Abstract:
:The metabolism of chenodeoxycholic acid by Pseudomonas sp. NCIB 10590 under strict anaerobic conditions was studied. A range of unsaturated acidic and neutral metabolites were isolated and identified. The major acidic product was chola-4,6-dien-3-one-24-oic acid whilst the major neutral product was androsta-4,6-dien-3,17-dione. The major acidic products were 7 alpha-hydroxy-5 beta-cholan-3-oxo-24-oic acid, 3-oxo-4,6-pregnadien-20-carboxylic acid, 7 alpha-hydroxy-3-oxo-1,4-pregnadien-20-carboxylic acid, and 7 alpha-hydroxy-3-oxo-4-pregnen-20-carboxylic acid. The minor neutral products were androsta-1,4,6-trien-3,17-dione, 17 beta-hydroxyandrosta-4,6-dien-3,17-dione, 17 beta-hydroxyandrosta-1,4,6-trien-3-one, 7 alpha-hydroxyandrosta-1,4-dien-3,17-dione, and 7 alpha-hydroxyandrost-4-en-3,17-dione. In contrast to aerobic catabolism of chenodeoxycholic acid by Pseudomonas sp. NCIB 10590 in which 1,4-dienone steroids predominate, the major products described in this study are 4,6-dienone steroids. This is because of the induction of a 7 alpha-dehydroxylase enzyme under anaerobic conditions.
journal_name
J Lipid Resjournal_title
Journal of lipid researchauthors
Owen RW,Hill MJ,Bilton RFsubject
Has Abstractpub_date
1983-09-01 00:00:00pages
1109-18issue
9eissn
0022-2275issn
1539-7262journal_volume
24pub_type
杂志文章abstract::14C-labeled oleic acid and (3)H-labeled monoether in a bile salt solution were perfused through three types of plastic tubing. Large proportions of lipid were lost to the walls of silicone rubber and polyvinyl chloride tubes. The major portion of the lipid lost was recoverable only when chloroform-methanol was perfuse...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1971-09-01 00:00:00
abstract::It was postulated that specialized microdomains of the plasma membrane, consistent with caveolae, might play a role in cholesterol trafficking in intestinal cells. The existence, therefore, of caveolin and the role of detergent-resistant microdomains of the plasma membrane in cholesterol trafficking were investigated ...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1998-10-01 00:00:00
abstract::Macrophage infiltration of white adipose tissue (WAT) is implicated in the metabolic complications of obesity. The precipitating event(s) and function(s) of macrophage infiltration into WAT are unknown. We demonstrate that >90% of all macrophages in WAT of obese mice and humans are localized to dead adipocytes, where ...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M500294-JLR200
更新日期:2005-11-01 00:00:00
abstract::Synthesis of apolipoprotein (apo)E in hepatocytes leads to both secretion and retention in cell surface pools. Inclusion of Brefeldin A to HepG2 cells prompted a rapid decrease of cell surface apoE to about 37% of control values after a 3-h incubation. The t(1/2) for this dynamic pool was estimated to be 15 min. In co...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:2001-09-01 00:00:00
abstract::The action of cycloheximide on some parameters of glucose and lipid metabolism was studied in vitro in epidiymal fat pads from fasted rats. Incubation of fat pads with cycloheximide (1 mug/ml) for 2 hours resulted in a two-fold increase in glucose uptake, glucose oxidation, incorporation of glucose into lipids, and re...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1977-01-01 00:00:00
abstract::The pathogenesis of aortic valve stenosis (AS) is characterized by the accumulation of LDL-derived cholesterol in the diseased valves. Since LDL particles also contain plant sterols, we investigated whether plant sterols accumulate in aortic valve lesions. Serum samples were collected from 82 patients with severe AS a...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M800058-JLR200
更新日期:2008-07-01 00:00:00
abstract::The hydrolysis of HDL phospholipids (PL) and glycerides by hepatic lipase (HL) has been investigated in native and reconstituted HDL particles (Lp2A-I). Fasting, normolipidemic HDL exhibit total lipid hydrolytic rates of between 10 and 36 nM FA/h per microM PL. Of the total fatty acids liberated with HDL3 only 1% are ...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1997-11-01 00:00:00
abstract::Apolipoprotein (apo)E is well established as a secreted protein that plays an important role in systemic lipoprotein metabolism and vascular wall homeostasis. Recently, endogenous expression of apoE in adipocytes has been shown to play an important role in adipocyte lipoprotein metabolism and gene expression consisten...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M011809
