Observations on N alpha-deacetylation of model amino acids and peptides: distribution and purification of a specific N-acyl amino acid releasing enzyme in rat brain.

abstract：:This review examines the involvement of nitrotyrosine as a marker for peroxynitrite-mediated damage in the dopamine neuronal system. We propose that the dopamine neuronal phenotype can influence the cytotoxic signature of peroxynitrite. Dopamine and tetrahydrobiopterin are concentrated in dopamine neurons, and both ar...

journal_title：Journal of neurochemistry

authors： Kuhn DM,Sakowski SA,Sadidi M,Geddes TJ

更新日期：2004-05-01 00:00:00

abstract：:The effect of docosahexaenoic acid (DHA) on neuronal survival was studied in cultured cells isolated from newborn rat retina. In vivo, the content of DHA in the retina increased nearly fourfold from days 2 to 12 after birth, whereas in retinal cells in culture it remained constant. Unlike amacrine cells, the photorece...

journal_title：Journal of neurochemistry

authors： Rotstein NP,Aveldaño MI,Barrantes FJ,Politi LE

更新日期：1996-05-01 00:00:00

abstract：:Thymosin beta 4 (Tβ4), a secreted 43 amino acid peptide, promotes oligodendrogenesis, and improves neurological outcome in rat models of neurologic injury. We demonstrated that exogenous Tβ4 treatment up-regulated the expression of the miR-200a in vitro in rat brain progenitor cells and in vivo in the peri-infarct are...

journal_title：Journal of neurochemistry

authors： Santra M,Chopp M,Santra S,Nallani A,Vyas S,Zhang ZG,Morris DC

更新日期：2016-01-01 00:00:00

abstract：:Aging was associated with an increase in the density of specific binding sites for [3H]imipramine in postmortem specimens of human hypothalamus, frontal cortex, and parietal cortex. In general, [3H]imipramine binding was not affected by factors considered difficult to control in postmortem studies, i.e., time from dea...

journal_title：Journal of neurochemistry

authors： Severson JA,Marcusson JO,Osterburg HH,Finch CE,Winblad B

更新日期：1985-11-01 00:00:00

abstract：:The content of 1,2-diacylglycerol (DAG) was determined in sciatic nerves from normal and streptozotocin-induced diabetic rats. In nerves frozen in situ, DAG content was reduced 22% in the proximal region and 77% in the distal region of diabetic nerve, principally because of the loss of associated fat. DAG levels in fr...

journal_title：Journal of neurochemistry

authors： Zhu X,Eichberg J

更新日期：1990-09-01 00:00:00

abstract：:Functional activation of NMDA receptors requires co-activation of glutamate- and glycine-binding sites. D-serine is considered to be an endogenous ligand for the glycine site of NMDA receptors. Using a combination of a rat formalin-induced conditioned place avoidance (F-CPA) behavioral model and whole-cell patch-clamp...

journal_title：Journal of neurochemistry

authors： Ren WH,Guo JD,Cao H,Wang H,Wang PF,Sha H,Ji RR,Zhao ZQ,Zhang YQ

更新日期：2006-03-01 00:00:00

abstract：:Niemann Pick type C (NPC1) is a rare fatal hereditary cholesterol storage disease associated with a massive Purkinje cells loss. The mechanisms leading to neurodegeneration are still poorly understood. Different laboratories pointed to hypersensitivity to cytotoxic effects of statins (HMG-CoA reductase inhibitors) in ...

journal_title：Journal of neurochemistry

authors： Marschalek N,Albert F,Afshordel S,Meske V,Eckert GP,Ohm TG

更新日期：2015-04-01 00:00:00

abstract：:Five muscarinic receptor genes (m1-m5) that encode distinct muscarinic receptor subtypes have been cloned. Because of their structural homology and pharmacological similarity, ligand binding probes currently available do not clearly distinguish among the subtypes. To obtain a clear distribution within the CNS of molec...

journal_title：Journal of neurochemistry

authors： Wei J,Walton EA,Milici A,Buccafusco JJ

更新日期：1994-09-01 00:00:00

abstract：:Phosphodiesterase (PDE) 7B, a cAMP-specific PDE which is dominantly expressed in striatum, is expected to be involved in dopaminergic signaling in striatal neurons. Here we show, for the first time, the involvement of the dopaminergic signaling pathway in transcriptional activation of rat PDE7B in primary striatal cul...

journal_title：Journal of neurochemistry

authors： Sasaki T,Kotera J,Omori K

更新日期：2004-04-01 00:00:00

abstract：:A recent study has linked the butyrylcholinesterase (BChE) K-variant and the apolipoprotein epsilon4 isoform to late-onset Alzheimer's disease. These findings have been controversial and have led us to examine the differences between wild-type and K-variant BChE in enzyme activity, protein stability, and quaternary st...

