Density distribution of electronegative LDL in normolipemic and hyperlipemic subjects.

Abstract:

:The density distribution of electronegative LDL [LDL(-)], a cytotoxic and inflammatory fraction of LDL present in plasma, was studied in 10 normolipemic (NL), 6 FH, and 11 hypertriglyceridemic (HTG) subjects. Six LDL subclasses of increased density (LDL1 to LDL6) were isolated by density-gradient ultracentrifugation (DGU). NL and FH subjects showed prevalence of light LDL, whereas HTG subjects showed prevalence of dense LDL. LDL(-) proportion was determined from total LDL or LDL-density subclasses by anion-exchange chromatography. LDL from FH patients had increased LDL(-) (35.1 +/- 9.9%) compared with LDL from NL and HTG subjects (9.4 +/- 2.3% and 12.3 +/- 4.3%, respectively). Most LDL(-) was contained in dense subclasses in NL (LDL4-6, 67.7 +/- 3.1%) whereas most of LDL(-) from FH patients were contained in light LDL subclasses (LDL1-3) (86.2 +/- 1.6%). In these subjects, simvastatin therapy decreased LDL(-) to 28.2 +/- 6.7% and 21.2 +/- 5.6% at 3 and 6 months of treatment, respectively, due mainly to decreases in light LDL subclasses. In HTG subjects, half LDL(-) was contained in dense LDL subclasses (LDL4-6, 46.1 +/- 2.0%). Non-denaturing acrylamide gradient gel electrophoresis concurred with DGU data, as LDL(-) from NL showed a single band of lower size than non-electronegative LDL [LDL(+)], whereas LDL(-) from FH and HTG presented bands of greater size than its respective LDL(+). These results reveal the existence of light and dense LDL(-), indicate that hyperlipemia could promote the formation of light LDL(-) and suggest that LDL(-) could have different origins.

journal_name

J Lipid Res

authors

Sánchez-Quesada JL,Benítez S,Otal C,Franco M,Blanco-Vaca F,Ordóñez-Llanos J

keywords:

subject

Has Abstract

pub_date

2002-05-01 00:00:00

pages

699-705

issue

5

eissn

0022-2275

issn

1539-7262

journal_volume

43

pub_type

杂志文章
  • Quantitative analysis of uptake of free fatty acid by mammalian cells: lauric acid and human erythrocytes.

    abstract::Quantitative aspects of the binding of free fatty acid to human erythrocytes were studied by measuring the distribution of various amounts of [1-(14)C]lauric acid between washed human erythrocytes and defatted human plasma albumin. Incubations were done at 37 degrees C in an isotonic phosphate-buffered salt solution. ...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:

    authors: Spector AA,Ashbrook JD,Santos EC,Fletcher JE

    更新日期:1972-07-01 00:00:00

  • The role of innate immunity in atherogenesis.

    abstract::Lipid peroxidation is a common event in health and is greatly accelerated in pro-inflammatory settings such as hypercholesterolemia. Consequently, oxidation-specific epitopes are generated, which are pro-inflammatory and immunogenic, leading to both adaptive and innate responses. Because innate immune mechanisms use c...

    journal_title:Journal of lipid research

    pub_type: 杂志文章,评审

    doi:10.1194/jlr.R800100-JLR200

    authors: Hartvigsen K,Chou MY,Hansen LF,Shaw PX,Tsimikas S,Binder CJ,Witztum JL

    更新日期:2009-04-01 00:00:00

  • Fetal baboons convert 18:3n-3 to 22:6n-3 in vivo. A stable isotope tracer study.

    abstract::Using [13C]-tracers and direct fetal doses, we show for the first time that the fetal primate converts alpha-linolenic acid (18:3) to docosahexaenoic acid (22:6) in vivo, and we estimate the relative bioefficacy of the two substrates for brain 22:6 accretion. Pregnant female baboons consumed a diet free of long chain ...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:

    authors: Su HM,Huang MC,Saad NM,Nathanielsz PW,Brenna JT

    更新日期:2001-04-01 00:00:00

  • Subcellular localization and quantification of cholesterol in cultured human fibroblasts exposed to human low density lipoprotein.

