Targeted analysis of ganglioside and sulfatide molecular species by LC/ESI-MS/MS with theoretically expanded multiple reaction monitoring.

abstract：:An enzyme (phosphatidyl glycerophosphate phosphatase) that catalyzes the formation of phosphatidyl glycerol from phosphatidyl glycerophosphate has been rendered soluble by treatment of the particulate fraction of E. coli with Triton X-100 in the presence of EDTA, and has been partially purified. The enzyme is specific...

journal_title：Journal of lipid research

authors： Chang YY,Kennedy EP

更新日期：1967-09-01 00:00:00

abstract：:The rate of lipolytic enzyme-catalyzed reactions yielding water-soluble products can be measured by isobaric titration. The method is based upon the measurement of the amount of substrate that must be added to a monalayer to maintain constant surface pressure during the course of the enzymatic reaction. The rate const...

journal_title：Journal of lipid research

authors： Brockman HL,Kézdy FJ,Law JH

更新日期：1975-01-01 00:00:00

abstract：:Four distinct subspecies of the insect hemolymph lipoprotein, lipophorin, that range in diacylglycerol (DAG) content from approximately 100 to 1000 molecules per particle, were treated with phospholipase C. Lipid analysis demonstrated that both phosphatidylcholine and phosphatidylethanolamine were hydrolyzed to DAG. P...

journal_title：Journal of lipid research

authors： Singh TK,Liu H,Bradley R,Scraba DG,Ryan RO

更新日期：1994-09-01 00:00:00

abstract：:A-C1 protein is the product of a tumor suppressor gene negatively regulating the oncogene Ras and belongs to the HRASLS (HRAS-like suppressor) subfamily. We recently found that four members of this subfamily expressed in human tissues function as phospholipid-metabolizing enzymes. Here we examined a possible enzyme ac...

journal_title：Journal of lipid research

authors： Shinohara N,Uyama T,Jin XH,Tsuboi K,Tonai T,Houchi H,Ueda N

更新日期：2011-11-01 00:00:00

abstract：:In the chick, the large cholesteryl ester (CE) store present in the liver during the last period of embryonic life increases at hatching and is rapidly depleted after 2-7 days of postnatal life. In this study we asked whether these changes were associated with variations in the hepatic production of apoB-containing li...

journal_title：Journal of lipid research

authors： Tarugi P,Nicolini S,Marchi L,Ballarini G,Calandra S

更新日期：1994-11-01 00:00:00

abstract：:Niemann-Pick disease, type C (NP-C), often associated with Niemann-Pick disease, type C1 (NPC1) mutations, is a cholesterol-storage disorder characterized by cellular lipid accumulation, neurodegeneration, and reduced steroid production. To study NPC1 function in vivo, we cloned zebrafish npc1 and analyzed its gene ex...

journal_title：Journal of lipid research

authors： Schwend T,Loucks EJ,Snyder D,Ahlgren SC

更新日期：2011-07-01 00:00:00

abstract：:Our objective was to establish the role of the apoprotein (apo) E phenotype in determining serum cholesterol levels in infants fed exclusively on high-fat, high-cholesterol human milk and in those fed a low-cholesterol, high-unsaturated fat formula. The total and lipoprotein cholesterol, apoB, and triglyceride concent...

journal_title：Journal of lipid research

authors： Kallio MJ,Salmenperä L,Siimes MA,Perheentupa J,Gylling H,Miettinen TA

更新日期：1997-04-01 00:00:00

abstract：:Cholesterol exists within the hepatocyte as free cholesterol and cholesteryl ester. The proportion of intrahepatic cholesterol in the free or ester forms is governed in part by the rate of cholesteryl ester formation by acyl-coenzyme A:cholesterol acyltransferase (ACAT) and cholesteryl ester hydrolysis by neutral chol...

journal_title：Journal of lipid research

authors： Stone BG,Evans CD,Fadden RJ,Schreiber D

更新日期：1989-11-01 00:00:00

abstract：:The reversibility and regression of histological and biochemical findings in a mouse model of Gaucher disease (4L/PS-NA) was evaluated using a liver-enriched activator protein promoter control of a tetracycline-controlled transcriptional activation-responsive human acid beta-glucosidase (hGCase) transgenic system. 4L/...

