Abstract:
:Fish nonspecific cytotoxic cells (NCC) lyse various transformed human B-cells (NC-37, P3HR-1) and erythroblastoid cells (K562) as well as mouse YAC-1 and P815 cells. Highest NCC activity was found in the anterior (head) kidney, but spleen cells and peripheral blood leucocytes (PBL) also demonstrated cytolytic abilities. Lysis of chromium-51 labeled target cells occurred rapidly and optimum cytolysis developed at either 16 degrees C or 26 degrees C incubation temperatures. Preincubation at temperatures of 4 degrees C or 37 degrees C for 4 hours reduced NCC cytotoxicity. Although catfish (Ictalurus punctatus) are extensively outbred, interfish NCC activities did not significantly vary at the optimum E:T ratios (160). The NCC target antigen specificities were partially determined by cold target inhibition (CTI) studies. YAC-1 and K562 did not produce significant CTI, however. These studies demonstrated the presence of a highly active cytotoxic cell which is widely distributed in fish lymphoreticular tissue. NCC kill divergent kinds of transformed cell types, and the target cell specificity for human transformed B-cells is different from the NCC target cell antigens on other human (K562) and on mouse (YAC-1 and P815) cells.
journal_name
Dev Comp Immunoljournal_title
Developmental and comparative immunologyauthors
Evans DL,Graves SS,Cobb D,Dawe DLdoi
10.1016/0145-305x(84)90037-5subject
Has Abstractpub_date
1984-04-01 00:00:00pages
303-12issue
2eissn
0145-305Xissn
1879-0089pii
0145-305X(84)90037-5journal_volume
8pub_type
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