    abstract::Subcellular localization of nonesterified cholesterol has been determined in normal human fibroblasts from cultures incubated with human low density lipoprotein (LDL). Nonesterified and esterified cholesterol content of fibroblasts, grown initially in the absence of cholesterol, increased significantly after a 1-hour ...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:

    authors: Kruth HS,Blanchette-Mackie J,Avigan J,Gamble W,Vaughan M

    更新日期:1982-11-01 00:00:00

  • Influence of lecithin acyl chain composition on the kinetics of exchange between chylomicrons and high density lipoproteins.

    abstract::The kinetics of lecithin exchange between native lipoproteins was characterized for individual molecular species of lecithins of rat mesenteric lymph chylomicrons and rat plasma HDL. Studies were performed in the absence of lipid transfer proteins. Donor (chylomicrons) and acceptor (HDL) particles were present in rati...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:

    authors: Patton GM,Robins SJ,Fasulo JM,Clark SB

    更新日期:1985-11-01 00:00:00

  • Regulation of the Ca(2+)-independent phospholipase A2 in liver mitochondria by changes in the energetic state.

    abstract::The effect of electron transport chain redox status on activity of the mitochondrial Ca(2+)-independent phospholipase A2 (iPLA2) has been examined. When oxidizing NAD-linked substrates, the enzyme is not active unless deenergization occurs. Uncoupler, rotenone, antimycin A, and cyanide are equally effective at upregul...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:10.1194/jlr.M043307

    authors: Rauckhorst AJ,Broekemeier KM,Pfeiffer DR

    更新日期:2014-05-01 00:00:00

  • Sphingomyelins and ceramides with VLCPUFAs are excluded from low-density raft-like domains in differentiating spermatogenic cells.

    abstract::Rat spermatogenic cells contain sphingomyelins (SMs) and ceramides (Cers) with very long-chain PUFAs (VLCPUFAs) in nonhydroxylated (n-V) and 2-hydroxylated (h-V) forms. How these atypical species distribute among membrane fractions during differentiation was investigated here using a detergent-free procedure to isolat...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:10.1194/jlr.M072595

    authors: Santiago Valtierra FX,Mateos MV,Aveldaño MI,Oresti GM

    更新日期:2017-03-01 00:00:00

  • Lrp13 is a novel vertebrate lipoprotein receptor that binds vitellogenins in teleost fishes.

    abstract::Transcripts encoding a novel member of the lipoprotein receptor superfamily, termed LDL receptor-related protein (Lrp)13, were sequenced from striped bass (Morone saxatilis) and white perch (Morone americana) ovaries. Receptor proteins were purified from perch ovary membranes by protein-affinity chromatography employi...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:10.1194/jlr.M050286

    authors: Reading BJ,Hiramatsu N,Schilling J,Molloy KT,Glassbrook N,Mizuta H,Luo W,Baltzegar DA,Williams VN,Todo T,Hara A,Sullivan CV

    更新日期:2014-11-01 00:00:00

  • microRNA-30c reduces plasma cholesterol in homozygous familial hypercholesterolemic and type 2 diabetic mouse models.

    abstract::High plasma cholesterol levels are found in several metabolic disorders and their reductions are advocated to reduce the risk of atherosclerosis. A way to lower plasma lipids is to curtail lipoprotein production; however, this is associated with steatosis. We previously showed that microRNA (miR)-30c lowers diet-induc...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:10.1194/jlr.M081299

    authors: Irani S,Iqbal J,Antoni WJ,Ijaz L,Hussain MM

    更新日期:2018-01-01 00:00:00

  • Lipoprotein lipase and hepatic lipase: their relationship with HDL subspecies Lp(A-I) and Lp(A-I,A-II).

    abstract::HDL subspecies Lp(A-I) and Lp(A-I,A-II) have different anti-atherogenic potentials. To determine the role of lipoprotein lipase (LPL) and hepatic lipase (HL) in regulating these particles, we measured these enzyme activities in 28 healthy subjects with well-controlled Type 1 diabetes, and studied their relationship wi...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:10.1194/jlr.M300091-JLR200

    authors: Cheung MC,Sibley SD,Palmer JP,Oram JF,Brunzell JD

    更新日期:2003-08-01 00:00:00

  • Improved method for the synthesis of 1- or 3-acyl-sn-glycerols.

