Synthesis of molecular species of glycerophospholipids from diglyceride-labeled brain microsomes.

abstract：:The accumulation of phosphatidylcholine hydroperoxide (PCOOH), a primary oxidation product of phosphatidylcholine (PC), in blood plasma and tissues has been observed in various pathological conditions, including atherosclerosis. However, the biological roles of PCOOH in these conditions remain unknown. To estimate the...

journal_title：Journal of lipid research

authors： Asai A,Okajima F,Nakagawa K,Ibusuki D,Tanimura K,Nakajima Y,Nagao M,Sudo M,Harada T,Miyazawa T,Oikawa S

更新日期：2009-05-01 00:00:00

abstract：:Human endothelial lipase (EL), a member of the triglyceride lipase gene family, has five potential N-linked glycosylation sites, two of which are conserved in both lipoprotein lipase and hepatic lipase. Reduction in molecular mass of EL after treatment with glycosidases and after treatment of EL-expressing cells with ...

journal_title：Journal of lipid research

authors： Miller GC,Long CJ,Bojilova ED,Marchadier D,Badellino KO,Blanchard N,Fuki IV,Glick JM,Rader DJ

更新日期：2004-11-01 00:00:00

abstract：:The selective and reversible adsorption of bovine low density lipoproteins (LDL) by heparin-Sepharose has been exploited as the critical step in a procedure for the preparative isolation of very low density lipoproteins (VLDL)/chylomicrons, LDL, and high density lipoproteins (HDL) from bovine plasma. Molecular size ex...

journal_title：Journal of lipid research

authors： Cordle SR,Clegg RA,Yeaman SJ

更新日期：1985-06-01 00:00:00

abstract：:The density distribution of electronegative LDL [LDL(-)], a cytotoxic and inflammatory fraction of LDL present in plasma, was studied in 10 normolipemic (NL), 6 FH, and 11 hypertriglyceridemic (HTG) subjects. Six LDL subclasses of increased density (LDL1 to LDL6) were isolated by density-gradient ultracentrifugation (...

journal_title：Journal of lipid research

authors： Sánchez-Quesada JL,Benítez S,Otal C,Franco M,Blanco-Vaca F,Ordóñez-Llanos J

更新日期：2002-05-01 00:00:00

abstract：:The formation of hepatic bile requires that water be transported across liver epithelia. Rat hepatocytes express three aquaporins (AQPs): AQP8, AQP9, and AQP0. Recognizing that cholesterol and sphingolipids are thought to promote the assembly of proteins into specialized membrane microdomains, we hypothesized that can...

journal_title：Journal of lipid research

authors： Tietz P,Jefferson J,Pagano R,Larusso NF

更新日期：2005-07-01 00:00:00

abstract：:Matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS) has been applied primarily to the analysis of glycosphingolipids separated from other complex mixtures by TLC, but it is difficult to obtain quantitative profiling of each glycosphingolipid among the different spots on TLC by MALDI-MS. Thus, the ...

journal_title：Journal of lipid research

authors： Ikeda K,Shimizu T,Taguchi R

更新日期：2008-12-01 00:00:00

abstract：:The ionization behavior of cholic acid, deoxycholic acid, and chenodeoxycholic acid in a variety of physiologically important molecular environments was studied using 13C NMR spectroscopy. The apparent pKa of the carboxyl group was determined from titration curves obtained from the dependence of the carboxyl carbon ch...

journal_title：Journal of lipid research

authors： Cabral DJ,Hamilton JA,Small DM

更新日期：1986-03-01 00:00:00

abstract：:Wax esters are neutral lipids exhibiting desirable properties for lubrication. Natural sources have traditionally been whales. Additionally some plants produce wax esters in their seed oil. Currently there is no biological source available for long chain length monounsaturated wax esters that are most suited for indus...

journal_title：Journal of lipid research

authors： Heilmann M,Iven T,Ahmann K,Hornung E,Stymne S,Feussner I

更新日期：2012-10-01 00:00:00

abstract：:The interaction of hepatic lipase (HL) with heparan sulfate is critical to the function of this enzyme. The primary amino acid sequence of HL was compared to that of lipoprotein lipase (LPL), a related enzyme that possesses several putative heparin-binding domains. Of the three putative heparin-binding clusters of LPL...

journal_title：Journal of lipid research

authors： Sendak RA,Berryman DE,Gellman G,Melford K,Bensadoun A

更新日期：2000-02-01 00:00:00

abstract：:Niacin, the first antidyslipidemic drug, has been at the center stage of lipid research for many decades before the discovery of statins. However, to date, despite its remarkable effects on lipid profiles, the clinical outcomes of niacin treatment on cardiac events is still debated. In addition to its historically wel...

