Abstract：
:Glutamate signaling in the mature retinal tissue is very important for accurate sensory decoding by retinal neurons and orchestrates the fine-tuned output from the retina to higher-order centers at the cerebral cortex. In this study, we show that glutamate induces a rapid extracellular-regulated kinase and cAMP-responsive element binding protein (CREB) phosphorylation in cultured developing retinal neurons. This process is reliant on alpha-amino-3-hydroxy-5-methylisoxazole-4-propionate receptors and nitric oxide (NO) signaling and independent of NMDA receptors activation, as it is blocked by alpha-amino-3-hydroxy-5-methylisoxazole-4-propionate/kainate antagonists as well as inhibiting NO synthase with NG-nitro-L-arginine methyl ester but not by the NMDA channel blocker dizocilpine maleate. The effect of NO on extracellular-regulated kinase and CREB is mediated by the classical NO/soluble guanylyl cyclase/protein kinase G pathways as it is inhibited by the soluble guanylyl cyclase blocker 1H-[1,2,4]oxadiazole[4,3-a]quinoxalin-1-one and the protein kinase G inhibitor KT5823, respectively. Immunocytochemical data suggest that increased CREB phosphorylation in response to glutamate occurs in glial cell nuclei. We also have supporting evidence suggesting that neuronally produced NO directly reaches the glial cells and stimulates CREB phosphorylation. Hence, the results indicate the importance of neuronal-glial communication and glutamate/NO/CREB linkage during retinal development.
journal_name
J Neurochemjournal_title
Journal of neurochemistryauthors
Socodato RE,Magalhães CR,Paes-de-Carvalho Rdoi
10.1111/j.1471-4159.2008.05778.xsubject
Has Abstractpub_date
2009-01-01 00:00:00pages
417-29issue
2eissn
0022-3042issn
1471-4159pii
JNC5778journal_volume
108pub_type
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journal_title：Journal of neurochemistry
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pub_type： 杂志文章
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更新日期：1997-11-01 00:00:00
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pub_type： 杂志文章
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更新日期：2019-10-01 00:00:00
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journal_title：Journal of neurochemistry
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pub_type： 杂志文章
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更新日期：1995-09-01 00:00:00
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journal_title：Journal of neurochemistry
pub_type： 杂志文章
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更新日期：2011-10-01 00:00:00
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更新日期：2006-03-01 00:00:00