    abstract::Optically active 1- or 3-acyl-sn-glycerols were synthesized from 2,3- or 1,2-isopropylidene-sn-glycerols, respectively. The 2,3- or 1,2-isopropylidene-sn-glycerols were condensed with appropriate long saturated or unsaturated fatty acids and the resulting acyl isopropylidene compounds were treated with dimethylboronbr...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:

    authors: Kodali DR

    更新日期:1987-04-01 00:00:00

  • n-3 PUFA dietary supplementation inhibits proliferation and store-operated calcium influx in thymoma cells growing in Balb/c mice.

    abstract::The antitumor effect of daily individual administration of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) (2 g/kg body weight) in Balb/c mice bearing a transplantable thymoma was investigated. Mice received oleic acid (control group), EPA and DHA ethyl esters starting 10 days before tumor inoculation. Anal...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:

    authors: Calviello G,Palozza P,Di Nicuolo F,Maggiano N,Bartoli GM

    更新日期:2000-02-01 00:00:00

  • Biomarkers of NAFLD progression: a lipidomics approach to an epidemic.

    abstract::The spectrum of nonalcoholic fatty liver disease (NAFLD) includes steatosis, nonalcoholic steatohepatitis (NASH), and cirrhosis. Recognition and timely diagnosis of these different stages, particularly NASH, is important for both potential reversibility and limitation of complications. Liver biopsy remains the clinica...

    journal_title:Journal of lipid research

    pub_type: 临床试验,杂志文章,多中心研究

    doi:10.1194/jlr.P056002

    authors: Gorden DL,Myers DS,Ivanova PT,Fahy E,Maurya MR,Gupta S,Min J,Spann NJ,McDonald JG,Kelly SL,Duan J,Sullards MC,Leiker TJ,Barkley RM,Quehenberger O,Armando AM,Milne SB,Mathews TP,Armstrong MD,Li C,Melvin WV,Clemen

    更新日期:2015-03-01 00:00:00

  • Trafficking of glycosylphosphatidylinositol anchored proteins from the endoplasmic reticulum to the cell surface.

    abstract::In eukaryotes, many cell surface proteins are attached to the plasma membrane via a glycolipid glycosylphosphatidylinositol (GPI) anchor. GPI-anchored proteins (GPI-APs) receive the GPI anchor as a conserved posttranslational modification in the lumen of the endoplasmic reticulum (ER). After anchor attachment, the GPI...

    journal_title:Journal of lipid research

    pub_type: 杂志文章,评审

    doi:10.1194/jlr.R062760

    authors: Muñiz M,Riezman H

    更新日期:2016-03-01 00:00:00

  • A rapid and quantitative LC-MS/MS method to profile sphingolipids.

    abstract::Sphingolipids comprise a highly diverse and complex class of molecules that serve not only as structural components of membranes but also as signaling molecules. To understand the differential role of sphingolipids in a regulatory network, it is important to use specific and quantitative methods. We developed a novel ...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:10.1194/jlr.D005322

    authors: Scherer M,Leuthäuser-Jaschinski K,Ecker J,Schmitz G,Liebisch G

    更新日期:2010-07-01 00:00:00

  • Four novel mutations of sterol 27-hydroxylase gene in Italian patients with cerebrotendinous xanthomatosis.

    abstract::We report the characterization of eight mutations of sterol 27-hydroxylase gene (CYP27) in five Italian patients with cerebrotendinous xanthomatosis, who were found to be compound heterozygotes. Four mutations (C --> T at nt 45 of exon 4, G(+1) --> A in intron 6, G(+5) --> T in intron 7, and G(-1) --> A in intron 7) a...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:

    authors: Garuti R,Croce MA,Tiozzo R,Dotti MT,Federico A,Bertolini S,Calandra S

    更新日期:1997-11-01 00:00:00

  • Measurement of cholic acid synthesis and secretion by isolated rat hepatocytes.

