Human platelet-derived growth factor stimulates amino acid transport and protein synthesis by human diploid fibroblasts in plasma-free media.

abstract：:Endothelial cells (EC) play a central role in inflammatory immune responses and efficiently induce effector functions in T cells, despite lacking the classical costimulatory ligands CD80 and CD86. By using the mAb HIL-131 we now demonstrate that human inducible costimulator-ligand (ICOS-L), a molecule related to CD80/...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Khayyamian S,Hutloff A,Büchner K,Gräfe M,Henn V,Kroczek RA,Mages HW

更新日期：2002-04-30 00:00:00

abstract：:We derive more than 80 embeddings of 2D hyperbolic honeycombs in Euclidean 3 space, forming 3-periodic infinite polyhedra with cubic symmetry. All embeddings are "minimally frustrated," formed by removing just enough isometries of the (regular, but unphysical) 2D hyperbolic honeycombs [Formula: see text], [Formula: se...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pedersen MC,Hyde ST

更新日期：2018-07-03 00:00:00

abstract：:In recent years, considerable progress has been made in understanding the molecular mechanisms underlying olfaction in insects. Because of the diverse nature of the gene families involved, this process has largely relied on genomic data. As a consequence, studies have focused on a small subset of species with extensiv...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Grosse-Wilde E,Kuebler LS,Bucks S,Vogel H,Wicher D,Hansson BS

更新日期：2011-05-03 00:00:00

abstract：:Examination of the structural basis for antiviral activity, oral pharmacokinetics, and hepatic metabolism among a series of symmetry-based inhibitors of the human immunodeficiency virus (HIV) protease led to the discovery of ABT-538, a promising experimental drug for the therapeutic intervention in acquired immunodefi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kempf DJ,Marsh KC,Denissen JF,McDonald E,Vasavanonda S,Flentge CA,Green BE,Fino L,Park CH,Kong XP

更新日期：1995-03-28 00:00:00

abstract：:A fraction of the unintegrated viral DNA that appears early after infection of mouse cells by Moloney leukemia virus is infectious. The infectivity could be deomonstrated by an XC plaque assay of the cells exposed to DNA co-precipitated with calcium phosphate. The number of plaques deriving from closed-circular, super...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Smotkin D,Gianni AM,Rozenblatt S,Weinberg RA

更新日期：1975-12-01 00:00:00

abstract：:Several of the best-studied sex differences in the mammalian brain are ascribed to the hormonal control of cell death. This conclusion is based primarily on correlations between pyknotic cell counts in development and counts of mature neurons in adulthood; the molecular mechanisms of hormone-regulated, sexually dimorp...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Forger NG,Rosen GJ,Waters EM,Jacob D,Simerly RB,de Vries GJ

更新日期：2004-09-14 00:00:00

abstract：:The cytosolic concentration of free Ca2+ in bovine neutrophils was monitored by using the intracellular Ca2+ indicator quin2, 2-[[2-bis(acetylamino)-5-methylphenoxy]methyl-6-methoxy-8- bis(acetylamino)]quinoline. Neutrophils at rest have a cytosolic Ca2+ concentration of 85 +/- 5 nM, which in 2-4 min increases to 300-...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gennaro R,Pozzan T,Romeo D

更新日期：1984-03-01 00:00:00

abstract：:In contrast to mechanisms mediating uptake of intracellular bacterial pathogens, bacterial egress and cell-to-cell transmission are poorly understood. Previously, we showed that the transmission of pathogenic mycobacteria between phagocytic cells also depends on nonlytic ejection through an F-actin based structure, ca...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gerstenmaier L,Pilla R,Herrmann L,Herrmann H,Prado M,Villafano GJ,Kolonko M,Reimer R,Soldati T,King JS,Hagedorn M

更新日期：2015-02-17 00:00:00

abstract：:Gene-regulatory networks are ubiquitous in nature and critical for bottom-up engineering of synthetic networks. Transcriptional repression is a fundamental function that can be tuned at the level of DNA, protein, and cooperative protein-protein interactions, necessitating high-throughput experimental approaches for in...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Swank Z,Laohakunakorn N,Maerkl SJ

