Abstract:
:AKR-2B mouse fibroblasts were treated with 50 micrograms/ml of crude transforming growth factor (TGF) of human origin. Cell surface proteins of treated cells were radioiodinated and compared to untreated cells at various times after the addition of TGF. Treated cells showed a severalfold increase (approximately 6-fold) in cell surface 125I incorporation relative to normal cells at 24 h. Electrophoretic comparison of treated and untreated cells showed large increases in the labelling of cell surface proteins of mol. wt. 50,000-90,000 from TGF-exposed cells between 10 and 24 h post treatment. By 48 h post treatment, the electrophoretic profiles of TGF-exposed cells had returned to a pattern similar to that of untreated cells. However, even after a 48 h exposure to TGF, the cells retained a transformed morphology indicating that the electrophoretic alterations were not simply due to the morphological transformation induced by TGF. The electrophoretic pattern of TGF-treated cells at 24 h post treatment was similar to that of AKR-2B cells permanently transformed by treatment with methylcholanthrene, but was clearly distinct from that induced by treatment of normal AKR-2B cells with epidermal growth factor (EGF). EGF induced an increase in a protein of mol. wt. 60,000 in the electrophoretic profiles taken 24 h post treatment. As with TGF, the appearance of electrophoretic profiles of EGF-treated cells returned to "normal" by 48 h. Again, these alterations did not appear to be dependent upon morphological changes since EGF-treated cells showed a morphological transformation similar to that of cells treated with TGF, and this was maintained throughout the 48 h experimental period.
journal_name
Int J Cancerjournal_title
International journal of cancerauthors
Marks ME,Brattain MGdoi
10.1002/ijc.2910330121subject
Has Abstractpub_date
1984-01-15 00:00:00pages
139-46issue
1eissn
0020-7136issn
1097-0215journal_volume
33pub_type
杂志文章abstract::Elevated levels of expression of the urokinase-type plasminogen activator (uPA) and its inhibitor PAI-1 have shown to be related to poor prognosis in a variety of cancer types. In the present study, cytosolic levels of uPA and PAI-1 were determined with enzyme-linked immunosorbent assays in cytosols prepared from 244 ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0215(19961220)69:6<475::AID-IJC
更新日期:1996-12-20 00:00:00
abstract::The Cancer Incidence in Five Continents (CI5) series comprises nine volumes that bring together peer-reviewed results from population-based cancer registries worldwide. The aim of each is to make available comparable data on cancer incidence from as wide a range of geographical locations as possible. In addition, the ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.25517
更新日期:2010-12-15 00:00:00
abstract::The role of the mismatch repair (MMR) system in correcting base-base mismatches is well established; its involvement in the response to DNA double strand breaks, however, is less clear. We investigated the influence of the essential component of MMR, the hMLH1 protein, on the cellular response to DNA-double strand bre...
journal_title:International journal of cancer
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doi:10.1002/ijc.24893
更新日期:2010-06-15 00:00:00
abstract::TP53 is mutated in 20-25% of aggressive B-cell lymphoma (B-NHL). To date, no studies have addressed the impact of TP53 mutations in prospective clinical trial cohorts. To evaluate the impact of TP53 mutation to current risk models in aggressive B-NHL, we investigated TP53 gene mutations within the RICOVER-60 trial. Of...
journal_title:International journal of cancer
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1002/ijc.30838
更新日期:2017-10-01 00:00:00
abstract::Colorectal cancer (CRC) screening rates are currently suboptimal. Blood-based screening could improve rates of earlier detection for CRC and adenomatous colorectal polyps. In this study, we evaluated the feasibility of plasma-based detection of early CRC and adenomatous polyps using array-mediated analysis methylation...