journal_title：Journal of lipid research

authors： Sun Y,Quinn B,Xu YH,Leonova T,Witte DP,Grabowski GA

更新日期：2006-10-01 00:00:00

abstract：:Biliary lipids (bile salts, phospholipids, cholesterol, plant sterols) were determined in 89 vertebrate species (cartilaginous and bony fish, reptiles, birds, and mammals), and individual phospholipid classes were measured in 35 species. All samples contained conjugated bile salts (C(27) bile alcohol sulfates and/or N...

journal_title：Journal of lipid research

authors： Moschetta A,Xu F,Hagey LR,van Berge-Henegouwen GP,van Erpecum KJ,Brouwers JF,Cohen JC,Bierman M,Hobbs HH,Steinbach JH,Hofmann AF

更新日期：2005-10-01 00:00:00

abstract：:Cholesterol 7alpha-hydroxylase, a rate-limiting enzyme for bile acid synthesis, has been implicated in genetic susceptibility to atherosclerosis. The gene, CYP7A1, encoding a protein with this activity, is expressed normally only in hepatocytes and is highly regulated. Our cyp7A1 gene knockout mouse colony, as young a...

journal_title：Journal of lipid research

authors： Erickson SK,Lear SR,Deane S,Dubrac S,Huling SL,Nguyen L,Bollineni JS,Shefer S,Hyogo H,Cohen DE,Shneider B,Sehayek E,Ananthanarayanan M,Balasubramaniyan N,Suchy FJ,Batta AK,Salen G

更新日期：2003-05-01 00:00:00

abstract：:Oligosaccharyl phosphates (OSPs) are hydrolyzed from oligosaccharide-diphosphodolichol (DLO) during protein N-glycosylation by an uncharacterized process. An OSP-generating activity has been reported in vitro, and here we asked if its biochemical characteristics are compatible with a role in endoplasmic reticulum (ER)...

journal_title：Journal of lipid research

authors： Massarweh A,Bosco M,Iatmanen-Harbi S,Tessier C,Auberger N,Busca P,Chantret I,Gravier-Pelletier C,Moore SE

更新日期：2016-06-01 00:00:00

abstract：:The fatty acid transport protein (FATP) Fat1p in the yeast Saccharomyces cerevisiae functions in concert with acyl-coenzyme A synthetase (ACSL; either Faa1p or Faa4p) in vectorial acylation, which couples the transport of exogenous fatty acids with activation to CoA thioesters. To further define the role of Fat1p in t...

journal_title：Journal of lipid research

authors： Obermeyer T,Fraisl P,DiRusso CC,Black PN

更新日期：2007-11-01 00:00:00

abstract：:Serum amyloid A (SAA) is an acute phase reactant that can become the predominant apolipoprotein of high density lipoprotein (HDL) during severe inflammatory states. However, the function of SAA is unknown. To study the ability of SAA to form HDL in the absence of apolipoprotein A-I, we expressed the mouse SAA pI 6.15 ...

journal_title：Journal of lipid research

authors： Webb NR,de Beer MC,van der Westhuyzen DR,Kindy MS,Banka CL,Tsukamoto K,Rader DL,de Beer FC

更新日期：1997-08-01 00:00:00

abstract：:Acyl-CoA thioesterase (Acot)2 localizes to the mitochondrial matrix and hydrolyses long-chain fatty acyl-CoA into free FA and CoASH. Acot2 is expressed in highly oxi-dative tissues and is poised to modulate mitochondrial FA oxidation (FAO), yet its biological role is unknown. Using a model of adenoviral Acot2 overexpr...

journal_title：Journal of lipid research

authors： Moffat C,Bhatia L,Nguyen T,Lynch P,Wang M,Wang D,Ilkayeva OR,Han X,Hirschey MD,Claypool SM,Seifert EL

