White lines in L-edge x-ray absorption spectra and their implications for anomalous diffraction studies of biological materials.

Abstract:

:We have measured high-resolution x-ray absorption spectra of lanthanide (Ln) and heavy transition metal complexes that display prominent narrow absorption peaks near the L2 and L3 absorption edges. The anomalous scattering factors (f' and f"), which are mathematically related to the absorption cross section, have correspondingly sharp changes in their magnitude within 5-10 eV of the absorption edge. Calculations of the magnitude of the change in f' and f" demonstrate that significant changes (on the order of 20 electrons in f') can be expected for these materials. These substantial changes in the anomalous scattering factors have applications to deriving structural information for macromolecules from x-ray diffraction studies. The magnitude of the changes indicate that the anomalous scattering technique is a powerful means of obtaining structural characteristics for macromolecules in single crystals, in solution, and in biological membranes.

authors

Lye RC,Phillips JC,Kaplan D,Doniach S,Hodgson KO

doi

10.1073/pnas.77.10.5884

subject

Has Abstract

pub_date

1980-10-01 00:00:00

pages

5884-8

issue

10

eissn

0027-8424

issn

1091-6490

journal_volume

77

pub_type

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