journal_title:International journal of cancer
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doi:10.1002/ijc.26484
更新日期:2012-09-01 00:00:00
abstract::Previous studies have reported an association between hot tea drinking and risk of esophageal cancer, but no study has examined this association using prospectively and objectively measured tea drinking temperature. We examined the association of tea drinking temperature, measured both objectively and subjectively at ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.32220
更新日期:2020-01-01 00:00:00
abstract::Within the Netherlands Cohort Study on diet and cancer, we investigated associations between total alcohol consumption, specific alcoholic beverage consumption and risk of colorectal cancer (CRC) according to anatomical subsite. Hazard Ratios (HR) and 95% confidence intervals (CI) were estimated using Cox proportional...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.23774
更新日期:2008-11-15 00:00:00
abstract::Breast cancer is the leading cancer diagnosis and second most common cause of cancer deaths in sub-Saharan Africa (SSA). Yet, there are few population-level survival data from Africa and none on the survival differences by stage at diagnosis. Here, we estimate breast cancer survival within SSA by area, stage and count...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.32406
更新日期:2020-03-01 00:00:00
abstract::A correlation analysis was performed on 125 cytogenetically characterized breast cancer cases to assess the relationship between the tumor karyotype and clinicopathologic features. The carcinomas of young women had a higher modal chromosome number than those of older women. The number of chromosomal aberrations and mo...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0215(19960410)66:2<191::AID-IJC
更新日期:1996-04-10 00:00:00
abstract::Expression of the monocyte chemoattractant protein-1 (MCP-1) was examined in human central nervous system tumours (glioblastomas and astrocytomas) and normal human brain. Northern blot analysis demonstrated constitutive expression of MCP-1 mRNA in 6 of 12 glioblastoma cell lines. Expression could be stimulated by inte...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910580216
更新日期:1994-07-15 00:00:00
abstract::Seminoma lesions are characterized by a brisk inflammatory infiltrate containing both CD4 and CD8 T cells, which is of prognostic significance. However, whether seminoma cells express the HLA molecules required for classical T-cell recognition remains controversial. In the present study, we conducted a molecular, phen...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.21894
更新日期:2006-08-15 00:00:00
abstract::Tumour cell anaerobic metabolism has been reported to be a prognostic factor in colorectal cancer. The present study investigated the association between monocarboxylate transporter (MCT) 1, MCT 2, lactate dehydrogenase (LDH) 1 and LDH 5, the tumour microenvironment, and outcome in patients with colorectal cancer. A c...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.32045
更新日期:2019-05-01 00:00:00
abstract::Non-small-cell lung cancers (NSCLC) are often infiltrated by T lymphocytes. It is postulated that the presence of tumor-infiltrating lymphocytes (TIL) reflects a local host immune response against autologous tumors. To identify the nature of NSCLC TIL, we have characterized the molecular structure of the TCRbeta chain...
journal_title:International journal of cancer
pub_type: 临床试验,杂志文章
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更新日期:1999-04-12 00:00:00
abstract::In mammals, transforming growth factor-beta (TGF-beta) is found in 3 highly homologous isoforms that exert their effects via heteromeric complexes of type-I and type-II receptors (TbetaR-I and TbetaR-II). TGF-beta regulates the growth and metabolism of various cell types, including keratinocytes. We have investigated ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19970516)71:4<505::aid-ijc
更新日期:1997-05-16 00:00:00
abstract::Alterations of gene expression in squamous cell carcinoma (SCC) cell lines derived from the larynx and keratinocytes derived from adjacent normal mucosa of the larynx have been studied using the mRNA differential display technique. Lane-to-lane comparison of reverse transcribed mRNA showed a strong repression of a 148...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19991210)83:6<750::aid-ijc
更新日期:1999-12-10 00:00:00
abstract::Ocular adnexal MALT lymphoma (OAML) is linked to Chlamydophila psittaci (Cp) infection. Viability and infectivity of Cp, demonstrated by growth in culture, has not been yet investigated in these patients. We conducted a single-center prospective case-control study to assess the prevalence, viability and infectivity of...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.23596
更新日期:2008-09-01 00:00:00
abstract::The human surface antigen CD24 is over-expressed in small-cell lung cancer. Here we describe the isolation, sequencing and functional characterization of the 5'-flanking region of the human CD24 gene. A sequence (accession number: Y14692) of 3.4 kb regulates the activity of a luciferase reporter gene in CD24-expressin...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19981109)78:4<496::aid-ijc
更新日期:1998-11-09 00:00:00
abstract::Administration of highly purified interferon to DBA/2 mice inhibited the growth of interferon-sensitive or interferon-resistant Friend erythroleukemia cells implanted subcutaneously. Injection of interferon at the site of tumor inoculation was more effective than injection of interferon intraperitoneally. Histologic e...