更新日期:2011-03-01 00:00:00
abstract::Imidazole catalyzed acylations of lysolipids by acyl-CoAs in water at room temperature and at a pH close to neutrality. In the presence of oleoyl-CoA and either lysophosphatidylcholine, 1-palmitoyl-sn-glycero-3-phosphocholine (LPC); lysophosphatidylglycerol, monoacyl-sn-glycero-3-phosphoglycerol; lysophosphatidyl acid...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.m200075-jlr200
更新日期:2002-07-01 00:00:00
abstract::The effect of various levels of the oral contraceptive drug, Enovid E, on serum and liver lipid levels of adult female rats has been investigated. Doses ranging from 0.052 to 1.04 mg/day have been employed in rats fed control or cholesterol-containing diets. It has been confirmed that after administration of even low,...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1971-05-01 00:00:00
abstract::The Niemann-Pick C1 (NPC1) protein regulates cholesterol transport from late endosomes-lysosomes to other intracellular compartments. In this article, cholesterol transport to caveolin-1 and caveolin-2 containing compartments, such as the trans-Golgi network (TGN) and plasma membrane caveolae, was examined in normal (...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:2002-04-01 00:00:00
abstract::A positive effect of all-trans retinoic acid (ATRA) on white adipose tissue (WAT) oxidative and thermogenic capacity has been described and linked to an in vivo fat-lowering effect of ATRA in mice. However, little is known about the effects of ATRA on mitochondria in white fat. Our objective has been to characterize t...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M053652
更新日期:2015-06-01 00:00:00
abstract::The recognition that the 7 alpha-hydroxylation of 27-hydroxycholesterol is catalyzed by an enzyme that is different from cholesterol 7 alpha-hydroxylase raises the question as to the number of similar enzymes that may be present in liver and subserve bile acid synthesis. Thus, both 3 beta-hydroxy-5-cholestenoic acid a...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1996-06-01 00:00:00
abstract::The percentage of saturated cholesteryl esters (CEs) synthesized by human LCAT is several times higher than expected from the sn-2 acyl composition of plasma phosphatidylcholine (PC), whereas the synthesis of 20:4 CE and 22:6 CE is much lower than expected. To explain these discrepancies, we proposed that LCAT transfe...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M400197-JLR200
更新日期:2004-12-01 00:00:00
abstract::Conjugated linoleic acid (CLA), a mixture of isomers of linoleic acid, has previously been shown to be able to decrease porcine subcutaneous (SC) adipose tissue levels while increasing the count of intramuscular (IM) adipose tissue in vivo. However, the underlying mechanisms through which it acts are poorly understood...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M600525-JLR200
更新日期:2007-08-01 00:00:00
abstract::The total nitrogen and L-hydroxyproline concentrations of two intra-abdominal and two subcutaneous sites of adipose tissue were measured in male and female Wistar rats. At 100 g body weight the nitrogen concentration of intra-abdominal adipose tissue was slightly lower than that of subcutaneous adipose tissue. As age ...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1970-07-01 00:00:00
abstract::This study has examined the dimeric/oligomeric forms of scavenger receptor class B type I (SR-BI) and its alternatively spliced form, SR-BII, in a diverse group of cells and tissues: i.e., normal and hormonally altered tissues of mice and rats as well as tissues of transgenic animals and genetically altered steroidoge...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M300370-JLR200
更新日期:2004-03-01 00:00:00
abstract::Membrane-protein interaction plays key roles in a wide variety of biological processes. Although various methods have been employed to measure membrane binding of soluble proteins, a robust high-throughput assay that is universally applicable to all proteins is lacking at present. Here we report a new fluorescence que...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.D041376
更新日期:2013-12-01 00:00:00
abstract::In eukaryotes, many cell surface proteins are attached to the plasma membrane via a glycolipid glycosylphosphatidylinositol (GPI) anchor. GPI-anchored proteins (GPI-APs) receive the GPI anchor as a conserved posttranslational modification in the lumen of the endoplasmic reticulum (ER). After anchor attachment, the GPI...
