Cryptococcal capsular polysaccharide clearance in nonimmune mice.

Abstract:

:Clearance of cryptococcal polysaccharide (CP) from tissues and body fluids of nonimmune mice was studied. Mice were injected intravenously only with one mg of purified CP, and serum, urine and tissues were obtained from each animal at various intervals for a period of 84 days. Tissue extracts, serum and urine were tested for CP content by enzyme-linked immunosorbent assay (ELISA) and latex agglutination. High concentrations of CP were detected by both assays one-half hour after injection in blood (serum), liver, spleen, kidney and lung (extracts). The duration of ELISA detectable CP was longest (70 days) in liver and spleen and shortest (14 days) in lung extract. By 14 days after injection, concentration of CP in the blood fell below that found in the liver and spleen. CP remained detectable (titers 32-64) after all other extracts became negative. These results indicate that CP is stored in tissues (binding mechanism and site unknown), and that the liver and spleen possess greater storage capacity than other tissues. Antibody (IgM) to CP appeared in low titer on the 14th day and thereafter.

journal_name

Mycopathologia

journal_title

Mycopathologia

authors

Muchmore HG,Scott EN,Felton FG,Fromtling RA

doi

10.1007/BF00436580

subject

Has Abstract

pub_date

1982-04-23 00:00:00

pages

41-5

issue

1

eissn

0301-486X

issn

1573-0832

journal_volume

78

pub_type

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