更新日期：2019-03-26 00:00:00

abstract：:Bathorhodopsin-rhodopsin difference spectra of native 11-cis-rhodopsin and regenerated 9-cis-rhodopsin were measured at room temperature with a double-beam laser spectrophotometer after excitation at 532 nm. A detailed analysis of data obtained at 85 psec after excitation suggests that the bathorhodopsins generated fr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Spalink JD,Reynolds AH,Rentzepis PM,Sperling W,Applebury ML

更新日期：1983-04-01 00:00:00

abstract：:The roles of superoxide and H2O2 in the cytotoxicity of paraquat were assessed in Chinese hamster ovary cells. Neither catalase nor superoxide dismutase inhibited the loss of ability to form colonies when added to the medium. When introduced into the cells, superoxide dismutase but not catalase inhibited the toxicity ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bagley AC,Krall J,Lynch RE

更新日期：1986-05-01 00:00:00

abstract：:GTP-tubulin forms a cap on microtubule ends during aggregation. The bulk of the microtubule is GDP-tubulin. This complicates the usual simple kinetic theory of subunit exchange at microtubule ends to such an extent that Monte Carlo calculations are needed to handle the complications, except in special cases. The Monte...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen Y,Hill TL

更新日期：1983-12-01 00:00:00

abstract：:The alpha and beta subunits of Na-K-ATPase are up-regulated by hypertonicity in inner-medullary collecting duct cells adapted to survive in hypertonic conditions. We examined the regulation of the gamma subunit by hypertonicity. Although cultured inner-medullary collecting duct cells lacked the gamma subunits, both va...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Capasso JM,Rivard C,Berl T

更新日期：2001-11-06 00:00:00

abstract：:Somatic mutation and neoplastic transformation of diploid Syrian hamster embryo cells were examined concomitantly. Mutations induced by benzo[a]pyrene and N-methyl-N'-nitro-N-nitrosoguanidine were quantitated at the hypoxanthine phosphoribosyltransferase and Na(+)/K(+) ATPase loci and compared to phenotypic transforma...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Barrett JC,Ts'o PO

更新日期：1978-07-01 00:00:00

abstract：:In eukaryotes, RNA polymerase I transcription is controlled by DNA elements located within the spacers that separate the tandemly arranged rRNA genes. Unlike rRNA coding sequences, the intergenic spacers evolve rapidly and have little sequence similarity even among closely related species. Nonetheless, the arrangement...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Doelling JH,Gaudino RJ,Pikaard CS

更新日期：1993-08-15 00:00:00

abstract：:The proinflammatory cytokine interleukin 1 (IL-1) activates the transcription of many genes encoding acute phase and proinflammatory proteins, a function mediated primarily by the transcription factor NF-kappaB. An early IL-1 signaling event is the recruitment of the Ser/Thr kinase IRAK to the type I IL-1 receptor (IL...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Huang J,Gao X,Li S,Cao Z

更新日期：1997-11-25 00:00:00

abstract：:Guamanian amyotrophic lateral sclerosis (ALS-G) and parkinsonism dementia (PD-G) have been epidemiologically linked to an environment severely deficient in calcium (Ca2+) and magnesium (Mg2+). Transient receptor potential melastatin 7 (TRPM7) is a bifunctional protein containing both channel and kinase domains that ha...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hermosura MC,Nayakanti H,Dorovkov MV,Calderon FR,Ryazanov AG,Haymer DS,Garruto RM

更新日期：2005-08-09 00:00:00

abstract：:We explored the detection of genome-wide hypomethylation in plasma using shotgun massively parallel bisulfite sequencing as a marker for cancer. Tumor-associated copy number aberrations (CNAs) could also be observed from the bisulfite DNA sequencing data. Hypomethylation and CNAs were detected in the plasma DNA of pat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chan KC,Jiang P,Chan CW,Sun K,Wong J,Hui EP,Chan SL,Chan WC,Hui DS,Ng SS,Chan HL,Wong CS,Ma BB,Chan AT,Lai PB,Sun H,Chiu RW,Lo YM

更新日期：2013-11-19 00:00:00

abstract：:Investigations using the fruit fly Drosophila melanogaster have shown that the circadian clock gene period (Per) can influence behavioral responses to cocaine. Here we show that the mouse homologues of the Drosophila Per gene, mPer1 and mPer2, modulate cocaine sensitization and reward, two phenomena extensively studie...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Abarca C,Albrecht U,Spanagel R