journal_title:Journal of lipid research
pub_type: 杂志文章,评审
doi:10.1194/jlr.R062760
更新日期:2016-03-01 00:00:00
abstract::We have performed plasma lipid profiling using liquid chromatography electrospray ionization tandem mass spectrometry on a population cohort of more than 1,000 individuals. From 10 μl of plasma we were able to acquire comparative measures of 312 lipids across 23 lipid classes and subclasses including sphingolipids, ph...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.P035808
更新日期:2013-10-01 00:00:00
abstract::The role of nuclear membrane phospholipids as targets of phospholipases resulting in the generation of nuclear signaling messengers has received attention. In the present study, we have exploited the utility of electrospray ionization mass spectrometry to determine the phospholipid content of nuclei isolated from perf...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:2000-10-01 00:00:00
abstract::Rat spermatogenic cells contain sphingomyelins (SMs) and ceramides (Cers) with very long-chain PUFAs (VLCPUFAs) in nonhydroxylated (n-V) and 2-hydroxylated (h-V) forms. How these atypical species distribute among membrane fractions during differentiation was investigated here using a detergent-free procedure to isolat...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M072595
更新日期:2017-03-01 00:00:00
abstract::Genetic polymorphisms of apolipoprotein A-IV (apoA-IV) have been detected by isoelectric focusing of serum proteins. Because genetic variation in apoA-IV has significant effects on lipid risk factors, we used restriction enzyme isoform genotyping (restriction isotyping) to determine apoA-IV isoform genotypes at the DN...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1991-09-01 00:00:00
abstract::To examine the validity of the deuterium (D) incorporation technique for measurement of human cholesterol synthesis rates, D uptake from D2O into cholesterol was compared to sterol balance in 13 subjects each under three controlled diet settings. Subjects (age 62 +/- 3.6 yr, body weight 74 +/- 4.0 kg, BMI 27 +/- 1.4) ...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1998-05-01 00:00:00
abstract::Mixed background SHP(-/-) mice are resistant to diet-induced obesity due to increased energy expenditure caused by enhanced PGC-1α expression in brown adipocytes. However, congenic SHP(-/-) mice on the C57BL/6 background showed normal expression of PGC-1α and other genes involved in brown adipose tissue thermogenesis....
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M016048
更新日期:2011-12-01 00:00:00
abstract::Several different processes involved in the metabolic fate of docosahexaenoic acid (DHA, C22:6n-3) and its precursor in the biosynthesis route, C24:6n-3, were studied. In cultured skin fibroblasts, the oxidation rate of [1-14C] 24:6n-3 was 2.7 times higher than for [1-14C]22:6n-3, whereas [1-14C]22:6n-3 was incorporat...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.M300223-JLR200
更新日期:2003-10-01 00:00:00
abstract::This paper presents a case of typical hyperlipoproteinemia type I in a young woman. Her serum triglycerides varied between 2 and 90 mmol/l and she had substantial amounts of apolipoprotein B-48 in fasting plasma. She had no detectable lipoprotein lipase (LPL) activity in post-heparin plasma (less than 0.2 percent of n...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1990-07-01 00:00:00
abstract::Apoliprotein (apo) B-100-containing very low density lipoprotein (VLDL) particles secreted from the liver accumulate in plasma during alimentary lipemia. To determine whether changes of VLDL composition occur in the postprandial state that may render these lipoproteins more atherogenic, apoE, C-I, C-II, and C-III, and...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1997-02-01 00:00:00
abstract::The diffusion of [2-(14)C]glucose out of liposomes prepared from extracted human erythrocyte lipids was examined. Increased glucose efflux was observed when the lipids were treated with hydrogen peroxide and CuCl(2) before liposome formation, and this phenomenon required both peroxide and metal. Peroxidation of these ...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1974-05-01 00:00:00
abstract::The beta-oxidation of stearic acid and of alpha- and gamma-methyl isoprenoid-derived fatty acids (pristanic and tetramethylheptadecanoic acids, respectively) was investigated in normal skin fibroblasts and in fibroblasts from patients with inherited defects in peroxisomal biogenesis. Stearic acid beta-oxidation by nor...
journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1990-02-01 00:00:00