Translational regulation of GluR2 mRNAs in rat hippocampus by alternative 3' untranslated regions.


:The glutamate receptor 2 (GluR2) subunit determines many of the functional properties of the alpha-amino-3-hydroxy-5-methylisoxazole-4-propionate subtype of glutamate receptor. The roles of untranslated regions (UTRs) in mRNA stability, transport, or translation are increasingly recognized. The 3' end of the GluR2 transcripts are alternatively processed to form a short and long 3'UTR, giving rise to two pools of GluR2 mRNA of 4 and 6 kb in length, respectively, in the mammalian brain. However, the role of these alternative 3'UTRs in GluR2 expression has not been reported. We demonstrate that in the cytoplasm of rat hippocampus, native GluR2 mRNAs bearing the long 3'UTR are mostly retained in translationally dormant complexes of ribosome-free messenger ribonucleoprotein (mRNP), whereas GluR2 transcripts bearing the short 3'UTR are predominantly associated with actively translating ribosomes. One day after pilocarpine-induced status epilepticus (SE), the levels of both long and short GluR2 transcripts were markedly decreased in rat hippocampus. However, GluR2 mRNAs bearing the long 3'-UTRs were shifted from untranslating mRNP complexes to ribosome-containing complexes after SE, pointing to a selective translational derepression of GluR2 mRNA mediated by the long 3'UTR. In Xenopus oocytes, expression of firefly luciferase reporters bearing alternative GluR2 3'UTRs confirmed that the long 3'UTR is sufficient to suppress translation. The stability of reporter mRNAs in oocytes was not significantly influenced by alternative 5' or 3'UTRs of GluR2 over the time period examined. Overall, our findings that the long 3'UTR of GluR2 mRNA alone is sufficient to suppress translation, and the evidence for seizure-induced derepression of translation of GluR2 via the long 3'UTR strongly suggests that a regulatory signaling mechanism exists that differentially targets GluR2 transcripts with alternative 3'UTRs.


J Neurochem


Irier HA,Shaw R,Lau A,Feng Y,Dingledine R




Has Abstract


2009-04-01 00:00:00














  • Regulated intramembrane proteolysis--lessons from amyloid precursor protein processing.

    abstract::Regulated intramembrane proteolysis (RIP) controls the communication between cells and the extracellular environment. RIP is essential in the nervous system, but also in other tissues. In the RIP process, a membrane protein typically undergoes two consecutive cleavages. The first one results in the shedding of its ect...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章,评审


    authors: Lichtenthaler SF,Haass C,Steiner H

    更新日期:2011-06-01 00:00:00

  • Palmitic and stearic fatty acids induce caspase-dependent and -independent cell death in nerve growth factor differentiated PC12 cells.

    abstract::Apoptotic cell death has been proposed to play a role in the neuronal loss observed following traumatic injury in the CNS and PNS. The present study uses an in vitro tissue culture model to investigate whether free fatty acids (FFAs), at concentrations comparable to those found following traumatic brain injury, trigge...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Ulloth JE,Casiano CA,De Leon M

    更新日期:2003-02-01 00:00:00

  • Splice variation in the cytoplasmic domains of myelin oligodendrocyte glycoprotein affects its cellular localisation and transport.

    abstract::Although myelin oligodendrocyte glycoprotein is a candidate autoantigen in multiple sclerosis, its function remains unknown. In humans, mRNA expressed by the myelin oligodendrocyte glycoprotein gene is alternatively spliced resulting in at least nine unique protein isoforms. In this study, we investigated the sub-cell...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Boyle LH,Traherne JA,Plotnek G,Ward R,Trowsdale J

    更新日期:2007-09-01 00:00:00

  • Novel endogenous 1,2,3,4-tetrahydroisoquinoline derivatives: uptake by dopamine transporter and activity to induce parkinsonism.

    abstract::We designed as candidate metabolites and synthesized two 1-benzyl-1,2,3,4-tetrahydroisoquinoline derivatives containing a dopamine moiety: 1-(3',4'-dihydroxybenzyl)-1,2,3,4-tetrahydroisoquinoline (3',4'DHBnTIQ) and 1-benzyl-6,7-dihydroxy-1,2,3,4-tetrahydroisoquinoline (6,7DHBnTIQ). Both were detected in mouse brain as...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Kawai H,Makino Y,Hirobe M,Ohta S

    更新日期:1998-02-01 00:00:00

  • The role of putative intragenic control elements in c-fos regulation by calcium and growth factor signalling pathways.

