Methylated bases in the host-modified deoxyribonucleic acid of Escherichia coli and bacteriophage lambda.

abstract：:Necrotic enteritis (NE) is an economically important disease of poultry caused by certain Clostridium perfringens type A strains. NE pathogenesis involves the NetB toxin, which is encoded on a large conjugative plasmid within a 42-kb pathogenicity locus. Recent multilocus sequence type (MLST) studies have identified t...

journal_title：Journal of bacteriology

authors： Lepp D,Gong J,Songer JG,Boerlin P,Parreira VR,Prescott JF

更新日期：2013-03-01 00:00:00

abstract：:The trfA gene of the broad-host-range plasmid RK2 is essential for initiation of plasmid replication. Two related TrfA proteins of 43 and 32 kilodaltons (kDa) are produced by independent translation initiation at two start codons within the trfA open reading frame. These proteins were o overproduced in Escherichia col...

journal_title：Journal of bacteriology

authors： Durland RH,Helinski DR

更新日期：1990-07-01 00:00:00

abstract：:Specific labeling of a single row of cellulose-synthesizing complexes (terminal complexes, TC subunits, TCs, or TC arrays) in Acetobacter xylinum by antibodies raised against a 93-kDa protein (the cyclic dignanylic acid-binding protein) has been demonstrated by using the sodium dodecyl sulfate (SDS)-freeze-fracture la...

journal_title：Journal of bacteriology

authors： Kimura S,Chen HP,Saxena IM,Brown RM Jr,Itoh T

更新日期：2001-10-01 00:00:00

abstract：:The lic3 locus of Haemophilus influenzae consists of four open reading frames. The derived amino acid sequences of orf2 and orf4 exhibit homology to Escherichia coli GalE and AdK, respectively. The functions of orf1 and orf3 remain unknown. orf1 contains multiple tandem repeats of the tetrameric DNA sequence CAAT near...

journal_title：Journal of bacteriology

authors： Szabo M,Maskell D,Butler P,Love J,Moxon R

更新日期：1992-11-01 00:00:00

abstract：:The alpha-aminoadipate pathway for lysine biosynthesis is present only in fungi. The alpha-aminoadipate reductase (AAR) of this pathway catalyzes the conversion of alpha-aminoadipic acid to alpha-aminoadipic-delta-semialdehyde by a complex mechanism involving two gene products, Lys2p and Lys5p. The LYS2 and LYS5 genes...

journal_title：Journal of bacteriology

authors： Guo S,Evans SA,Wilkes MB,Bhattacharjee JK

更新日期：2001-12-01 00:00:00

abstract：:Burkholderia thailandensis is a soil saprophyte that is closely related to the pathogen Burkholderia pseudomallei, the etiological agent of melioidosis in humans. The environmental niches and infection sites occupied by these bacteria are thought to contain only limited concentrations of oxygen, where they can generat...

journal_title：Journal of bacteriology

authors： Vitale A,Paszti S,Takahashi K,Toyofuku M,Pessi G,Eberl L

更新日期：2020-11-04 00:00:00

abstract：:During the growth of Clostridium cellulolyticum in chemostat cultures with ammonia as the growth-limiting nutrient, as much as 30% of the original cellobiose consumed by C. cellulolyticum was converted to cellotriose, glycogen, and polysaccharides regardless of the specific growth rates. Whereas the specific consumpti...

journal_title：Journal of bacteriology

authors： Guedon E,Desvaux M,Petitdemange H

更新日期：2000-04-01 00:00:00

abstract：:Quorum sensing control mediated by N-acyl homoserine lactone (AHL) signaling molecules has been established as a key feature of the regulation of exoenzyme production in many gram-negative bacteria. In Chromobacterium violaceum ATCC 31532 a number of phenotypic characteristics, including production of the purple pigme...

journal_title：Journal of bacteriology

authors： Chernin LS,Winson MK,Thompson JM,Haran S,Bycroft BW,Chet I,Williams P,Stewart GS

更新日期：1998-09-01 00:00:00

abstract：:Cysteine and methionine availability influences many processes in the cell. In bacteria, transcription of the specific genes involved in the synthesis of these two amino acids is usually regulated by different mechanisms or regulators. Pathways for the synthesis of cysteine and methionine and their interconversion wer...

journal_title：Journal of bacteriology

authors： Sperandio B,Polard P,Ehrlich DS,Renault P,Guédon E

更新日期：2005-06-01 00:00:00

abstract：:Activation (phosphorylation) of the transcription factor encoded by spo0A is essential for the initiation of sporulation in Bacillus subtilis. At least three histidine protein kinases are involved in the phosphorylation of Spo0A. Under some growth conditions, KinA was the primary kinase, but under other conditions, Ki...