journal_title：Journal of neurochemistry

authors： Altamirano CV,Bartels CF,Lockridge O

更新日期：2000-02-01 00:00:00

abstract：:The extracellular regulated kinases (ERK) 1 and ERK2 are members of mitogen-activated protein (MAP) kinase family that play an important role in transducing extracellular signals to the nucleus and have been implicated in a broad spectrum of biological responses. To test the hypothesis that MAP kinases may be involved...

journal_title：Journal of neurochemistry

authors： Dwivedi Y,Rizavi HS,Roberts RC,Conley RC,Tamminga CA,Pandey GN

更新日期：2001-05-01 00:00:00

abstract：:Prodynorphin, a multifunctional precursor of several important opioid peptides, is expressed widely in the CNS. It is processed at specific single and paired basic sites to generate various biologically active products. Among the prohormone convertases (PCs), PC1 and PC2 are expressed widely in neuroendocrine tissues ...

journal_title：Journal of neurochemistry

authors： Berman Y,Mzhavia N,Polonskaia A,Furuta M,Steiner DF,Pintar JE,Devi LA

更新日期：2000-10-01 00:00:00

abstract：:Syntaxin, a protein required for the docking of synaptic vesicles, may be involved in the manifestation of synaptic plasticity. The possible involvement of syntaxin in epileptogenesis was investigated by assessing the expression levels of syntaxin1B/GR33 mRNA by in situ hybridization at different stages of hippocampal...

journal_title：Journal of neurochemistry

authors： Kamphuis W,Smirnova T,Hicks A,Hendriksen H,Mallet J,Lopes da Silva FH

更新日期：1995-11-01 00:00:00

abstract：:Potassium-induced depolarization of synaptoneurosomes prepared from rat telencephalon was found to increase [3H]amino-3-hydroxy-5-methylisoxazole-4-propionate ([3H]AMPA) binding to the AMPA receptor. The effect required the presence of calcium because it was blocked by the calcium chelator EGTA but was not blocked by ...

journal_title：Journal of neurochemistry

authors： Bernard J,Massicotte G,Baudry M

更新日期：1992-01-01 00:00:00

abstract：:The NMDA receptor/channel has been shown to be inhibited by ethanol in the clinically relevant range 25-100 mM. We studied heteromeric assemblies (NR1b/NR2) of the NMDA receptor, expressed in oocytes, to address three questions regarding this inhibition, and discovered the following: (1) The inhibition was nearly equi...

journal_title：Journal of neurochemistry

authors： Chu B,Anantharam V,Treistman SN

更新日期：1995-07-01 00:00:00

abstract：:Ten-minute incubation with 1 mM S-nitroso-L-cysteine (NO-CYS), a nitric oxide (NO) generator, induced a 3.5-fold increase in the basal dopamine (DA) efflux from rat striatal slices. Reduced hemoglobin (100 microM), a NO scavenger, blocked this response. Nomifensine (10 microM), an inhibitor of high-affinity DA transpo...

journal_title：Journal of neurochemistry

authors： Lonart G,Johnson KM

更新日期：1994-12-01 00:00:00

abstract：:Dual-probe microdialysis was used to study interstitial diffusion in the rat brain. A radiolabelled tracer, (3H]mannitol, was continuously infused at different concentrations via a probe acutely implanted into the striatum of an anaesthetized male rat or into a dilute agar gel. Samples were collected by a second probe...

journal_title：Journal of neurochemistry

authors： Höistad M,Chen KC,Nicholson C,Fuxe K,Kehr J

更新日期：2002-04-01 00:00:00

abstract：:Synaptosomes and mitochondria were isolated from the brains of warm-adapted, hibernating, and cold-acclimated golden hamsters (Mesocricetus auratus). Lipid extracts of these subcellular fractions were prepared and assayed for plasmenylethanolamine (ethanolamine plasmalogen) and cholesterol levels. The ganglioside comp...

journal_title：Journal of neurochemistry

authors： Demediuk P,Moscatelli EA

更新日期：1983-04-01 00:00:00

abstract：:We investigated the role of mitogen-activated protein kinase (MAPK) pathways in hypoxic neuronal injury using primary cultures from murine cerebral cortex. Hypoxia caused the death of approximately 50% of neurons at 16 h and approximately 65% of neurons at 24 h. This was associated with phospho-activation of the MAPK/...

journal_title：Journal of neurochemistry

authors： Jin K,Mao XO,Zhu Y,Greenberg DA

更新日期：2002-01-01 00:00:00

abstract：:The neurotransmitter glutamate, once released into the synaptic cleft, is largely recycled by the glutamate-glutamine cycle, which involves uptake into astrocytes, conversion into glutamine and subsequent release of glutamine from astrocytes as a precursor for neuroneal glutamate synthesis. We analysed glutamine efflu...

