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Adenosine A(1) receptor in cultured neurons from rat cerebral cortex: colocalization with adenosine deaminase.

Abstract：

:Adenosine A(1) receptors (A(1)Rs) have been characterized in primary cultures of neurons from cerebral cortex. The specific adenosine A(1) antagonist 8-cyclopentyl-1,3-[(3)H]dipropylxanthine bound to both membranes and intact cells. When saturation experiments were performed in membranes, a K(D) value of 0.76 nM and a B(max) of 57 fmol/mg of protein were obtained. Competition assays revealed a pharmacological profile characteristic of A(1)Rs. The presence of this receptor was further confirmed by RT-PCR analysis. The expression of the receptor showed no significant changes during the period of culture studied, up to 12 days in vitro. A(1)R agonist inhibited forskolin-stimulated adenylyl cyclase, showing the functional coupling of these receptors with the effector. alphaG(i1, 2) protein level, detected by immunoblot, presented an increase during the period of culture. This increase correlated with an increase in the mRNA level of alphaG(i1) but not alphaG(i2). By immunochemical assays, it is shown that these receptors are expressed in both the neuronal cell body and the proximal dendrites. Colocalization of A(1)Rs with microtubule-associated protein 2 and cell surface adenosine deaminase was shown by confocal microscopy. The high degree of colocalization observed between A(1)Rs and ectoadenosine deaminase in neurons could suggest an important role of the enzyme in adenosine-mediated neuromodulation.

journal_name

J Neurochem

journal_title

Journal of neurochemistry

authors

Ruiz MA,Escriche M,Lluis C,Franco R,Martín M,Andrés A,Ros M

doi

10.1046/j.1471-4159.2000.0750656.x

keywords:

subject

Has Abstract

pub_date

2000-08-01 00:00:00

pages

656-64

issue

2

eissn

0022-3042

issn

1471-4159

journal_volume

75

pub_type

杂志文章

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