamma 1 (PLC-gamma 1) is involved at an early step in signal transduction of many hormones and growth factors and catalyzes the hydrolysis of phosphatidylinositol (PI) 4,5-bisphosphate to diacylglycerol and inositol trisphosphate, two potent intracellular second messenger molecules. The transformation ...

journal_title：Journal of neurochemistry

authors： Widmer HR,Kaplan DR,Rabin SJ,Beck KD,Hefti F,Knüsel B

更新日期：1993-06-01 00:00:00

abstract：:Rats were administered [3H]fucose by intracranial injection and synaptic membranes (SMs) isolated 18 h later. Oligosaccharides associated with SM glycoproteins were prepared by hydrazinolysis and analyzed by a combination of affinity chromatography on concanavalin A (Con A)-agarose, ion-exchange chromatography on DEAE...

journal_title：Journal of neurochemistry

authors： Stanojev D,Gurd JW

更新日期：1987-05-01 00:00:00

abstract：:We have correlated myelin membrane structure with biochemical composition in the CNS and PNS of a phylogenetic series of animals, including elasmobranchs, teleosts, amphibians, and mammals. X-ray diffraction patterns were recorded from freshly dissected, unfixed tissue and used to determine the thicknesses of the liqu...

journal_title：Journal of neurochemistry

authors： Kirschner DA,Inouye H,Ganser AL,Mann V

更新日期：1989-11-01 00:00:00

abstract：:We report on the expression of ionotropic glutamate receptor subunits in primary neuronal cultures from rat cortex, hippocampus and cerebellum and of metabotropic glutamate (mGlu) receptor subtypes in these neuronal cultures as well as in cortical astroglial cultures. We found that the NMDA receptor (NR) subunits NR1,...

journal_title：Journal of neurochemistry

authors： Janssens N,Lesage AS

更新日期：2001-06-01 00:00:00

abstract：:Levels of multidrug efflux transporter P-glycoprotein (P-gp) on endothelial cells lining brain blood vessels are important for limiting access of many compounds to the brain. In vivo studies have indicated that ischaemia-reperfusion that generates reactive oxygen species also increases P-gp levels in brain endothelial...

journal_title：Journal of neurochemistry

authors： Robertson SJ,Kania KD,Hladky SB,Barrand MA

更新日期：2009-10-01 00:00:00

abstract：:Genomic and cDNA clones encoding the rat D1 receptor were isolated and sequenced. Comparison of the D1 receptor cDNA and genomic sequences revealed that the rat D1 receptor gene is organized into two exons separated by a small intron in the 5' untranslated region of its mRNA. The transcription start site is located 86...

journal_title：Journal of neurochemistry

authors： Zhou QY,Li C,Civelli O

更新日期：1992-11-01 00:00:00

abstract：:Following a previous report on detection of muscarinic receptors in myelin with the implied presence of G proteins, we now demonstrate by more direct means the presence of such proteins and their quantification. Using [35S]guanosine 5'-O-(3-thiotriphosphate) ([35S]GTP gamma S) as the binding ligand, purified myelin fr...

journal_title：Journal of neurochemistry

authors： Larocca JN,Golly F,Ledeen RW

更新日期：1991-07-01 00:00:00

abstract：:Tetanus and botulinum toxins bind and are internalized at the neuromuscular junction. Botulinum neurotoxins (BoNTs) enter the cytosol at the motor nerve terminal; tetanus neurotoxin (TeNT) proceeds retroaxonally inside the motor axon to reach the spinal cord inhibitory interneurons. Although the major target of BoNTs ...

journal_title：Journal of neurochemistry

authors： Verderio C,Coco S,Rossetto O,Montecucco C,Matteoli M

更新日期：1999-07-01 00:00:00

abstract：:Cavernous angiomas are vascular malformations that cause neurodegeneration and symptoms including epileptiform seizures, headache, and motor deficits. Following neurosurgical removal of the angiomas, patients mostly recover well and become seizure-free. This study reports on the levels of certain amino acids in angiom...

journal_title：Journal of neurochemistry

authors： von Essen C,Rydenhag B,Nyström B,Mozzi R,van Gelder N,Hamberger A

更新日期：1996-07-01 00:00:00

abstract：:Multiple sclerosis (MS) pathology is marked by the massive infiltration of myelin-specific T cells into the CNS. Hallmarks of T helper (Th) cells during active disease are pro-inflammatory Th1/Th17 cells that predominate over immunoregulatory Th2/Treg cells. Neurodegeneration, a major factor in progressive MS, is ofte...

journal_title：Journal of neurochemistry

authors： Trager N,Smith A,Wallace Iv G,Azuma M,Inoue J,Beeson C,Haque A,Banik NL

更新日期：2014-07-01 00:00:00

abstract：:The binding of the potent adenosine uptake inhibitor [3H]nitrobenzylthioinosine ([3H]NBI) to brain membrane fractions was investigated. Reversible, saturable, specific, high-affinity binding was demonstrated in both rat and human brain. The KD in both was 0.15 nM with Bmax values of 140-200 fmol/mg protein. Linear Sca...

journal_title：Journal of neurochemistry

authors： Marangos PJ,Patel J,Clark-Rosenberg R,Martino AM

更新日期：1982-07-01 00:00:00

abstract：:The potential anxiolytic effects of a novel positive allosteric modulator (PAM) of the metabotropic glutamate receptor subgroup 2 (mGluR₂) were investigated using a self-referencing recording technique with enzyme-based microelectrode arrays (MEAs) that reliably measures tonic and phasic changes in extracellular gluta...

journal_title：Journal of neurochemistry

authors： Hascup ER,Hascup KN,Pomerleau F,Huettl P,Hajos-Korcsok E,Kehr J,Gerhardt GA

更新日期：2012-08-01 00:00:00