journal_title：Journal of bacteriology

authors： LeDeaux JR,Yu N,Grossman AD

更新日期：1995-02-01 00:00:00

abstract：:The Salmonella typhimurium aroF gene, encoding the tyrosine-sensitive 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) synthase, was localized to a chromosomal PstI fragment by Southern blotting with an Escherichia coli aroF probe. This fragment was cloned by screening a plasmid library for complementation of an E. ...

journal_title：Journal of bacteriology

authors： Muday GK,Herrmann KM

更新日期：1990-05-01 00:00:00

abstract：:Hogg, Robert W. (University of Illinois, Urbana), Chitra S. Biswas, and Harry P. Broquist. Interference with valine and isoleucine biosynthesis by cyclic hydroxamic acids. J. Bacteriol. 90:1265-1270. 1965.-The introduction of a hydroxyl group in a number of common barbiturates, a substitution which converts these comp...

journal_title：Journal of bacteriology

authors： Hogg RW,Biswas CS,Broquist HP

更新日期：1965-11-01 00:00:00

abstract：:The genomic organization of Bordetella pertussis strains has been examined by using a new method. This method does not depend on the prior determination of a restriction map of the bacterial chromosome but is based on the ability to measure directly the distance between two genes. This is accomplished through the inte...

journal_title：Journal of bacteriology

authors： Stibitz S,Yang MS

更新日期：1997-09-01 00:00:00

abstract：:Nitrate reductase extracted from the membrane of Escherichia coli by alkaline heat treatment was purified to homogeneity and used to prepare specific antibody. Nitrate reductase, precipitated by this antibody from Triton extracts of the membrane, contained a third subunit not present in the purified enzyme used to pre...

journal_title：Journal of bacteriology

authors： MacGregor CH

更新日期：1975-03-01 00:00:00

abstract：:Although arsenic is highly toxic to most organisms, certain prokaryotes are known to grow on and respire toxic metalloids of arsenic (i.e., arsenate and arsenite). Two enzymes are known to be required for this arsenic-based metabolism: (i) the arsenate respiratory reductase (ArrA) and (ii) arsenite oxidase (AoxB). Bot...

journal_title：Journal of bacteriology

authors： Zargar K,Hoeft S,Oremland R,Saltikov CW

更新日期：2010-07-01 00:00:00

abstract：:Bacillus subtilis contains three Fur homologs: Fur, PerR, and Zur. Despite significant sequence similarities, they respond to different stimuli and regulate different sets of genes. DNA target site comparisons indicate that all three paralogs recognize operators with a core 7-1-7 inverted repeat. The corresponding con...

journal_title：Journal of bacteriology

authors： Fuangthong M,Helmann JD

更新日期：2003-11-01 00:00:00

abstract：:The first three enzymes of the pentachlorophenol (PCP) degradation pathway in Sphingobium chlorophenolicum (formerly Sphingomonas chlorophenolica) ATCC 39723 have been characterized, and the corresponding genes, pcpA, pcpB, and pcpC, have been individually cloned and sequenced. To search for new genes involved in PCP ...

journal_title：Journal of bacteriology

authors： Cai M,Xun L

更新日期：2002-09-01 00:00:00

abstract：:The Mg-2+-Sarkosyl crystals (M band) procedure was used to study the effect of ribonuclease (RNase) A on the association of Escherichia coli deoxyribonucleic acid (DNA) with membrane. Incubation of gently prepared cell extracts with RNase results in the release of DNA from membrane. This effect appears to result from ...

journal_title：Journal of bacteriology

authors： McIntosh MA,Earhart CF

更新日期：1975-05-01 00:00:00

abstract：:Studies of mitochondrial biogenesis in yeast have been hampered by a lack of suitable membrane markers in anaerobically grown cells subsequently grown in air. Cytochrome c peroxidase activity and subcellular location was studied to determine whether it would be a useful marker for an analysis of mitochondrial formatio...

journal_title：Journal of bacteriology

authors： Avers CJ

更新日期：1967-10-01 00:00:00

abstract：:When the crude ribosomal fraction of Saccharomyces cerevisiae was separated into "light" and "heavy" fractions, fatty acid synthetase was concentrated in the former, whereas acetyl-Coenzyme A synthetase, fatty acid "desaturase," and squalene oxidocyclase were found in the latter. The "desaturase" sedimented with the r...