    abstract::Liver cells isolated from normal and cholestyramine-treated rats were incubated as cell suspensions for up to 4 hr in a simple, defined medium. The bile acid concentration in cells plus cell medium was determined by gas-liquid chromatography. Normal hepatocytes synthesized cholic acid at an initial rate of 0.25 nmol/m...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:

    authors: Whiting MJ,Edwards AM

    更新日期:1979-09-01 00:00:00

  • Eicosapentaenoic and docosahexaenoic acid ethyl esters differentially enhance B-cell activity in murine obesity.

    abstract::EPA and DHA are not biologically equivalent; however, their individual activity on B cells is unknown. We previously reported fish oil enhanced murine B-cell activity in obesity. To distinguish between the effects of EPA and DHA, we studied the ethyl esters of EPA and DHA on murine B-cell function as a function of tim...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:10.1194/jlr.M049809

    authors: Teague H,Harris M,Fenton J,Lallemand P,Shewchuk BM,Shaikh SR

    更新日期:2014-07-01 00:00:00

  • Dietary cholesterol opposes PUFA-mediated repression of the stearoyl-CoA desaturase-1 gene by SREBP-1 independent mechanism.

    abstract::Stearoyl-CoA desaturase (SCD) catalyzes the rate-limiting step in the cellular synthesis of monounsaturated fatty acids, mainly oleate (18:1) and palmitoleate (16:1), which are the major monounsaturated fatty acids of membrane phospholipids, cholesteryl esters, waxes, and triglycerides. The mouse expresses three well-...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:10.1194/jlr.m100433-jlr200

    authors: Kim HJ,Miyazaki M,Ntambi JM

    更新日期:2002-10-01 00:00:00

  • The separation of sterol intermediates in cholesterol biosynthesis by high pressure liquid chromatography.

    abstract::A three-step procedure has been developed for the separation of complex mixtures of sterol intermediates in cholesterol biosynthesis. The method has been applied to the separation of sterol intermediates formed from [14C]mevalonate by normal rat hepatocyte culture cells. In Step 1, a short gravity-flow silicic acid co...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:

    authors: Hansbury E,Scallen TJ

    更新日期:1980-09-01 00:00:00

  • Uptake of chylomicron remnants by the liver: further evidence for the modulating role of phospholipids.

    abstract::Rat lymph chylomicrons were treated with rat heparin-releasable hepatic lipase (HL) or with bovine milk lipoprotein lipase (LPL). The ability of the resulting particles to be taken up by the liver in vivo was assessed following their infusion into the portal vein of partially hepatectomized animals. The following obse...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:

    authors: Borensztajn J,Getz GS,Kotlar TJ

    更新日期:1988-08-01 00:00:00

  • Lower HDL-C and apolipoprotein A-I are related to higher glomerular filtration rate in subjects without kidney disease.

    abstract::Animal experiments show that the kidney contributes to apolipoprotein (apo)A-I catabolism. We tested relationships of HDL cholesterol (HDL-C) and apo-I with kidney function in subjects without severe chronic kidney disease. Included was a random sample of the general population (part of the PREVEND cohort). Kidney fun...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:10.1194/jlr.M005348

    authors: Krikken JA,Gansevoort RT,Dullaart RP,PREVEND Study Group.

    更新日期:2010-07-01 00:00:00

  • Metabolism and proteomics of large and small dense LDL in combined hyperlipidemia: effects of rosuvastatin.

    abstract::Small dense LDL (sdLDL) has been reported to be more atherogenic than large buoyant LDL (lbLDL). We examined the metabolism and protein composition of sdLDL and lbLDL in six subjects with combined hyperlipidemia on placebo and rosuvastatin 40 mg/day. ApoB-100 kinetics in triglyceride-rich lipoproteins (TRLs), lbLDL (d...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:10.1194/jlr.M073882

    authors: Thongtang N,Diffenderfer MR,Ooi EMM,Barrett PHR,Turner SM,Le NA,Brown WV,Schaefer EJ

    更新日期:2017-07-01 00:00:00

  • Resistin expression in 3T3-L1 adipocytes is reduced by arachidonic acid.