journal_title:International journal of cancer
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doi:10.1002/ijc.2910310518
更新日期:1983-05-15 00:00:00
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journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910180615
更新日期:1976-12-15 00:00:00
abstract::We studied if bladder cancers respond to HAMLET (human alpha-lactalbumin made lethal to tumor cells) to establish if intravesical HAMLET application might be used to selectively remove cancer cells in vivo. Patients with nonmuscle invasive transitional cell carcinomas were included. Nine patients received 5 daily intr...
journal_title:International journal of cancer
pub_type: 临床试验,杂志文章
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更新日期:2007-09-15 00:00:00
abstract::Thrombin is now recognized as an important factor in many cancers. Here, we examined the expression and role of the recently discovered thrombin receptor PAR4, in human colon cancer cells. PAR4 mRNA was found in 10 out of 14 (71%) human colon cancer cell lines tested but not in epithelial cells isolated from normal hu...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.24070
更新日期:2009-04-01 00:00:00
abstract::Malignant lymphomas frequently develop in the pleural cavity of patients with long-standing pyothorax. The term pyothorax-associated lymphoma (PAL) has been proposed for this type of tumor. Most PALs are diffuse lymphomas of the B-cell type and contain Epstein-Barr virus (EBV) DNA. We have established 2 lymphoma cell ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0215(19960703)67:1<86::AID-IJC1
更新日期:1996-07-03 00:00:00
abstract::Human non-small cell lung cancer (NSCLC) cells were transfected with recombinant prodrug herpes simplex virus type I thymidine kinase (HSV-tk) cDNA, and the selected clones underwent apoptosis in response to induction by antiviral ganciclovir (GCV). The efficiency of GCV-induced growth inhibition and the extent of the...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.10701
更新日期:2002-12-01 00:00:00
abstract::Osteosarcomas are primary bone tumors of osteoblastic origin that mostly affect adolescent patients. These tumors are highly aggressive and metastatic. Previous reports indicate that gain of function of a key osteoblastic differentiation factor, Runx2, leads to growth inhibition in osteosarcoma. We have previously est...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.25024
更新日期:2010-07-01 00:00:00
abstract::To evaluate renal pelvis and ureter (RPU) cancer risk in relation to lifetime use of analgesics, a population-based case-control study was carried out in 3 areas of the United States. Among 502 cases and 496 controls diagnosed and interviewed during 1983-1986, no significant increases in risk were found for any of the...
journal_title:International journal of cancer
pub_type: 杂志文章,多中心研究
doi:10.1002/ijc.2910620105
更新日期:1995-07-04 00:00:00
abstract::Artemisia asiatica Nakai has been used in traditional Asian medicine for the treatment of inflammatory and other disorders. Previous studies have revealed that the formulated ethanol extract (DA-9601) of A. asiatica has pronounced antioxidative and antiinflammatory activities and exhibits cytoprotective effects agains...
journal_title:International journal of cancer
pub_type: 杂志文章
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更新日期:2002-08-01 00:00:00
abstract::The aim of this study was to construct bispecific F(ab')2 [anti-CD3 x anti-BCL1 idiotype (Id)] Abs (BsAbs) which would enable lymphokine-activated killer (LAK) T cells to kill Id+ mouse BCL1 lymphoma cells, and to determine the mechanism(s) underlying cell death. Using 4-day activated LAK T cells from either perforin-...
journal_title:International journal of cancer
pub_type: 杂志文章
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更新日期:1996-12-11 00:00:00
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journal_title:International journal of cancer
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更新日期:2020-03-15 00:00:00
abstract::With the aim of identifying innovative therapeutic strategies for osteosarcoma patients who are refractory to conventional chemotherapy, we analyzed the in vitro effects of the blockage of autocrine circuits. Since the insulin-like growth factor-I receptor (IGF-IR)-mediated loop is relevant to the growth of osteosarco...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19990209)80:4<581::aid-ijc
更新日期:1999-02-09 00:00:00
abstract::The reported beverage habits of 569 lung cancer patients and 569 control patients admitted to Roswell Park Memorial Institute (RPMI) were studied, 355 male cases and 214 female cases being matched to controls within strata of age and residence. Smoking history and an index of vitamin A from vegetables had significant,...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910430412
更新日期:1989-04-15 00:00:00