journal_title：Journal of bacteriology

authors： Klein HP,Volkmann CM,Leaffer MA

更新日期：1967-07-01 00:00:00

abstract：:We describe the further utilization of a genetic screen that identifies mutations defective in the assembly of proteins into the Escherichia coli cytoplasmic membrane. The screen yielded mutations in each of the known genes encoding components of the E. coli signal recognition particle pathway: ffh, ffs, and ftsY, whi...

journal_title：Journal of bacteriology

authors： Tian H,Beckwith J

更新日期：2002-01-01 00:00:00

abstract：:Picolinic acid (PA) is a natural toxic pyridine derivative. Microorganisms can degrade and utilize PA for growth. However, the full catabolic pathway of PA and its physiological and genetic foundation remain unknown. In this study, we identified a gene cluster, designated picRCEDFB4B3B2B1A1A2A3, responsible for the de...

journal_title：Journal of bacteriology

authors： Qiu J,Zhao L,Xu S,Chen Q,Chen L,Liu B,Hong Q,Lu Z,He J

更新日期：2019-07-24 00:00:00

abstract：:Membrane vesicles were characterized for their ability to specifically bind [14C]glucose 6-phosphate. Membranes prepared from a strain carrying a ColE1 uhp hybrid plasmid showed significantly enhanced glucose 6-phosphate binding. It is hypothesized that glucose 6-phosphate binding to these membranes is due to a uhpR-d...

journal_title：Journal of bacteriology

authors： Goldenbaum PE,Farmer KS

更新日期：1980-04-01 00:00:00

abstract：:The cell envelope of gram-negative bacteria is responsible for many important biological functions: it plays a structural role, it accommodates the selective transfer of material across the cell wall, it undergoes changes made necessary by growth and division, and it transfers information about the environment into th...

journal_title：Journal of bacteriology

authors： Vadillo-Rodriguez V,Beveridge TJ,Dutcher JR

更新日期：2008-06-01 00:00:00

abstract：:The virA and virG gene products are required for the regulation of the vir regulon on the tumor-inducing (Ti) plasmid of Agrobacterium tumefaciens. VirA is a membrane-associated protein which is homologous to the sensor molecules of other two-component regulatory systems. We overproduced truncated VirA proteins in Esc...

journal_title：Journal of bacteriology

authors： Jin S,Roitsch T,Ankenbauer RG,Gordon MP,Nester EW

更新日期：1990-02-01 00:00:00

abstract：:Schlissel, Harvey J. (The University of Kansas, Lawrence), and C. P. Sword. Conditions affecting transformation of a group H streptococcus. J. Bacteriol. 92:1357-1363. 1966.-A defined transforming medium (DTM) containing buffer and 5 to 10 mug per ml of deoxyribonucleic acid was developed to study the physical and che...

journal_title：Journal of bacteriology

authors： Schlissel HJ,Sword CP

更新日期：1966-11-01 00:00:00

abstract：:An increased demand for cytochromes is associated with symbiotic development and microaerobic metabolism in the bacterium Bradyrhizobium japonicum, and evidence suggests that hemB, rather than hemA, is the first essential bacterial heme synthesis gene in symbiosis with soybean. Steady-state levels of mRNA and protein ...

journal_title：Journal of bacteriology

authors： Chauhan S,O'Brian MR

更新日期：1997-06-01 00:00:00

abstract：:Welker, N. E. (University of Illinois, Urbana), and L. Leon Campbell. Biochemical changes in lysogenic Bacillus stearothermophilus after bacteriophage induction. J. Bacteriol. 90:1129-1137. 1965.-Cultures of Bacillus stearothermophilus 1503-4R (TP-1) continued to grow at an unaltered rate after induction with mitomyci...

journal_title：Journal of bacteriology

authors： Welker NE,Campbell LL

更新日期：1965-10-01 00:00:00

abstract：:In coupling the reducing power of illuminated chloroplasts to the nitrogenase from photosynthetically grown Rhodospirillum rubrum cells, one of the native ferredoxins. FdI, was found to be three times more effective than FdII. ...

journal_title：Journal of bacteriology

authors： Yoch DC,Arnon DI

更新日期：1975-02-01 00:00:00

abstract：:We examined the predacious gram-negative bacterium Bdellovibrio bacteriovorous 109J and free-living strains 109J-A1 and 109J-KA1 derived therefrom for penicillin-binding proteins (PBPs). We compared their PBPs with those of the host bacterium, Escherichia coli, and with those of a facultatively predacious bdellovibrio...

journal_title：Journal of bacteriology

authors： Park JT,Mahadevan S

更新日期：1988-08-01 00:00:00