journal_title：Journal of lipid research

authors： Romani M,Hofer DC,Katsyuba E,Auwerx J

更新日期：2019-04-01 00:00:00

abstract：:Human apolipoprotein C-I (apoC-I) is an exchangeable apolipoprotein that binds to lipoprotein particles in vivo. In this study, we employed a LC-MS/MS assay to demonstrate that residues 38-51 of apoC-I are significantly protected from proteolysis in the presence of 1,2-dimyristoyl-3-sn-glycero-phosphocholine (DMPC). T...

journal_title：Journal of lipid research

authors： James PF,Dogovski C,Dobson RC,Bailey MF,Goldie KN,Karas JA,Scanlon DB,O'Hair RA,Perugini MA

更新日期：2009-07-01 00:00:00

abstract：:Animal experiments show that the kidney contributes to apolipoprotein (apo)A-I catabolism. We tested relationships of HDL cholesterol (HDL-C) and apo-I with kidney function in subjects without severe chronic kidney disease. Included was a random sample of the general population (part of the PREVEND cohort). Kidney fun...

journal_title：Journal of lipid research

authors： Krikken JA,Gansevoort RT,Dullaart RP,PREVEND Study Group.

更新日期：2010-07-01 00:00:00

abstract：:Distinct isoforms of long-chain acyl-CoA synthetases (ACSLs) may partition fatty acids toward specific metabolic cellular pathways. For each of the five members of the rat ACSL family, we analyzed tissue mRNA distributions, and we correlated the mRNA, protein, and activity of ACSL1 and ACSL4 after fasting and refeedin...

journal_title：Journal of lipid research

authors： Mashek DG,Li LO,Coleman RA

更新日期：2006-09-01 00:00:00

abstract：:Lipid transfer inhibitor protein (LTIP) is a regulator of cholesteryl ester transfer protein (CETP) function. Factors affecting plasma LTIP levels are poorly understood. In humans, plasma LTIP is elevated in hypercholesterolemia. To define possible mechanisms by which hyperlipidemia modifies LTIP, we investigated the ...

journal_title：Journal of lipid research

authors： Izem L,Morton RE

更新日期：2009-04-01 00:00:00

abstract：:In breast cancer, 17β-estradiol (E2) plays critical roles mainly by binding to its canonical receptor, estrogen receptor (ER) α66, and eliciting genomic effects. E2 also triggers rapid, nongenomic responses. E2 activates sphingosine kinase 1 (SphK1), increasing sphingosine-1-phosphate (S1P) that binds to its receptors...

journal_title：Journal of lipid research

authors： Maczis MA,Maceyka M,Waters MR,Newton J,Singh M,Rigsby MF,Turner TH,Alzubi MA,Harrell JC,Milstien S,Spiegel S

更新日期：2018-12-01 00:00:00

abstract：:The skin surface represents our interface with the external environment, and as such, is our first line of defense against microbial colonization and infection. Lipids at the skin surface are thought to underlie at least part of an antimicrobial barrier. Some of these lipids are synthesized in the epidermis and are ca...

journal_title：Journal of lipid research

authors： Drake DR,Brogden KA,Dawson DV,Wertz PW

更新日期：2008-01-01 00:00:00

abstract：:We determined the effect of storage at -70 degrees C on the determination of plasma high density lipoprotein (HDL)-cholesterol. Plasma from 106 subjects was stored for 1, 6, and 12 months, then treated with heparin and MnCl2 to remove other lipoproteins, and HDL-cholesterol was measured. The mean HDL-cholesterol level...

journal_title：Journal of lipid research

authors： Bachorik PS,Walker RE,Kwiterovich PO Jr

更新日期：1982-11-01 00:00:00

abstract：:Eubacterium sp. 144 dehydroxylated 16 alpha-hydroxy-progesterone; however, the expected intermediate, delta 16-progesterone, did not accumulate to significant concentrations in the culture medium. Moreover, the final end product of this biotransformation, 17 alpha-progesterone, was produced at a very slow rate. It was...

journal_title：Journal of lipid research

authors： Glass TL,Winter J,Bokkenheuser VD,Hylemon PB

更新日期：1982-02-01 00:00:00

abstract：:A-C1 protein is the product of a tumor suppressor gene negatively regulating the oncogene Ras and belongs to the HRASLS (HRAS-like suppressor) subfamily. We recently found that four members of this subfamily expressed in human tissues function as phospholipid-metabolizing enzymes. Here we examined a possible enzyme ac...

journal_title：Journal of lipid research

authors： Shinohara N,Uyama T,Jin XH,Tsuboi K,Tonai T,Houchi H,Ueda N

更新日期：2011-11-01 00:00:00

abstract：:Lipid-containing deposits within the swim bladders of Coryphaenoides acrolepis and Antimora rostrata were investigated. Lipid analysis of this material, which was quite uniform from the two species, yielded the following data: neutral lipids, 36.0-41.7%; phospholipids, 53.6-56.7%; and glycolipids, 4.3-8.9%. Cholestero...