更新日期：2002-06-25 00:00:00

abstract：:Gene knockout is the most powerful tool for determining gene function or permanently modifying the phenotypic characteristics of a cell. Existing methods for gene disruption are limited by their efficiency, time to completion, and/or the potential for confounding off-target effects. Here, we demonstrate a rapid single...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Santiago Y,Chan E,Liu PQ,Orlando S,Zhang L,Urnov FD,Holmes MC,Guschin D,Waite A,Miller JC,Rebar EJ,Gregory PD,Klug A,Collingwood TN

更新日期：2008-04-15 00:00:00

abstract：:Clinically available drugs active against Epstein-Barr virus (EBV) and other human herpesviruses are limited to those targeting viral DNA replication. To identify compounds directed against other steps in the viral life cycle, we searched for drugs active against the EBV SM protein, which is essential for infectious v...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Verma D,Thompson J,Swaminathan S

更新日期：2016-03-29 00:00:00

abstract：:In the usual Darwinian case in which struggle for existence leads to density limitations on the environment's carrying capacity, R. A. Fisher's reproductive-value concept reduces to zero for every initial age group. To salvage some meaning for Fisher's notion, two variant reproductive-value concepts are defined here: ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Samuelson PA

更新日期：1978-12-01 00:00:00

abstract：:Concepts organize the relationship among individual stimuli or events by highlighting shared features. Often, new goals require updating conceptual knowledge to reflect relationships based on different goal-relevant features. Here, our aim is to determine how hippocampal (HPC) object representations are organized and ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mack ML,Love BC,Preston AR

更新日期：2016-11-15 00:00:00

abstract：:The membrane attack complex of complement is a fusion product of five complement proteins: C5b, C6, C7, C8, and C9. The complex causes complement-dependent cell membrane damage. It is assembled following complement activation both on the target cell surface and in the fluid phase. The isolated soluble complex, which h...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kolb WP,Müller-Eberhard HJ

更新日期：1975-05-01 00:00:00

abstract：:The immunity protein to colicin A (Cai), which is constitutively expressed at a very low level in Escherichia coli strains, has been studied in recombinant plasmid constructs allowing expression of various immunity fusion proteins under the control of inducible promoters. The 13-amino acid NH2-terminal region of Cai w...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Geli V,Baty D,Lazdunski C

更新日期：1988-02-01 00:00:00

abstract：:Attending to a stimulus is known to enhance the neural responses to that stimulus. Recent experiments on visual attention have shown that this modulation can have object-based characteristics, such that, when certain parts of a visual object are attended, other parts automatically also receive enhanced processing. Her...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Busse L,Roberts KC,Crist RE,Weissman DH,Woldorff MG

更新日期：2005-12-20 00:00:00

abstract：:Lysine to methionine (K-to-M) mutations in genes encoding histone H3 are thought to drive a subset of pediatric brain and bone cancers. These high-frequency K-to-M mutations occur at sites of methylation on histone H3, and tumors containing the mutant histones exhibit a global loss of specific histone methylation mark...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jayaram H,Hoelper D,Jain SU,Cantone N,Lundgren SM,Poy F,Allis CD,Cummings R,Bellon S,Lewis PW

更新日期：2016-05-31 00:00:00

abstract：:Parathyroid hormone (PTH) is central to calcium homeostasis and bone maintenance in vertebrates, and as such it has been used for treating osteoporosis. It acts primarily by binding to its receptor, PTH1R, a member of the class B G protein-coupled receptor (GPCR) family that also includes receptors for glucagon, calci...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pioszak AA,Xu HE

更新日期：2008-04-01 00:00:00

abstract：:In normal vision, shifts of attention are usually followed by saccadic eye movements. Neurons in extrastriate area V4 are modulated by focal attention when eye movements are withheld, but they also respond in advance of visually guided saccadic eye movements. We have examined the visual selectivity of saccade-related ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Moore T,Tolias AS,Schiller PH

更新日期：1998-07-21 00:00:00

abstract：:The epidermal growth factor (EGF) receptor gene is the cellular homolog of the avian erythroblastosis virus erbB oncogene. Control of EGF receptor expression determines cellular responsiveness to EGF and might play an important role in neoplastic development. Using RNA blot hybridization, we have found that exposure o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Clark AJ,Ishii S,Richert N,Merlino GT,Pastan I

更新日期：1985-12-01 00:00:00