更新日期：2014-12-01 00:00:00

abstract：:HDL plays crucial roles at multiple stages of the pathogenesis of atherosclerosis. AMP-activated protein kinase (AMPK) is a therapeutic candidate for the treatment of cardiovascular disease. However, the effect of AMPK activation on HDL functionality has not been established in vivo. We assessed the effects of pharmac...

journal_title：Journal of lipid research

authors： Ma A,Wang J,Yang L,An Y,Zhu H

更新日期：2017-08-01 00:00:00

abstract：:The expansion of lipid droplets (LDs) and the differentiation of preadipocytes are two important aspects of mammalian lipid storage. In this study, we examined the role of CDP-diacylglycerol (DAG) synthases (CDSs), encoded by CDS1 and CDS2 genes in mammals, in lipid storage. CDS enzymes catalyze the formation of CDP-D...

journal_title：Journal of lipid research

authors： Qi Y,Kapterian TS,Du X,Ma Q,Fei W,Zhang Y,Huang X,Dawes IW,Yang H

更新日期：2016-05-01 00:00:00

abstract：:Two novel mutations were identified in a compound heterozygous male with lecithin:cholesterol acyltransferase (LCAT) deficiency. Exon sequence determination of the LCAT gene of the proband revealed two novel heterozygous mutations in exons one (C110T) and six (C991T) that predict non-conservative amino acid substituti...

journal_title：Journal of lipid research

authors： Argyropoulos G,Jenkins A,Klein RL,Lyons T,Wagenhorst B,St Armand J,Marcovina SM,Albers JJ,Pritchard PH,Garvey WT

更新日期：1998-09-01 00:00:00

abstract：:In vitro studies show that docosahexaenoic acid (DHA) can be released from membrane phospholipid by Ca(2+)-independent phospholipase A(2) (iPLA(2)), Ca(2+)-independent plasmalogen PLA(2) or secretory PLA(2 (sPLA2)), but not by Ca(2+)-dependent cytosolic PLA(2) (cPLA2), which selectively releases arachidonic acid (AA)....

journal_title：Journal of lipid research

authors： Ramadan E,Rosa AO,Chang L,Chen M,Rapoport SI,Basselin M

更新日期：2010-08-01 00:00:00

abstract：:We have performed plasma lipid profiling using liquid chromatography electrospray ionization tandem mass spectrometry on a population cohort of more than 1,000 individuals. From 10 μl of plasma we were able to acquire comparative measures of 312 lipids across 23 lipid classes and subclasses including sphingolipids, ph...

journal_title：Journal of lipid research

authors： Weir JM,Wong G,Barlow CK,Greeve MA,Kowalczyk A,Almasy L,Comuzzie AG,Mahaney MC,Jowett JB,Shaw J,Curran JE,Blangero J,Meikle PJ

更新日期：2013-10-01 00:00:00

abstract：:LPL is an enzyme involved in the breakdown and uptake of lipoprotein triglycerides. In the present study, we examined how the transgenic (Tg) overexpression of human LPL in mouse skeletal muscle affected tolerance to cold temperatures, cold-induced thermogenesis, and fuel utilization during this response. Tg mice and ...

journal_title：Journal of lipid research

authors： Jensen DR,Knaub LA,Konhilas JP,Leinwand LA,MacLean PS,Eckel RH

更新日期：2008-04-01 00:00:00

abstract：:Leukotrienes (LTs) play major roles in lung immune responses, and LTD4 is the most potent agonist for cysteinyl LT1, leading to bronchoconstriction and tissue remodeling. Here, we studied LT crosstalk between myeloid cells and pulmonary epithelial cells. Monocytic cells (Mono Mac 6 cell line, primary dendritic cells) ...

journal_title：Journal of lipid research

authors： Lukic A,Ji J,Idborg H,Samuelsson B,Palmberg L,Gabrielsson S,Rådmark O

更新日期：2016-09-01 00:00:00

abstract：:G protein-coupled receptor (GPR)120/FFA receptor (FFAR)4 (GPR120/FFAR4) activation by n-3 PUFAs attenuates inflammation, but its impact on atherosclerosis is unknown. We determined whether in vivo activation of leukocyte GPR120/FFAR4 by n-3 versus n-6 PUFAs is atheroprotective. Leukocyte GPR120/FFAR4 WT or KO mice in ...