journal_title：Journal of lipid research

authors： Patton S,Thomas AJ

更新日期：1971-05-01 00:00:00

abstract：:Mature endothelial lipase (EL) is a 68 kDa glycoprotein. In HepG2 cells infected with adenovirus encoding human EL, the mature EL was detectable in the cell lysates and heparin-releasable fractions. In contrast, cell media of these cells contained two EL fragments: an N-terminal 40 kDa fragment and a C-terminal 28 kDa...

journal_title：Journal of lipid research

authors： Gauster M,Hrzenjak A,Schick K,Frank S

更新日期：2005-05-01 00:00:00

abstract：:Mutations in the genes coding for patatin-like phospholipase domain-containing 1 (PNPLA1) and α/β-hydrolase domain-containing 5 (ABHD5), also known as comparative gene identification 58, are causative for ichthyosis, a severe skin barrier disorder. Individuals with mutations in either of these genes show a defect in e...

journal_title：Journal of lipid research

authors： Kien B,Grond S,Haemmerle G,Lass A,Eichmann TO,Radner FPW

更新日期：2018-12-01 00:00:00

abstract：:Ceramides play an essential role in modulating immune signaling pathways and proinflammatory cytokine production in response to infectious pathogens, stress stimuli, or chemotherapeutic drugs. In this study, we demonstrated that Aggregatibacter actinomycetemcomitans, the pathogen for aggressive periodontitis, induced ...

journal_title：Journal of lipid research

authors： Yu H,Valerio M,Bielawski J

更新日期：2013-01-01 00:00:00

abstract：:Lymph fistula rats with either biliary fistula or pancreaticobiliary fistula were used to measure coenzyme A-independent mucosal cholesterol esterifying activity in the presence and absence of pancreatic exocrine secretion. Efficiency of pancreatic diversion was verified to minimize contamination of mucosal homogenate...

journal_title：Journal of lipid research

authors： Watt SM,Simmonds WJ

更新日期：1981-01-01 00:00:00

abstract：:Binding of the medium-chain fatty acids (MCFA), octanoic (OCT) and decanoic (DEC) acid, to human serum albumin (HSA) has been studied by 13C NMR spectroscopy. NMR spectra at 35 degrees C showed an apparently homogeneous binding environment (a single, narrow resonance for the 13C-enriched carboxyl carbon) at different ...

journal_title：Journal of lipid research

authors： Kenyon MA,Hamilton JA

更新日期：1994-03-01 00:00:00

abstract：:The absorption and distribution of deuterated trans- and cis-13-octadecenoic acid (13t-18:1 and 13c-18:1) in plasma lipids were compared to deuterated cis-9-octadecenoic acid (9c-18:1) in two young adult male subjects. A mixture of triglycerides was fed in a multiple-labeled experiment where each triglyceride containe...

journal_title：Journal of lipid research

authors： Emken EA,Adlof RO,Rohwedder WK,Gulley RM

更新日期：1983-01-01 00:00:00

abstract：:Rat liver microsomes were incubated with the monofunctional aminoreagent fluorescamine. Although the probe easily penetrated the membranes, two pools of phosphatidylethanolamine (PE) could be detected. The first pool rapidly reacted with the probe and comprised 80% of the total PE. The second pool exhibited a very slo...

journal_title：Journal of lipid research

authors： Valtersson C,Filipsson L,Dallner G

更新日期：1986-07-01 00:00:00

abstract：:The hydrolysis of HDL phospholipids (PL) and glycerides by hepatic lipase (HL) has been investigated in native and reconstituted HDL particles (Lp2A-I). Fasting, normolipidemic HDL exhibit total lipid hydrolytic rates of between 10 and 36 nM FA/h per microM PL. Of the total fatty acids liberated with HDL3 only 1% are ...

journal_title：Journal of lipid research

authors： Coffill CR,Ramsamy TA,Hutt DM,Schultz JR,Sparks DL

更新日期：1997-11-01 00:00:00

abstract：:Cholesteryl ester storage disease (CESD) and Wolman disease (WD) are both autosomal recessive disorders associated with reduced activity and genetic defects of lysosomal acid lipase (LAL). The strikingly more severe course of WD is caused by genetic defects of LAL that leave no residual enzymatic activity. Mutations a...

journal_title：Journal of lipid research

authors： Ries S,Aslanidis C,Fehringer P,Carel JC,Gendrel D,Schmitz G

更新日期：1996-08-01 00:00:00

abstract：:The hepatic uptake of intravenously injected beta-very low density lipoprotein (beta-VLDL) in rabbits fed 2% (w/w) cholesterol for 3 weeks was investigated. In vitro studies were also conducted to examine the specificity and the capacity of the uptake in isolated liver parenchymal cells. The hepatic uptake of beta-VLD...

journal_title：Journal of lipid research

authors： Gudmundsen O,Berg T,Roos N,Nenseter MS

更新日期：1993-04-01 00:00:00