    abstract::Sequences in the transcribed region of the c-fos gene have been suggested to control c-fos induction following exposure of cells to mitogens or stimuli that increase intracellular calcium concentrations. Using a mutational analysis we show that putative regulatory elements present in the first intron of the human c-fo...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Lange CM,Bading H

    更新日期:2001-06-01 00:00:00

  • Biogenesis of presynaptic terminal proteins.

    abstract::The delivery of proteins to the presynaptic terminals of guinea pig retinal ganglion cells by two of the major components of axonal transport, and the subsequent persistence and turnover of those proteins were examined in this study. Ganglion cell proteins were radiolabeled by intravitreal injection of radiolabeled am...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Garner JA,Mahler HR

    更新日期:1987-09-01 00:00:00

  • The endocannabinoid system in the brain of Carassius auratus and its possible role in the control of food intake.

    abstract::Cannabinoid receptors and the endocannabinoids anandamide and 2-arachidonoylglycerol have been suggested to regulate food intake in several animal phyla. Orthologs of the mammalian cannabinoid CB(1) and CB(2) receptors have been identified in fish. We investigated the presence of this endocannabinoid system in the bra...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Valenti M,Cottone E,Martinez R,De Pedro N,Rubio M,Viveros MP,Franzoni MF,Delgado MJ,Di Marzo V

    更新日期:2005-11-01 00:00:00

  • A comparison of the signalling properties of two tyramine receptors from Drosophila.

    abstract::In invertebrates, the phenolamines, tyramine and octopamine, mediate many functional roles usually associated with the catecholamines, noradrenaline and adrenaline, in vertebrates. The α- and β-adrenergic classes of insect octopamine receptor are better activated by octopamine than tyramine. Similarly, the Tyramine 1 ...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Bayliss A,Roselli G,Evans PD

    更新日期:2013-04-01 00:00:00

  • Desensitization of muscarinic receptor-coupled phosphoinositide hydrolysis in rat hippocampus: comparisons with the alpha 1-adrenergic response.

    abstract::In the presence of lithium, carbamylcholine chloride (carbachol) and epinephrine increase the accumulation of inositol monophosphate severalfold in hippocampal slices from the rat. The stimulation by carbachol (EC50, 31 microM) is mediated by muscarinic receptors, whereas the effects of epinephrine (EC50, 2 microM) ar...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Lenox RH,Hendley D,Ellis J

    更新日期:1988-02-01 00:00:00

  • Rearrangement and reactivation of the myelin basic protein locus in myelin-deficient (mld) mouse brain.

    abstract::Myelin-deficient (mld) is a complex mutation affecting the myelin basic protein (MBP) locus of the mouse. It consists of duplication and partial inversion of the MBP gene and results in a dysfunctional MBP locus. The mutant phenotype is reversed, both in vivo and in vitro, in approximately 5% of mld oligodendrocytes. ...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Sun Y,Xu J,Barbarese E,Carson JH

    更新日期:1997-02-01 00:00:00

  • Preferential resistance of dopaminergic neurons to the toxicity of glutathione depletion is independent of cellular glutathione peroxidase and is mediated by tetrahydrobiopterin.

    abstract::Depletion of glutathione in the substantia nigra is one of the earliest changes observed in Parkinson's disease (PD) and could initiate dopaminergic neuronal degeneration. Nevertheless, experimental glutathione depletion does not result in preferential toxicity to dopaminergic neurons either in vivo or in vitro. Moreo...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Nakamura K,Wright DA,Wiatr T,Kowlessur D,Milstien S,Lei XG,Kang UJ

    更新日期:2000-06-01 00:00:00

  • Specific changes of sulfatide levels in individuals with pre-clinical Alzheimer's disease: an early event in disease pathogenesis.

    abstract::To explore the hypothesis that alterations in cellular membrane lipids are present at the stage of pre-clinical Alzheimer's disease (AD) (i.e., cognitively normal at death, but with AD neuropathology), we performed targeted shotgun lipidomics of lipid extracts from post-mortem brains of subjects with pre-clinical AD. ...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Cheng H,Wang M,Li JL,Cairns NJ,Han X

    更新日期:2013-12-01 00:00:00

  • Biochemical characterization of the nicotinic cholinergic receptors in human brain: binding of (-)-[3H]nicotine.

    abstract::(-)-[3H]Nicotine was found to bind specifically to membranes of human brains obtained at autopsy. The binding was stereospecific, (-)-nicotine being 40 times more potent than (+)-nicotine in displacing labeled (-)-nicotine. Saturation binding studies revealed the presence of two binding sites with dissociation constan...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Shimohama S,Taniguchi T,Fujiwara M,Kameyama M

