Abstract：
:Adrenomedullin, originally discovered from pheochromocytoma, is a member of the calcitonin gene-related peptide family. The production and secretion of adrenomedullin by cultured human astrocytes were studied by northern blot analysis and radioimmunoassay. Northern blot analysis showed the expression of adrenomedullin mRNA in cultured human astrocytes. Immunoreactive adrenomedullin concentrations in the culture medium were 29.6+/-1.2 fmol/10(5) cells/24 h (mean +/- SEM, n = 4). Treatment with interferon-gamma (100 U/ml), tumor necrosis factor-alpha (1 and 10 ng/ml), or interleukin-1beta (1 and 10 ng/ml) for 24 h caused >20-fold increases in immunoreactive adrenomedullin levels in the culture medium of human astrocytes. On the other hand, northern blot analysis showed only small increases (approximately 40%) in the adrenomedullin mRNA expression of human astrocytes with either 100 U/ml interferon-gamma or 10 ng/ml interleukin-1beta and no noticeable change with tumor necrosis factor-alpha. Reverse phase HPLC of the medium extracts of human astrocytes treated with interferon-gamma, tumor necrosis factor-alpha, or interleukin-1beta showed that most of immunoreactive adrenomedullin was eluted in the position of adrenomedullin-(1-52). On the other hand, immunoreactive adrenomedullin in the medium of human astrocytes without cytokine treatment was eluted earlier than the adrenomedullin standard, suggesting that this immunoreactive adrenomedullin represents adrenomedullin with some modifications or fragments of the adrenomedullin precursor. The present study has shown the production and secretion of adrenomedullin by human astrocytes and increased secretion of adrenomedullin by cytokines.
journal_name
J Neurochemjournal_title
Journal of neurochemistryauthors
Takahashi K,Nakayama M,Totsune K,Murakami O,Sone M,Kitamuro T,Yoshinoya A,Shibahara Sdoi
10.1046/j.1471-4159.2000.0740099.xkeywords:
subject
Has Abstractpub_date
2000-01-01 00:00:00pages
99-103issue
1eissn
0022-3042issn
1471-4159journal_volume
74pub_type
杂志文章abstract：:Several lines of evidence implicate excitotoxic mechanisms in the pathogenesis of amyotrophic lateral sclerosis (ALS). Transgenic mice with a superoxide dismutase mutation (G93A) have been utilized as an animal model of familial ALS (FALS). We examined the cortical concentrations of glutamate using in vivo microdialys...
journal_title：Journal of neurochemistry
pub_type： 杂志文章
doi：10.1046/j.1471-4159.2001.00188.x
更新日期：2001-04-01 00:00:00
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pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：Journal of neurochemistry
pub_type： 杂志文章
doi：10.1111/j.1471-4159.1981.tb00491.x
更新日期：1981-08-01 00:00:00
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pub_type： 杂志文章
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更新日期：1985-08-01 00:00:00
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pub_type： 杂志文章
doi：10.1111/j.1471-4159.2006.04136.x
更新日期：2006-11-01 00:00:00
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pub_type： 杂志文章
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更新日期：2002-05-01 00:00:00
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journal_title：Journal of neurochemistry
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更新日期：2014-04-01 00:00:00
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pub_type： 杂志文章
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journal_title：Journal of neurochemistry
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pub_type： 杂志文章
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更新日期：2013-09-01 00:00:00
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pub_type： 杂志文章
doi：10.1046/j.1471-4159.1996.67010011.x
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更新日期：2016-12-01 00:00:00
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journal_title：Journal of neurochemistry
pub_type： 杂志文章
doi：10.1111/j.1471-4159.1985.tb08744.x
更新日期：1985-04-01 00:00:00
abstract：:[3H]Spiroperidol binding to homogenates of rat striatum is saturable and shows either monophasic or biphasic saturation isotherms under specified conditions. In poorly washed membrane fragment preparations, saturation isotherms of [3H]spiroperidol binding are monophasic, revealing an apparently homogeneous set of site...
journal_title：Journal of neurochemistry
pub_type： 杂志文章
doi：10.1111/j.1471-4159.1980.tb07865.x
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journal_title：Journal of neurochemistry
pub_type： 杂志文章
doi：10.1111/j.1471-4159.2004.02528.x
更新日期：2004-08-01 00:00:00
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journal_title：Journal of neurochemistry
pub_type： 杂志文章
doi：10.1111/j.1471-4159.2007.04754.x
更新日期：2007-10-01 00:00:00
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journal_title：Journal of neurochemistry
pub_type： 杂志文章
doi：10.1111/jnc.13888
更新日期：2017-01-01 00:00:00
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journal_title：Journal of neurochemistry
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doi：10.1111/j.1471-4159.2010.07083.x
更新日期：2011-01-01 00:00:00