    abstract::The resistin gene is expressed in adipocytes and encodes a protein proposed to link obesity and type 2 diabetes. Increased plasma FFA is associated with insulin resistance. We examined the effect of separate FFAs on the expression of resistin mRNA in cultured murine 3T3-L1 adipocytes. The FFAs tested did not increase ...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:10.1194/jlr.M400348-JLR200

    authors: Haugen F,Zahid N,Dalen KT,Hollung K,Nebb HI,Drevon CA

    更新日期:2005-01-01 00:00:00

  • Reduced ileal expression of OSTalpha-OSTbeta in non-obese gallstone disease.

    abstract::Cholelithiasis is a multifactorial process, and several mechanisms have been postulated. A decreased expression of the ileal apical sodium-dependent bile acid transporter (ASBT) and of the cytosolic ileal lipid binding protein (ILBP) was recently described in female non-obese patients. The role of the recently identif...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:10.1194/jlr.M800162-JLR200

    authors: Renner O,Harsch S,Strohmeyer A,Schimmel S,Stange EF

    更新日期:2008-09-01 00:00:00

  • Prostaglandin synthesis in rat adrenocortical cells.

    abstract::The biosynthesis of prostaglandins by isolated rat adrenocortical cells has been studied by determinations of products formed during incubations with labeled arachidonic acid and by radioimmunoassays. Analysis by thin-layer chromatographic separation of silicic acid column fractions indicated that PGE2, PGA2, (B2) and...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:

    authors: Chanderbhan R,Hodges VA,Treadwell CR,Vahouny GV

    更新日期:1979-01-01 00:00:00

  • A newborn screening method for cerebrotendinous xanthomatosis using bile alcohol glucuronides and metabolite ratios.

    abstract::Cerebrotendinous xanthomatosis (CTX) is a treatable neurodegenerative metabolic disorder of bile acid synthesis in which symptoms can be prevented if treatment with chenodeoxycholic acid supplementation is initiated early in life, making CTX an excellent candidate for newborn screening. We developed a new dried blood ...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:10.1194/jlr.P075051

    authors: Vaz FM,Bootsma AH,Kulik W,Verrips A,Wevers RA,Schielen PC,DeBarber AE,Huidekoper HH

    更新日期:2017-05-01 00:00:00

  • Vitamin A transport in chicken plasma: isolation and characterization of retinol-binding protein (RBP), prealbumin (PA), and RBP--PA complex.

    abstract::Vitamin A-transporting protein in chicken plasma was purified by column chromatography on DEAE-Sephadex and Sephadex G-100; the protein formed a complex of retinol-binding protein (RBP) with prealbumin (PA). The molecular weight of the 1:1 molar complex was estimated to be 76,000 by gel filtration, and the sedimentati...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:

    authors: Abe T,Muto Y,Hosoya N

    更新日期:1975-05-01 00:00:00

  • Modulation of paraoxonase 1 and 3 expression after moderate exercise training in the rat.

    abstract::Paraoxonases (PONs) are a small family of antioxidant enzymes whose antiatherogenic activity is well known. The aim of the present study was the evaluation of the effects of moderate aerobic training on their expression using a rat model. In order to discriminate between PON1 and PON3 enzymatic activity, we took advan...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:10.1194/jlr.M800493-JLR200

    authors: Romani R,De Medio GE,di Tullio S,Lapalombella R,Pirisinu I,Margonato V,Veicsteinas A,Marini M,Rosi G

    更新日期:2009-10-01 00:00:00

  • Acetaldehyde adducts and autoantibodies against VLDL and LDL in alcoholics.

    abstract::Alcohol consumption markedly increases the hepatic output of very low density lipoprotein (VLDL), whereas it decreases the resulting low density lipoprotein (LDL) levels and apolipoprotein B. As ethylation of apoB-lysine renders LDL immunogenic and accelerates their clearance, and as alcoholics develop antibodies agai...

    journal_title:Journal of lipid research

    pub_type: 杂志文章

    doi:

    authors: Wehr H,Rodo M,Lieber CS,Baraona E

    更新日期:1993-07-01 00:00:00