journal_title：Journal of neurochemistry

authors： Deitmer JW,Bröer A,Bröer S

更新日期：2003-10-01 00:00:00

abstract：:Parkinson's disease (PD) motor symptoms are caused by degeneration of nigrostriatal dopaminergic (DAergic) neurons. The most common causes of hereditary PD are mutations in the PARKIN gene. The ubiquitin ligase parkin has been shown to mediate neuroprotection in cell culture and in vivo, but the molecular mechanisms a...

journal_title：Journal of neurochemistry

authors： Hasegawa T,Treis A,Patenge N,Fiesel FC,Springer W,Kahle PJ

更新日期：2008-06-01 00:00:00

abstract：:Adrenomedullin, originally discovered from pheochromocytoma, is a member of the calcitonin gene-related peptide family. The production and secretion of adrenomedullin by cultured human astrocytes were studied by northern blot analysis and radioimmunoassay. Northern blot analysis showed the expression of adrenomedullin...

journal_title：Journal of neurochemistry

authors： Takahashi K,Nakayama M,Totsune K,Murakami O,Sone M,Kitamuro T,Yoshinoya A,Shibahara S

更新日期：2000-01-01 00:00:00

abstract：:The objective of the present study was to assess metabolic changes in the neocortex and hippocampus of well-oxygenated or moderately hypoxic rats in which fluorothyl-induced seizures were sustained for 5 or 20 min, or which were allowed recovery periods of 5, 15, or 45 min following cessation of 20-min seizure activit...

journal_title：Journal of neurochemistry

authors： Folbergrová J,Ingvar M,Nevander G,Siesjö BK

更新日期：1985-05-01 00:00:00

abstract：:Lysophosphatidic acid receptor (LPA(1)) signaling initiates neuropathic pain through demyelination of the dorsal root (DR). Although LPA is found to cause down-regulation of myelin proteins underlying demyelination, the detailed mechanism remains to be determined. In the present study, we found that a single intrathec...

journal_title：Journal of neurochemistry

authors： Xie W,Uchida H,Nagai J,Ueda M,Chun J,Ueda H

更新日期：2010-05-01 00:00:00

abstract：:Primary cultures of astrocytes from newborn rat brain showed evidence of a substrate-saturable process for glucose transport. The system shows a relatively high affinity for the substrate, with an apparent Km of approximately 1 mM. Maintenance of the cells in medium containing thyroid-hormone-free serum for 3, 6, or 9...

journal_title：Journal of neurochemistry

authors： Roeder LM,Williams IB,Tildon JT

更新日期：1985-11-01 00:00:00

abstract：:Choline acetyltransferase (ChAT), the enzyme synthesizing acetylcholine, is known to be activated by brain derived neurotrophic factor (BDNF). We found that the specific removal of the carbohydrate polysialic acid (PSA) significantly increased BDNF-induced ChAT-activity in embryonic septal neurons. Using a p75 neurotr...

journal_title：Journal of neurochemistry

authors： Burgess A,Aubert I

更新日期：2006-11-01 00:00:00

abstract：:Phospholipase A2 (PLA2) is the name for the class of lipolytic enzymes that hydrolyze the acyl group from the sn-2 position of glycerophospholipids, generating free fatty acids and lysophospholipids. The products of the PLA2-catalyzed reaction can potentially act as second messengers themselves, or be further metaboli...

journal_title：Journal of neurochemistry

authors： Farooqui AA,Yang HC,Rosenberger TA,Horrocks LA

更新日期：1997-09-01 00:00:00

abstract：:Myelin proteolipid protein (PLP) is known to contain long-chain, covalently bound fatty acids. Previous studies, including our own, have suggested the occurrence of an oxyester type of linkage between fatty acids and PLP. However, we found that protein-SH groups are required in the acylation reaction, suggesting the p...

journal_title：Journal of neurochemistry

authors： Bizzozero OA,Good LK

更新日期：1990-12-01 00:00:00

abstract：:The consequences of H(2)O(2)/Fe(2+)-induced oxidative stress on translocation of ethanolamine phosphoglyceride (EPG) and serine phosphoglyceride (SPG) were studied in an oligodendroglia-like cell line (OLN 93) following 3 days of supplementation with 0.1 mM docosahexaenoic acid (DHA) and a series of polar head group p...

journal_title：Journal of neurochemistry

authors： Brand A,Yavin E

更新日期：2001-09-01 00:00:00

abstract：:Adenosine A(1) receptors (A(1)Rs) have been characterized in primary cultures of neurons from cerebral cortex. The specific adenosine A(1) antagonist 8-cyclopentyl-1,3-[(3)H]dipropylxanthine bound to both membranes and intact cells. When saturation experiments were performed in membranes, a K(D) value of 0.76 nM and a...

journal_title：Journal of neurochemistry

authors： Ruiz MA,Escriche M,Lluis C,Franco R,Martín M,Andrés A,Ros M

更新日期：2000-08-01 00:00:00