    更新日期:1985-08-01 00:00:00

  • Developmental differences between soluble and membrane-bound fractions of choline-O-acetyltransferase in neonatal mouse brain.

    abstract::Three fractions (one soluble and two membrane-bound) of choline acetyltransferase (ChAT) isolated from a nerve ending fraction of mouse forebrain, which have previously been reported to differ in several biochemical and physical aspects, were also found to differ in their rates of postnatal development. At 2 days of a...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Benishin CG,Carroll PT

    更新日期:1984-09-01 00:00:00

  • Amyloid-beta peptide enhances tumor necrosis factor-alpha-induced iNOS through neutral sphingomyelinase/ceramide pathway in oligodendrocytes.

    abstract::Although accumulating evidence demonstrates that white matter degeneration contributes to pathology in Alzheimer's disease (AD), the underlying mechanisms are unknown. In order to study the roles of the amyloid-beta peptide in inducing oxidative stress damage in white matter of AD, we investigated the effects of amylo...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Zeng C,Lee JT,Chen H,Chen S,Hsu CY,Xu J

    更新日期:2005-08-01 00:00:00

  • Ca(2+) -independent vesicular catecholamine release in PC12 cells by nanomolar concentrations of Pb(2+).

    abstract::Effects of Pb(2+) on vesicular catecholamine release in intact and ionomycin-permeabilized PC12 cells were investigated using carbon fibre microelectrode amperometry. Changes in intracellular Pb(2+) and Ca(2+) were measured from indo-1 fluorescence by confocal laser scanning microscopy. Depolarization of intact cells ...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Westerink RH,Vijverberg HP

    更新日期:2002-03-01 00:00:00

  • BIT/SHPS-1 enhances brain-derived neurotrophic factor-promoted neuronal survival in cultured cerebral cortical neurons.

    abstract::Brain-derived neurotrophic factor (BDNF) activates a variety of signaling molecules to exert various functions in the nervous system, including neuronal differentiation, survival, and regulation of synaptic plasticity. Previously, we have suggested that BIT/SHPS-1 (brain immunoglobulin-like molecule with tyrosine-base...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Araki T,Yamada M,Ohnishi H,Sano SI,Hatanaka H

    更新日期:2000-10-01 00:00:00

  • Role of calmodulin and protein kinase C in astrocytic cell volume regulation.

    abstract::We investigated the role of Ca(2+)-dependent protein kinases in the regulation of astrocytic cell volume. Calmodulin (CaM) antagonists were used to inhibit CaM and thus Ca2+/CaM-dependent protein kinase. The effect of these inhibitors as well as activators and inhibitors of protein kinase C (PKC) on astrocytic volume ...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Bender AS,Neary JT,Blicharska J,Norenberg LO,Norenberg MD

    更新日期:1992-05-01 00:00:00

  • Purification and properties of high molecular weight calpastatin from bovine brain.

    abstract::Calcium-activated neutral proteases (calpains) are regulated by specific endogenous protein inhibitors, the calpastatins, which are widely distributed in mammalian tissues. Calpastatins from different species or in various tissues from the same species exhibit considerable size heterogeneity on sodium dodecyl sulfate ...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Mohan PS,Nixon RA

    更新日期:1995-02-01 00:00:00

  • Neuron-specific non-classical release of prothymosin alpha: a novel neuroprotective damage-associated molecular patterns.

    abstract::Prothymosin alpha (ProTα), a nuclear protein devoid of signal sequence, has been shown to possess a number of cellular functions including cell survival. Most recently, we demonstrated that ProTα is localized in the nuclei of neurons, while it is found in both nuclei and cytoplasm in the astrocytes and microglia of ad...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Halder SK,Matsunaga H,Ueda H

    更新日期:2012-10-01 00:00:00

  • Communication between neurons and astrocytes: relevance to the modulation of synaptic and network activity.

    abstract::Neuromodulation is a fundamental process in the brain that regulates synaptic transmission, neuronal network activity and behavior. Emerging evidence demonstrates that astrocytes, a major population of glial cells in the brain, play previously unrecognized functions in neuronal modulation. Astrocytes can detect the le...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章,评审


    authors: Fellin T

    更新日期:2009-02-01 00:00:00

  • R3F, a novel membrane-associated glycogen targeting subunit of protein phosphatase 1 regulates glycogen synthase in astrocytoma cells in response to glucose and extracellular signals.

    abstract::Abnormal regulation of brain glycogen metabolism is believed to underlie insulin-induced hypoglycaemia, which may be serious or fatal in diabetic patients on insulin therapy. A key regulator of glycogen levels is glycogen targeted protein phosphatase 1 (PP1), which dephosphorylates and activates glycogen synthase (GS)...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Kelsall IR,Voss M,Munro S,Cuthbertson DJ,Cohen PT