journal_title：Journal of lipid research

authors： Shewale SV,Brown AL,Bi X,Boudyguina E,Sawyer JK,Alexander-Miller MA,Parks JS

更新日期：2017-01-01 00:00:00

abstract：:Polyunsaturated fatty acids, including docosahexaenoic acid (DHA, 22:6n-3), modulate immune responses and exert beneficial immunosuppressive effects, but the molecular mechanisms inhibiting T-cell activation are not yet elucidated. Lipid rafts have been shown to play an important role in the compartmentalization and m...

journal_title：Journal of lipid research

authors： Li Q,Wang M,Tan L,Wang C,Ma J,Li N,Li Y,Xu G,Li J

更新日期：2005-09-01 00:00:00

abstract：:The LDL receptor-related protein-1 (LRP1) is highly expressed in numerous cell types, and its impairment is associated with obesity, diabetes, and fatty liver disease. However, the mechanisms linking LRP1 to metabolic disease are not completely understood. Here, we compared the metabolic phenotype of C57BL/6J wild typ...

journal_title：Journal of lipid research

authors： Jaeschke A,Haller A,Cash JG,Nam C,Igel E,Roebroek AJM,Hui DY

更新日期：2020-12-09 00:00:00

abstract：:Ac-hE18A-NH2 is a dual-domain apoE mimetic peptide that possesses the putative receptor binding domain from apoE (LRKLRKRLLR, denoted hE; residues 141-150) covalently attached to lipid-associating peptide 18A. Like apoE, Ac-hE18A-NH2 reduces plasma cholesterol in animal models and exhibits anti-inflammatory properties...

journal_title：Journal of lipid research

authors： Anantharamaiah GM,Garber DW,Goldberg D,Morrel E,Datta G,Palgunachari MN,Register TC,Appt SE,White CR

更新日期：2018-11-01 00:00:00

abstract：:Lipid transfer inhibitor protein (LTIP) is a regulator of cholesteryl ester transfer protein (CETP) function. Factors affecting plasma LTIP levels are poorly understood. In humans, plasma LTIP is elevated in hypercholesterolemia. To define possible mechanisms by which hyperlipidemia modifies LTIP, we investigated the ...

journal_title：Journal of lipid research

authors： Izem L,Morton RE

更新日期：2009-04-01 00:00:00

abstract：:Circulating triacylglycerol (TG) arises mainly from dietary fat. However, little is known about the entry of dietary fat into the major TG pool, very low-density lipoprotein (VLDL) TG. We used a novel method to study the specific incorporation of dietary fatty acids into postprandial VLDL TG in humans. Eight healthy v...

journal_title：Journal of lipid research

authors： Heath RB,Karpe F,Milne RW,Burdge GC,Wootton SA,Frayn KN

更新日期：2003-11-01 00:00:00

abstract：:To assess the relationship between cholesterol synthesis and feedback inhibition of bile acid synthesis, we studied seven normal human subjects taking three different doses of chenodiol, 0, 5, and 15 mg/kg per day: once while taking no lovastatin and again while taking lovastatin 80 mg/day. Lovastatin and both doses o...

journal_title：Journal of lipid research

authors： Hanson DS,Duane WC

更新日期：1994-08-01 00:00:00

abstract：:Whereas antibipolar drug administration to rats reduces brain arachidonic acid turnover, excessive N-methyl-d-aspartate (NMDA) signaling is thought to contribute to bipolar disorder symptoms and may increase arachidonic acid turnover in rat brain phospholipids. To determine whether chronic NMDA would increase brain ar...

journal_title：Journal of lipid research

authors： Lee HJ,Rao JS,Chang L,Rapoport SI,Bazinet RP

更新日期：2008-01-01 00:00:00