    更新日期:2011-08-01 00:00:00

  • Caffeine protects against MPTP-induced blood-brain barrier dysfunction in mouse striatum.

    abstract::The blood-brain barrier (BBB) is important physiologically. Pathologically, BBB disruption has been implicated in a wide spectrum of neurological disorders including Parkinson's disease (PD). Recent studies indicate that caffeine is protective against PD, but by poorly understood mechanisms. Using a MPTP neurotoxin mo...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Chen X,Lan X,Roche I,Liu R,Geiger JD

    更新日期:2008-11-01 00:00:00

  • Histamine stimulation of cyclic AMP accumulation in astrocyte-enriched and neuronal primary cultures from rat brain.

    abstract::Histamine stimulates cyclic AMP accumulation in astrocyte-enriched and neuronal primary cultures from rat brain in the presence of the phosphodiesterase inhibitor isobutylmethylxanthine. The response in the astrocyte cultures (Emax = 304 +/- 44% over basal, EC50 = 43 +/- 5 microM) was much higher than in neuronal cult...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Agulló L,Picatoste F,García A

    更新日期:1990-11-01 00:00:00

  • Selective visualization of rodent locus ceruleus by a radiolabeled N-methyl-4-phenyl-1,2,3,6-tetrahydropyridine analog.

    abstract::The neurotoxic metabolite of N-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), 1-methyl-4-phenylpyridinium, selectively accumulates in dopaminergic neurons via the dopamine reuptake system. Consequently, nontoxic radiolabeled MPTP analogs may be potentially useful for visualizing catecholaminergic neurons in vivo. ...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Efange SM,Mash D,Hefti F,Kung HF,Billings J

    更新日期:1989-08-01 00:00:00

  • p-ethynylphenylalanine: a potent inhibitor of tryptophan hydroxylase.

    abstract::Tryptophan hydroxylase (TPH) is the initial and rate-limiting enzyme in serotonin biosynthesis. The enzyme activity is dependent on molecular oxygen, a tetrahydropterin cosubstrate, and ferrous iron. The present study demonstrates that TPH is inhibited by a novel compound, p-ethynylphenylalanine (pEPA), produced by th...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Stokes AH,Xu Y,Daunais JA,Tamir H,Gershon MD,Butkerait P,Kayser B,Altman J,Beck W,Vrana KE

    更新日期:2000-05-01 00:00:00

  • Regulation of striatal nitric oxide synthesis by local dopamine and glutamate interactions.

    abstract::Nitric oxide (NO) is a key neuromodulator of corticostriatal synaptic transmission. We have shown previously that dopamine (DA) D1/5 receptor stimulation facilitates neuronal NO synthase (nNOS) activity in the intact striatum. To study the impact of local manipulations of D1/5 and glutamatergic NMDA receptors on stria...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Park DJ,West AR

    更新日期:2009-12-01 00:00:00

  • Therapeutic approaches in Parkinson's disease and related disorders.

    abstract::The lack of effective therapies for neurodegenerative disorders is one of the most relevant challenges of this century, considering that, as the global population ages, the incidence of these type of diseases is quickly on the rise. Among these disorders, synucleinopathies, which are characterized by the abnormal accu...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章,评审


    authors: Valera E,Masliah E

    更新日期:2016-10-01 00:00:00

  • Brain beta-spectrin phosphorylation: phosphate analysis and identification of threonine-347 as a heparin-sensitive protein kinase phosphorylation site.

    abstract::Phosphorylation of brain spectrin was studied by a combination of in vivo and in vitro approaches. Chemical analysis of phosphate groups on electrophoretically purified mouse brain beta-spectrin yielded a stoichiometry of 3.2 +/- 0.18 mol of PO4/mol of beta-spectrin. The spectrin isolated by chromatographic methods fr...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Sihag RK

    更新日期:1998-11-01 00:00:00

  • 2'-Deoxycoformycin inhibition of adenosine deaminase in rat brain: in vivo and in vitro analysis of specificity, potency, and enzyme recovery.

    abstract::2'-Deoxycoformycin (DCF), a potent inhibitor of adenosine deaminase (ADA), is increasingly used as a tool to investigate adenosine metabolism and neuromodulation. To advance further the usefulness of DCF for studies of purines in the CNS, we determined the inhibitory potency of this compound against ADA and adenylate ...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Padua R,Geiger JD,Dambock S,Nagy JI

    更新日期:1990-04-01 00:00:00